• 제목/요약/키워드: summary measure

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Relationships among Organizational Commitment, Job Satisfaction, and Learning Organization Culture in One Korean Private Organization

  • LIM, Taejo
    • Educational Technology International
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    • 제8권1호
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    • pp.17-39
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    • 2007
  • The purpose of this study was to identify the relationships among organizational commitment, job satisfaction, and learning organization culture. This study was conducted in five sub-organizations of one Korean conglomerate company. One thousand employees were randomly and proportionately selected, with 669 useable cases obtained, for a response rate of 67%. The organizational commitment instrument used from the "affective, continuance, and normative commitment" scale (ACNCS) of Allen and Meyer (1990). The "Dimensions of Learning Organization Questionnaire" of Watkins and Marsick (1997) was used to measure learning organization culture. The short form MSQ (Minnesota Satisfaction Questionnaire), developed by Weiss, Dawis, England, and Lofquist (1967), was the third instrument used. Descriptive statistics, correlational statistics, and inferential statistics (ANOVA and t-tests) were used. Organizational commitment (except for continuance) is moderately and positively related to job satisfaction and moderately and positively related to learning organization culture. In addition, learning organization culture is weakly to moderately and positively related to job satisfaction. No differences by age were found. Four-year college graduates are more likely to have higher creating continuous learning opportunities in learning organization culture than graduate school degree holders. Males are likely to have higher affective and continuance organizational commitment than females. Employees working in R&D, Engineering, and Manufacturing (REM) are likely to have higher continuance organizational commitment than do other types of jobs. Employees are more likely to have higher learning organization culture and job satisfaction than assistant managers. Assistant managers have higher continuance organizational commitment than managers. Managers generally have higher organizational commitment, learning organization culture, job satisfaction than assistant managers. They also have higher learning organization culture than employees. Employees who had worked for less than four years in their current job and organization have higher promoting inquiry and dialogue in learning organization culture than those who have worked for ten years or more. Employees in the insurance organization have higher affective organizational commitment, learning organization culture, and job satisfaction than those of the other organizations. Finally, employees of the electronic company have higher continuance organizational commitment in learning organization culture than those of other companies. In summary, this research enables CEOs and HRD and HRM practitioners to view organizational commitment, learning organization culture, and job satisfaction as important variables in exploring diverse ranges of topics related to the workplace. And then, they can diversely apply their management, interventions and practices to fit these diverse characteristics.

계획된 간호 정보가 수면량에 미치는 영향에 관한 연구 -개심술 환자를 중심으로- (The Effect of Structured Information on the Sleep Amount of Patients Undergoing Open Heart Surgery)

