• Korean Journal of Veterinary Service
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• v.28 no.1
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• pp.1-21
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• 2005

Pullorum disease and Fowl typhoid are kind of poultry specific disease for poultry. The peculiar character of these poultry specific diseases is that it can be infected by transmitting vertically and horizontally, also it is hard to be discovered by clinical sign, and pathology or immunology. So, to develop the PCR method which distinguishes these two genetically similar diseases of separated the specific DNA fragment from each strain and use it for differential diagnosis by subtraction PCR method. Standard strain of S gallinarum and S pullorum, and field isolation strain were verified by biochemistry, It confirmed existence of plasmid by using the PFGE. Then, Isolated DNA from it and used it as materials for the experiment. After cutting genomic DNA of two strains by using Sau 3Al, It ligated primer to tester DNA for PCR amplification and separated specific DNA fragment bacteria with method of subtraction PCR. And, It confirmed that it is a piece of unique DNA in every bacteria using base sequence of separated DNA fragment. 1. The six specific DNA fragment were separated from the DNA of S gallinarum and S pullorum by the subtraction PCR method. 2. In the result of comparison after setting base sequence of each fragment, each separated base sequence of DNA fragment they did not correspond to each other 3. As the result of each DNA fragment is derived from the each strain of DNA, and there was no homology of genomic DNA level in mutual. 4. The fragment originated in plasmid and includes S pullorum did not separate. 5. In the result of searching base sequence in Genebank, it partially shows homology in Salmonella enterica, S typhimurium, S dublin, Escherichia coli, Shigella flexneri, Yersinia pestis, Klebsiella pneumoniae. 6. Primer design by S gallinarum DNA 2, 3 fragment used PCR, They are positive reaction in only S gallinarum at 276, 367 bp position.

• KIISE Transactions on Computing Practices
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• v.22 no.3
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• pp.133-138
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• 2016

Motion detection algorithms based on difference image are classified into background subtraction and previous frame subtraction. 1) Background subtraction is a convenient and effective method for detecting foreground objects in a stationary background. However in real world scenarios, especially outdoors, this restriction, (i.e., stationary background) often turns out to be impractical since the background may not be stable. 2) Previous frame subtraction is a simple technique for detecting motion in an image. The difference between two frames depends upon the amount of motion that occurs from one frame to the next. Both these straightforward methods fail when the object moves very "slightly and slowly". In order to efficiently deal with the problem, in this paper we present an algorithm for motion detection that incorporates "reflected light area" and "difference image". This reflected light area is generated during the frame production process. It processes multiplex difference image and AND-arithmetic of bitwise. This process incorporates the accuracy of background subtraction and environmental adaptability of previous frame subtraction and reduces noise generation. Also, the performance of the proposed method is demonstrated by the performance assessment of each method using Gait database sample of CASIA.

• Imaging Science in Dentistry
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• v.39 no.1
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• pp.35-39
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• 2009

Purpose: The purpose of the experiment was to evaluating the diagnostic ability of dental caries detection using digital subtraction in the artificial caries activity model. Materials and Methods: Digital radiographies of five teeth with 8 proximal surfaces were obtained by CCD sensor (Kodak RVG 6100 using a size #2). The digital radiographic images and subtraction images from artificial proximal caries were examined and interpreted. In this study, we proposed novel caries detection method which could diagnose the dental proximal caries from single digital radiographic image. Results: In artificial caries activity model, the range of lesional depth was $572-1,374{\mu}m$ and the range of lesional area was $36.95-138.52mm^2$. The lesional depth and the area were significantly increased with demineralization time (p<0.001). Furthermore, the proximal caries detection using digital subtraction radiography showed high detection rate compared to the proximal caries examination using simple digital radiograph. Conclusion: The results demonstrated that the digital subtraction radiography from single radiographic image of artificial caries was highly efficient in the detection of dental caries compared to the data from simple digital radiograph.

• School Mathematics
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• v.11 no.3
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• pp.479-496
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• 2009

Some children can construct a basic concept of addition and subtraction during the preschool years. Children start to experience mathematics via numbers and their of operations and contact with various contexts of addition and subtraction. In special, word problems reflect mathematics which is appliable to real life. In this paper, I analyse the types of word problems in text book and its students' responses. First, I analyse the types of addition word problems which consist of change add-into situations and part-part-whole situations. Second, I analyse the types of subtraction word problems which consist of change take-away situations, compare situations and equalize situations. Third, I analyse the students' responses by the types of word problems in addition and subtraction. And 115 2nd grade elementary school students participated in this survey. The following results have been drawn from this study. First, the proposition of word problems of part-part-whole situations is higher than that of change add-into situations and the proposition of word problems of take-away situations is higher than that of compare situations and equalize situations. According to the analysis about students' responses, It is no difference between change add-into situations and part-part-whole situations. But the proposition of word problems of take-away situations is higher than that of compare situations and equalize situations. This results from word problems which contain unnecessary information in problem. So, we have to present the various word problems to students.

