• Title/Summary/Keyword: subtractive

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Printed polymer and a-Si TFT backplanes for flexible displays

  • Street, R.A.;Wong, W.S.;Ready, S.E.;Chabinyc, M.L.;Arias, A.C.;Daniel, J.H.;Apte, R.B.;Salleo, A.;Lujan, R.;Ong, Beng;Wu, Yiliang
    • 한국정보디스플레이학회:학술대회논문집
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    • 2005.07a
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    • pp.697-699
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    • 2005
  • The need for low cost flexible TFT display backplanes has focused attention on new processing techniques and materials. We have developed backplane technology based entirely on jet-printing, using a combination of additive and subtractive processing, and have applied this technique to both amorphous silicon and polymer TFT arrays.

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Identification of differentially expressed Genes by methyl mercury in neuroblastoma cell line using SSH

  • Kim, Youn-Jung;Chang, Suk-Tai;Yun, Hye-Jung;Ryu, Jae-Chun
    • Proceedings of the Korean Society of Toxicology Conference
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    • 2002.11b
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    • pp.153-153
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    • 2002
  • Methylmercury (MeHg), one of the heavy metal compound, can cause severe damage to the central nervous system in humans. Many reports have contributed MeHg poisoning to contaminated foods and release into the environment. Despite many studies on the pathogenesis of MeHg-induced central neuropathy, no useful mechanism of toxicity has been established.(omitted)

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Assessment of Internal Fitness on Resin Crown Fabricated by Digital Light Processing 3D Printer

  • Kang, Wol;Kim, Min-Su;Kim, Won-Gi
    • Journal of dental hygiene science
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    • v.19 no.4
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    • pp.238-244
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    • 2019
  • Background: Recently, three-dimensional (3D) printing has been hailed as a disruptive technology in dentistry. Among 3D printers, a digital light processing (DLP) 3D printer has certain advantages, such as high precision and relatively low cost. Therefore, the latest trend in resin crown manufacturing is the use of DLP 3D printers. However, studies on the internal fitness of such resin crowns are insufficient. The recently introduced 3D evaluation method makes it possible to visually evaluate the error of the desired area. The purpose of this study is to evaluate the internal fitness of resin crowns fabricated a by DLP 3D printer using the 3D evaluation method. Methods: The working model was chosen as the maxillary molar implant model. A total of 20 resin crowns were manufactured by dividing these into two groups. One group was manufactured by subtractive manufacturing system (PMMA), while the other group was manufactured by additive manufacturing system, which uses a DLP 3D printer. Resin crowns data were measured using a 3D evaluation program. Internal fitness was calculated by root mean square (RMS). The RMS was calculated using the Geomagic Verify software, and the mean and standard deviation (SD) were measured. For statistical analysis, IBM SPSS Statistics for Windows ver. 22.0 (IBM Corp., USA) was used. Then, independent t-test was performed between the two groups. Results: The mean±SD of the RMS were 41.51±1.51 and 43.09±2.32 for PMMA and DLP, respectively. There was no statistically significant difference between PMMA and DLP. Conclusion: Evaluation of internal fitness of the resin crown made using a DLP 3D printer and subtractive manufacturing system showed no statistically significant differences, and clinically acceptable results were obtained.

Gene Expression Profiling in the Pituitary Gland of Laying Period and Ceased Period Huoyan Geese

  • Luan, Xinhong;Cao, Zhongzan;Xu, Wen;Gao, Ming;Wang, Laiyou;Zhang, Shuwei
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.7
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    • pp.921-929
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    • 2013
  • Huoyan goose is a Chinese local breed famous for its higher laying performance, but the problems of variety degeneration have emerged recently, especially a decrease in the number of eggs laid. In order to better understand the molecular mechanism that underlies egg laying in Huoyan geese, gene profiles in the pituitary gland of Huoyan geese taken during the laying period and ceased period were investigated using the suppression subtractive hybridization (SSH) method. Total RNA was extracted from pituitary glands of ceased period and laying period geese. The cDNA in the pituitary glands of ceased geese was subtracted from the cDNA in the pituitary glands of laying geese (forward subtraction); the reverse subtraction was also performed. After sequencing and annotation, a total of 30 and 24 up and down-regulated genes were obtained from the forward and reverse SSH libraries, respectively. These genes mostly related to biosynthetic process, cellular nitrogen compound metabolic process, transport, cell differentiation, cellular protein modification process, signal transduction, small molecule metabolic process. Furthermore, eleven genes were selected for further analyses by quantitative real-time PCR (qRT-PCR). The qRT-PCR results for the most part were consistent with the SSH results. Among these genes, Synaptotagmin-1 (SYT1) and Stathmin-2 (STMN2) were substantially over-expressed in laying period compared to ceased period. These results could serve as an important reference for elucidating the molecular mechanism of higher laying performance in Huoyan geese.

