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Changes of MCPD and Chemical Components in Soy Sauce Made of Acid-hydrolyzate of Defatted Soy protein by Fermenting the Soy Sauce koji (곡자첨가에 의한 산분해간장 발효 중 MCPD 및 주요 화학성분의 변화)

  • Yoon, Bok-Man;Park, Jae-Sean;Park, Chang-Hee;Choi, Yeong-Jin;Jun, Mun-Jin
    • Korean Journal of Food Science and Technology
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    • v.30 no.2
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    • pp.419-424
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    • 1998
  • Removal of monochloropropanediol (MCPD) and improvement of quality of the soy-sauce made from acid-hydrolyzate of defatted soy protein (SAHSP) were examined by fermenting the soy-sauce with soy-sauce koji or koji plus Pediococcus soya or/and Saccharomyces rouxii. The overall fermentation process performed in this work consisted sequentially of autodigestion of soy-sauce koji $(at\;45^{\circ}C\;for\;12\;days)$, lactic acid fermentation $(at\;30^{\circ}C\;for\;14\;days\;in\;S3\;and\;S4)$, ethanol fermentation $(at\;30^{\circ}C\;for\;14\;days\;in\;S2\;and\;S4)$,and aging $(at\;25^{\circ}C\;for\;20\;days)$. At the end of the autodigestion period, the highest MCPD removal (from the initial 38.6 ppm to 1.3 ppm) was observed in the S-2. Reducing sugar contents of the S-2 and S-4 sharply decreased from the 30th day of incubation, from the initial concentration of about 5.0% to less than 0.5% at the end of the process. Total soluble nitrogen content of all the soy-sauce products slightly increased during the overall fermentating period.The level of free glutamic acid, a major amino acid that is known to determine the taste of soy-sauce was determined to be an average value of $1270{\sim}1323\;mg/100\;mL$ of soy-sauce. The results of sensory evaluation of the fermented SAHSPs show that qualities of the S-2 and S-4 samples are nearly on the same level with that of the commercially fermented soy-sauce (p<0.05). This result suggests that the fermentation process in this work, especially the process performed with S. rouxii has a good effect for removing MCPD from SAHSP and also for improving quality of the SAHSP product.

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A Study of Synthesis and Biological Function on DL-1-Aminoethylphosphonic Acid (DL-1-Aminoethylphosphonic acid의 생물학적(生物學的) 기능연구(機能硏究))

  • Kim, Sook-He;Cho, Jung-Nam;Kim, Yong-Joon
    • Journal of Nutrition and Health
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    • v.2 no.4
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    • pp.173-181
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    • 1969
  • Since 1959 ${\beta}-aminoethylphosphonic$ acid was discovered in the living organism, the biosynthesis and biological functions of aminophosphonic acids have been extensively studied. The author designed and carried out this study for 14 weeks to find out the metabolic function of Ethylaminophosphonic acid (AEP) and it's utilization in the living body. Sixty rats, thirty males and thirty females aged $40{\pm}5$ days were divided into two parts, one for alanine supplemented as control group and the other for AEP as experimental group to compare metabolic pathway of ordinary amino acid with that of AEP. Both alamine and AEP group were divived into two subgroups according to the level of supplements, 0.1% and 0.2% of the diet. The major components of the diet in this study were composed of 20% casein, 72% Sugar, 4% fat, 4% salt Mixture, and all kind of Uitamins in adeguate amount. For comparision of biological values between experimental and control group in terms of body weight, uninary nitrogen, creatinine excretion and final orgam weight, there were no statically significant difference in these respects. This meant AEP could be utilized in the body as much as alanine could. Urinary phosphorus excretion was determined by developing the blue color to read on the Spectronic 20. Statistically insignificance in the urinary phosphorus excretion between experimental and control group was observed in spite of the supplementation of phosphorus of AEP for experimental group in the diet. The level of blood phosphorus was higher in experimental group than that in control group this result supported above result. In the analysis of fat and nitrogen contents in the liver, AEP group showed slightly higher than control in both respects. But it was noteworthy 0.2% AEP group in both sex were higher than 0.1% AEP in liver fat content. Histological examinal of internal organs liiver, lung, spleen, heart, kindey, adrenal and sex organs showed no changes in all groups included in this study. The group supplemented higher level of diet. by alanine 0.2% and AEP 0.2% stayed on less body weight gain and lower liver weight. This result could be interpreted that amino acid imbalanced condition was arose in the body.

