• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제34권1호
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• pp.11-18
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• 2008

Human bone marrow mesenchymal stem cells are thought to be multipotent cells, which are present in adult marrow, that can replicate as undifferentiated cells and that have the potential to differentiate to lineages of mesenchymal tissues, including bone, cartilage, fat, tenden, muscle, and marrow stroma. Cells that have the characteristics of human mesenchymal stem cells could be isolated from marrow aspirates of human and animals. This study was designed to identify and characterize genes specifically expressed by osteogenic supplements -treated cells by suppression subtractive hybridization(SSH) method. The results were as follows: 1. 2 genes were upregulated genes in osteogenic diffeentiation of hMSCs, which is further proved by Northern blot analysis. 2. IGFBP-2 has been identified playing an important role in bone formation. 3. HF1 was also upregulated during osteogenic differentiation, but its role in bone formation is not clear yet.

• 벤처혁신연구
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• 제2권1호
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• pp.1-11
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• 2019

다양한 연구기관에서 4차 산업혁명을 이끌 핵심기술로 AI, IoT, Blockchain 등의 기술과 더불어 3D 프린팅 기술을 들고 있다. 3D 프린팅은 전통적인 생산방식인 subtractive manufacturing(SM)과는 대조적으로 additive manufacturing(AM)으로 그 영향력이 매우 커 혁명이라는 용어를 사용하기도 한다. 이에 본 연구에서는 3D 프린팅 산업의 특성을 경제 관점, 생산 관점, 마케팅 관점에서 전통적인 방식과 비교하여 분석하였다. 이후 비즈니스 생태계 구축 측면에서 쟁점을 분석하였다. 끝으로 향후 연구를 위한 아젠더를 제시하였다. 본 연구는 실무적으로는 3D 프린팅을 둘러싼 핵심적인 이슈 파악 및 향후 적용을 위한 단초를 제공하였다. 학문적으로는 기존의 연구 이슈에 대해 쟁점을 정리함으로써 향후 연구를 위한 틀을 제시하였다. 향후 연구에서는 이러한 논의를 토대로 실제 사례를 중심으로 실증적인 분석이 뒷받침될 필요가 있다.

• 한국전기전자재료학회논문지
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• 제26권1호
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• pp.68-72
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• 2013

The series connection-type superconducting fault current limiter (SFCL) with two magnetically coupled circuits was suggested and its effectiveness through the analysis on the current limiting and recovery characteristics was described. The fault current limiting characteristics of the proposed SFCL as well as the load voltage sag compensating characteristics according to the winding direction were investigated. To confirm the fault current limiting and the voltage sag suppressing characteristics of the this SFCL, the short-circuit tests for the simulated power system with the series connection-type SFCL were carried out. The series connection-type SFCL designed with the additive polarity winding was shown to perform more effective fault current limiting and load voltage sag compensating operations through the fast quench occurrence right after the fault appears and the fast recovery operation after the fault removes than that with the subtractive polarity winding.

• BMB Reports
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• 제41권11호
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• pp.796-802
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• 2008

Suppression subtractive hybridization (SSH) cDNA libraries of the giant tiger shrimp, Penaeus monodon, were constructed. In total, 178 and 187 clones from the forward and reverse SSH libraries, respectively, of P. monodon were unidirectionally sequenced. From these, 37.1% and 53.5% Expressed Sequence Tags (ESTs) significantly matched known genes (E-value < 1e-04). Three isoforms of P. monodon progestin membrane receptor component 1: PM-PGMRC1-s (1980 bp), PM-PGMRC1- m (2848 bp), and PM-PGMRC1-l (2971 bp), with an identical ORF of 573 bp corresponding to a deduced polypeptide of 190 amino acids, were successfully identified by RACE-PCR. Interestingly, PMPGMRC1 showed a greater expression level in testes of juvenile than broodstock P. monodon (P < 0.05). Dopamine administration ($10^{-6}$ mol/shrimp) resulted in up-regulation of PM-PGMRC1 in testes of juveniles at 3 hrs post treatment (P < 0.05), but had no effect on PM-Dmc1 (P > 0.05).

• 약학회지
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• 제25권1호
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• pp.1-7
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• 1981

Glycyrrhizin (GA) content in licorice was determined by a couple of methods using HPLC, respectively. In Method(I), GA content itself was determined from the licorice aqueous extract, while in Method (II) glycyrrhetinic acid (GHeA ; the aglicone of GA) content corresponding to the quantity of GA was measured from the chloroform extract of the hydrolyzed product of licorice aqueous extract. A reverse phase column Hibar Lichrosorb RP-18 (E. Merck) was used as the stationary phase. As the mobile phase MeOH: $H_{2}O$(0.05M-$NaH_{2}PO_{4}$)=58 : 42 solution in Method (I), and MeOH: $H_{2}O $: AcOH=78; 19: 3 solution in Method (II) were suitable, respectively. The value obtained by Method (II) appeared slightly higher than that by Method (I). The effect of some other herbal drugs on the assay of GA quantity in mixed sample was also observed in both above two methods. By Method (I) Cassiae Cortex, Rehmaniae Rhizoma, Paeoniae Radix, and Angelicae Radix gave the subtractive effect on the amount of GA compared with the value from licorice alone. In the case of Method (II) Cassiae Cortex and Rehmaniae Rhizoma appeared to have subtractive effect but Paeoniae Radix and Angelicae Radix scarcely showed any influence. Pachymae Fungus did not affect the GA content at all. It seems that glycyrrhizin in licorice interacts with certain components of other herbal drugs.

