• Title/Summary/Keyword: supercritical carbon dioxide extraction

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Cholesterol Removal from Milk Fat by Supercritical Carbon Dioxide Extraction in coupled with Adsorption (초임계 이산화탄소 추출 및 흡착에 의한 유지방중의 콜레스테롤 제거)

  • Lim, Sang-Bin;Jwa, Mi-Kyung;Kwak, Hae-Soo
    • Korean Journal of Food Science and Technology
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    • v.30 no.3
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    • pp.574-580
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    • 1998
  • The technical feasibility of removing cholesterol from milk fat by supercritical carbon dioxide $(SC-CO_2)$ extraction followed by adsorption on different adsorbents and of fractionating milk fat into different fatty acid composition at $40^{\circ}C/276$ bar was investigated. Cholesterol could be selectively removed from milk fat by adsorption on a typical commercial florisil with $SC-CO_2$ extraction. Lower weight ratio of milk fat feed to florisil showed higher reduction of cholesterol, but gave lower yield in the milk fat fractions. The effective capacity of florisil for removing cholesterol from milk fat was 2.0g/g, which is the ratio of the fat feed to the adsorbent for 89% cholesterol reduction with a fat yield of 57.5%. Fatty acid composition showed higher short-chain and lower unsaturated long-chain fatty acids in the extracted fractions. Milk fat fractionation method by supercritical fluid extraction in coupled with adsorption would appear suitable for removing undesirable ingredients such as cholesterol and for enriching short-chain fatty acids in the fractions.

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Separation of Volatile Compounds from Tuna Fish Oil With Supercritical Carbon Dioxide (초임계 이산화탄소를 이용한 참치유로부터 휘발성 성분의 분리)

  • Roh Hyung Seob;Youn Hyun Seok;Jung Sun Mi;Hong Yeon Ryun;Kang Kil Yoon;Chun Byung Soo
    • KSBB Journal
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    • v.20 no.1 s.90
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    • pp.12-17
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    • 2005
  • Despite a wide range of fatty acids in fish oil, its the usage are very limited owing to off-flavors and volatile compounds in the oil. A way to extract and remove volatile compounds was performed at a semi-flow extractor by using supercritical carbon dioxide $(SC-CO_2)$. Samples of the oil were treated at the conditions which ranged from $30\;to\;80^{\circ}C$ and from 80 to 200 bar with 10 mL/min flow rate of carbon dioxide. In the oil the volatile compounds were analyzed by gas chromatography. Before extraction with $SC-CO_2$ the oil sample was detected over 129 peaks but 99 compounds were identified. The results demonstrated that at $40^{\circ}C$ and 200 bar extraction condition the volatile compounds in the tuna fish oil were removed, except for 14 compounds identified after extraction and other $SC-CO_2$ extraction conditions reached to high reduction of the volatile compounds.

Extraction Efficiencies of Organophosphorus Pesticides Spiked in Fish Tissues by Supercritical Carbon Dioxide (초임계 이산화탄소에 의한 어류조직 중 유기인계 농약의 첨가회수율)

  • Lim, Sang-Bin;Jwa, Mi-Kyung
    • Korean Journal of Food Science and Technology
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    • v.30 no.5
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    • pp.1163-1168
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    • 1998
  • Fish tissues were spiked with organophosphorus pesticides (OPPs), mixed with a celite as a drying agent, and dynamically extracted with pure $CO_2$ or modified $CO_2$ for 10 min at different extraction temperatures, pressures and $CO_2$ flow rates. Recoveries of OPPs spiked in jacopever increased with the decrease of extraction temperature and pressure, and decreased with the increase of $CO_2$ flow rates. Modified $CO_2$ extractions with 10% methylene chloride showed a slight increase in the recoveries over pure $CO_2$ extraction. Quantity of fish tissues had great effect on their extraction efficiencies. Recoveries of OPPs were $66.7{\sim}86.3%$ for jacopever, $56.2{\sim}79.2%$ for yellow tail, $57.6{\sim}77.8%$ for blanquillo, $84.2{\sim}96.3%$ for sardine, $74.6{\sim}83.6%$ for mackerel. Application of supercritical carbon dioxide extraction offers an attractive alternative to the use of organic solvents for extraction of pesticide residues from fish tissues.