  • 이소우
    • 대한간호학회지
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    • 제12권2호
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    • pp.1-26
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    • 1982
  • The main purpose of this study was to test the effect of the structured information on the sleep amount of the patients undergoing open heart surgery. This study has specifically addressed to the Following two basic research questions: (1) Would the structed in formation influence in the reduction of sleep disturbance related to anxiety and Physical stress before and after the operation? and (2) that would be the effects of the structured information on the level of preoperative state anxiety, the hormonal change, and the degree of behavioral change in the patients undergoing an open heart surgery? A Quasi-experimental research was designed to answer these questions with one experimental group and one control group. Subjects in both groups were matched as closely as possible to avoid the effect of the differences inherent to the group characteristics, Baseline data were also. collected on both groups for 7 days prior to the experiment and found that subjects in both groups had comparable sleep patterns, trait anxiety, hormonal levels and behavioral level. A structured information as an experimental input was given to the subjects in the experimental group only. Data were collected and compared between the experimental group and the control group on the sleep amount of the consecutive pre and post operative days, on preoperative state anxiety level, and on hormonal and behavioral changes. To test the effectiveness of the structured information, two main hypotheses and three sub-hypotheses were formulated as follows; Main hypothesis 1: Experimental group which received structured information will have more sleep amount than control group without structured information in the night before the open heart surgery. Main hypothesis 2: Experimental group with structured information will have more sleep, amount than control group without structured information during the week following the open heart surgery Sub-hypothesis 1: Experimental group with structured information will be lower in the level of State anxiety than control group without structured information in the night before the open heart surgery. Sub-hypothesis 2 : Experimental group with structured information will have lower hormonal level than control group without stuctured information on the 5th day after the open heart surgery Sub-hypothesis 3: Experimental group with structured information will be lower in the behavioral change level than control group without structured information during the week after the open heart surgery. The research was conducted in a national university hospital in Seoul, Korea. The 53 Subjects who participated in the study were systematically divided into experimental group and control group which was decided by random sampling method. Among 53 subjects, 26 were placed in the experimental group and 27 in the control group. Instruments; (1) Structed information: Structured information as an independent variable was constructed by the researcher on the basis of Roy's adaptation model consisting of physiologic needs, self-concept, role function and interdependence needs as related to the sleep and of operational procedures. (2) Sleep amount measure: Sleep amount as main dependent variable was measured by trained nurses through observation on the basis of the established criteria, such as closed or open eyes, regular or irregular respiration, body movement, posture, responses to the light and question, facial expressions and self report after sleep. (3) State anxiety measure: State Anxiety as a sub-dependent variable was measured by Spi-elberger's STAI Anxiety scale, (4) Hormornal change measure: Hormone as a sub-dependent variable was measured by the cortisol level in plasma. (5) Behavior change measure: Behavior as a sub-dependent variable was measured by the Behavior and Mood Rating Scale by Wyatt. The data were collected over a period of four months, from June to October 1981, after the pretest period of two months. For the analysis of the data and test for the hypotheses, the t-test with mean differences and analysis of covariance was used. The result of the test for instruments show as follows: (1) STAI measurement for trait and state anxiety as analyzed by Cronbachs alpha coefficient analysis for item analysis and reliability showed the reliability level at r= .90 r= .91 respectively. (2) Behavior and Mood Rating Scale measurement was analyzed by means of Principal Component Analysis technique. Seven factors retained were anger, anxiety, hyperactivity, depression, bizarre behavior, suspicious behavior and emotional withdrawal. Cumulative percentage of each factor was 71.3%. The result of the test for hypotheses show as follows; (1) Main hypothesis, was not supported. The experimental group has 282 minutes of sleep as compared to the 255 minutes of sleep by the control group. Thus the sleep amount was higher in experimental group than in control group, however, the difference was not statistically significant at .05 level. (2) Main hypothesis 2 was not supported. The mean sleep amount of the experimental group and control group were 297 minutes and 278 minutes respectively Therefore, the experimental group had more sleep amount as compared to the control group, however, the difference was not statistically significant at .05 level. Thus, the main hypothesis 2 was not supported. (3) Sub-hypothesis 1 was not supported. The mean state anxiety of the experimental group and control group were 42.3, 43.9 in scores. Thus, the experimental group had slightly lower state anxiety level than control group, howe-ver, the difference was not statistically significant at .05 level. (4) Sub-hypothesis 2 was not supported. . The mean hormonal level of the experimental group and control group were 338 ㎍ and 440 ㎍ respectively. Thus, the experimental group showed decreased hormonal level than the control group, however, the difference was not statistically significant at .05 level. (5) Sub-hypothesis 3 was supported. The mean behavioral level of the experimental group and control group were 29.60 and 32.00 respectively in score. Thus, the experimental group showed lower behavioral change level than the control group. The difference was statistically significant at .05 level. In summary, the structured information did not influence the sleep amount, state anxiety or hormonal level of the subjects undergoing an open heart surgery at a statistically significant level, however, it showed a definite trends in their relationships, not least to mention its significant effect shown on behavioral change level. It can further be speculated that a great degree of individual differences in the variables such as sleep amount, state anxiety and fluctuation in hormonal level may partly be responsible for the statistical insensitivity to the experimentation.

