Staphylococcus aureus, a major human pathogen, produces a wide array of toxins, which causes various types of disease symptoms. Prevalence of S. aureus in various foods collected during 2006-2008 in Korea was investigated. S. aureus was isolated from 275 of 5,186 (5.3%) food samples collected from hyper-markets in Korea. Seasonal temperature affected the prevalence of S. aureus in various foods with high isolation rate during the summer. Most of the enterotoxigenic strains produced enterotoxin A only or enterotoxin A in combination with another toxin. A total of 54.5% of the tested strains contained either one or more enterotoxin genes and 3.6% possessed a tst gene. This study offers basic information for securing the stability of food during storage and circulation, and provides an epidemiological tool to study the cause, origin and temporal spread of S. aureus food poisoning.
Kim, Jonggun;Park, Youn Shik;Lee, Seoro;Shin, Yongchul;Lim, Kyoung Jae;Kim, Ki-sung
Journal of The Korean Society of Agricultural Engineers
/
v.59
no.4
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pp.97-107
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2017
This study is to determine the coefficients of regression equations and to select the optimal regression equation in the LOADEST model after classifying the whole study period into 5 flow conditions for 16 watersheds located in the Nakdonggang waterbody. The optimized coefficients of regression equations were derived using the gradient descent method as a learning method in Tensorflow which is the engine of machine-learning method. In South Korea, the variability of streamflow is relatively high, and rainfall is concentrated in summer that can significantly affect the characteristic analysis of pollutant loads. Thus, unlike the previous application of the LOADEST model (adjusting whole study period), the study period was classified into 5 flow conditions to estimate the optimized coefficients and regression equations in the LOADEST model. As shown in the results, the equation #9 which has 7 coefficients related to flow and seasonal characteristics was selected for each flow condition in the study watersheds. When compared the simulated load (SS) to observed load, the simulation showed a similar pattern to the observation for the high flow condition due to the flow parameters related to precipitation directly. On the other hand, although the simulated load showed a similar pattern to observation in several watersheds, most of study watersheds showed large differences for the low flow conditions. This is because the pollutant load during low flow conditions might be significantly affected by baseflow or point-source pollutant load. Thus, based on the results of this study, it can be found that to estimate the continuous pollutant load properly the regression equations need to be determined with proper coefficients based on various flow conditions in watersheds. Furthermore, the machine-learning method can be useful to estimate the coefficients of regression equations in the LOADEST model.
Enteroviruses were identified and characterized from patients with aseptic meningitis and other enterovirus-related diseases in Chungnam, Korea from 2005 to 2006. Enteroviruses were isolated from 79 of 519 cases (15.2%) in 2005, and 37 of 386 cases (9.6%) in 2006. Based on partial VP1 sequencing, a total of 116 enterovirus isolates were resolved into 13 types. Prevalent among the Chungnam isolates were echovirus 18 and coxsackievirus B5 in 2005, and echoviruses 5 and 25 in 2006. This is the first time echoviruses 5 and 18 have been identified in Korea since enterovirus surveillance began there in 1993. The temporal distribution of enterovirus epidemics in Chungnam showed a remarkable seasonal pattern, with cases occurring during most of the three months of the summer from June to August. The highest rate of enterovirus-positive cases occurred in patients less than 1 year of age. The ratio of male to female enterovirus-positive patients was approximately 1.8:1. Comparison of the VP1 amino acid sequences of the 15 coxsackievirus B5 isolates with reference strains revealed that all Chungnam isolates are substituted at positions 23 (V231), 19 (S19G), 75 (Y75F), and 95 (N95S). Upon comparing the nine ECV5 isolates with foreign strains, it was found that only the Chungnam isolates, with the exception of Kor06-ECV5-239cn, have P at position 153 and F at position 146. The three ECV9 isolates from 2006 show alterations at amino acids 36, 148, and 154 outside of the BC-loop and at position 84 in the BC-loop, whereas the seven isolates from 2005 and the other ECV9 strains in the database only show the alteration at position 84 (D, I, N, S). The five ECV25 isolates have an S residue at position 134, whereas most of the foreign strains have an N residue.
Park Kwan-Ha;Kim Ju-Wan;Park Eum-Mi;Lim Chul-Won;Choi Min-Soon;Choe Sun-Nam;Hwang In-Young;Kim Jung-Sang
Environmental Analysis Health and Toxicology
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v.21
no.2
s.53
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pp.103-113
/
2006
This study examined effects of crude oil on the phase II drug-metabolizing enzymes UDP-glucuronosyl transferase (UDPGT) and glutathione S-transferase (GST) in mussel Mytilus edulis and rockfish Sebastes schlegeli, a representative bivalve and a culture fish, respectively. This work also intended indirectly to evaluate the post impact recovery from the massive oil tanker spillage accidents occurred during the summer of 1995 in the sea area off Yosu City, Chonnam. For these, enzyme activities of UDPGT and GST were examined in the fish and mussel following laboratory exposure to fresh crude oil, weathered oil, field-obtained oil residues, or in the field biota samples. Decreased GST activity was observed in rock fish following exposure to oil-soluble fraction (OSF) of fresh oil. A similar diminished GST activity was also observed after OSF of artificially weathered oil. OSF of field oil residues retrieved from the spillage area approximately 1 year later also exerted a slight inhibition of GST to rockfish. There was neither a change in UDPGT in rockfish, nor were there changes in mussel in both enzymes to any oil fractions. We could not observe any difference in the two enzymes either in rockfish or mussel sampled from the field during $1.5{\sim}2.0$ years post spillage, indicating that their enzyme systems might had been recovered by the sampling time. In conclusion, it seems that the inhibition of GST activity in rockfish is a biomarker response to crude oil exposure. The results, however, must be interpreted with care, as the inhibition nay reflect various factors such as oil concentration, duration and water temperature.