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Extraction of Taxol and Baccatin III from Needles of Taxus Cuspidata by Using Supercritical Carbon Dioxide with Cosolvents (초임계 이산화탄소와 보조용매를 이용한 주목잎에서의 Taxol과 Baccatin III의 추출)

  • 신혜원;전문균이훈
    • KSBB Journal
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    • v.11 no.1
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    • pp.100-106
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    • 1996
  • The extraction of taxol and baccatin III from the ground needles of Taxus cuspidata were carried out by using supercritical carbon dioxide with and without cosolvents such as ethylacetate and methanol at 300 bar and 313K. Taxol is a promising anticancer agent and baccatin III is a precursor of semisysthesis of taxol. The taxol and baccatin III contents in the extracts were determined by a HPLC. The highest yields of taxol and baccatin III could be obtained by the supercritical extraction with 3wt% methanol and ethylacetate, respectively, as cosolvents. It was also found that the selectivity of taxol and baccatin III were 0.117 and 1.245wt%, respectively, with 0.7wt% ethylacetate, which demonstrated that the selectivity of taxol and baccatin III were increased about 1.8 and 19 times than those of conventional organic solvent extraction.

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Supercritical $CO_2$ Extraction of Genistein from Soybean (초임계 $CO_2$를 이용한 대두 Genistein의 추출)

  • Bu, Seong-Jun;Byeon, Sang-Yo
    • KSBB Journal
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    • v.14 no.4
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    • pp.490-494
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    • 1999
  • This study was carried out to examine some factors affecting the supercritical carbon dioxide extraction of genistein from soybean. The factors investigated in this study were pressure, temperature, flow rate of carbon dioxide, and concentration of modifier. It was foumd out that genistein is not extracted in the absence of modifier. Ethanol was found to be more effective modifier than methanol. 70% of genistein was extracted at 35$^{\circ}C$, 300bar and ethanol 15% (w/v) as compared with the performances of organic solvent extraction.

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Extraction of Pigment from Sea Mustard ( Undaiia pinnatinda) using Supercritical Carbon Dioxide and Entrainer (초임계 이산화탄소와 보조용매를 이용한 미역으로부터 색소 추출)

  • HONG Seok-Ki;CHUN Byung-Soo;PARK Sun-Young
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.3
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    • pp.213-217
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    • 2001
  • In order to develop a new separation technology, supercritical fluid extraction process was used to produce high purity pigments and fatty acids from seaweed (Undaria pinnatifida). Supercritical carbon dioxide was used as a solvent and ethanol as an entrainer. The sample was treated by a frozen drier and experiments were conducted with a semi-batch flow system at various operating conditions (pressure range, $10.3\~17.2$ MPa; temperature range, $30\~45^{\circ}C$: particle size, $500\~1,000{\mu}m$ extraction time, 60 min). Characteristics of the recovered pigment (chlorophyll a) and fatty acids were determined by UV-spectrophotometry and gas chromatography, respectively. The highest extraction efficiency for fatty acids and pigments was achieved at 12.4 MPa, $35^{\circ}C$, $500{\mu}m$of seaweed size.

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Fatty Acid Composition and Stability of Extracted Mackerel Muscle Oil and Oil-Polyethylene Glycol Particles Formed by Gas Saturated Solution Process

  • Haque, A.S.M. Tanbirul;Asaduzzaman, A.K.M.;Chun, Byung-Soo
    • Fisheries and Aquatic Sciences
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    • v.17 no.1
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    • pp.67-73
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    • 2014
  • The oil in mackerel muscle was extracted using an environment friendly solvent, supercritical carbon dioxide (SC-$CO_2$) at a semibatch flow extraction process and an n-hexane. The SC-$CO_2$ was maintained at a temperature of $45^{\circ}C$ under pressures ranging from 15 to 25 MPa. The flow rate of $CO_2$ (27 g/min) was constant during the entire 2 h extraction period. The fatty acid composition of the oil was analyzed using gas chromatography (GC). Significant concentrations of eicosapentaenoic acid (EPA) acid and docosahexaenoic acid (DHA) acid were present in the SC-$CO_2$ extracted oil. The oil extracted using SC-$CO_2$ exhibited increased stability compared with n-haxane extracted oil. Particles of mackerel oil together with the biodegradable polymer, polyethylene glycol (PEG) were formed using a gas saturated solution process (PGSS) with SC-$CO_2$ in a thermostatted stirred vessel. Different temperatures ($45-55^{\circ}C$), pressures (15-25 MPa) and a nozzle size $400{\mu}m$ were used for PGSS with a 1 h reaction time. The stability of mackerel oil in the particles did not changed significantly.