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가전제품 소비자의 Channel Equity에 관한 탐색적 연구 (An Exploratory Study on Channel Equity of Electronic Goods)

  • 서용구;이은경
    • 마케팅과학연구
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    • 제18권3호
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    • pp.1-25
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    • 2008
  • 본 연구는 가전제품 소매채널에 관한 소비자의 선호 및 이용행태를 조사하고 가전제품 구매 채널 현황과 소비자들의 점포선택과 만족도를 분석하여 소비자가 특정 채널에 대하여 가지고 있는 소위 channel equity에 대하여 탐색적으로 접근 하고자 한다. 분석결과 가전제품 멀티채널 쇼핑환경은 소비자로 하여금 채널별로 차별화된 구매 패턴과 쇼핑동기를 만들어 주고 있었다. 백화점이나 대리점은 품질의 우수성과 A/S 측면에서 우세하며 대형할인점, 양판점, TV홈쇼핑, 인터넷쇼핑몰, 전자제품 판매 상가는 가격적인 측면이 경쟁 우위로 조사되었다. 채널별 소비자 만족도에 있어서는 애프터서비스가 잘되고 있는 백화점이나 대리점 등이 만족도가 상대적으로 높은 소매 채널임을 알 수 있다. 채널 에퀴티의 구성요인은 가격 경쟁력과 비교구매, 이용편리성, A/S, 판매원의 전문성, 배송의 신속성, 제품 검색용이, 판매원의 친절성, 매장의 쾌적성, 교통 편리성 등을 들 수 있다. 백화점의 경우 거의 모든 요소에서 가장 높은 만족도를 가지고 있어 채널 에퀴티가 높게 평가되었다. 인터넷 쇼핑몰은 제품을 쉽게 검색할 수 있다는 점에서 우위에 있으며 TV홈쇼핑의 경우는 비교구매와 가격경쟁력에서 우위가 있음을 알 수 있었고 채널별로 채널 에퀴티를 구성하는 요인들의 상대적 비중은 매우 달랐다. 본 연구에서는 채널 만족도를 평가한 후 에퀴티 포트폴리오와 채널별 에퀴티 구성 결과를 제시하고 있으나 향후 채널 에퀴티의 개념과 관리 툴에 대한 본격적인 연구가 요망된다.

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사례기반 추론기법과 인공신경망을 이용한 서비스 수요예측 프레임워크 (A Hybrid Forecasting Framework based on Case-based Reasoning and Artificial Neural Network)

  • 황유섭
    • 지능정보연구
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    • 제18권4호
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    • pp.43-57
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    • 2012
  • 제조업에 있어서 판매 후 서비스 건수와 내용 등은 향후 서비스 제공을 위한 자원배분의 효율성 증진과 서비스 품질 향상을 위해서도 매우 중요한 정보이다. 따라서 기업들은 향후 발생하는 판매 후 서비스에 대해 정확히 예측하고 그에 따라 적절히 대처하는 능력을 확보할 필요성이 제조업을 중심으로 증가하고 있다. 그러나 실제로 이들 기업들이 활용하고 있는 서비스 수요예측 방법들은 전통적인 통계적인 예측기법이거나, 시뮬레이션을 기반한 기법들이다. 예를 들면, 전통적인 통계적인 예측기법으로는 회귀분석(regression analysis)의 경우, 다양한 제품모델에 대한 판매 후 서비스 발생 패턴이 선형적인 관계가 매우 적음에도 불구하고 선형으로 가정하여 추정한다는 점과 적정한 회귀식을 가정하여야 되며, 이러한 가정이 실제 경영환경에서는 매우 어렵다는 점 등이 기존의 예측기법들의 한계점으로 지적되고 있다. 본 연구에서는 디지털 TV 모델을 생산 판매 하는 A사의 사례연구를 통하여 최근 인공지능연구에서 각광을 받고 있는 사례기반추론(case-based reasoning; CBR) 기법을 활용한 서비스 수요예측 프레임워크를 제안하고자 한다. 또한, 사례기반추론에서 핵심적인 역할 중 하나인 유사 사례추출 방법에 있어서 가장 일반적인 nearest-neighbor 방법 이외의 유사 사례추출 방법을 제안하고자 한다. 특히, 본 연구에서 제안하는 유사 사례추출 방법은 인공신경망(artificial neural network)을 활용한 자기조직화지도(Self-Organizing Maps : SOM) 군집화 기법을 활용한 유사 사례추출 방식으로 이를 활용한 서비스 수요예측 프레임워크에 구현하고, 실제 기업의 판매 후 서비스 데이터를 활용하여 본 연구에서 제안하는 서비스 수요 예측 프레임워크의 유효성을 실증적으로 검증하고자 한다.

