• BMB Reports
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• 제52권7호
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• pp.451-456
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• 2019

NDRG1 has been reported to exert pivotal roles in tumor progression and metastasis via Wnt/${\beta}$-catenin signaling pathway. However, little is known about the role of NDRG3 in hepatocarcinogenesis despite its classification in the same subfamily of NDRG1. The present study was aimed to characterize the expression pattern and understand the biological roles of NDRG3 in hepatocarcinogenesis, as a means to exploit its therapeutic potential. It was observed that NDRG3 was up-regulated in HCC tissues and higher NDRG3 expression was associated with significantly shorter overall survival. Furthermore, a lower level of NDRG3 exhibited marked positive correlation with metastasis-free survival. In vitro and in vivo experiments revealed that knock-down of NDRG3 inhibits HCC metastasis and angiogenesis. We further demonstrated that activation of WNT/${\beta}$-catenin signaling and enhanced CSC-like properties were responsible for NDRG3-mediated promoting effect on HCC. In conclusion, the principal findings demonstrated that high NDRG3 expression facilitates HCC metastasis via regulating the turnover of ${\beta}$-catenin, as well as provides a potential therapeutic target for future therapeutic interventions.

• 식물분류학회지
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• 제38권4호
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• pp.371-388
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• 2008

Coryloideae 35집단에 대한 계통 유연관계를 알아보기 위하여 핵 DNA의 waxy 유전자와 엽록체 DNA의 atpB-rbcL intergenic spacer 지역에 대한 염기서열을 분석하였다. Waxy 유전자 분석에서 Coryloideae의 4개 속은 단계통군을 형성하였으며, 개암나무속은 단계통군을 형성하면서 군내군의 가장 기부에 분계조(clade)를 형성하였다. Ostryopsis속은 서어나무속과 새우나무속을 위한 자매군을 형성하였으며, 새우나무속 역시 단계통군을 형성하였다(BS=86, PP=99). AtpB-rbcL 분석에서는 Ostryopsis속이 아과 내에서 가장 기부에 분계조를 형성하였다. 개암나무속은 서어나무속-새우나무속과 Ostryopsis속의 중간에 위치하였고(BS=98, PP=100), 서어나무속은 새우나무속 분류군들과 함께 높은 지지도(BS=100, PP=100)를 가지고 하나의 clade를 형성하였다. Waxy 유전자 분석에서 서어나무속의 Carpinus절은 단계통을 형성하였지만 Distegocarpus절은 병계원군(paraphyletic group)으로 나타났다. 개암나무속의 경우는 2개의 subclade를 형성하였지만 속내 절 또는 아절 등의 분류계급과는 일치하지 않았다. AtpB-rbcL 분석에서는 대부분의 분류군들이 각각의 clade내에서 polytomy를 형성하여 차이를 보였다. 이상의 결과에서 두 유전자의 계통분석 결과가 뚜렷하게 불일치하였고, 특히 Ostryopsis속의 위치와 새우나무속의 단계통 여부, 그리고 서어나무속과 새우나무속의 유연관계가 가장 큰 차이를 보였다. 따라서 이들에 대한 추가적인 연구가 필요한 것으로 판단된다.

• Plant Breeding and Biotechnology
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• 제5권2호
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• pp.123-133
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• 2017

AP2/EREBP gene family consists of transcription factor genes with a conserved AP2 DNA-binding domain and is involved in various biological processes. AP2/EREBP gene families were identified through genome-wide searches in five Cucurbitaceae species including cucumber, wild cucumber, melon, watermelon, and bitter gourd, which consisted of more than 100 genes in each of the five species. The gene families were further divided into five groups including four subfamilies (ERF, DREB, AP2 and RAV) and a soloist group. Among the subfamilies, DREB subfamily which is known to be related to abiotic stress response was more analyzed and a total of 25 genes were identified as Cucurbitaceae homologues of Arabidopsis CBF/DREB1 genes which are important for abiotic stress-response and tolerance. In silico expression profiling using RNA-Seq data revealed diverse expression patterns of cucumber AP2/EREBP genes. AP2/EREBP gene families identified in this study will be valuable for understanding the stress response mechanism as well as facilitating molecular breeding in Cucurbitaceae crops.