• 미생물학회지
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• 제28권4호
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• pp.311-317
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• 1990

Intraspecific and interspecific protoplast fusions in/between C. thermocellum and C. thermohydrosulfuricum were studied. Protoplast fusions were well induced in 30-40% PEG solution, however, their fusion frequencies were low level of 1.2*10$^{-7}$ for intraspecific fusion of C. thermocellum, $6.7*10^{-7}$ for C. thermohydrosulfuricum, and 4.2*10$^{-7}$ for interspecific fusion between above two Clostridia, respectively. Most fusants were unstable and segregated after 3 subcultures. Relatively stable intraspecific C. thermocellum fusant FTT17, intraspecific C. thermohydrosulfurecum fusant FSS22 and interspecific fusant FTS3, which were stable after several subcultures, were selected and properties of fusants were further investigated, Phenotypes of the fusants were similar with wild types mostly in cellular morphology, carbon source assimilation and enzyme activities. However they were differed in assimilation of pyruvic acid and sorbitol as carbon source. The DNA contents of fusants were slightly increased compared with wild types. Ethanol production by intraspecific and/or interspecific fusants was not increased, however, acetic acid production as byproduct was decreased or not detected, which indicates that industrial thermophilic anaerobes can be improved by means of protoplast fusion of two strains.

• 자연과학논문집
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• 제8권2호
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• pp.147-152
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• 1996

딸기의 3품종을 엽과 엽병으로 배양한 결과 2,4-D + BA 처리에서는 치밀한 캘러스가 유기되었고 이 캘러스로부터 다수의 신초가 분화되었으며 NAA + BA 처리에서는 부숴지기 쉬운 캘러스가 유기되었다. '수홍' 엽조직을 NAA나 2,4-D가 첨가된 배지에서 8개월간 배양하면 212번 primer에서 생산된 RAPD band가 모본 band와는 상이하게 나타났다. '보교'와 '여봉'의 엽에서 유기된 캘러스를 2,4-D가 첨가된 액체배지에 계대배양회수를 달리하여 배양하였을 때 12회 계대배양에서 모본과 상이한 RAPD band가 출현되었다.

• Membrane and Water Treatment
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• 제10권3호
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• pp.213-221
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• 2019

Anaerobic digestion (AD) has been widely used to valorize food waste (FW) because of its ability to convert organic carbon into $CH_4$ and $CO_2$. Korean FW has a high content of fruits and vegetables, and efficient hydrolysis of less biodegradable fibers is critical for its complete stabilization by AD. This study examined the digestates from different anaerobic digesters, namely Rs, Rr, and Rm, as the inocula for the AD of vegetable waste (VW) and cellulose (CL): Rs inoculated with anaerobic sludge from an AD plant, Rr inoculated with rumen fluid, and Rm inoculated with anaerobic sludge and augmented with rumen fluid. A total of six conditions ($3\;inocula{\times}2\;substrates$) were tested in serial subcultures. Biogas yield was higher in the runs inoculated with Rm than in the other runs for both VW (up to 1.10 L/g VS added) and CL (up to 1.05 L/g VS added), and so was biogas production rate. The inocula had different microbial community structures, and both substrate type and inoculum source had a significant effect on the formation and development of microbial community structures in the subcultures. The overall results suggest that the bioaugmentation with rumen microbial consortium has good potential to enhance the anaerobic biodegradability of VW, and thereby can help more efficiently digest high fiber-content Korean FW.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1995년도 식물학심포지움 식물로부터 유용 2차대사산물의 생산 PRODUCTION OF USEFUL SECONDARY METABOLITES FROM PLANTS
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• pp.67-78
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• 1995

Monoclonal antibodies (MoAbs) are a highly diversified class of proteins with major research and commercial applications such as diagnostics and therapeutics. Currently, the dominant method for producing MoAbs is through the hybridoma technique. However, this technique is slow, tedious, labor intensive, and expensive. The production of MoAbs in cultured transgenic plant cells can offer some advantages over that in the over that in the mammalian systems. The media to cultivate plant cells are well defined and inexpensive. Contamination by bacteria or fungi is easily monitored in plant tissue cultures. Furthermore, these contaminants are usually not potent pathogens to human beings. In our interdisciplinary research efforts, heavy chain monoclonal antibody (HC MAb) was inserted into Ti plasmid vector and transferred into A. tumefaciens for the transformation in tobacco cells. It was found that 76% of the transformants produced HC MAb. The presence of HC MAb in the cell membrane fraction indicated that the signal peptide was functional and efficient. The change of the HC MAb concentration during a batch culture followed a similar trend as dry cell concentration, indicating that the production of HC MAb was growth related. The long-term repeated subcultures of 11 cell lines showed that there was no obvious trend of neither the decrease nor the increase of the productivity with the repeated subcultures.

• International Journal of Costume and Fashion
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• 제14권1호
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• pp.47-62
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• 2014

People today belong to many different subcultures and have diverse interests. Their job no longer defines who they are, while their pastimes can be a bigger reflection of their inner self. This article examines swing dancers in Korea, and focuses on their self-identity and how they express this through clothes. Based on in-depth interviews, observations and photographs of swing dancers with different day jobs, the study results find that most of the subjects intentionally incorporate swing dance cues in their daily work wardrobe, and that this makes them feel more comfortable, honest and satisfied with their job and identity, both as a swing dancer and working professional.

