Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Expression of Thermostable $\alpha$-Glucosidase from Thermus caldophilus GK24 in Recombinant Saccharomyces cerevisiae

  • Choi, Jae-Youl (EnzBank, Inc., 309 Bioventure Center (BVC), Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Ahn, Jung-Oh (Bio-pilot Plant, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Sun-Il (Department of Chemical and Biochemical Engineering, Chosun University) ;
  • Shin, Hyun-Jae (Department of Chemical and Biochemical Engineering, Chosun University)
  • Published : 2006.12.30
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Abstract

A gene (GenBank AF096282) coding for a $\alpha$-glucosidase (TcaAG, EC 3.2.1.20) from Thermus caldophilus GK24 was expressed in Saccharomyces cerevisiae, a generally recognized as safe (GRAS) host. The thermostable $\alpha$-glucosidase was produced inside of the GRAS host at 0.04 unit/mg-dry cell by the constitutively expressing ADH1 promoter and at 1.2 unit/mg-dry cell by the inductively expressing GALl0 promoter, respectively. No $\alpha$-glucosidase activities were found in the medium when the MF-alpha signal sequence from S. cerevisiae or $\alpha$-amylase signal sequence from Aspergillus oryzae were fused before the $\alpha$-glucosidase gene for the secretion.

Keywords

References

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