• Title/Summary/Keyword: subspecies

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Identification and Biochemical Characterization of a New Xylan-degrading Streptomyces atrovirens Subspecies WJ-2 Isolated from Soil of Jeju Island in Korea (제주도 토양으로부터 자일란 분해 Streptomyces atrovirens subspecies WJ-2 동정 및 효소의 생화학적 특성 규명)

  • Kim, Da Som;Bae, Chang Hwan;Yeo, Joo Hong;Chi, Won-Jae
    • Microbiology and Biotechnology Letters
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    • v.44 no.4
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    • pp.512-521
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    • 2016
  • A bacterial strain was isolated from a soil sample collected on Jeju Island, Korea. The strain, designated WJ-2, exhibited a high xylanase activity, whereas cellulase activity was not detected. The 16S rRNA gene sequence of WJ-2 was highly similar to type strains of the genus Streptomyces. A neighbor-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain WJ-2 is phylogenetically related to Streptomyces atrovirens. Furthermore, DNA-DNA hybridization analysis confirmed that strain WJ-2 is a novel subspecies of Streptomyces atrovirens. The genomic DNA G+C content was 73.98 mol% and the major fatty acid present was anteiso-C15:0 (36.19%). The growth and xylanase production of strain WJ-2 were significantly enhanced by using soytone and xylan as nitrogen and carbon sources, respectively. Crude enzyme preparations from the culture broth of strain WJ-2 exhibited maximal total xylanase activities at pH 7.0 and $55^{\circ}C$. Thin-layer chromatography analysis revealed that the crude enzyme degrades beechwood xylan to yield xylobiose and xylotriose as the principal hydrolyzed end products.

Numerical Taxonomic Study on Five Species of Genus Apodemus in East Asia (동아시아산 붉은쥐속 5종의 형태형질의 수리분류학적 연구)

  • 고흥선;천태영;김영기
    • Animal Systematics, Evolution and Diversity
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    • v.15 no.2
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    • pp.165-174
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    • 1999
  • Specimens of five species of Apodemus(A. agrarius, A. chevrieri, A. draco, A. latronum, and A. peninsulae) from east Asia (Korea and China) were used for numerical taxonomic analyses with 31 external and cranial characters. Two groups and eight subgroups within them were recognized: [I; a large-size form; (A. agrarius chejuensis), (A. chevrieri), (A. draco orestes and A. latronum), and(A. peninsulae peninsulae and A. p. preator)], [II; a small-size form; (A. agrarius coreae), (A. agrarius manchuricus, A. a. ningpoensis, and A. a. pallidior), (A. draco draco), and (A. peninsulae sowerbyi)]. Conclusions based on morphometric analyses are such as followings: 1) Morphometric characters appeared not to be useful in order to distinguish two subgenera in Apodemus (Apodemus and Alsomys), 2) further analysis is necessary to determine whether or not A. draco and A. latronum are conspecific, and 3) A. a. chejuensis is in morphometric characters the largest form among five species of Apodemus studied. Moreover, it is confirmed: 1) A. agrarius, A. chevrieri, and A. peninsulae are distinct species, 2) A. draco and A. latronum are not conspecific with A. peninsulae. 3) Three forms of A. agrarius (large-size form, medium-size form, and small-size form) can be recognized as three subspecies [(A. a. chejuensis), (A. agrarius coreae), and (A, a, ningpoensis including A. a. manchuricus and A. a. pallidior)], 4)A. draco draco and A. draco orestes are distinct subspecies, 5) A. peninsulae peninsulae (including A. p. preator) and A. p. sowerbyi are distinct subspecies.

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Phylogenetic Analysis of Native Vigna sinensis in Korea Using DNA Sequence of Internal Transcribed spacer (ITS) Region (토종 갓끈동부의 ITS1, 5.8S 및 ITS2의 염기서열을 이용한 계통 분석)

