• KSBB Journal
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• 제10권5호
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• pp.598-603
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• 1995

현재 국내 주정공장에서 사용되고 있는 타파오카를 액화 및 당화시켜 얻은 당화액과 당화액에서 분 리된 고형분으로 회분실험과 total cell retention c culture 실험을 하였다. 당화액에 의한 회분 실험결과 에탄올 수율은 0.48 g-ethanol/g- (reducing s sugar)으로서 약 $180g/\ell$의 환원당이 36시간만에 거의 다 소모되어 약 $84g/\ell$의 에탄올이 생성되었다. 이 때 최종 효모농도는 $8.5{\times}107$ cellsjml(약$2.30g/\ell$) 이었다. 원료 중 환원당농도가 $224g/\ell$이고 희석속도가 $0.18h^{-1}$ 인 total cell retention culture 실험에서는 효모농도가 약 $40g/\ell$, 잔류 환원당농도 가 약 $15g/\ell$로서 포도당은 거의 소모된 것으로 나 타났다. 에탄올농도는 약 $81.4g/\ell$로서 이의 부피 생산성이 약 $14.7g/\ell$-h이었다. 원료 중 환원당농도가 $205g/\ell$ 이고 희석속도가 $0.2h^{-1}$이며 bleed가 있 는 cell retention culture 실험에서는 효모의 농도가 약 $22g/\ell$까지 증가하였고 에탄올농도는 $685g/\ell$부근에서 진통하였으며 생산성은 약 $13.6g/\ell$-h이 다. 약 $12g/\ell$의 잔류 환원당 중에 약 $4.5g/\ell$의 포도당이 포함되어 있었다. 타피오카 당화액으로부터 분리된 고형분을 사용한 실험을 통하여 고형분도 기 질로서 효용가치가 어느 정도 있는 것으로 판명되었으며 당화액 발효조와 별도로 고형분 발효조의 개발도 필요한 것으로 생각되었다.

• 한국약용작물학회지
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• 제11권3호
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• pp.190-194
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• 2003

두물차의 수량은 관행시비 (4회 분시)에 비해 완효성 비료 9월, 3월 분시 2회 와 3월 1회 분시는 수량차가 없었으나 9월 1회 분시는 감수되었다. 관행시비에 비해 완효성비료 2회 분시가 총 질소, 총 아미노산은 많았고, tannin과 caffeine 함량은 적은 경향이었으며 엽록소, 비타민 C, 유리당 함량과 감칠맛 주성분인 theanine 함량은 차이가 없었다. 형상, 향, 맛 등 제다품질은 완효성 비료 2회 분시가 1회 분시나 관행 시비 (4회 분시)에 비해 우수하였다. 그러나 완효성 비료처리는 토양비료성분 중 질소, 인산, 칼리의 효율성을 향상시켜 차잎 수량 및 품질을 증가시킨다고 생각된다.

• 한국연초학회지
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• 제21권1호
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• pp.82-88
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• 1999

The present study was conducted to compare the relative efficiency of two different haploid breeding methods in tobacco varietal development. A single FI hybrid plant from cross of two flue-cured cultivars of Nicotiana tabacum L., Bright Yellow4(BY4) and NC95, was used to develop the 30 anther derived dihaploid (ADH) lines and maternally-derived dihaploid (MDH) lines utilizing Nicotiana africana, respectively. As compared with mid-parent, mean of ADH lines showed increase in number of leaves, delay in days to flower and narrow in width of leaf. However, no significant differences in the other investigated characters were recognized. MDH lines also showed narrow width of leaf, while no significant differences in the other characters were observed. The variations of the investigated characters were generally greater in ADH than MDH lines. MDH lines had higher height of plant and shorter days to flower than ADH lines, while the other characters did not show remarkable differences. The degree of heritability for each of the characters observed between ADH and MDH was almost the same. The characters showing high heritability value were height of plant, number of leaves, days to flower, and yield, while those showing relatively low value were length of leaf, width of leaf, and total alkaloid content. Predicted gains from selection for increased yield were calculated for both populations(ADH, MDH) and correlated responses associated with selection for yield were estimated. Height of plant, width of leaf, days to flower, and concentration of reduceing sugar would be expected to improve with selection for yield much faster in the MDH population than in the ADH.