Synergistic Antifungal Activity of Phellodendri Cortex and Magnoliae Cortex against Candida albicans

  • NA, Hyunjeong;KIM, Tae-Jong
    • Journal of the Korean Wood Science and Technology
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    • v.50 no.1
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    • pp.12-30
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    • 2022
  • Many studies on plant extracts have been reported for the treatment of candidiasis caused by Candida albicans, a representative fungal infection. This study demonstrates the synergistic antifungal activity of the combination of Phellodendri Cortex and Magnoliae Cortex, previously reported to have antifungal efficacy. Considering the antifungal efficacy and the separation of the active constituents, berberine and magnolol, hot water extraction and carbon dioxide supercritical extraction were selected for Phellodendri Cortex and Magnoliae Cortex, respectively. A combination of 0.55 g/L hot water extract of Phellodendri Cortex and 0.59 g/L carbon dioxide supercritical extract of Magnoliae Cortex showed synergistic antifungal activity. The synergistic antifungal activity of 160 μM berberine and 100 μM magnolol, which are representative antifungal compounds of Phellodendri Cortex and Magnoliae Cortex, respectively, contributes to the synergistic antifungal effect of their extracts. The additive decrease in cellular ergosterol level and the increased antifungal efficacy by extracellular ergosterol suggest that disruption of the biological function of ergosterol in the cell membrane is not responsible for the synergistic antifungal activity of berberine and magnolol. Synergistic cellular release of chromosomal DNA upon mixing berberine and magnolol indicates that disruption of the cellular structure is responsible for the synergistic antifungal effect of berberine and magnolol.

Preparation of Gemcitabine-Loaded PLLA/Gemcitabine-PLLA Microparticles Using Supercritical Fluid and Their Release Characteristics (초임계 유체를 이용한 젬시타빈 함유 PLLA/젬시타빈-PLLA 미립자의 제조 및 방출 특성)

  • Hong, Jin-Hee;Lim, Giobin;Ryu, Jong-Hoon
    • KSBB Journal
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    • v.29 no.6
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    • pp.405-413
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    • 2014
  • In this study, gemcitabine (Gem)-Poly(L-lactic acid) (PLLA) conjugates were synthesized through an amide linkage reaction. Then, the microparticles of Gem-PLLA/PLLA blends containing gemcitabine were prepared using a supercritical fluid process, called aerosol solvent extraction system (ASES). Gemcitabine-loaded Gem-PLLA/PLLA microparticles obtained from the ASES process showed a spherical shape. The amount of gemcitabine released after 30 day incubation in a phosphate buffer solution of pH 7.4 was about 90% of the total amount of gemcitabine present in the product.

Tow-stage Extraction of Milk Fat by Supercritical Carbon Dioxide

  • Sangbin Lim;Jwa, Mi-Kyung;Kwak, Hae-Soo
    • Preventive Nutrition and Food Science
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    • v.2 no.3
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    • pp.202-206
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    • 1997
  • To develop mil fat fractions with desirable physico-chemical properties, anhydrous milk fat (AMF) was fractionated by one- and two-stage extractions using supercritical $CO_2$(SC-$CO_2$). Tow-stage extraction of AMF was performed by first producing tow fractions, an extract and a residue at 4$0^{\circ}C$/241bar, which were subsequently used as the feed for an extraction at 6$0^{\circ}C$/241bar and 4$0^{\circ}C$/345bar, and separated into five and four fractions, respectively, based one extraction time. These fractions were quantified and analyzed for fatty acids and physico-chemical properties. SHort-chain (C4~C8) fatty acids in extract fractions from an extract were 200~150% compared with those of the original AF. Long-chain (C14~C18) fatty acids in extract fractions from a residue were 118~141%. The ratio of unsaturated fatty acids in the residue fraction was 131%. Melting point ranged from 22 to 43$^{\circ}C$, iodine value 21.8 to 36.9, and saponification value 255 to 221 in the extract and residue fractions. SC-$CO_2$ fractionation of AMF by two-stage extraction offers the possibility of developing ractions with discrete fatty acid compositions and physico-chemical properties such as melting point, iodine value and saponification value.

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