• Parasites, Hosts and Diseases
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• 제51권6호
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• pp.637-644
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• 2013

This study aimed to investigate the antibody responses in mice immunized with Gnathostoma spinigerum crude antigen (GsAg) incorporated with the combined adjuvant, a synthetic oligonucleotide containing unmethylated CpG motif (CpG ODN 1826) and a stable water in oil emulsion (Montanide ISA720). Mice immunized with GsAg and combined adjuvant produced all antibody classes and subclasses to GsAg except IgA. IgG2a/2b/3 but not IgG1 subclasses were enhanced by immunization with CpG ODN 1826 when compared with the control groups immunized with non-CpG ODN and Montanide ISA or only with Montanide ISA, suggesting a biased induction of a Th1-type response by CpG ODN. After challenge infection with live G. spinigerum larvae, the levels of IgG2a/2b/3 antibody subclasses decreased immediately and continuously, while the IgG1 subclass remained at high levels. This also corresponded to a continuous decrease of the IgG2a/IgG1 ratio after infection. Only IgM and IgG1 antibodies, but not IgG2a/2b/3, were significantly produced in adjuvant control groups after infection. These findings suggest that G. spinigerum infection potently induces a Th2-type biased response.

• Parasites, Hosts and Diseases
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• 제51권6호
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• pp.763-766
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• 2013

A synthetic peptide was prepared based on the antigenic region of Paragonimus westermani pre-procathepsin L, and its applicability for immunodiagnosis for human paragonimiasis (due to Paragonimus heterotremus) was tested using an ELISA to detect IgG4 antibodies in the sera of patients. Sera from other helminthiases, tuberculosis, and healthy volunteers were used as the references. This peptide-based assay system gave sensitivity, specificity, accuracy, and positive and negative predictive values of 100%, 94.6%, 96.2%, 100%, and 88.9%, respectively. Cross reactivity was frequently seen against the sera of fascioliasis (75%) and hookworm infections (50%). Since differential diagnosis between paragonimiasis and fascioliasis can be easily done by clinical presentation and fascioliasis serology, this cross reaction is not a serious problem. Sera from patients with other parasitoses (0-25%) rarely responded to this synthetic antigen. This synthetic peptide antigen seems to be useful for development of a standardized diagnostic system for paragonimiasis.

• 한국패류학회지
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• 제26권4호
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• pp.311-316
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• 2010

Spermatid differentiations during spermiogenesis and mature sperm ultrastructure in male Crassostrea nipponica were investigated by transmission electron microscope observations. The morphology of the spermatozoon of this species has a primitive type and is similar to those of other bivalves. Mature spermatozoa consist of broad, cap-shaped acrosomal vesicle and an axial rod in subacrosomal materials on an oval nucleus showing deeply invaginated anteriorly, two triplet substructure centrioles surrounded by four spherical mitochondria, and satelite fibres, which appear near the distal centriole. The acrosomal vesicle of spermatozoa of C. nipponica resemble to those of other investigated ostreids. Especially, two transverse bands (stripes) appear at the anterior region of the acrosomal vesicle, unlikely 2-3 transverse bands (stripes) in C. gigas. It is assumed that differences in this acrosomal substructure are associated with the inability of fertilization between the genus Crassostrea and other genus species in Ostreidae. Therefore, we can use sperm morphology in the resolution of taxonomic relationships within the Ostreidea. The sperm is approximately $48-50{\mu}m$ in length including an oval sperm nucleus (about $1.0{\mu}m$ in length and $1.41{\mu}m$ in width), an acrosome (about $0.48{\mu}m$ in length and 0.30 in width) and tail flagellum ($46-48{\mu}m$). The axoneme of the sperm tail flagellum consists of nine pairs of microtubules at the periphery and a pair at the center. The axoneme of the sperm tail shows a 9 + 2 structure. These morphological charateristics of acrosomal vesicle belong to the family Ostreidae in the subclass Pteriomorphia.

• 한국양식학회지
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• 제15권1호
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• pp.7-13
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• 2002