Journal of the Korea Academia-Industrial cooperation Society
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v.17
no.11
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pp.443-452
/
2016
Six preservice teachers who participated in the "2016 service of summer vacation daycare programs by university and college students" focused on the programs for the formation of art, music and physical education and recreation culture were selected as research participants. The meaning of the experience they had through the service process was qualitatively analyzed. The subjects accepted the purpose of the self-directed service in which they should themselves solve a series of the entire process, from planning a volunteer program through drawing up the budget and purchasing materials to practice, evaluation and expressed the intention of participation. A volunteer program was conducted including visiting a daycare program for lower graders of elementary school (1st and 2nd grades) with more than 40 class hours during vacation. As research materials, research participants' journals, transcripts of individual interviews and group interviews, transcripts of phone calls, e-mail messages, and self-evaluation records of each class hour were collected. To increase the reliability and validity of the study, triangulation, member check, advice and review of the experts were conducted. In the study results, the volunteering experience-related significance for the preservice teachers who participated in the self-directed service were broadly categorized into 'Becoming a capable professional teacher', that consists of 'Increasing the power of thinking', 'Realizing the importance of communication', 'Doing together itself is important' and 'Ability to apply information and resources is needed'. 'Increase the continuity between preschool and elementary education' was subdivided into 'Get to know the necessity of the continuity between preschool and elementary education' and 'Want to learn the continuity between preschool and elementary education.'
This study was conducted to estimation of change characteristics for water quality by the dyke gate operation in the Keum River estuary. The estimation data made use of surveyed data in Keum River estuary by NERDI (National Fisheries Research and Development Institute) during $1990\~1999$. Shown to compare water quality changes at st. A and st. D in Figure 1, the concentrations of TSS, COD and nutrients at st. A were as high as about $2\~4$ times than those at st. D due to affection of fresh water discharge in the Keum River. The percentages of water quality change at surface water by dyke gate operation in the Keum River estuary were shown that TSS (Total Suspended Solid) was decrease to $56\%,\;47\%$ at st. A and D, and COD (Chemical Oxygen Demand) was increase to $68\%,\;71\%$ at st. A and D, respectively. The changes percentage of DIN (Dissolved Inorganic Nitrogen) by dyke gate operation in the Keum River estuary were increase high to $95\%$ at surface water and $7\sim30\%$ at bottom water, but those of DIP (Dissolved Inorganic Phosphorus) were increase to $2.8\sim8.6\%$ at surface water and $28\%$ at bottom water. The range of fluctuation for water quality at each station by dyke gate operation has shown that salinity and TSS are little better than before dyke gate operation, but COD show highly fluctuation. Also we studied estimation of characteristics of water quality change by the season, COD was increased except the summer, TSS was decreased to all season. DIN was increased to about $61\sim172.1\%$ for all season, but DIP was increased to the spring and decreased to the autumn, DIN enrichment in the estuary by dyke gate operation are interpreted to improvement of organic matter decomposition and nitrification by increasing the residence time and to increase nutrient flux in sediments due to decreasing dissolved oxygen and increasing a deposit matter.
The seasonal characteristics of atmospheric particulate matter (PM) were evaluated through the measurement of $PM_{10}$ (particles with an aerodynamic diameter of less than 10 ${\mu}m$) and $PM_{2.5}$ (particles with an aerodynamic diameter of less than 2.5 ${\mu}m$) collected in the downtown area of Iksan city over roughly two weeks in each season of 2004. During the sampling period, 54 samples of $PM_{10}$ and $PM_{2.5}$ were collected and then measured for mass concentrations of PM and its water-soluble inorganic ion species. The concentrations of $PM_{10}$ and $PM_{2.5}$ were highly variable on a daily time scale in all seasons, especially in fall. Annual concentrations of $PM_{10}$ and $PM_{2.5}$ were $54.7{\pm}21.6\;{\mu}g/m^3$ and $34.0{\pm}13.4\;{\mu}g/m^3$, respectively. The daily concentrations of the analyzed ions similarly showed a pronounced variation, although a difference between seasons existed. Among them, $SO_4^{2-}$, $NO_3^-$ and $NH_4^+$ were the most abundant ions in all seasons, contributing up to 32% of $PM_{10}$ and 39% of $PM_{2.5}$. The contribution of $SO_4^{2-}$ and $NO_3^-$ showed a seasonal variation, as $SO_4^{2-}$ was the highest during spring and summer and $NO_3^-$ was the highest during fall and winter. Non-seasalt $SO_4^{2-}$ and $NO_3^-$ were found to exist mainly as neutralized chemical components of $(NH_4)_2SO_4$ and $NH_4NO_3$ due to the high concentration of $NH_4^+$ in PM samples, which were a major form of airborne PM in all seasons. Seasonal characteristics of $PM_{10}$ and $PM_{2.5}$ in Iksan were described in relation to the temporal variations of daily concentration of PM and its inorganic ion species including inter-particle reactions.
