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A Study on the Stability of the Cream Containing Glycyrrhiza uralensis Extract (한국산 감초 추출물 함유 크림의 안정성 평가)

  • Kim, Hye Jin;Jang, Ha Na;Bae, Jeong Yun;Park, Soo Nam
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.39 no.2
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    • pp.117-125
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    • 2013
  • In this study, The stability of a cream containing the ethyl acetate fraction of 50% ethanol extracts from Glycyrrhiza uralensis (G. uralensis) cultured in Korea was investigated. pH, absorbance, viscosity and color difference of the cream containing 0.20% ethyl acetate fraction of the aforementioned G. uralensis extracts were measured under 4 different temperature conditions ($4^{\circ}C$ $25^{\circ}C$ $37^{\circ}C$ and $45^{\circ}C$) and under the sun light at 2 week intervals for 12 weeks. pH changes of a control cream without the extracts and the sample cream containing 0.20% ethyl acetate fraction of G. uralensis extracts were 0.66 and 0.44, respectively. There were no significant pH differences between the sample and the control under the sun. Viscosities of the control cream and the sample cream decreased by 2,483 cPs and 2,893 cPs respectively. So, the sample cream showed a bigger decline (410 cP) in viscosity than the control cream. The ethyl acetate fraction of G. uralensis extracts did not affect the stability of the cream. Absorbance of ethanol solution of the ethyl acetate fraction decreased 30.00% at 276 nm under the sun. On the other hand, the absorbance of the sample cream containing the ethyl acetate fraction decreased 12.02%. The stability of the G. uralensis extracts was better in cream formulation than in ethanol solution. The total color differences of all creams increased slightly during the study period under various conditions. The results appeared to indicate the color stability of the cream containing 0.20% ethyl acetate fraction of G. uralensis extracts. It is suggested that further study is needed to provide more information to the manufacturers who are seeking for the application of the G. uralensis extracts to improve the anti-oxidant and stability of cosmetic products.

Assessment of the Angstrom-Prescott Coefficients for Estimation of Solar Radiation in Korea (국내 일사량 추정을 위한 Angstrom-Prescott계수의 평가)

  • Hyun, Shinwoo;Kim, Kwang Soo
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.18 no.4
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    • pp.221-232
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    • 2016
  • Models to estimate solar radiation have been used because solar radiation is measured at a smaller number of weather stations than other variables including temperature and rainfall. For example, solar radiation has been estimated using the Angstrom-Prescott (AP) model that depends on two coefficients obtained empirically at a specific site ($AP_{Choi}$) or for a climate zone ($AP_{Frere}$). The objective of this study was to identify the coefficients of the AP model for reliable estimation of solar radiation under a wide range of spatial and temporal conditions. A global optimization was performed for a range of AP coefficients to identify the values of $AP_{max}$ that resulted in the greatest degree of agreement at each of 20 sites for a given month during 30 years. The degree of agreement was assessed using the value of Concordance Correlation Coefficient (CCC). When $AP_{Frere}$ was used to estimate solar radiation, the values of CCC were relatively high for conditions under which crop growth simulation would be performed, e.g., at rural sites during summer. The statistics for $AP_{Frere}$ were greater than those for $AP_{Choi}$ although $AP_{Frere}$ had the smaller statistics than $AP_{max}$ did. The variation of CCC values was small over a wide range of AP coefficients when those statistics were summarized by site. $AP_{Frere}$ was included in each range of AP coefficients that resulted in reasonable accuracy of solar radiation estimates by site, year, and month. These results suggested that $AP_{Frere}$ would be useful to provide estimates of solar radiation as an input to crop models in Korea. Further studies would be merited to examine feasibility of using $AP_{Frere}$ to obtain gridded estimates of solar radiation at a high spatial resolution under a complex terrain in Korea.