• Nuclear Engineering and Technology
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• 제49권4호
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• pp.817-824
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• 2017

Residual stress is a critical element in determining the integrity of parts and the lifetime of welded structures. It is necessary to estimate the residual stress of a welding zone because residual stress is a major reason for the generation of primary water stress corrosion cracking in nuclear power plants. That is, it is necessary to estimate the distribution of the residual stress in welding of dissimilar metals under manifold welding conditions. In this study, a cascaded support vector regression (CSVR) model was presented to estimate the residual stress of a welding zone. The CSVR model was serially and consecutively structured in terms of SVR modules. Using numerical data obtained from finite element analysis by a subtractive clustering method, learning data that explained the characteristic behavior of the residual stress of a welding zone were selected to optimize the proposed model. The results suggest that the CSVR model yielded a better estimation performance when compared with a classic SVR model.

• Nuclear Engineering and Technology
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• 제40권1호
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• pp.69-76
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• 2008

Most pressurized water reactors use Venturi flow meters to measure the feedwater flow rate. However, fouling phenomena, which allow corrosion products to accumulate and increase the differential pressure across the Venturi flow meter, can result in an overestimation of the flow rate. In this study, a soft-sensing model based on fuzzy support vector regression was developed to enable accurate on-line prediction of the feedwater flow rate. The available data was divided into two groups by fuzzy c means clustering in order to reduce the training time. The data for training the soft-sensing model was selected from each data group with the aid of a subtractive clustering scheme because informative data increases the learning effect. The proposed soft-sensing model was confirmed with the real plant data of Yonggwang Nuclear Power Plant Unit 3. The root mean square error and relative maximum error of the model were quite small. Hence, this model can be used to validate and monitor existing hardware feedwater flow meters.

• Computers and Concrete
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• 제21권6호
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• pp.697-703
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• 2018

Compressive strength is one of the most important engineering properties of concrete, and testing of the compressive strength of concrete specimens is often costly and time consuming. In order to provide the time for concrete form removal, re-shoring to slab, project scheduling and quality control, it is necessary to predict the concrete strength based upon the early strength data. However, concrete compressive strength is affected by many factors, such as quality of raw materials, water cement ratio, ratio of fine aggregate to coarse aggregate, age of concrete, compaction of concrete, temperature, relative humidity and curing of concrete. The concrete compressive strength is a quite nonlinear function that changes depend on the materials used in the concrete and the time. This paper presents an adaptive neuro-fuzzy inference system (ANFIS) for the prediction of concrete compressive strength. The training of fuzzy system was performed by a hybrid method of gradient descent method and least squares algorithm, and the subtractive clustering algorithm (SCA) was utilized for optimizing the number of fuzzy rules. Experimental data on concrete compressive strength in the literature were used to validate and evaluate the performance of the proposed ANFIS model. Further, predictions from three models (the back propagation neural network model, the statistics model, and the ANFIS model) were compared with the experimental data. The results show that the proposed ANFIS model is a feasible, efficient, and accurate tool for predicting the concrete compressive strength.

• 한국해양바이오학회지
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• 제2권3호
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• pp.192-197
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• 2007

넙치의 생체방어 및 면역관련 유전자의 대량확보를 위하여, 지질다당질로 자극한 넙치의 말초백혈구 cDNA library를 suppression subtractive hybridization 방법으로 제작하여 발현유전자의 발현 양상 및 특성을 분석하였다. 총 470개의 SSH 클론 중 기존에 밝혀진 유전자와 상동성을 보인 218개의 클론을 분석한 결과, 대표적인 전염증사이토카인 IL-1의 유인수용체인 IL-1RII가 34번의 가장 높은 빈도로 발현되었으며, IL-$1{\beta}$가 14번의 높은 발현 빈도를 보였다. 그 중 펩티도글리칸 인식 단백질및 혈관활성 장 펩타이드 유전자는 어류에서는 처음으로 보고되는 것으로, 지질다당질로 자극한 넙치의 말초백혈구 SSH cDNA library는 다양한 생체방어 및 면역관련 유전자를 포함하고 있었다.

• 한국발생생물학회지:발생과생식
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• 제6권2호
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• pp.89-96
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• 2002

성장을 멈추고 있는 원시난포(primordial follicle)에서 난포발달이 개시되어 1차난포(primary follicle)로 발달하는 조절기전은 잘 알려져 있지 않다. 이 초기 난포발달 과정에 관여하는 유전자를 알아내기 위해 suppression subtractive hybridization(SSH)을 사용하였다. 생후 1일과 5일째의 생쥐 난소로부터 얻은 cDNA로 forward와 reverse subtraction을 수행하여 각각 day1과 day5-subtracted cDNA library를 얻었다. 이를 cloning한 결과, 357개 clone의 염기 서열을 BLAST와 RIKEN을 이용해 분석하여 27개의 clone은 novel gene으로 330개의 clone은 데이터 베이스와 일치함을 알았다. 이 중에 기능이 알려진 유전자는 day1에서는 42종, day5에서는 47종이 각각 차이 나게 발현하고 있는 것으로 나타났다. Day1-subtracted cDNA library에서는 GDF8, lats2, septin2, wee1등 4개 유전자를, day5-subtracted cDNA library에서는 HSP84, laminin2, MATER, MTi7, PTP 및 wrn등 6개 유전자를 선택하여 LCM-RT-PCR방법으로 실제로 원시난포와 1차난포에서 차이 나게 발현되고 있는 것을 확인하였다. 본 연구에서 얻은 유전자 발현 양상의 결과는 앞으로 생쥐뿐만 아니라 사람 난소에서 primordial-primary follicle transition에 관여하는 기전을 연구하는데 중요한 정보를 제공할 수 있을 것으로 사료된다.