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Preparation and Characterization of Jochung, a Grain Syrup, with Apple (사과 첨가 조청의 제조 및 특성)

  • Yang, Hye-Jin;Ryu, Gi-Hyung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.1
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    • pp.132-137
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    • 2010
  • This study was performed to investigate the effect of apple and maltitol as ingredients on the quality of Jochung, a grain syrup. Four kinds of Jochung products were prepared from steamed-rice, apple juice, heated-apple sarcocarp (at $70^{\circ}C$, 60 min), and a mixture (sarcocarp : maltitol=5:1, w/w) by saccharifying (at $55^{\circ}C$, 8 hrs) with a malt (100 g/500 g rice), mixing the ingredients (steamed-rice : ingredient=5:5, w/w), filtering, and heating the filtrate (at $95^{\circ}C$, 2 hrs): product (A) with apple juice added before saccharified, product (B) with apple juice added after saccharified, product (C) with heated-apple sarcocarp added after saccharified, and product (D) with the mixture added after saccharified. The product (D) had the lowest pH value ($4.60\pm0.01$) of any other products. The contents of reducing sugar and total phenolic compound were the highest in the product (A) among all the products, which comprised $68.10\pm6.71$% and $7.36\pm0.85$ mg/g, respectively, resulting in good quality. The solidity and the dextrose equivalence had the highest value in the product (B) and the product (C), respectively. The malic acid content ($4.10\pm0.02$%) of the product (D) was the highest of any other organic acids identified by HPLC. Hunter L, a, and b values of the product (D) were the highest compared to other products. In sensory evaluation, the product (A) had generally higher score in all sensory attributes. It was concluded from the chemical and sensory evaluation that adding the apple juice before saccharified might be an effective method for manufacturing good quality rice-Jochung.

Antimicrobial, Antioxidant, and Anticoagulation Activities of Salicornia europaea seeds (함초 씨의 항균, 항산화 및 항혈전 활성)

  • Kim, Mi-Sun;Kim, Deuk Hoi;Sohn, Ho-Yong
    • Microbiology and Biotechnology Letters
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    • v.44 no.4
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    • pp.452-460
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    • 2016
  • This study was designed to develop a functional pharma-food using Salicornia europaea (SE). Tiny seeds from the mature SE were collected, and their biological activities were evaluated. The extraction yield of the seed in hot water was found to be 29.6% and the hot water extract (HWE) contained 25.7 mg/g total polyphenol (TP) and 11.5 mg/g total flavonoid (TF), which are similar to those contained in leaf and stem of SE. Among the subsequent organic solvent fractions, the ethylacetate (EA) fraction exhibited the highest content of TP (158.3 mg/g), TF (136.2 mg/g), and total sugar (228.3 mg/g). The EA fraction exhibited broad-range antibacterial activities against gram-positive bacteria, and the butanol fraction exhibited growth inhibitory effect against only Staphylococcus epidermidis. An antioxidation activity assay of the HWE and its fractions showed the EA fraction to have the highest radical scavenging activity with $RC_{50}$ values of 57.0, 29.0, and $28.9{\mu}g/ml$ against DPPH anion, ABTS cation, and nitrite, respectively. The $RC_{50}$ values of vitamin C against DPPH anion, ABTS cation, and nitrite were 10.7, 4.0, and $18.0{\mu}g/ml$, respectively, indicating that the EA fraction of SE has potent antioxidant compounds. In an anticoagulation assay, the EA fraction exhibited a 15-fold extended thrombin time at 5 mg/ml and activated partial thromboplastin time at 7 mg/ml, which are comparable to the activities of aspirin. The HWE and its fractions had no hemolysis activities against human RBCs at up to 1 mg/ml. These results suggest that the EA fraction from SE has a great potential as a new antibacterial and anticoagulation agent.

