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검색결과 956건 처리시간 0.025초

Expression of Thermostable $\alpha$-Glucosidase from Thermus caldophilus GK24 in Recombinant Saccharomyces cerevisiae

  • Choi, Jae-Youl;Ahn, Jung-Oh;Kim, Sun-Il;Shin, Hyun-Jae
    • Journal of Microbiology and Biotechnology
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    • 제16권12호
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    • pp.2000-2003
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    • 2006
  • A gene (GenBank AF096282) coding for a $\alpha$-glucosidase (TcaAG, EC 3.2.1.20) from Thermus caldophilus GK24 was expressed in Saccharomyces cerevisiae, a generally recognized as safe (GRAS) host. The thermostable $\alpha$-glucosidase was produced inside of the GRAS host at 0.04 unit/mg-dry cell by the constitutively expressing ADH1 promoter and at 1.2 unit/mg-dry cell by the inductively expressing GALl0 promoter, respectively. No $\alpha$-glucosidase activities were found in the medium when the MF-alpha signal sequence from S. cerevisiae or $\alpha$-amylase signal sequence from Aspergillus oryzae were fused before the $\alpha$-glucosidase gene for the secretion.

농업용수 수질기준 T-P 항목에 대한 검증실험(I) (An Evaluation study on Total Nitrogen(T-P) Item of Agricultural Water Standards)

  • 최선화;김호일;윤경섭;이봉훈
    • 한국농공학회:학술대회논문집
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    • 한국농공학회 2003년도 학술발표논문집
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    • pp.603-606
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    • 2003
  • This study was carried out to investigate the effects of T-P concentrations in irrigation water on the growth, yield, and grain quality of rice. It acquire fundamental knowledges to set up irrigation water quality standards. The pot experiment was conducted with 5 treatments using irrigation waters. It consisted of various total phosphorus concentrations(control, 1, 2, 5, 10mg/L) and replicated four times with randomized block design. The results of this study showed that as T-P concentrations in irrigation water increases, plant height tended to increase. Dry weight of rice plant at T-P 10mg/L was significantly higher than the control. However, T-P in irrigation water did not affect plant height, tiller number, plant dry weight, yield, and quality of rice.

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고강도 콘크리트의 자기수축 및 건조수축특성 평가 (The Evaluation of Properties on Autogenous Shrinkage and Dry Shrinkage of High Strength Concrete)

  • 이웅종;엄태선;이종열;곡촌충
    • 한국콘크리트학회:학술대회논문집
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    • 한국콘크리트학회 2006년도 춘계 학술발표회 논문집(II)
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    • pp.485-488
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    • 2006
  • The shrinkage properties of the high strength concrete using the cement of Type I, Type III and Type IV was examined, and the following results were obtained. (1) Consideration of the autogenous shrinkage when evaluating appropriately the shrinkage properties of the high strength concrete is indispensable. (2) The autogenous shrinkage prediction expression of JSCE can estimate the properties of autogenous shrinkage of the cement made from korea with in general sufficient accuracy. (3) It is necessary to advance examination which used Korean aggregate about dry shrinkage from now on, and to attain highly accuracy of the autogenous shrinkage prediction expression.

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무인기관실에 효과적인 자동소화장치개발 관한 연구 (A Study on the Development of Fire Extinguishing System for Machinery Spaces of a Small craft)

  • 이찬재;강대선;김동석;곽지현
    • 한국마린엔지니어링학회:학술대회논문집
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    • 한국마린엔지니어링학회 2006년도 전기학술대회논문집
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    • pp.127-128
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    • 2006
  • A study developing the dry powder fire extinguishing system inside the simulated machinery spaces of small boats was performed. Fire tests were conducted inside the compartments having volumes 2.9, 4.5, $8m^3$ respectively. The openings and fans were established on the walls of the compartments. Diesel oil was used for the test fuel, In addition fire extinguishing nozzles using dry powder were installed downward at ceiling and horizontally at the wall or conner. All fires in the test were extinguished under system activation and there was no reignition.

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Effects of Sowing Date on Growth and Yield of Schizonepeta tenuifolia Briquet in Southern Part of Korea

  • Park, Hee-Jin;Kwon, Byung-Sun
    • Plant Resources
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    • 제4권2호
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    • pp.97-101
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    • 2001
  • This study was carried out to determine the effect of sowing time on the flowering, growth and yield of Schizonepeta tenuifolia Briquet. Emergence and flowering dates in the sowing time from March 30 to April 30 were earlier than those of the other sowing times. In the sowing time from March 30 to April 30, length and diameter of main stem, number of node per main stem, number of branch per plant and fresh, and dry weight of stem were greater than those of the other sowing times. Yield components such as ear length, main stem length and diameter, branches per plant, number of node and ears per plant, yield of stem in fresh and dry were the highest at the sowing time from March 30 to April 30. Optimum sowing time of Schizonepeta tenuifolia Briquet were shown to be from March 30 to April 30 in southern areas of Korea.