CCD Photometric Observations and Light Curve Synthesis of the Near-Contact Binary XZ Canis Minoris (근접촉쌍성 XZ CMi의 CCD 측광관측과 광도곡선 분석)

  • Kim, Chun-Hwey;Park, Jang-Ho;Lee, Jae-Woo;Jeong, Jang-Hae;Oh, Jun-Young
    • Journal of Astronomy and Space Sciences
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    • v.26 no.2
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    • pp.141-156
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    • 2009
  • Through the photometric observations of the near-contact binary, XZ CMi, new BV light curves were secured and seven times of minimum light were determined. An intensive period study with all published timings, including ours, confirms that the period of XZ CMi has varied in a cyclic period variation superposed on a secular period decrease over last 70 years. Assuming the cyclic change of period to occur by a light-time effect due to a third-body, the light-time orbit with a semi-amplitude of 0.0056d, a period of 29y and an eccentricity of 0.71 was calculated. The observed secular period decrease of $-5.26{\times}10^{-11}d/P$ was interpreted as a result of simultaneous occurrence of both a period decrease of $-8.20{\times}10^{-11}d/P$ by angular momentum loss (AML) due to a magnetic braking stellar wind and a period increase of $2.94{\times}10^{-11}d/P$ by a mass transfer from the less massive secondary to the primary components in the system. In this line the decreasing rate of period due to AML is about 3 times larger than the increasing one by a mass transfer in their absolute values. The latter implies a mass transfer of $\dot{M}_s=3.21{\times}10^{-8}M_{\odot}y^{-1}$ from the less massive secondary to the primary. The BV light curves with the latest Wilson-Devinney binary code were analyzed for two separate models of 8200K and 7000K as the photospheric temperature of the primary component. Both models confirm that XZ CMi is truly a near-contact binary with a less massive secondary completely filling Roche lobe and a primary inside the inner Roche lobe and there is a third-light corresponding to about 15-17% of the total system light. However, the third-light source can not be the same as the third-body suggested from the period study. At the present, however, we can not determine which one between two models is better fitted to the observations because of a negligible difference of $\sum(O-C)^2$ between them. The diversity of mass ratios, with which previous investigators were in disagreement, still remains to be one of unsolved problems in XZ CMi system. Spectroscopic observations for a radial velocity curve and high-resolution spectra as well as a high-precision photometry are needed to resolve some of remaining problems.

Simulation of Detailed Wind Flow over a Locally Heated Mountain Area Using a Computational Fluid Dynamics Model, CFD_NIMR_SNU - a fire case at Mt. Hwawang - (계산유체역학모형 CFD_NIMR_SNU를 이용한 국지적으로 가열된 산악지역의 상세 바람 흐름 모사 - 화왕산 산불 사례 -)

  • Koo, Hae-Jung;Choi, Young-Jean;Kim, Kyu-Rang;Byon, Jae-Young
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.11 no.4
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    • pp.192-205
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    • 2009
  • The unexpected wind over the Mt. Hwawang on 9 February 2009 was deadly when many spectators were watching a traditional event to burn dried grasses and the fire went out of control due to the wind. We analyzed the fatal wind based on wind flow simulations over a digitized complex terrain of the mountain with a localized heating area using a three dimensional computational fluid dynamics model, CFD_NIMR_SNU (Computational Fluid Dynamics_National Institute of Meteorological Research_Seoul National University). Three levels of fire intensity were simulated: no fire, $300^{\circ}C$ and $600^{\circ}C$ of surface temperature at the site on fire. The surface heat accelerated vertical wind speed by as much as $0.7\;m\;s^{-1}$ (for $300^{\circ}C$) and $1.1\;m\;s^{-1}$ (for $600^{\circ}C$) at the center of the fire. Turbulent kinetic energy was increased by the heat itself and by the increased mechanical force, which in turn was generated by the thermal convection. The heating together with the complex terrain and strong boundary wind induced the unexpected high wind conditions with turbulence at the mountain. The CFD_NIMR_SNU model provided valuable analysis data to understand the consequences of the fatal mountain fire. It is suggested that the place of fire was calm at the time of the fire setting due to the elevated terrain of the windward side. The suppression of wind was easily reversed when there was fire, which caused updraft of hot air by the fire and the strong boundary wind. The strong boundary wind in conjunction with the fire event caused the strong turbulence, resulting in many fire casualties. The model can be utilized in turbulence forecasting over a small area due to surface fire in conjunction with a mesoscale weather model to help fire prevention at the field.