Effect of Ganoderma lucidum(Wood, Pot cultivated & Wild) Extract on the Physiological Characteristics of Saccharomyces cerevisiae (원목(原木), 병재배(甁栽培) 및 야생(野生) 영지(靈芝)의 추출물이 Saccharomyces cerevisiae의 생리에 미치는 영향)

  • Joo, Hyun-Kyu;Ha, Seung-Soo;Kim, Seong-Jo;Lee, Joong-Keun;Kim, Hyeong-Keun
    • Applied Biological Chemistry
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    • v.30 no.1
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    • pp.31-39
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    • 1987
  • This study has been investigated the effect of Ganordema lucidum extract on Saccharomyces cerevisiae growth and physiology. Sacch. cerevisiae was inoculated in Hayduck solution medium which were added 0, 0.1, 0.5, 1.0% extracts of G. lucidum and fermented at $30^{\circ}C$ for 5 days respectively. Some results about cell number, alcohol content and carbon dioxide products during fermentation are as follows: $CO_2$ evolution of yeasts by addition of extract of G. lucidum was more increased than control after the fermentation for 120 hours. It was the most abundant by addition of 1.0% extract of pot-culture G. lucidum. The cell number of yeasts during the fermentation w as more increased than control by addition of extract of G. lucidum. It was by addition of extract of pot-culture G. lucidum that the cell number of yeasts was more increased than by each addition of extract of wood-culture G. lucidum and G. lucidum. Dry weight of yeasts was systematically increased in addition of extract of pot 0.5%>pot 1.0%>wild 1.0%>wood 1.0%=wood 0.5%>wild 0.5%>wild 0.1%>pot 0.1%>wood 0.1%>control in order. It was by addition of extract of pot-culture G. lucidum that. the dry weight of yeasts was more increased than by addition of woodculture G. lucidum and wild G. lucidum. Alcohol quantity by addition of extract of G. lucidum was increased more than 3 times after the fermentation for 72 hours compared with control but there was no any difference among them after the fermentation for 120 hours. The rate of sugar-consumption and fermentation of yeast by addition of extract of G. lucidum was highly increased during the early fermentation. As times went, there was no difference among them during the subsequent fermentation.

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Studies on the Yeasts for the Brewing of Soy sauce(Part 7) -Industrial utilization of Saccharomyces rouxii $T_9$ in the brewing of soy sauce- (간장발효에 관여하는 효모에 관한 연구 (제 7 보) -Saccharomyces rouxii $T_9$을 이 용한 간장의 발효시험-)

  • Lee, Taik-Soo;Lee, Suk-Kun;Chu, Young-Ha;Shin, Bo-Kyu
    • Applied Biological Chemistry
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    • v.14 no.2
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    • pp.121-129
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    • 1971
  • During the Brewing of Soy Sauce on an industrial scale, Saccharomyces rouxii $T_9$ was cultured and added to the soy sauce mash. The comparative experiments of soy sauce mash in non-addition and addition group of yeast were examined in this report. The yeast flora and chemical composition of Soy sauce mash through out the brewing were observed and the results obtained were as follows. (1) The number of Osmophilic yeast in one ml of soy sauce mash showed $185{\times}10^3$ 1 month after mashing and $750{\times}10^3$ 4 months after mashing in case of yeast group, while presented as $98{\times}10^3$ 1 month after mashing and $394{\times}10^3$ 4 months after mashing in case of non-yeast group. And the number of Osmphilic yeast in yeast group was twice of that in non-yeast group. (2) The number of ordinary yeast of TTC red group was shown as $2132{\times}10^3\;to\;3252 ×10^3$ 5 to 6 months after mashing in case of yeast group, while presented $752{\times}10^3\;to\;1251{\times}10^3$ in case of non-yeast group. And the yeast group was shown more than non-yeast group in ordinary, red pink and pink yeast number. (3) TTC red yeast were strongly appeared in both addition and non-addition group of yeast from 1 month after mashing to 6 months after mashing. (4) Though total nitrogen, pure extract, pH and buffer action contents of soy sauce showed similiar tendency in yeast and non-yeast group, alcohol and color density contents were highly appeared in yeast group and reducing sugar content was in non-yeast group respectively. (5) By the results of Organic function test of soy sauce mash, the difference of taste quality in yeast and non-yeast group were not evidently appeared, however, the appearance and flavour of the soy sauce were better in yeast group than in non-yeast group.