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Effects of Sowing Amounts and Treatments on Agronomic Characteristics and Yield in Schizonepeta tenuifolia Briquet in Southern Part of Korea

  • Kwon, Byung-Sun;Park, Hee-Jin
    • Plant Resources
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    • 제4권2호
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    • pp.92-96
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    • 2001
  • seeding rates(1.5,2.0,3.0 and 3.5$\ell$/10a). There were no differences in the yield components such as length of stem and ear diameter of stem number of branch, internode and ear, and yield of fresh and dry stem between the seeding methods with drilling and broadcasting, but yield components such as length of stem and ear, diameter of stem, number of branch, internode and ear, and yield of fresh and dry stem increased with seeding rates of from 2.5$\ell$ /10a to 3.5$\ell$/10a. Therefore potimum rates and methods of sowing were from 2.5$\ell$/10a to 3.5$\ell$/10a with seeding at the drilling and broadcasting.

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Screening of Insecticides for Control of Spodoptera exigua in Double Cropping after Early Rice Alisma plantago

  • Kwon, Byung-Sun;Shin, Jong-Sup;Park, Hee-Jin
    • 한국작물학회지
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    • 제46권5호
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    • pp.345-347
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    • 2001
  • This study was conducted to evaluate the effect of insecticides on control of Spodoptera exigua, growth characteristics, and dry root yield in the cultivation of Alisma plantago after early maturing rice cropping. All pesticides treated had no effect on growth and flowering rate of Alisma plantago. The major insecticides were teflubenzuron Wp, 5%, tebufenozide Wp, 8%, iufenuron Ec, 5%, and tebufenozide Wp, 5%. Dry root yield were increased largely with tebufenozide Wp, 5%(20 g/20 l), insecticide than the other insecticides and no control. All insecticides had no injury with standard dosage. On the other hand, all insecticides had slight injury in the double dosage level for the Alisma plantago.

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Chemical Control of Brown Leaf Blight in Alisma plantago Double Cropping after Early Rice

  • Shin, Jong-Sup;Kwon, Byung-Sun;Park, Hee-Jin
    • 한국작물학회지
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    • 제46권5호
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    • pp.348-351
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    • 2001
  • This study was conducted to evaluate the control effect of fungicides on control of brown leaf blight, growth characteristics, and dry root yield in the cultivation of Alisma plantago after early maturing rice cropping. All fungicides treated had no effect on the growth and flowering rate of Alisma plantago. The major fungicides were mancozeb Wp, 75%, chlorothalonil Wp, 75%, dithianon Wp, 43%, difenoconazole Wp, 10%, benomyl Wp, 50%, and propineb Wp, 70%. Dry root of yield were increased largely with chlorothalonil Wp, 75%(33 g/20), fungicide than the other fungicides and control. All fungicides had no injury with standard dosage. On the other hand all fungicides had slight injury in the double dosage level for the Alisma plantago.

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Screening of Insecticide to Control Rhophalosiphum nymphaeae for Alisma plantago as Second Crop in Paddy Field

  • Kwon, Byung-Sun;Lim, June-Taeg;Shin, Jeong-Sik
    • 한국작물학회지
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    • 제48권1호
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    • pp.17-19
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    • 2003
  • The purpose of this study was to evaluate the effect of pesticides on insect pest control of Rhophalosiphum nymphaeae, growth characteristies and dry root yield from the cultivated after early maturing rice cropping. All pesticides treated had no effect on the growh period, flowering rate of Alisma plantago. The major pesticides were methomyl-Lf, 24.1 %, imidacloprid-Wp, 10%, carbosulfan-Wp, 20%, and methomyl-Wp, 45%. Dry yield of root were increased largely with imidacloprid-Wp, 10% (10g/20$\ell$), pesticide than the other pesticides and control. All pesticides were had no injury with recommended concentration. On the other hand all pesticides were slightly harmful in the double dosage level for the Alisma plantago.

강섬유보강 초고강도 시멘트 모르터의 수축저감에 관한 연구 (A Study on the Shrinkage Reducing Properties of Steel Fiber Reinforced Ultra High Strength Cement Mortar)

  • 한동엽;허영선;배장춘;한천구
    • 한국건축시공학회:학술대회논문집
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    • 한국건축시공학회 2005년도 추계 학술논문 발표대회
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    • pp.67-70
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    • 2005
  • Ultra high strength concrete, nowadays, has been widely applied to construction of high-rise building. To improve ductility and mechanical properties, steel fiber is employed in UHSC. This study investigates practical application of expansive additives(EA) and shrinkage reducing agent(SRA), in order to secure volumetric stability and improved mechanical properties, such as autogenous shrinkage and dry shrinkage of steel-fiber-reinforced-ultra-high-strength-comet-mortar(FHSM). According to the test, individual addition of steel fiber does not affect shrinkage reduction, as expected. However FHSM, with combined addition of 5$\%$ of EA and 1$\%$ of SRA decreased 60$\%$ of autogenous shrinkage. It is considered that Proper combination of EA and SRA can secure the shrinkage resistance of FHSM.

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