• 복식
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• 제63권4호
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• pp.118-131
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• 2013

The purpose of this study is to take a look at the fashion frame of Korean women in their twenties and thirties to sort the actual fashion image and the ideal fashion image according to the fashion frame of Korean women in their twenties and thirties, and also to find out the standards and features that divide such a classification. For this study, we used the Q method, which is valued as an effective way to assess subjectivity. This helps to objectively classify the perception the fashion images of and the response to them as well. The analyzed materials were divided into two actual fashion frames and two ideal fashion frames, and classified them into 12 fashion image types in total, that is, six actual fashion images and six ideal fashion images, and we named each type of the fashion images and analyzed the features of each fashion image type through the in-depth Q workshop in which 14 professionals participated. The results of this study are as follows: First, the actual fashion frames of Korean women in their twenties and thirties was largely divided into 'Fashion Gold Girl', the fashion frame of mainstream and 'Indi-idol', the fashion frame of subcultures, and this was further divided into six fashion image types: 'Basic Casual', 'Vintage Performer', 'Easy Chic', 'Ladies' Look', 'City Office Girl' and 'Club Mania'. Second, the ideal fashion frame of Korean women in their twenties and thirties was divided into 'Urban Refinement', the fashion frame of the mainstream and 'Mismatched Style', the fashion frame of subcultures. It was also divided into six fashion image types: Power Fashion', 'Fashion Conservative', 'Semi-culture', 'Fashion Otaku', 'Sweet Darling' and 'Fashion Panic'. Third, The characteristics of the fashion images' colors are recognizable according to the type of fashion images.

• Journal of Plant Biotechnology
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• 제5권3호
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• pp.169-172
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• 2003

Somatic embryogenesis was initiated from in vitro grown leaf explants of rose following an induction period of four weeks on MS basal medium supplemented with auxin and several subcultures on MS medium with cytokinin. '4th of July' showed the highest regeneration frequencies on 1 mg/L NAA followed by culture on medium with 4 mg/L zeatin. The embryogenic callus was propagated on MS medium with NAA, zeatin and $GA_3$. Germination of somatic embryos was achieved on MS medium with 1 mg/L BA. Somatic embryo derived plantlets were hardened and successfully transferred to the greenhouse.

• 디자인학연구
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• 제12권3호
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• pp.93-103
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• 1999

This study is investigated the formative characteristics of Rap Music and Hiphop fashion, and I try to comprehend about the fashion mind and fashion culture of teenager 1. Teenager's Hiphop Fashion is subcultures, the fashion culture of teenager reflects their unique identity. 2. The formative characters of Rap Music and Hiphop Fashion are the incompatibility ignored their body size and the popularity caused street fashion imitation, the unbalance ignored a principle of fashion design. 3. Hiphop Fashion is consist of jump-style jacket, hood -shirt, stripe T-shirt and drum pants, baggy pants, snowboard pants and so on. 4. The characters of Hiphop Fashion is the breaking of rule and the destruction. 5. They select something which they like, even though the price is high, so they is tendency to editing coordinate 6. Fashion business for teenager, it needs an ideal conversion not for 'company' but for 'individual' As this results, fashion culture of teenager came out to the surface of water, because of Hiphop Fashion.

• Mycobiology
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• 제38권4호
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• pp.282-285
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• 2010

Cordyceps cardinalis successfully produced its fruiting bodies from multi-ascospore isolates. However, subcultures of multiascospore isolates could not produce fruiting bodies after few generations. Fruiting body production also differed from sector to sector of the same isolate. Single ascospore isolates were then co-inoculated in combinations of two to observe the fruiting characteristics. Combinations of certain isolates produced perithecial stromata formation, whereas other combinations did not produce any fruiting bodies. These results show that C. cardinalis is a heterothallic fungus, requiring two isolates of opposite mating types for fruiting body production. It was also shown that single ascospore isolates are hermaphrodites.

• 복식
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• 제50권6호
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• pp.155-162
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• 2000

Classifying the diverse and complex clothing category of nationality subcultures in China is not a simple task. However, summing up, it can be largely classified that clothing of the northern area is long trousers and short skirt. Among the clothing of Nationality in China, especially, Pao(robe: 袍) has its diversity and colorfulness. whose elements forming its each characteristic feature has enough value to be investigated fully in the historical point of view. The conclusions of this study reveals that Pao in nationality subculture in China can possibly be divided into the four types as Qipao(旗袍) in Dongpei area, Mongopao(蒙古袍) in Inner Mongolia, Qiapan in Xinjiang area, and Zangpao in Tibet area. The modes of Qipao and Mongopao are mainly similar in that they have diagonally on the right, stand collar in composition, but Qipao shows its diversity in collar and slashes, and Mongopao also shows diversity in its colare and waistband. Since western culture flowed into China along with its open door and reform policy, the splendid color and distinctive pattern, decoration, diversified method of compositions and clothing categories of Nationality cultures are disappearing in its their originality and nationality.