해석수준과 메시지 프레이밍에 따른 자율주행택시의 사용의도에 관한 연구 (A Study on the Construal Level and Intention of Autonomous Driving Taxi According to Message Framing)

  • 윤승정;김민용
    • 지능정보연구
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    • 제24권3호
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    • pp.135-155
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    • 2018
  • 본 연구는 최근 4차산업 혁명의 산물로 대두되고 있는 자율주행차가 대중교통 수단인 택시로 이용될 때 해석수준과 메시지 프레이밍에 따른 사용 의도의 차이를 분석하는 것이다. 해석수준이란 가까운 미래에 발생한 일과 먼 미래에 발생할 일을 가정하여 어떤 제품이나 서비스를 해석하는 것이 다르다는 것을 말한다. 메시지 프레이밍이란 긍정 혹은 부정의 표현 또는 혜택, 손실의 양극단의 메시지를 구성한 것을 말한다. 즉, 기존연구에서는 이 두 개념에 따라 제품이나 서비스의 가치를 다르게 해석한다고 한다. 본 연구는 자율주행차가 택시로 출시될 때 두 개념을 적용할 경우 사용 의도의 차이를 보이는지 살펴보고자 한다. 결과를 요약하면 우선, 메시지 프레이밍 구성에서 자율주행 택시를 이용할 경우 혜택(Gain)과 왜(Why) 사용해야 하는지를 설명한 메시지 형식과 자율주행 택시를 이용하지 않았을 경우 손실(Loss)와 어떻게(How)를 강조한 메시지를 구성하여 비교해 보았다. 두 메시지 프레이밍은 차이를 보였으며(t= 3.063) 혜택(Gain)과 왜(Why)를 설명한 메시지 형태가 더 높은 사용 의도를 보였다. 또한, 해석수준에 따른 결과를 요약하면 다음과 같다. 혜택(Gain) 및 손실(Loss)에 대한 먼 미래와 가까운 미래에 발생할 것을 가정한 경우 사용 의도에 차이가 있었으며, 구체적으로 혜택(Gain)을 설명한 메시지와 먼 미래에 발생할 것을 가정한 경우가 사용 의도가 높았다. 요약하면 자율주행 택시의 사용 의도를 높이기 위해서는 긍정의 메시지(Gain)와 먼 미래에 일어날 수 있는 것을 가정하여 사람들에게 메시지를 전달해야 한다는 결론이다. 또한, 본 연구를 통하여 향후 신기술의 출현에 대한 사용 의도 연구 시 연구방법을 활용할 수 있을 것이다.

해외진출 국내 프랜차이즈기업의 조직특성 (Organizational Factors Facilitating the Internationalization of Korean Franchising Companies)