  • Seo, Pil-Soo;Lee, Sook-Young;Shin, Yong Kook
    • Journal of Life Science
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    • v.27 no.3
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    • pp.351-354
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    • 2017
  • Cowpea (Vigna unguiculata (L.) Walp.) is recognized as a potential source of protein and other nutrients. The genus Vigna includes 100 wild species of plants. Especially, Vigna unguiculata includes annual cowpeas (ssp. unguiculata) and ten wild perennial subspecies. DNA sequence of internal transcribed spacer (ITS) region was determined for Vigna sinensis, one of native plant, which was found in recent but thought to have gone extinct in Korea. The seeds of Vigna sinensis used in this study were donated from Dong-Young Jo. The DNA sequence of ITS-5.8S-ITS2 for Vigna sinensis obtained from this study was deposited as Vigna sinensis AY195581 on GenBank of NCBI (National Center for Biotechnology Information). We investigated the sequence-based phylogenetic relationships of plants related and clarified its taxonomical position. DNA similarities among subspecies including Vigna unguiculata showed the range 98 to 100% in sequence-based phylogenetic analysis using total 507 base pairs of ITS1, 5.8S and ITS2. Vigna unguiculata and subspecies were grouped independently as one cluster from other Vigna species used in the phylogenetic analysis. In this study, based on the phylogenetic analysis using the ITS1-5.8S-ITS2 sequence of Vigna sinensis, it may be concluded to be classified to one of Vigna unguiculata substrains.

Analysis of Sugars Content by Genotypes in 82 Radish (Raphanus sativus L.) (무 유전자원 82 계통의 유리당 함량 분석)

  • Seo, Mi-Suk;Chung, Joon-Hui;Park, Beom-Suk;Kim, Jung Sun
    • Korean Journal of Plant Resources
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    • v.31 no.5
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    • pp.453-465
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    • 2018
  • Radish (Raphanus sativus L.) is a species of the Brassicaceae family and an important root vegetable crop, produced worldwide. A total of 82 radish accessions with various morphological and physiological characteristics analyzed for total sugars content. These accessions includes five subspecies and classified as wild, wild-relative, traditional and improved cultivar. The four sugars, glucose, fructose, sucrose and maltose, showed various contents in 82 accessions. Total sugar content ranged from 5.64 to 46.68 mg/g and showed average 25.33 mg/g. Total sugar content was not statistically significant among the five subspecies, but individual sugar ratio varied. The wild, wild-relatives and traditional cultivars were not significantly among average total sugars content compared with improved cultivars. On the other hand, the wild and traditional cultivars were showed high ratio of individual sugars. These results could be valuable information for the development of new radish cultivars and regulation of sugars biosynthesis in radish.

Vascular Plants and Deterioration Status of Osongjae, Ogongjae, Shinyongjae and Eodujae Reservoir in Jeonju (전주 오송제, 오공제, 신용제, 어두제 소류지의 관속식물상과 훼손실태)

  • Beon, Mu-Sup;Oh, Hyun-Kyung;Kim, Jae-Byung
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.9 no.4
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    • pp.24-35
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    • 2006
  • The vascular plants of the studied area was listed 384 taxa of 90 families, 234 genera, 331 species, 1 subspecies, 45 varieties and 7 forms. The flora of each reservoir, 285 taxa of 83 families, 190 genera, 247 species, 1 subspecies, 30 varieties, 7 forms in Osongjae, 117 taxa of 39 families, 87 genera, 100 species, 17 varieties in Ogongjae, 83 taxa of 30 families, 67 genera, 73 species, 8 varieties, 2 forms in Shinyongjae and 86 taxa of 33families, 70 genera, 75 species, 1 subspecies, 10 varieties in Eodujae. Based on the list of the rare plants by the Forest Research Institute, 3 taxa were recorded in the studied areas; Magnolia kobus (Preservation priority order : No. 187), Penthorum chinense (No. 144), Prunus yedoensis (No. 110) and based on the list of Korean endemic plants, 4 taxa were recorded; Populus tomentiglandulosa, Clematis trichotoma, Prunus yedoensis, Paulownia coreana. Specific plant species by floral region were total 15 taxa; Prunus yedoensis in class V, 2 taxa (Carex idzuroei, Magnolia kobus) in class IV, 2 taxa (Monochoria korsakowi, Poncirus trifoliata ) in class III, 10 taxa (Sagittaria aginashi, Salix glandulosa, Clematis brachyura, Nymphoides peltata, etc.) in class I. The naturalized plants in this site were 13 families, 29 genera, 43 species, 1 varieties, 44 taxa and naturalization rate was 11.5% and based on the list of an ecosystem disturbance plants, 3 taxa were recorded in the studied areas : Paspalum distichum, Solanum carolinense, Ambrosia artemisiifolia var. elatior. The hydrophytes was listed 31 taxa of 15 families, 20 genera, 29 species, 2 varieties and Eleocharis mamillata var. cyclocarpa, Penthorum chinense, Soirodela polyrhiza were recorded in Osongjae, Sagittaria aginashi, Eleocharis kuroguwai, Scirpus juncoides, Scirpus triqueter, Monochoria korsakowi, Monochoria vaginalis var. plantaginea were recorded in Ogongjae. Besides, Scirpus fluviatilis, Trapa pseudoincisa were recorded in Shinyongjae and Scirpus triangulatus, Nymphoides peltata, Myriophyllum verticillatum were recorded in Eodujae. This site is located in the urban area and ecosystem-disturbing wild animal and plant has been imported here, therefore ecosystem has been disturbed more and more. The wetland here rapidly has changed into upland, so it needs conservation measures through long-term monitoring.