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Effect of Bacillus Strains on the Chungkook-jang Processing (1) Changes of the Components and Enzyme Activities During Chungkookjang-koji Preparation (균주(菌株)를 달리한 청국장의 제조(製造)에 관(關)한 연구(硏究) 제1보(第1報)-청국장메주 발효과정중(醱酵過程中)의 성분(成分)과 효소력(酵素力)-)

  • Lee, Hyun-Ja;Suh, Jung-Sook
    • Journal of Nutrition and Health
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    • v.14 no.2
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    • pp.97-104
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    • 1981
  • In order to study the changes of components and enzyme activities during Chungkookjang-Koji preparation, the Kojies were prepared with Bacillus Natto, Bacillus subtilis and traditional method. The temperature of Koji materials during Koji preparation was very different according to the experimental group. The content of ethyl alcohol, reducing sugar, amino nitrogen and water soluble nitrogen were changed by the Koji preparing stage and experimental group. Amylase and protease activities showed on irregular change on standing and their activities were not remarkably different among the groups and appeared weakly.

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Effect of Irrigation Period on Quality of Melon(Cucumis melo L.) (관개조건(灌漑條件)이 온실재배(溫室栽培) 메론의 수량(收量) 및 품질(品質)에 미치는 영향(影響))

  • Lee, Keyong-Bo;Kim, Sun-Kwan;Yang, Chang-Hyu;Yoo, Chul-Hyun;Chon, Jang-Hyeob;Lee, Du-Ku;So, Jae-Don
    • Korean Journal of Soil Science and Fertilizer
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    • v.27 no.4
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    • pp.269-274
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    • 1994
  • An experiment was carried out to investigate the effects of irrigation period on the yield and quality of melon (Cucumis melo L.) cultivated in green house 1992~1993. Four different irrigation period was applied ; from transplanting to harvest(Irrigation I), from initial flowering to 35 days after flowering(Irrigation II), from initial flowering to 20 days after flowering(Irrigation III), from initial flowering to 5 days after flowering(Irrigation IV). The water was applied by drip irrigation when the soil water potential of 15cm depth reach at -0.5bar. The results obtained were as follows ; 1. The amount of water applied during the periods were 170.5mm, 145.0mm,126.9mm and 78.8mm for irrigation period I, II, III and IV, respectively. 2. Average evapotranspiration during the cultivation of melon was 3.31mm/day. Evapotranspiration was the highest at the period from 5days after flowering to 15days after flowering. 3. The total yield was increased with the higer amount of water applied, and the yield was in the order of Irrigation I, II, III and IV. However, the yield with economically high quality was the highest in Irrigation III. 4. The sugar content and quality of Net form were decreased with higher irrigation amount.