  • 임영균;이동휘;김희정
    • 마케팅과학연구
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    • 제19권2호
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    • pp.40-52
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    • 2009
  • Franchising is one of the fastest growing types of business. It is already popular and well-known in the U.S., and has been growing in many other countries including Korea. Furthermore, many Korean franchising companies have expanded their business overseas actively. According to the data by the Ministry of Industry and Resource, 82 companies out of a sample of 500 franchising companies are already operating in many foreign countries and 48% of them have started their foreign business since 2006. This clearly indicates the fast growing current trend of foreign operation by Korean franchising companies. In spite of the fast growing trend of foreign expansion in the industry, academic research on internationalization of franchising companies is extremely difficult to find. Accordingly, academic research on the issue is necessary and urgent in Korea. Among the various research questions on internationalization of franchising business, this study intends to investigate the difference in organizational factors between the franchising companies doing foreign operation and those doing business only domestically. More specifically, this research has the following purposes. First, considering the lack of theoretical basis of previous studies, resource-based theory and agency theory are employed as the theoretical bases. Second, this study explains the difference in internationalization based on organizational factors such as company size, history and growth rate. Third, the five hypotheses regarding the difference in organizational factors are presented and tested empirically, which is the first attempt in the area of this topic. Finally, the study attempts to clarify the conflicting implications among theories regarding some organizational factos such as growth rate. As the theoretical background, resource-based theory and agency theory are discussed. According to resource-based theory, a firm can grow continuously when it has competence and resource, and also the ability to develop them. The competence and resource can include capital, human resource, management skill, market information, ability to manage risk, etc. Meanwhile, agency theory views the relationship between franchisor and franchisee as an agency relationship. In agency theory, bonding capability and monitoring capability are the two key factors which promote internationalization of franchising companies. Based on the two theories, a conceptual model is designed. The model consists of two groups of variables. One is organizational factors including size, history, growth rate, price bonding and geographic dispersion. The other is whether a franchising company is operating overseas or not. We developed the following five research hypotheses basically describing the relationship between organizational factors and internationalization of franchising companies. H1: The size of franchising companies operating overseas is larger than that of franchising companies operating domestically. H2: The history of franchising companies operating overseas is longer than that of franchising companies operating domestically. H3: The growth rate of franchising companies operating overseas is higher than that of franchising companies operating domestically. H4: The price bonding of franchising companies operating overseas is higher than that of franchising companies operating domestically. H5: The geographic dispersion of franchising companies operating overseas is wider than that of franchising companies operating domestically. Data for the analyses are obtained from 2005 Korea Franchise Survey data co-generated by Ministry of Industry and Resource, GS1 Korea, and Korea Franchise Association. Out of 2,804 population companies, 2,489 companies are excluded for various reasons and 315 companies are selected as the final sample. Prior to hypotheses tests, validity and reliability of the measures of size, history, growth rate and price bonding are examined for further analyses. Geographic dispersion is not validated since it is measured using nominal data. A series of independent sample T-tests is used to find out whether there exists any significant difference between the companies internationalized and those operating only domestically for each organizational factor. Among the five factors, size and geographic dispersion show significant difference, growth rate and price bonding do not reveal any difference and, finally, history factor shows conflicting results in the difference depending on how to measure it.

    shows the summary statistics for hypotheses testing. In conclusion, the results show that the size and history, which are the key variables in resource-based theory, have a significant relationship with internationalization and that geographic area, which belongs to agency theory, also has a strong relationship with internationalization. The results support the findings of extant research and, therefore, prove the usefulness of resource-based theory and agency theory in explaining internationalization of franchising companies. However, growth rate and price-bonding do not show a clear difference between the two types of companies. Accordingly, these two factors need further attention in the future research. Although this study shows meaningful findings theoretically and practically, it has several limitations. First, only organizational factors are considered even if there are various environmental factors influencing franchising firm's internationalization. Second, only being internationalized or not is considered. That is, modes of entry and the size of foreign operations are not included in the study. Third, internationalization strategy is often determined based on the desire for business expansion and higher profitability and egoistical reasons of the CEOs. However, this type of factors belonging to behavioral science is not discussed in the study. Finally, organizational ecology perspective is usefully applicable in explaining the survival and performance of internationally operating companies. Accordingly, research propositions based on this perspective need to be developed and tested.