Optimization of the cryopreserved condition for utilization of GPCR frozen cells (GPCR 냉동보관 세포의 활용을 위한 냉동조건의 최적화 연구)

  • Noh, Hyojin;Lee, Sunghou
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.2
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    • pp.1200-1206
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    • 2015
  • The major target for drug discovery, G-protein coupled receptor (GPCR) is involved in many physiological activities and related to various diseases and disorders. Among experimental techniques relating to the GPCR drug discovery process, various cell-based screening methods are influenced by cell conditions used in the overall process. Recently, the utilization of frozen cells is suggested in terms of reducing data variation and cost-effectiveness. The aim of this study is to evaluate various conditions in cell freezing such as temperature conditions and storage terms. The stable cell lines for calcium sensing receptor and urotensin receptor were established followed by storing cultured cells at $-80^{\circ}C$ up to 4 weeks. To compare with cell stored at liquid nitrogen, agonist and antagonist responses were recorded based on the luminescence detection by the calcium induced photoprotein activation. Cell signals were reduced as the storage period was increased without the changes in $EC_{50}$ and $IC_{50}$ values $EC_{50}:3.46{\pm}1.36mM$, $IC_{50}:0.49{\pm}0.15{\mu}M$). In case of cells stored in liquid nitrogen, cell responses were decreased comparing to those in live cells, however changes by storage periods and significant variations of $EC_{50}/IC_{50}$ values were not detected. The decrease of cell signals in various frozen cells may be due to the increase of cell damages. From these results, the best way for a long-term cryopreservation is the use of liquid nitrogen condition, and for the purpose of short-term storage within a month, $-80^{\circ}C$ storage condition can be possible to adopt. As a conclusion, the active implementation of frozen cells may contribute to decrease variations of experimental data during the initial cell-based screening process.

Strategy to Improve the Productivity of Broilers: Focusing on Pre-Starter Diet (초이사료 배합설계를 통한 육계 생산성 증대방안)

  • Nam, Doo Seok;Lee, Jinyoung;Kong, Changsu
    • Korean Journal of Poultry Science
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    • v.42 no.3
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    • pp.247-256
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    • 2015
  • There are approximately 1,500 broiler farms in Korea, each raising 55,000 birds. Ninety-five percent of the farms are contracted with Integration Company. According to the Korean broiler performance index, broilers in Korea are marketed at 32 days with 1.52 kg of body weight. In contrast, the market age and body weight of broilers are 47 days/2.8 kg in the United States and 42 days/2.5 kg in Europe. Because of the younger market age of the Korean broiler, the pre-starter feed is important. Chicks exhibit poor absorption of dietary nutrients up to 7 days after hatching due to an immature digestive system and low enzyme secretion rate and activity. At the beginning of hatching, chicks obtain their nutrients from the egg yolk sac. It is highly recommended that chicks, after consuming the nutrients in the egg yolk sac, are given supplemented pre-starter feed to increase early growth rates and improve the performance of broiler production. Pre-starter nutrient requirements are not expressed in NRC, so Korean feeding standards for poultry and commercial breeding companies determine the nutrient requirements of pre-starter broiler chickens. Three approaches are followed to formulate specially designed pre-starter feeds for broiler chicks: (i) selective use of raw materials, (ii) proper standards of nutrient supply, and (iii) application of feed additives such as exogenous enzymes. In the selection of raw materials, those with high digestibility can be used. The absorption rate of carbohydrates in grains can be increased through feed processing at high temperature and high pressure. Soy proteins and fish meal can also be added as protein sources. As an energy source, vegetable oils are preferred over animal fats because of the former's high digestibility. It is suggested that the levels of proteins and amino acids are higher in pre-starter feed than in starter feed. With regard to energy, the sources of energy are more important than the levels of energy in feed. Feed additives such as exogenous enzymes can be used to improve nutrient digestibility. In addition, organic acids and plant extracts can be used as alternatives to animal growth promoters to stimulate immunity and prevent diseases. The growth performance of broilers is affected by various factors, such as management and disease control, in addition to the nutritional strategy; however, nutritional strategies play an important role in improving the productivity of broilers. Therefore, nutritional strategies, along with management and disease control, are required for improving the productivity of broilers in Korea.