Systematic Studies on Korean Rodents : VIII. Analyses of Morphometric Characters, Chromosomal Karyotype, and Mitochondrial DNA Restriction Fragments in Siberian Chipmunks from Korea (Tamias sibiricus barberi Johnson and Jones), with the Comparison of Morphometric Characters of Siberian Chipmunks from Manchuria (Tamias sibiricus orientalis Bonhote) (한국산 설치류의 계통분류학적 연구: 8. 다람쥐(Tamias sibiricus barberi Johnson and Jones)의 형태적 형질, 염색체 핵형 및 미토콘드리아 DNA절단 단편의 분석과 만주다람쥐(Tamias sibiricus orientalis Bonhote)와의 형태적 형질의 비교)

  • Hung Sun Koh
    • Animal Systematics, Evolution and Diversity
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    • v.10 no.2
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    • pp.231-243
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    • 1994
  • Samples of Siberian chipmunks (Tamias sibiricus borberi ) from Korea were analyzed by multivariate, air-drying , and blot-hybridization methods in order to determine the degree of variations of morphological characters, chromosomal karyotype, and mtDNA restriction fragment patterns, respectively, Moreover, morphometric characters of samples of Siberian chipmunks from Manchuria(T.sibiricus orientalis) were also analyzed in order to clarify the taxonomic status of the subspecies barberi. Siberian chipmunks from six localities in Korea were in morphometric characters more or less similar with one another and Siberian chipmunks from Manchuria were not so distinct enough to from a subgroup. Siberian chipmunks from Four localities in Korea were identical in their karyotypes (2n=38) : samples from three localities in Korea were nore or less similar in their mtDNA fragment patterns. Siberian chipmunks from Korea appeared to be a omogeneous population and as noted by Corbet (1978) subspecies barberi is the synonym of subspecies orientalis. The scientific name of Siberian chipmunks from Korea and Manchuria is T. sibiricus orientalis, although systematic studies with the samples from North korea, Manchuria and China will be necessary for their taxonomic reconsideration.

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Morphometric Analyses of the Three Subspecies of Striped Field Mouse, Apodemus agrarius Pallas (Mammalia: Rodentia) from Far Eastern Asia: Taxonomic Status of North Korean Striped Field Mice (극동아시아에 서식하고 있는 등줄쥐 Apodemus agrarius Pallas (포유강 :설치 목) 3아종의 형태 형질의 분석: 북한산 등줄쥐의 분류학적 위치)

  • Koh, Hung-Sun;G. Csorba;M.P. Tiunov;G. Tikhonova
    • Animal Systematics, Evolution and Diversity
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    • v.14 no.4
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    • pp.327-334
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    • 1998
  • Four external and 27 cranial characters of three subspecies of striped field mice (Apodemus agrarius) from 15 regions in far eastern Asia(South Korea, North Korea, northeastern China, and far eastern Russia) were analyzed by multivariate methods in order to determine taxonomic status of striped field mice from North Korea. Three subgroups were recognized: a large-size form from two localities in South Korea(Cheju Island, Wando Island): a medium-size form from eight localities in South Korea and North Korea(Jindo Island, Kunsan, Mt. Chirisan, Cheongju, Mt. Weoleksan, Mt. taebaksan; Haeju, Sineuiju); a small-size form from five localities in North Korea, northeastern China, and far eastern Russia (Mt. Kumkangsan, Mt. Myohyangsan; Jirin, Yichun; Vladivostok). It is confirmed that the large-size form, the medium-size form, and the small-size form are subspecies chejuensis, coreae, and manchuricus, respectively, as noted by Koh et al.(1997). It is revealed that striped field mice(the medium-size form) from western region of North Korea (Haeju, Sineuiju) is classified into A. agrarius coreae, as noted by Thomas(1908), but striped field mice(the small-size form) from eastern region of North Korea(Mt. Kumkangsan, Mt. Myohyangsan) is A. agrarius manchuricus.