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  • 간호사의 복장 형태에 따른 간호사 이미지에 대한 조사 연구 (A Survey of the Image Conveyed by Different Types of Nurses따 Uniforms)

    • 김조자;이원희;허혜경;김창희;홍성경
      • 대한간호학회지
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      • 제23권4호
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      • pp.631-648
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      • 1993
    • This study is a descriptive study to provide basic data related to types of uniforms worn by nurses. Nurses, other medical center personnel, patients and their famillies were asked for their opinion on changing nurses’ uniforms and how the image of nurses is 1 elated to the type of uniform worn by the nurses. The data for this study were collected during the period from Feb. 25, 1993 to Mar. 26, 1993 at Y-University Medical Center from 132 nurses, 137 other medical center personnel, 117 patients and their families. The instruments used for this study were the Nurses’ Image Scale(NIS) developed by Bown(1986), and an instrument to measure opinions of uniform and cap using photographs of nurses’ uniforms that was developed by the researcher through a literture review. Data were analyzed using the SPPS / PC package, Statistics used for analysis were frequencies, percentages, paired t -test, and oneway ANOVA The results of this study are summarized as follows ; 1. Of the nurses, 84.3%, along with, 51.5% of the medical center personnel, 39.1% of the patients and their families agreed to a change in the color of nurses uniforms. 2. Similarly, 87.4% of the nurses,27.7% of the medical center personnel, 19.3% of the patients and their families were in favor of nurses not wearing caps. 3. Only 11.8% of nurses answered that a white uniform was important to the professional image of nursing. Only 2.4% of the nurses answered that a cap was very important to the professional image of nursing. 4. Only 1.6% of the nurses answered that a white uniform and cap were important to show the role of nurses in the twenty first century. 5. About 1/3, 36.5%, of the medical center personnel, the patient and their families answered that changing the color of the nurses' uniform will make a change in the image of nurses. 6. A White uniform and cap were seen as being im-portant in distinguishing nurses from other medical personnel in the hospital by 76.5% of the medical personnel, the patients and their families. 7. Nurses gave high marks to the idea of no cap regardless of the color of the uniform which would still portray the symbol of the nurses’ role, identity and would differentiate the role from other jobs. The patients and their families gave high marks to a white uniform with cap. 8. Generally, nurses, medical center personnel and patients and their families thought that a colored uniform with cap presented a good image of nurses. 9. There was a significant difference in the response among the three groups(F=24.65, P〈.001) to the figure in a white uniform with a cap, and the patients and their families thought it was the best portrayal of the image of nurses. There was a significant difference among the three groups(F=9.03, P〈.001) to the figure in a white uniform with no cap and the nurses indicated that it was the beat portrayed of the image of nurses. There was no significant difference among the three groups to the figure in a colored uniform with cap. There was a significant difference(F=17.50, P〈. 001) to the figure in a colored uniform with no cap, and the nurses indicated that it was the best portrayal of the image of nurses. In summary, the nurses wanted to change the color of uniform and not to wear a cap. But many of the medical center personnel, patients and their families indicated they did not agree with this idea. Therefore, changing the type of uniform worn by nurses should be done only after consideration has been given to the relation of the change to the professional role of nurses and the image of nurses held people in general. Suggestions arising from this study are as follows : 1. The relation between the role of nurses and their uniform was surveyed, and these results can be used as data when considering a change in uniforms. 2. A change in uniforms should be tried based on the norm established about uniforms. 3. The nurses did not want to wear a cap, but before a decision is definitely made it is necessary to study the philosophy related to the symbol of the cap because the figure of a nurse with a cap portrayed a positive image of the nurses. If the cap is kept, the symbol of the cap should be redefined. 4. In this study, only the image related to the uniform was examined, but in future studies it will be necessary to examine the practicality of various types of uniforms.