Effects of Pasteurization and Frozen Storage on Changes in Quality Characteristics of 10% Salted Egg Yolk (저온살균 및 냉동저장이 10% 가염난황의 품질특성에 미치는 영향)

  • Kim, Jae-Wook;Choi, Chun-Un
    • Korean Journal of Food Science and Technology
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    • v.34 no.3
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    • pp.459-465
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    • 2002
  • To obtain the basic data for commercial 10% salted egg yolk for mayonnaise preparation, 3 types of egg yolks [pasteurized egg yolk (Yolk A)-not salted, pasteurized before salting (Yolk B)-salted, and pasteurized after salting (Yolk C)-salted] were prepared, and the changes in quality characteristics of these egg yolks with frozen storage were tested. The results obtained were as follows; Yolk A gelatinized during frozen storage, thus could not used for mayonnaise preparation. The viscosity of the egg yolk increased $3{\sim}5$ times after salting. Viscosity of the salted egg yolk increased with frozen storage time. Viscosity of Yolk B was higher at $-20^{\circ}C$ than $-15^{\circ}C$. Viscosity of Yolk C, however, was higher at $-15^{\circ}C$ than $-20^{\circ}C$. Frozen storage of pasteurized salted egg yolk showed some effects on the emulsification capacity. The effect, however, was smaller than that of unpasteurized salted egg yolk. Microbes of salted egg yolk were decreased with frozen storage, but there was no difference between Yolk B and Yolk C. It was suggested that commercially pasteurized 10% salted egg yolk for mayonnaise preparation can be successfully stored for 12 months at the temperature of $-15{\sim}-20^{\circ}C$.

Preparation and Characterization of Enzymatic Oyster Hydrolysates-added Yogurt (굴 효소 가수분해물 첨가 요구르트의 제조 및 특성)

  • Chung, In-Kwon;Kim, Hye-Suk;Kang, Kyung-Tae;Choi, Jong-Duck;Heu, Min-Soo;Kim, Jin-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.7
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    • pp.926-934
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    • 2006
  • The base for preparing oyster hydrolysate-added yogurt was consisted of whole milk (1,000 mL), skim milk (44.05 to 42.05 g), enzymatic oyster hydrolysates powder (OHP, 0 to 2.0 g) and pectin. The yogurt base was fermented with 7 kinds of starter cultures (3% based on yogurt volume), such as Lactobacillus acidophilus, lactobacillus bulgaricus, lactobacillus casei, Lactobacillus fermentum, Lactobacillus pentosus, Streptcoccus thermophilus and the mixed starters (L. bulgaricus and S. thermophilus) at optimal temperature. Processing condition and quality characteristics of the yogurt were evaluated by analyzing pH, titratable acidity, viscosity, viable cell count, functional properties and sensory evaluation. The results suggested that the optimal conditions for preparing the good quality yogurt revealed the mixed starters (L. bulgaricus and S. thermophilus) for starter culture, 1.0 g of 3 kDa hydrolysate for amount, and 5.5 hrs for fermentation time. The good quality yogurt showed 4.31 for pH, 1.07% for titratable acidity, 469 cps for viscosity and $4.9{\times}10^8\;CFU/mL$ for viable cell count. The hydrolysate-added yogurt was 2 times higher in ACE inhibitory and antioxidant activities than commercial yogurt, and kept good quality during storage of 15 days at $5^{\circ}C$.