• The Korean Journal of Nuclear Medicine
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• v.29 no.4
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• pp.465-472
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• 1995

An accurate preoperative localization in patients with primary hyperparathyroidism is important for successful surgical intervention. There are many methods for the localization of the primary hyperparathyroidism such as ultrasonography, computerized tomography(CT), magnetic resonance imaging(MRI), angiography and $^{201}T1/^{99m}Tc$ subtraction scan. Among them $^{201}T1/^{99m}Tc$ subtraction scan is known as the most accurate tool for preoperative localization. Recently $^{99m}Tc$-Sestamibi has been used for parathyroid gland imaging. We experienced 19 primary hyperparathyroidisms accurately localized with $^{201}T1/^{99m}Tc$ subtraction imaging and $^{99m}Tc$-Sestamibi. The sensitivities of the $^{99m}Tc$-Sestanibi, $^{201}T1/^{99m}Tc$ subtraction imaging, CT and US were 100%(5/5), 89.5%(17/19) and 61.7(12/19) respectively. With the combination of $^{99m}Tc$-Sestamibi and $^{201}T1/^{99m}Tc$ subtraction imaging we could localize with 100% accuracy. Although the case number is small, this study suggests that the $^{99m}Tc$-Sestamibi parathyroid scan is very useful and easy to use for preoperative localization in primary hyperparathyroidism.

• Fire Science and Engineering
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• v.32 no.5
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• pp.95-104
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• 2018

The 119 emergency medical services(EMS) personnel studied in this research are constantly exposed to traumatic events, which can lead to a variety of psychosocial problems and poor quality of life. In this study, we examined the effects of the Mind Subtraction Meditation Program on post-traumatic growth and resilience of 119 EMS personnel. In this study, we measured the conditions of 26 EMS personnel of A firefighting headquarters based on post-traumatic growth and resilience before and after implementing the short-term intensive for 2 nights and 3 days in $201^*$. The results showed that there was a significant increase in post-traumatic growth from $2.85({\pm}.67)$ to $3.60({\pm}.72)$ and resilience from $2.45({\pm}.39)$ to $2.83({\pm}.48)$ of the subjects between before and after the Mind Subtraction Meditation. In conclusion, the Mind Subtraction Meditation Program was effective in improving the posttraumatic growth and resilience of 119 EMS personnel. Therefore, Mind Subtraction Meditation Program could be proposed as a mental health promotion program for EMS personnel.

• Fire Science and Engineering
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• v.33 no.5
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• pp.118-129
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• 2019

Firefighters are high-risk group for posttraumatic stress disorder because they are repeatedly exposed to traumatic events. In this study, we examined the effects of a short-term, intensive, Mind subtraction meditation-based healing program on the ruminations and posttraumatic stress disorder symptoms of firefighters. In 2019, we used a survey questionnaire to assess the intrusive ruminations of 80 firefighters at a firefighting headquarters before and after implementing the Mind subtraction meditation-based healing program and personal journals over a period of two nights and three days. The results showed that there was a significant decrease in posttraumatic stress disorder symptoms, from 32.40 (± 14.67) to 30.31 (± 13.76) among the male subjects, and a significant decrease in posttraumatic stress disorder symptoms, from 32.03 (± 13.31) to 27.33 (± 10.68) and intrusive ruminations, from 5.21 (± 7.32) to 3.41 (± 6.30), among the female subjects. In conclusion, the Mind subtraction meditation-based healing program was effective in reducing the posttraumatic stress disorder symptoms and intrusive ruminations of firefighters. Therefore, the Mind subtraction meditation-based healing program could be proposed as a mental health promotion program for firefighters.

• The KIPS Transactions:PartB
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• v.15B no.1
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• pp.25-36
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• 2008

In this paper, we present a robust hand recognition approach to sudden illumination changes. The proposed approach constructs a background model with respect to hue and hue gradient in HSI color space and extracts a foreground hand region from an input image using the background subtraction method. Eighteen features are defined for a hand pose and multi-class SVM(Support Vector Machine) approach is applied to learn and classify hand poses based on eighteen features. The proposed approach robustly extracts the contour of a hand with variations in illumination by applying the hue gradient into the background subtraction. A hand pose is defined by two Eigen values which are normalized by the size of OBB(Object-Oriented Bounding Box), and sixteen feature values which represent the number of hand contour points included in each subrange of OBB. We compared the RGB-based background subtraction, hue-based background subtraction and the proposed approach with sudden illumination changes and proved the robustness of the proposed approach. In the experiment, we built a hand pose training model from 2,700 sample hand images of six subjects which represent nine numerical numbers from one to nine. Our implementation result shows 92.6% of successful recognition rate for 1,620 hand images with various lighting condition using the training model.

• Journal of the Chungcheong Mathematical Society
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• v.26 no.2
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• pp.297-308
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• 2013

We define one-sided quadrangular fuzzy sets, a left quadrangular fuzzy set and a right quadrangular fuzzy set. And then we generalize the results of addition, subtraction, multiplication, and division based on the Zadeh's extension principle for two one-sided quadrangular fuzzy sets. In addtion, we find the condition that the result of addition or subtraction for two one-sided quadrangular fuzzy sets becomes a triangular fuzzy number.

• Journal of the Chungcheong Mathematical Society
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• v.27 no.2
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• pp.277-286
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• 2014

We define the pentagonal fuzzy sets and generalize the results of addition, subtraction, multiplication, and division based on the Zadeh's extension principle for two pentagonal fuzzy sets. In addtion, we find the condition that the result of addition or subtraction for two pentagonal fuzzy sets becomes a triangular fuzzy number and give some example.