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    초기 창업기업 창업지원정책의 효과성 분석: 창업업종 및 창업성장단계 조절효과 (Effectiveness Analysis of Startup Support Policy of Early Start-ups: Moderating Effect of the Industry and Growth Stage of the Start-ups)

    • 정경희
      • 벤처창업연구
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      • 제15권1호
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      • pp.59-70
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      • 2020
    • 본 연구는 초기 창업기업 성과에 영향을 미치는 요인으로 창업지원정책을 실증적으로 규명하고, 현재의 창업지원정책에 대한 효과성을 측정하여, 이를 토대로 향후 정책적 방향을 제시하기 위한 것이다. 이를 위해 창업지원정책이 초기 창업기업의 성과에 미치는 영향에 대해 분석하고, 창업기업의 특성으로 창업업종 및 창업성장단계에 따라 어떠한 차이점이 있는지 확인하였다. 연구대상은 초기창업패키지 사업에 선정된 최근 3년간 창업기업 297개의 실제 테이터를 수집하여, 변수 간 영향력은 다중회귀분석, 조절효과는 위계적 회귀분석을 실시하였다. 연구결과를 요약하면 다음과 같다. 첫째, 창업지원정책과 창업성과의 영향 관계 확인 결과, 매출은 창업자금, 창업멘토링, 창업인프라(공간) 모두 유의한 영향을 보였으며, 반면 창업교육은 매출증가에 유의한 영향을 미치지 못하였다. 고용은 창업멘토링만이 유의한 영향 관계임을 확인하였다. 둘째, 창업업종과 창업성장단계의 조절효과 확인 결과, 창업업종은 통계적으로 유의한 영향력을 미치지 못하였으나, 상호작용 효과는 창업교육에서 유의하게 나타났다. 구체적으로 업종별 매출 관계는 지식서비스는 창업교육이 매출향상에 도움을 주며, 제조는 창업멘토링과 창업인프라(공간) 지원 여부와 관계없이 모두 매출향상에 효과적인 것으로 나타났다. 창업성장단계별 매출 관계는 통계적으로 유의한 영향력을 확인하였다. 예비단계는 통계적으로 유의하지 않으며, 창업단계는 창업멘토링 지원, 성장단계는 창업자금 지원이 효과적인 것으로 확인되었다. 반면 고용은 창업성장단계에 따라 유의한 영향력을 미치지 못하였다. 본 연구는 초기 창업기업 대상 창업지원정책의 효과성을 분석하여, 창업기업 특성에 따른 차별적 정책지원의 필요성을 확인하고, 향후 정책적 방향에 대한 시사점을 제시하였다.

    더덕 부위별, 첨가수준이 실험쥐의 항산화활성과 지질조성에 미치는 효과 (Antioxidative Activity and Lipid Composition from Different Part and Supplement of Codonopsis lanceolata in Rat)

    • 오혜숙;원향례
      • 한국식품영양과학회지
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      • 제36권9호
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      • pp.1128-1133
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      • 2007
    • 본 연구는 더덕 육질과 더덕 껍질의 항산화 효과와 지질대사를 동물실험을 통해 확인하고자 6주령 된 Sprague Dawley 종 흰쥐 40마리를 대조군, 더덕 육질 2.5% 첨가군(LF), 더덕 육질 5% 첨가군(HF), 더덕 껍질 2.5% 첨가군(LS), 더덕 껍질 5% 첨가군(HS)의 총 5군으로 나누어 실험식이로 6주간 사육 후 혈액 및 간 microsome 내의 항산화효소 활성, 지질 과산화물과 지질 조성을 측정한 결과 혈액의 glutathione peroxidase 활성은 더덕 첨가군이 모두 높았으며 첨가 수준보다는 부위에 따른 차이를 보였고 혈액 glutathione reductase의 활성과 MDA(malondialdehyde) 생성량은 더덕 부위보다는 첨가 수준에 따른 차이를 보여 주었다. 간의 glutathione peroxidase 활성은 더덕 첨가 여부에 따라 항산화 활성의 차이를 보였으며 껍질의 경우 5% 첨가군이 더 높은 활성을 나타냈다. Glutathione reductase 활성은 더덕 육질 첨가군은 모두 대조군과 차이를 보이지 않았으나 더덕 껍질 첨가군은 5% 첨가군이 대조군에 비해 높게 나타났다. MDA 생성량은 대조군에 비해 더덕 첨가군에서 더덕 부위와 첨가 수준에 관계없이 모두 낮게 나타났다. 혈액의 총 콜레스테롤과 LDL-콜레스테롤은 더덕 육질이나 껍질을 첨가한 군이 대조군보다 유의하게 낮게 나타났다. 더덕 첨가의 수준과 더덕 부위에 따른 차이는 없었다. 혈액 HDL-콜레스테롤도 더덕 첨가의 수준에 따른 차이는 볼 수 없었으나 더덕 부위에 따른 차이, 즉 껍질보다는 육질을 첨가했을 때 HDL-콜레스테롤이 더 높게 나타났다. 간의 총콜레스테롤도 더덕 육질이나 껍질을 첨가한 군이 대조군보다 유의하게 낮게 나타났다. 더덕 첨가의 수준과 더덕 부위에 따른 차이는 없었다. 간의 중성지방은 5% 더덕 육질을 첨가한 군이 대조군보다 유의하게 낮게 나타났다.이 결과를 요약해 볼 때 흰쥐에서 더덕 껍질이나 더덕 육질 첨가 식이의 항산화 효과와 지질 조성의 개선 효과를 볼 수 있었다. 따라서 더덕 육질은 물론 껍질도 식품 내 첨가물로서, 혹은 다른 약용으로의 활용 가능성을 제시하고 있다.

