• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.131-136
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• 1994

In the present study, in order to make an in vitro model of gastric mucosa for physiological and pharmacological studies, we established two immortalized gastric surface mucous cell lines (GSM06 and GSM10), which produce periodic acid-Schiff (PAS)-and concanavalin A (Con A)-positive glycoproteins, from a primary culture of gastric fundic mucosal cells of adult transgenic mice harboring a temperature-sensitive simian virus 40 large T-antigen gene 〔1]. Gastric fundic mucosal cells were isolated as a modification of a previously described method for rats by Schepp et al. (2). The isolated gastric fundic mucosal cells were cultured in DME/F12 medium supplemented with 2% fetal bovine serum (FBS), 1% ITES (consisting of 2 mg/1 insulin, 2 mgg/1 transferrin, 0.122 mg/1 ethanolamine and 0.00914 mg/1 sodium selenite) and 10 ng/ml recombinant epidermal growth factor (EGF) in a collagen-coated culture dish. To remove fibroblastic cells from the culture, gastric mucosal cells were incubated in the culture medium containing dispase (25 U/ml) for 24 h. The cells, uncontaminated with fibroblastic cells, were then cloned by colony formation. In our series of three attempts, two cell lines (GSM06 and GSM10) have been established at last. The cells proliferated, attached to the dish ana grew until confluent monolayers were formed, and maintained tight contact with neighboring cells. Both GSM06 and GSM10 cells have now been in culture for more than 9 months with regular passaging. The either cell produced

• Progress in Medical Physics
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• v.11 no.1
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• pp.19-27
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• 2000

In order to predict the propagation of road traffic noise over level terrain, this paper presents the model of the simple monopole and dipole source. Excess attenuations by ground acoustic impedance with continuity are investigated by outdoor experiments. In this results, the propagation of road traffic noise is affected by the acoustic impedance properties of the ground surface over which the noise travels, particularly when the receiver position is close to the surface. These results are then used to derive sound pressure level contours for the attenuation with distance of the equivalent energy level received from passing vehicle. The percentage of deep sleep and the number of wakings are studied for continuous traffic noise. The hearing loss and sensitivity threshold shift is investigated by changing the various parameters such as the effects of aging and noise exposure. Evaluation of the response of human to traffic noise is discribed. It is shown that exposure to excessive noise can cause temporary loss of hearing that may become permanent if the exposure is prolonged or intense.

• Journal of Forest and Environmental Science
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• v.33 no.2
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• pp.113-121
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• 2017

In flight particulate matter particularly emissions generated by incomplete combustion processes has become a subject of global concern due to the health problems and environmental impacts associated with them. This has compelled most countries to set standards for coarse and fine particles due to their conspicuous impacts on environment and public health. This contribution therefore explores forest fire emissions and how its particulates affects air quality, damage to vegetation, water bodies and biological functions as architects for lung diseases and other degenerative illnesses such as oxidative stress and aging. Soot was collected from simulated forest fire using a clean glass surface and carefully transferred into amber vials for analysis. Volatile components of soot were collected over 10 mL dichloromethane and analyzed using a QTOF Premier-Water Corp Liquid Chromatography hyphenated to a mass selective detector (MSD), and Gas Chromatograph coupled to a mass spectrometer (GC-MS). To characterize the size and surface morphology of soot, a scanning electron microscope (SEM) was used. The characterization of molecular volatiles from simulated forest fire emissions revealed long chain compounds including octadec-9-enoic acid, octadec-6-enoic acid, cyclotetracosane, cyclotetradecane, and a few aromatic hydrocarbons (benzene and naphthalene). Special classes of organics (dibenzo-p-dioxin and 2H-benzopyran) were also detected as minor products. Dibenzo-p-dioxin for instance in chlorinated form is one of the deadliest environmental organic toxins. The average particulate size of emissions using SEM was found to be $11.51{\pm}4.91{\mu}m$. This study has shown that most of the emissions from simulated forest fire fall within $PM_{10}$ particulate size. The molecular by-products of forest fire and particulate emissions may be toxic to both human and natural ecosystems, and are possible precursors for various respiratory ailments and cancers. The burning of a forest by natural disasters or man-made fires results in the destruction of natural habitats and serious air pollution.