• Asian-Australasian Journal of Animal Sciences
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• 제15권1호
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• pp.45-54
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• 2002

Ninety lactating goats (Bachthao, Barbary, Beetal and Jamnapary breeds) were used in an experiment to investigate the replacement value of the tree fodders Flemingia (Flemingia macrophylla) and Jackfruit (Artocarpus heterophyllus). The foliages were used to replace the concentrate in diets based on chopped whole sugar cane (Sacharatum sp.), Para grass (Brachiaria mutica) and dried cassava root (Manihot esculanta). The concentrate was replaced by foliage of Jackfruit or Flemingia at 0%, 20%, 40%, 60% and 80% based on the crude protein (CP) content in the concentrate and foliages, respectively. Average milk yield was 1,617 g/day for goats fed Jackfruit compared to 1,532 g/day for those fed Flemingia. Increasing amounts of Flemingia foliage resulted in reduced dry matter intake and decreased milk yield but milk composition (CP, casein and fat content) was similar up to 60% replacement. Flemingia showed a poor potential as a supplement for lactating goats and replacement levels should not exceed 20% of the protein in the concentrate or 7.5% of the dry matter in the diet. With respect to the combination of milk production and net return over the control a CP replacement rate of 20% was the most promising. For Jackfruit there was similar feed intake and milk yield at a replacement level of 20% of CP in the concentrate (9.2% of DM intake) compared to the control diet. Milk yield at the level of 40% replacement (15% of the DM) in the diet was slightly reduced. Up to a level of CP replacement rate of 60% (21% DM in the diet) can be suggested for on-farm testing as a higher net return over the control was obtained on station.

• 한국연초학회지
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• 제14권1호
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• pp.24-32
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• 1992

The present study was conducted to compare the relative efficiency of two different haploid breeding methods in tobacco varietal development. A single F t hybrid plant from cross of two flue-cured cultivars of Nicotiana tabacum L., Bright Yellow4(BY4) and NC 95, was used to develop the 30 dihaploid lines by anther culture(F1-ADH) and maternally-derived doubled haploid utilizing Nicotiana africana(F1-MDH), respectively. As compared with mid-parent, ADH lines showed increasing in number of leaves, delaying in days to flower and narrowing in leaf width. However, no significant differences in the other characters investigated were recognized. MDH lines also showed narrow leaf width, while no significant differences in the other characters were observed. The variations of the characters investigated were generally greater in ADH than MDH lines. MDH lines had higher plant height and shorter days to flower than ADH lines, while other characters did not show remarkable differences. The degree of heritability for each of the characters observed between ADH and MDH was almost the same. The characters showing high heritability value were plant height, leaf number, days to flower, and yield, while those showing relatively low value were leaf length, leaf width, and total alkaloid content. Predicted gains from selection for increased yield were calculated for both populations(F1-ADH, F1-MDH) and correlated responses associated with selection for yield were estimated. Plant height, leaf width, days to flower, percent reducing sugar and disease resistance would be expected to improve with selection for yield much faster in the MDH population than in the ADH.

• 한국작물학회지
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• 제60권1호
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• pp.17-22
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• 2015

A total of CNU 28 hybrids were developed at the CNU Corn Breeding Lab. were evaluated to identify new cultivars in botanical characteristics, fresh yield per 10a and taste qualities. Most of these hybrids were stable in environmental stresses such as lodging, disease and insects. Stem height ranged from 115.0 to 239.3 cm, and ear height ranged from 30.7 to 107.0 cm. The ear height to stem height ratio was showed low than 50% of standard as a stable plant type to lodging. The range of ear length was 14.2 cm to 23.0 cm. Especially, CNU 13H-73 was very longest ear as a 23 cm. The fresh yield per 10a was high in purple of CNU13H-79 hybrid than control hybrid Miheuckchal, and CNU13H-73 in white hybrid was similar Yeonnong check hybrid. The 100-kernel weights in CNU13H-3 and CNU13H-9 hybrids were higher than that of the control hybrid. CNU13H-98 among hybrids had a 100-kernel weight of 20.32 g, which was heavier than that of the control hybrid Daehackchal Gold 1. The average pericarp thickness was $41.4{\mu}m$, CNU13H-46 among hybrids had a very thin pericarp as a $35.5{\mu}m$. The mean sugar content of the used hybrids was 14.95 brix%; CNU13H-73 and CNU13H-55 had higher than Mibak2 as a control hybrid.

• 한국식품과학회지
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• 제31권2호
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• pp.391-398
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• 1999

국내산 생강의 높은 원료비를 극복하면서 품질이 우수한 중간소재성 가공제품을 개발하기 위하여 효소적 추출기법을 적용하였을 때의 수율 및 품질변화를 조사하였다. 생강을 1차 착즙하고 남은 잔사를 $\alpha$-amylase로 가수분해한 후 2차 착즙하고 다시 남은 잔사에 90% 에탄올로 $30^{\circ}C$에서 1시간 추출하여 각각 착즙액을 혼합할 경우 단순 착즙액보다 동일 $^{\circ}Brix$ 기준으로 약 2.8배 정도, 펄프보다는 건물기준으로 약 5배 이상 착즙수율을 증진시킬 수 있었다. 이와 같이 제조된 최종 추출액에는 착즙액 보다 조섬유가 약 62%, 전분이 48%정도 감소하는 결과를 나타내어 작업공정의 개선이 가능함을 알 수 있었다. 또한 최종 추출액은 착즙액에 비하여 유리아미노산이 약 40% 정도 소실한 반면 유리당은 약 270% 증가하는 결과를 보여주고 있어, 생강의 효소적 추출법은 생강 가공제품 제조시 단맛을 강화하는 효과를 제공할 수 있었다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권1호
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• pp.43-47
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• 2006