    Ham's F-10 배양액에 첨가된 태아제대혈청과 EDTA가 백서 수정란의 분할에 미치는 영향 (The Effect of EDTA and Fetal Cord Serum Supplementation to Ham's F-10 Culture Medium on Developmental Potential of Mouse Embryos In Vitro)

    • 김병석;이영기;박윤기;이태형;이승호
      • Journal of Yeungnam Medical Science
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      • 제12권1호
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      • pp.124-134
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      • 1995
    • Ham's F-10 기본 배양액과 이 배양액에 여러가지 농도의 EDTA와 태아제대혈청을 단독 또는 같이 혼합한 배양액을 얻어 각각의 배양액에서 5-10주된 백서를 이용하여 2 세포기 배아의 단계적 분할정도를 96시간 동안 관찰하였다. Ham's F-10 기본 배양액에 비해 태아제대혈청이나 EDTA $50{\mu}M$에서 $100{\mu}M$을 단독 또는 함께 첨가한 배양액에서 2 세포기의 배아의 상실배기 난할률이 매우 높았으며 (p<0.05), 포배기 난할률은 태아제대혈청과 EDTA $50{\mu}M$에서 $100{\mu}M$을 첨가한 배양액에서 유의하게 높았다(p<0.05). 체외수정실에서 흔히 사용되는 태아제대혈청을 첨가한 배양액과 비교시 태아제대혈청과 EDTA $100{\mu}M$을 첨가한 배양액에서 상실기 난할률이 유의하게 높았다(p<0.05). 태아제대혈청과 여러 농도의 EDTA를 첨가한 배양액과 EDTA만 첨가한 배양액의 비교에서 태아제대혈청과 EDTA $50{\mu}M$$100{\mu}M$을 첨가한 배양액에서 EDTA $200{\mu}M$만 첨가한 배양액에 비해 상실기 난할률이 매우 유의하게 높았고(p<0.05), 포배기 난할률은 태아제대혈청과 EDTA $100{\mu}M$을 첨가한 배양액이 EDTA $200{\mu}M$을 단독 또는 태아제대혈청과 함께 넣은 배양액에 비해 유의하게 높았다(p<0.05). 결론적으로 Ham's F-10 기본 배양액에 태아제대혈청과 적정 농도의 EDTA $50{\mu}M$$100{\mu}M$을 첨가한 배양액에서 초기 수정란의 분할이 우수함을 알 수 있고, 이는 수정란의 배양시 적절한 배양액을 선택할 수 있는 기초 자료로서 유용할 것으로 사료된다.

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