• Tunnel and Underground Space
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• v.27 no.6
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• pp.387-392
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• 2017

Recently, road cave-ins, also referred to as ground sinking, have become a problem in urban environments. Public utility facilities such as sewage pipelines, communications pipes, gas pipes, power cables, and other types of underground structures are installed below the roads. It was reported that cave-ins are caused by the aging and lack of proper maintenance of underground facilities, as well as by construction problems. A road cave-in is first initiated by the formation of cavities typically induced by the breakage of underground pipelines. The cavities then grow and reach the base of the pavement. The traffic load applied at the surface of the roads causes an abrupt plastic deformation. This type of accident can be considered as a type of disaster. A road cave-in can threaten both human safety and the economy. It may even result in the loss of human life. In the city of Seoul, efforts to prevent damage before cave-ins occur have been prioritized, through a method of discovering and repairing joints through the 3D GPR survey.

• Journal of Soil and Groundwater Environment
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• v.16 no.6
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• pp.79-94
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• 2011

Black carbon (BC), a kind of high surface area carbonaceous material (HSACM), was isolated from Andong lake sediment. Sorption and desorption kinetics of naphthalene (Naph) and phenanthrene (Phen) in organic carbon (OC) and BC in the Andong lake sediment were investigated. Several kinetic models such as one-site mass transfer model (OSMTM), two-compartment first-order kinetic model (TCFOKM), and a newly proposed modified two-compartment first-order kinetic model (MTCFOKM) were used to describe the sorption and desorption kinetics. The MTCFOKM was the best fitting model. The MTCFOKM for sorption kinetics showed that i) the sorbed amounts of PAHs onto BC were higher than those onto OC, consistent with BET surface area; ii) the equilibration time for sorption onto BC was longer than those onto OC due to smaller size of micropore ($11.67{\AA}$) of BC than OC ($38.18{\AA}$); iii) initial sorption velocity of BC was higher than OC; and iv) the slow sorption velocity in BC caused the later equilibrium time than OC even though the fast sorption velocity was early completed in both BC and OC. The MTCFOKM also described the desorption of PAHs from the OC and BC well. After desorption, the remaining fractions of PAHs in BC were higher than those in OC due to stronger PAHs-BC binding. The remaining fractions increased with aging for both BC and OC.

• Proceedings of the KWS Conference
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• 2009.11a
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• pp.217-220
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• 2009

Conductive adhesives have been investigated for use in microelectronics packaging as a lead-free solder substitute due to their advantages, such as low bonding temperature. However, high resistivity and poor mechanical behavior may be the limiting factors for the development of conductive adhesives. The metal fillers and the polymer resins provide electrical and mechanical interconnections between surface mount device components and a substrate. As metal fillers used in conductive adhesives, silver is the most commonly used due to its high conductivity and the stability. However the cost of conductive adhesives with silver fillers is much higher than usual lead-free solders and silver has poor electro-migration performance. So, copper can be a promising candidate for conductive filler metal due to its low resistivity and low cost, but oxidation causes this metal to lose its conductivity. In this study, electrically conductive adhesives (ECAs) using surface modified copper fillers were developed. Especially, in order to overcome the problem associated with the oxidation of copper, copper particles were coated with silver, and the silver-coated copper was tested as a filler metal. Especially the effect of silver coating on the electrical resistance just after curing and after aging was investigated. As a result, it was found that the electrical resistance of ECA with silver-coated copper filler was clearly lower and more stable than that of ECA with pure copper filler after curing process. And, during high temperature storage test, the degradation rate of electrical resistance for ECA with silver coated copper filler was quite slower than that for ECA with pure copper filler.

• Journal of the Architectural Institute of Korea Planning & Design
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• v.36 no.4
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• pp.71-81
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• 2020

The purpose of this study is to propose the planning methods for pollution prevention in the initial design stage by identifying the pollution phenomena on the exterior of the building. It is important to catch the problem early on because exterior troubles begin with an initial design defect and causes rapid aging. The scope of this study is seoul public borough offices that should be sustainable for long as landmark located at the center of the district. Field survey was conducted three times in six months. Twenty-six borough offices were diagnosed with three levels of contamination through visual inspection and the contaminated locations, types, materials, and characteristics were checked. Fourteen common contaminated locations were identified in the first and intensively rechecked in the second. Five locations vulnerable to contamination were designated and the contamination and cleanliness situations were checked by location in the third. As a result, main polluted types and locations are sloping wall, upper wall, protruded wall, canopy, and wall under window. The key to avoiding these pollution problems is to minimize the horizontal plane on which dust can be deposited. In other words, it can be considered variously by shape and location, such as the slope of the horizontal plane, the floating of the surface, the installation of the roof surface, and the vertical louvers. And throating plans and material plans suitable for each location are presented. Various methods of the shape and location, material are expected to use as useful basic data for preventing pollutions during the initial design stage.