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Impacts of Oyster Shell and Peat Treatments on Soil Properties in Continuous Watermelon Cropping Greenhouse Plots (패화석 및 이탄 처리가 수박 연작지 토양의 특성에 미치는 영향)

  • Ahn, Byung-Koo;Lee, Jin-Ho;Lee, Young-Han
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.4
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    • pp.438-445
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    • 2010
  • Soils in continuous monoculture cropping system generally have a number of physical and chemical problems. Thus, we studied to investigate selected soil properties in continuous watermelon cropping plots with applications of different soil management practices: 1) conventional farming practice (CFP), 2) chemical fertilizer management practice (FMP), the FMP with different amounts, 0.5, 1.0, and 1.5 t $ha^{-1}$, of oyster shell meal application (FMP-OS 0.5, 1.0, and 1.5 t $ha^{-1}$), and 3) the FMP with different amounts, 2.0, 3.0, and 4.0 t $ha^{-1}$, of peat application (FMP-PT 2.0, 3.0, and 4.0 t $ha^{-1}$) and also to evaluate watermelon quality. Soil pH slightly increased only in the FMP-OS 1.5 t $ha^{-1}$ plot, while it was not changed or decreased a little in other plots. The contents of soil organic matter (SOM) expectedly increased in the FMP-PT plots, whereas it markedly decreased in the FMP-OS plots. The concentrations of exchangeable cations, $Ca^{2+}$, $Mg^{2+}$, and $K^+$, in soils were mostly dropped down in most of the FMP and FMP-PT plots. Otherwise, the exchangeable $Ca^{2+}$ concentration increased a bit in the FMP-OS plots. Also, the concentrations of water-soluble anions, $NO_3^-$, $Cl^-$, ${SO_4}^{2-}$, and ${PO_4}^{3-}$, in soils mostly declined in all the plots applied with the different management practices during the study years. Due to the cation and anion decreases, the electrical conductivity (EC) values in the soils were greatly reduced in the plots. Thus, the soil management practices applied, especially oyster shell meal and peat treatments, might be useful to control soil conditions. However, watermelon quality, such as sugar content and fruit weight, would not be associated with the soil management practices applied.

Quality Characteristics of Chun Ma (Gastrodiae rhizoma) Beverage Prepared Using Concentrated Extracts (천마추출액 농축 비율을 달리하여 제조한 천마음료의 품질특성)

  • Lee, Su-Won;Moon, Hye-Kyung;Moon, Jae-Nam;Yoon, Won-Jung;Kim, Gwi-Young
    • Food Science and Preservation
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    • v.17 no.1
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    • pp.58-65
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    • 2010
  • We investigated the effect of Chun ma (Gastrodiae rhizoma) concentrated extract on the quality of mixed beverages. Chun ma beverages prepared using different concentrated extracts were divided into four groups: GCE 5 ($5^{\circ}$ Brix concentrated extracts) GCE 10 ($10^{\circ}$ Brix concentrated extracts) GCE 15 ($15^{\circ}$ Brix concentrated extracts) and GCE 20 ($20^{\circ}$ Brix concentrated extracts). The pH values ranged from a low of 4.37 in GCE 5 to a high of 4.68 in GCE 20. Soluble solid levels in GCE 20 ($19.6^{\circ}$ Brix) were higher than in the other samples. The b (yellowness) scores and the total phenolic contents of all samples increased with increasing extract concentration. The highest total phenolic contents were seen in GCE 20 samples at 232.23 mg%. Samples did not differ markedly in antiradical activity (75.07-76.00% DPPH inhibition). Free sugar levels in GCE 20 samples and organic acid concentrations of GCE 15 samples were higher than those of other preparations. Free amino acid and mineral contents of all samples increased with increasing extract concentration. The levels of free amino acids were in the order Glu > Gly > Ser > Arg > Hylys, and the Glu content was 249.15 ug/100 g for GCE-20 samples and 61 ug/100 g in GCE-5 products. The mineral contents of all samples were in the order K > Na > Mg > Ca. Higher scores for color, flavor, and overall acceptability were found in GCE 5 products compared with other extracts. These results indicate that Chun ma beverage can be prepared in various ways, as commercially desired, with reference to the above characteristics of Chun ma materials.