Journal of The Korean Association For Science Education
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v.18
no.1
/
pp.71-82
/
1998
The main purpose of Iowa Chautauqua Program(ICP) and Iowa-Scope, Sequence. and Coordination (Iowa-SS&C) was to educate K-12 science teachers so that they could teach science in a human context using the constructivist strategies. The major task for the participating teachers to perform was to develop modules with the emphasis on STS and constructivist approach. which were supposed to be taught in the respective classrooms. Teachers worked together for two weeks for the development of modules during the summer, and attempted the modules with their students. The participating teachers met regularly during the autumn semester to share and reflect their experiences and obtain additional information on science education reform and its implications. During the winter break. the teachers improved and extended the STS modules. The Iowa Chautauqua Program and Iowa-SS&C had two important implications for Korean science education. First, they made the most of the resources they had in order to provide the practicing teachers with recent research findings on the nature of science. science learning and teaching, that is. the constructivist approach. Second, they emphasized both cooperation of teachers and long-term training to realize the reform in school science education.
Journal of agricultural medicine and community health
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v.9
no.1
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pp.46-55
/
1984
The socio-psychiatric study and personality analysis by Minnesota Multiphasic Personality Inventory on attempted suicides with drug intoxication in Seonsan-Gun and Cheongdo-Gun of Kyoungsang buk-Do was attempted to identify overall picture of attempted suicides and to detect some characteristics of personality. The total of 93 suicide attempted cases which were brought to the local clinics for the past 3 years from February, 1981 to February,1984 were sampled among them. The socio-psychiatric study and MMPI were performed with 45 attempters possible to be interviewed. The personalities of a group which answered "Still have the desire occasionally" or "Don't know about the present attitude toward suicide was compared with those of the other group who answered "Will not do it agagin". The results were as follows; 1) The sex ratio of the total attempters (93 cases) were 138.5. The highest rate by age group was found in the 3rd decade to be 27 cases. On the other hand, the sex ratio of interviewed cases were 136.8 and the highest rate was found in the age group of 50-59. 2) For the common characteristics of majority other than sex, 55.7% of cases were graduated primary school, and 62.2% were engaged in agriculture. 3) Except the fact that more of the attempted suicides happened during summer, there were no seasonal variation among other seasons. 4) The places selected for the attempted suicides were the houses in majority of cases (98%). Most of cases (53.3%) were found immediately after the attempt and 24.4% within one hour. For the poison taken, 86.7 took a sort of herbicides or insecticides, 55.6% were admitted to local clinics for only one day and 20% for two days. 5) The most common precipitating factors of the attempted suicides were family trouble and financial poverty. 6) The psychodynamic motives of the attempters were impulsive hostility(35.6%), abandonment(33.3%), guilt feeling (11.1%), manipulation and attention seeking (8.9%) and others. 7) In regard to their present attitude toward suicide, 29 cases (64.4%) expressed that they would never do such an act again, 37.8% of cases expressed that they really wanted to die at the time when they attempted. 8) The Psychopathic deviate, Paranoia, Depression, Schizophrenia scores were significantly higher in the attempters group who answered "Don't know" or "Possibly" for the question of "Will you attempt suicide again?" Than the group who answered" will not do it again".
This research was carried out to study the seed dormancy of radishes which were used for summer vegetation. The differences of dormancy of cultivars, maternal effects, the effect of full day length to mother plants, and the effects of chemical treatments for breaking the seed dormancy were considered for this study. These results sere summarized as follows; 1. The germination percentage of 7 cultlvars sown just after harvest were as follows;SP70-:0, PS11-:37.5, GJ75-:75.5, JD127-:77.5, YH85-:81.0, M55-:96.5, and JC73-:98.0%. 2. The dormany of $F_1$ seed of radish was severe when SP70-was used for mother plant. 3. Germination behavior was different by the maturity of plants grown for seed production. The dormancy of seed harvested from immature field-grown plants sown Nov. 10 was deeper than mature plants sown Mar. 22. 4. The germination ratio of tire seed of SP70-radish was increased by treatment of full day length to tire mother plant during full period of growing or since first flowering stage, while that was 0% by treatment since yellow ripening stage. 5. GA 200 ppm+thiourea 0.2% treatment was most effective for breaking of radish seed dormancy.
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