• The Research Journal of the Costume Culture
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• v.29 no.3
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• pp.361-373
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• 2021

The purpose of this study is to contribute to the role of lines in creative design development by analyzing the expression types and aesthetic characteristics of modern fashion using geometric formativeness of symmography. A literature study was conducted of works since 2009 to examine the general consideration of lines together with analysis of the concept and characteristics of symmography in the formative arts field, and to analyze the expression types and aesthetic characteristics of modern fashion design using the formativeness of symmography. The infinite sense of formativeness and original expression of symmography are used in formative arts such as space design, installation art, and industrial design. Expression types of modern fashion design using geometric formativeness of symmography can be classified into the following three types: two-dimensional graphic pattern, relief surface, and three-dimensional spatial. First, the two-dimensional graphic pattern type forms an optical pattern, providing individuality and visual interest to the textile design. Second, the relief surface type expresses the plane in various ways, so that the thickness changes according to how lines overlap. Third, the three-dimensional spatial type expands the boundaries of clothing and creates a fantastic spatial beauty. Next, the aesthetic formativeness of fashion design using symmography can be classified into repetitive rhythmicity, geometric self-similarity, and optical spatiality. Symmography enables a myriad of geometric patterns to be developed depending on material, color, and the designer's imagination, and helps inspire a variety of designs in fashion that sculpt a three-dimensional human body.

• Safety and Health at Work
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• v.11 no.4
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• pp.405-417
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• 2020

Background: Electrical socket outlets are used continuously until a failure occurs because they have no indication of manufacturing date or exchange specifications. For this reason, 659 electrical fires related to electrical socket outlets broke out in the Republic of Korea at 2018 only, an increase year on year. To reduce electrical fires from electrical socket outlets, it is necessary to perform an accelerated test and analyze the thermal, insulation resistance, and material properties of electrical socket outlets by installation years. Methods: Thermal characteristics were investigated by measured the temperature increase of electrical socket outlets classified according to year with variation of the current level. Insulation resistance characteristics was measured according to temperature for an electrical socket outlets by their years of use. Finally, to investigate the thermal and insulation resistance characteristics in relation to outlet aging, this study analyzed electrical socket outlets' conductor surface and content, insulator weight, and thermal deformation temperature. Results: Analysis showed, regarding the thermal characteristics, that electrical socket outlet temperature rose when the current value increased. Moreover, the longer the time that had elapsed since an accelerated test and installation, the higher the electrical socket outlet temperature was. With respect to the insulation resistance properties, the accelerated test (30 years) showed that insulation resistance decreased from 110 ℃. In relation to the installation year (30 years), insulation resistance decreased from 70 ℃, which is as much as 40 ℃ lower than the result found by the accelerated test. Regarding the material properties, the longer the elapsed time since installation, the rougher the surface of conductor contact point was, and cracks increased. Conclusion: The 30-year-old electrical socket outlet exceeded the allowable temperature which is 65 ℃ of the electrical contacts at 10 A, and the insulation resistance began to decrease at 70 ℃. It is necessary to manage electrical socket outlets that have been installed for a long time.

• Transactions on Control, Automation and Systems Engineering
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• v.4 no.1
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• pp.49-55
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• 2002

A decrease in the birthrate and aging are progressing in Japan and several countries. In that society, it is important that physically weak persons such as elderly persons are able to take care of themselves. We have been developing exoskeletal robots for human (especially for physically weak persons) motion support. In this study, the controller controls the angular position and impedance of the exoskeltal robot system using multiple fuzzy-neuro controllers based on biological signals that reflect the human subject's intention. Skin surface electromyogram (EMG) signals and the generated wrist force by the human subject during the elbow motion have been used as input information of the controller. Since the activation level of working muscles tends to vary in accordance with the flexion angle of elbow, multiple fuzzy-neuro controllers are applied in the proposed method. The multiple fuzzy-neuro controllers are moderately switched in accordance with the elbow flexion angle. Because of the adaptation ability of the fuzzy-neuro controllers, the exoskeletal robot is flexible enough to deal with biological signal such as EMG. The experimental results show the effectiveness of the proposed controller.