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The Vascular Plants of Bigeumdo and Dochodo Island (비금·도초도의 식물상)

  • Jang, Young-Jong;Jung, Su Young;Kang, Eun Su;Lee, Kang-Hyup;Lee, Jung Sim;Son, Dong Chan
    • Korean Journal of Plant Resources
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    • v.35 no.5
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    • pp.614-640
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    • 2022
  • We investigated vascular plants of Bigeumdo and Dochodo Island in Shinan-gun, Jeollanam-do, Korea. By referring to 4 field surveys between June and September in 2021, a total of 490 taxa were identified and grouped into 113 families, 332 genera, 452 species, 6 subspecies, 27 varieties, and 3 forms. Among those observed, 232 taxa comprising 84 families, 189 genera, 220 species, 4 subspecies, 8 varieties, 4 forms were observed in Bigeumdo, 428 taxa comprising 107 families, 305 genera, 397 species, 5 subspecies, 23 varieties and 3 forms were observed in Dochodo. Among them, 7 taxa were Korean endemic plants. Protected species designated by the Ministry of Environment were 2 taxa of level II. In terms of threated plants according to the IUCN Red List Categoty, there were 5 taxa, consisting of 1 endangered species (EN), 3 vulnerable species (VU), 1 Near Threatened (NT). In total, there are 36 taxa of floristic regional indicator plants, including 1 level V taxa, 5 level IV taxa, 28 level III taxa. In Korea, 3 taxa were the northern distributional limit to Bigeumdo and Dochodo. Alien plants were 58 taxa.

Flora of Oesorak in Soraksan National Park (설악산 국립공원 외설악의 관속식물상)

  • Kim, Yong-Shik;Kang, Ki-Ho;Bae, Jun-Kyu;Shin, Hyun-Tak
    • Korean Journal of Environment and Ecology
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    • v.10 no.2
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    • pp.211-239
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    • 1997
  • The flora of Oesorak in the Soraksan National Park including Chombongsan(Mountain) and Kwanmobong(Peak) were surveyed from February to August, 1996. These areas have very rich and diverse flora;620 taxa with 89 families, 321 genera, 526 species, 2 subspecies, 89 varieties and 3 forms in the Oesorak, 404 taxa with 251 genera, 350 species, 1 subspecies, 51 varieties and 2 forms in the Chombongsan(Mountain), 286 taxa with 206 genera, 233 species, 1 subspecies, 50 varieties and 2 forms in Kwanmobong(peak). The Oesorak had very distinct floristic characteristics such as the wild habitats of Asarum maculatum(Aristolochiaceae) and Ilex macropoda(Aquifoliaceae). In the phyorgeographical point of view, the six species such as Sapium japonicum (Euphorbiaceae), Euphorbia joldini(Euphorbiaceae), Ilex macropoda (Aquifoliaceae), Styrax japonica (Styracaceae), Carex sideros ticta (Cyperaceae) and Asarum maculatum (Aristolochiaceae) were naturalized into this region, while the 17 taxa such as Abies neprolepis(Pinaceae), Pinus pumila(Pinaceae), Thuja koraiensis(Cupressaceae), Allium senescens(Liliaceae), Lilium distichum(Liliaceae), Saxifraga punctata(Saxfragaceae), Rosa marretii(Rosaceae), Bupleurum euphorbioides(Umbelliferae), Androsace cortusaefolia (Primulaceae), Peducularis mandshurica(Scrophulariaceae) and Leontopodium coreanum (Compositae) were distrivuted to this region. The colonizing weedy species such as Ixris repens (Compositae) were distributed to this region. The colonizing weedy species losa(Labiatae) and Rosa rugosa(Rosaceae) were naturalized into ca. 900m at sea level mainly due to the sand soil from the seashore. Mountain roadbed is susceptible than other areas to the slippery road problems, due largely to snow and rain, particularly during winter. Sand soils from seashore are utilized to minimize this slip in traffic operation.

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Prosobranch Family Velutinidae (Gastropoda) in Cold and Temperate Waters of the Northern Hemisphere: History, Biogeography, Evolution and Chorology

  • Gulbin, V.V.
    • Ocean Science Journal
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    • v.40 no.1
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    • pp.45-54
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    • 2005
  • The history of investigation of the family Velutinidae from 1776 to the present day is briefly described in the last part of this review; the contemporary system of the family, consisting of 39 species and subspecies, belonging to 17 genera and subgenera, and three subfamilies, is presented here. A biogeographic analysis of the fauna has been made, the history of origin and development of the family, vertical distribution and relation to substrates have been described in this work.