Identification of Genes that are Induced after Cadmium Exposure by Suppression Subtractive Hybridization

  • 이미옥
    • Proceedings of the Korea Society of Environmental Toocicology Conference
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    • 2003.05a
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    • pp.107-107
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    • 2003
  • The heavy metal cadmium is a xenobiotic toxicant of environmental and occupational concern and it has been classified as a human carcinogen. Inhalation of cadmium has been implicated in the development of emphysema and pulmonary fibrosis, but, the detailed mechanism by which cadmium induces adverse biological effects is not yet known. Therefore, we undertook the investigation of genes that are induced after cadmium exposure to illustrate the mechanism of cadmium toxicity For this purpose, we employed the polymerase chain reaction-based suppression subtractive hybridization technique. We identified 29 different cadmium-inducible genes in human peripheral mononuclear cells, such as macrophage migration inhibitory factor, lysophosphatidic acid acyltransferase-${\alpha}$, enolase-1${\alpha}$, VEGF, Bax, neuron-derived orphan receptor-1, and Nur77, which are known to be associated with inflammation, cell survival, and apoptosis. Induction of these genes by cadmium treatment was further confirmed by semi-quantitative reverse-transcription polymerase chain reaction. Further, we found that these genes were also induced after cadmium exposure in normal human lung fibroblast cell line, WI-38, suggesting potential use of this induction profile to monitor cadmium toxicity in the lung. Next, Nur77, one of cadmium-inducible genes, was further studied since the products of Nur77 are known to be involved in the apoptotic process of lung cells. Following cadmium treatment, Nur77 gene expression was increased at protein-level in A549 cells. Consistently, the reporter containing Nur77 binding sequence was activated by 2.5-fold after exposure to cadmium in reporter gene analysis by transient transfection experiments. When the plasmid encoding dominant negative Nur77 that represses the transcriptional function of wild-type Nur77 was transfected into A549 cells, the expression of Bax was significantly reduced, suggesting that induction of Nur77 was an important process in cadmium-induced apoptosis in the cells. Cadmium induced the expression of Nur77 in vivo, confirming the relevance of the data obtained in viro. Together our results suggest that Nur77 gene expression in exposure to cadmium leads apoptosis of lung cells which may cause pathological changes in lung.

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Analysis on Fault Current limiting and Recovery Characteristics of Flux-Lock Type Superconducting Fault Current Limiter According to Increase of Applied Voltage (전압증가에 따른 자속구속형 초전도 한류기의 전류제한 및 회복특성 분석)

  • Oh, Kum-Gon;Han, Tae-Hee;Cho, Yong-Sun;Cho, Hyo-Sang;Choi, Myoung-Ho;Han, Young-Hee;Sung, Tae-Hyun
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.21 no.8
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    • pp.107-112
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    • 2007
  • The flux-lock type SFCL consists of transformer with primary and secondary windings connected to a superconducting element in serial. It can be divided into the subtractive and the additive polarity windings according to the winding direction. It could change the fault current limiting characteristics according to the inductance ratio between the coil 1 and coil 2. We investigated the voltage-current characteristics of the flux-lock type SFCL according to the increment of applied voltage. When the applied voltage of the SFCL with the subtractive and the additive polarity windings was increased a initial limiting current ($I_{ini}$) and the quench time of the superconducting element were increased. The recovery time of the superconducting element was increased by increment of applied voltage. Therefore, it was confirmed that recovery characteristics in the flux-lock type SFCL were largely dependent on the consumed energy of a superconducting element because of increment of the consumption power into the superconducting element.