Ginseng (Panax ginseng CA. Meyer) hairy root cultures, which are established via the infection of ginseng root discs with Rhizobium rhizogenes, have been used to construct profiles of both biomass growth and nutrient consumption in flask cultures. In a 250 mL shake flask culture, the maximum biomass was observed on the 59th day of the culture period, at 216.8 g (fresh wt) per liter or 11.4 g (dry wt) per liter. The hairy roots were determined to have a growth rate of 0.355 g-DW/g cells/day during the exponential growth phase and a maximum specific growth rate on day 7. Total ginseng saponin and phenolic compound contents were noted to have increased within the latter portion of the culture period. Linear correlations between increases in biomass weight and nutrient uptake were used to imply the conductivity yield $2.60g-DW/(L{\cdot}mS)$ and carbon yield 0.45 g-DW/(g sugar) in the 250 mL flask cultures. The biomass yield when two different nitrogen sources were used (ammonia and nitrate) was shown to remain approximately constant. at $0.47g-DW/(L{\cdot}mM\;NH_4$) and $0.33g-DW/(L{\cdot}mM\;NO_3$); it remained at these levels for 16 days with the ammonia. and for 24 days with the nitrate. The biomass yield when a phosphate source was used was also shown to remain approximately constant for 9 days, at $3.17g-DW/(L{\cdot}mM\;PO_4$), with an $R^2$ of 0.99.

• 한국자원식물학회지
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• 제20권3호
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• pp.258-262
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• 2007

The study was conducted to evaluate the performance of the popular isoflavone-rich soybean 'agakong' in upland and in drained-paddy fields. Analysis revealed no significant variation in terms of plant height, number of seeds per pod, number of nodes, and 100-seed weight between the two cropping system. Number of pods was significantly higher in paddy field(234.2kg 10a$^{-1}$) compared to those harvested in the upland field, which was later manifested on the yield where paddy soybeans obtained 278.1kg 10a$^{-1}$ whereas upland only obtained 179.3kg 10a$^{-1}$. This observed difference in yield was attributed to the observed higher amount of N in the paddy soil (0.907%) as compared to the upland soil (0.458%). In terms of nutritional content, protein and phytic acid contents were the only parameters that showed significant differences while oil, sugar, reducing power and fatty acids were all comparable in paddy and filed condition. Protein content was higher in upland soil (47.4%) than that of the paddy (44.9%) soil. On the opposite, phytic acid was higher in paddy (2.90%) than in upland (1.09%). This study showed that the yield of soybean is generally a factor of soil N, drained-paddy field production of soybean is comparable to upland-filed production with the benefit of increasing phytic acid content while maintaining its nutritional value.

• 현장농수산연구지
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• 제19권1호
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• pp.61-73
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• 2017

본 실험은 토양 수분이 토양 환경을 개선하고 유기물이 높은 토양과 유기물이 낮은 토양에서의 돼지감자(Jerusalem artichoke) 관수 처리와 무처리 및 백색종과 자색종에서 모종과 구근의 정식 형태에 따른 수량차이를 확인 하였다. 1. 돼지감자의 유기물 함량이 높은 토양과 유기물 함량이 낮은 토양의 수량비교는 유기물 함량이 높은 토양에서 증가를 확인하였다. 관수처리와 무관수 처리사이의 비교수량에서 관수처리에서 증가를 확인하였다. 모종정식과 구근정식사이의 수량비교에서 모종정식 처리에서 수량증가를 확인한 유의성을 나타내었다. 백색종과 자색종의 수량비교에서 백색종에서 수량 증가를 확인하였다. 2. 유기물 함량이 높은 토양에서의 관수와 무관수 사이의 수량비교는 관수처리에서 높은 수량을 확인하였다. 모종과 구근의 비교수량은 모종정식에서 수량이 증가하였다. 백색종과 자색종 사이의 비교수량에서 백색종에서 수량이 증가하였다. 3. 유기물 함량이 낮은 토양 안에서의 관수처리와 무 처리사이의 비교수량은 관수 처리에서 수량이 증가하였다. 모종과 구근정식사이의 비교수량은 모종정식에서 수량이 증가한 유의성을 나타내었다. 백색종과 자색종 정식사이의 비교 수량은 백색종 정식에서 수량이 증가하였다.