Progress of Experimental Trichophyton Verrucosum Infection in Guinea Pig (Guinea Pig에 유발시킨 Trichophyton Verrucosum 감염증의 경과)

  • Kim, Hyun-Sug;Choi, Jong-Su;Kim, Ki-Hong
    • Journal of Yeungnam Medical Science
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    • v.6 no.1
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    • pp.47-57
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    • 1989
  • T. verrucosum infection has been reported for the first time in 1986 in Korea and has been increasing progressively. To evaluate the progress of clinical and histopathological change of dermatophytosis caused by T. verrucosum, inoculation study, using T. verrucosum isolated from infected human(human strain) and from infected cattle(cattle strain), was done in 24 male albino Hartley guinea pig. Their clinical and histopathological changes were evaluated. In addition, comparison for the growth rate between human strain and cattle strain on Sabouraud's glucose agar was made. The results were as follows: 1. Growth rate on Sabouraud's glucose agar : Cattle strain showed significantly more rapid growth rate than human strain on Sabouraud's glucose rate at $25^{\circ}C$ and $37^{\circ}C$. And cattle strain showed more rapid growth rate at $37^{\circ}C$ than $25^{\circ}C$. But human strain showed no significant difference of growth rate at both temperature. 2. Clinical findings: Initial erythema, scale and crust were developed about 8th after inoculation. All three findings reached maximum severity about 12th to 16th day and disappeared about 30th to 34th day after inoculation. There was no significant difference in progress of erythema, scale and crust between cattle strain and human strain. 3. Histopathological findings: Although mild acanthosis was noticed on the 3rd day after inoculation, the other findings including parakeratosis, intraepidermal abscess, spongiosis and vascular change, cellular infiltration were found on 9th day after inoculation. They reached maximum severity on the 12th day and lasted to the 25th day after inoculation. After that, all three findings were decreased gradually between 29th day and 33th day. On the PAS stainings, hyphae and spores were found on the 6th day and disappeared on the 21th day after inoculation. 4. In trichophytin skin test, all of the 24 guinea pigs became positive within average $9.83{\pm}1.17$ lays. These findings suggested that dermatophytosis caused by T. verrucosum induced rapid cell mediated immunity and contributed to rapid resolution of the lesion.

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Simultaneous Removal of NO and SO2 using Microbubble and Reducing Agent (마이크로버블과 환원제를 이용한 습식 NO 및 SO2의 동시제거)

  • Song, Dong Hun;Kang, Jo Hong;Park, Hyun Sic;Song, Hojun;Chung, Yongchul G.
    • Clean Technology
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    • v.27 no.4
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    • pp.341-349
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    • 2021
  • In combustion facilities, the nitrogen and sulfur in fossil fuels react with oxygen to generate air pollutants such as nitrogen oxides (NOX) and sulfur oxides (SOX), which are harmful to the human body and cause environmental pollution. There are regulations worldwide to reduce NOX and SOX, and various technologies are being applied to meet these regulations. There are commercialized methods to reduce NOX and SOX emissions such as selective catalytic reduction (SCR), selective non-catalytic reduction (SNCR) and wet flue gas desulfurization (WFGD), but due to the disadvantages of these methods, many studies have been conducted to simultaneously remove NOX and SOX. However, even in the NOX and SOX simultaneous removal methods, there are problems with wastewater generation due to oxidants and absorbents, costs incurred due to the use of catalysts and electrolysis to activate specific oxidants, and the harmfulness of gas oxidants themselves. Therefore, in this research, microbubbles generated in a high-pressure disperser and reducing agents were used to reduce costs and facilitate wastewater treatment in order to compensate for the shortcomings of the NOX, SOX simultaneous treatment method. It was confirmed through image processing and ESR (electron spin resonance) analysis that the disperser generates real microbubbles. NOX and SOX removal tests according to temperature were also conducted using only microbubbles. In addition, the removal efficiencies of NOX and SOX are about 75% and 99% using a reducing agent and microbubbles to reduce wastewater. When a small amount of oxidizing agent was added to this microbubble system, both NOX and SOX removal rates achieved 99% or more. Based on these findings, it is expected that this suggested method will contribute to solving the cost and environmental problems associated with the wet oxidation removal method.