Since 1993, the White Spot Syndrome Virus (WSSV) disease occurred in China among cultured shrimps resulting in mass mortality. Epizootiological surveys undertaken during the outbreak period of 1993-1994 indicated that all stages of Penaeus chinensis, P. japonicus and P. monodon were infected. Consequent to the transport of contaminated shrimp seedlings and seawater, the disease spread all over the farms of China. The disease was more rapidly transmitted at temperatures above $25^{\circ}C$. Challenge experiments showed the causative agent was highly virulent. White spots appeared on the carapace of both span-taneous and experimentally infected shrimps. Moribund shrimps contained turbid hemolymph, hypertrophied Iymphoid organ and a necrotic mid-gut gland. Electron microscopy showed the presence of viral particles in the gills, stomach, lymphoid organ, and epidermal tissue of the infected shrimp. The visions were slightly ovoid with an envelope and averaged 350 $\times$ 150 nm; nucleocapsids measured 375 $\times$ 157 nm. With discontinuous sucrose gradient of 35, 50 and 60% (w/v), the virus was separated from hemolymph of the infected shrimp. The estimated molecular weight of genomic DNA was 237 Kb with EcoR I, 247 Kb with Hind III and 241kb with Pst I. A total of 9 hybridoma colones secreting monoclonal antibodies (MAbs) were produced from mouse myeloma and spleen cells immunized with WSSV. The immunofluorescence assay of gill tissue showed that the MAbs reacted with diseased but not with healthy shrimp. The MAbs belonged to IgGl, IgG2b subclass and IgM class, all with kappa light Immune-electron-microscopy with colloidal gold marker showed the presence of 5 MAbs epitopes on the envelope and one on the capsid of the virus. Baculoviral mid-gut gland necrosis showed the specificity of the MAbs produced. For diagnosis 5 different methods were selected. Using Kimura primers for PCR, or MAbs for immunoblot, ELISA or FAT method, in situ hybridization was carried out to show the gene. All these methods detected WSSV in the organ samples of the diseased shrimp but not in healthy one.

• 정보처리학회논문지C
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• 제11C권2호
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• pp.253-262
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• 2004

본 논문에서 다중 공유 링크들을 가진 스위치를 위한 세션할당 알고리즘을 제안한다. 제안 알고리즘은 서비스 클래스들에게 사전에 예약된 대역폭을 보장하고, 동일한 서비스 클래스에 속한 세션들에게는 서로 다른 공유 링크를 통해 전송되어도 가능한 비슷한 지연을 제공하고자 한다. 이러한 QoS를 제공하기 위해 다중 공유 링크를 위한 새로운 스케줄링 모델을 정의하고, 이를 기반으로 새로운 세션의 연결 설정 시 이를 어떤 공유 링크에 할당할 것인지를 결정하는 경험적 세션할당 알고리즘을 제안한다. 제안된 알고리즘은 새로운 세션이 소속된 서비스 클래스의 각 링크에 할당된 세션들의 예측된 지연들 중 가장 작은 예측 지연을 가진 링크에게 새로운 세션을 할당한다. 모의실험을 통해 제안 알고리즘을 채택한 스위치가 다른 세션할당 알고리즘을 채택한 스위치에 비해 서비스 클래스들에게 보다 공정한 대역폭을 할당하고 높은 패킷 처리율을 제공하며 예약된 대역폭을 보다 확실히 제공한다는 것을 확인할 수 있었다. 또한 동일한 서비스 클래스의 세션들에게 보다 비슷한 서비스 지연을 제공한다는 것도 확인했다.

• 한국환경복원기술학회지
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• 제4권2호
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• pp.11-25
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• 2001

A wetland is an ecosystem which is the most useful and highly-energetic transition area. This study has been carried out to classify and identify the various types of wetlands in Korea. The main objective of this study are 1) defining and classifying of wetlands, and 2) identifying the wetlands characteristics, and 3) studying cases on the natural wetlands such as Han river, DMZ(Demillitarized Zone), Upo wetland and Yong(Dragon) wetland. The results as follows： 1) Development of the indices for identifying and classifying wetlands in encompassing in such as Ramsar Conference, US NWI(National Wetlands Inventory), Hydrogeomorphic system. 2) Development on the classifying method on the wetlands in the level of supersystem, system, subsystem, class and subclass. The systems include Palustrine and Riverine, and the subsystems are Seasonal, Permanent(Palustrine) and Impersistent, Lower perennial, Impersistent (Riverine). 3) Finally, we find out Young wetland is Palustrine/Permanent/Slope/Persistent, and Upo wetland consists of various types of wetlands, those are, Palustrine/Permanent/Depression/Forest Deciduous, Palustrine/Permanent/Depression/Shrub Deciduous, Palustrine/Permanent/Depression/Persistent, Palustrine /Permanent/Depression/Hydrophytes, and Lacustrine/Permanent/Openwater/Hydrophytes. The taxonomy of this study stems from identifying and classifying wetlands with indices mainly based on hydrologic features and substrates. So, it is needed that consequent studies are to be performed with various viewpoints. And the studying cases were limited because of the restricted entrance into the DMZ, And, we selected only 10 crucial sites in Han river as the subject for wetlands regulation and creation. And, for advanced studies, drawing up inventory and mapping are necessary.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제16권4호
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• pp.482-486
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• 2010

최근 모바일기기에 탑재된 가속도 센서가 제스처기반 모바일 사용자 인터페이스에 활용됨에 따라 동적시간정합(Dynamic Time Warping, DTW)기반 인식기에 대한 연구가 활발하다. DTW는 학습샘플을 매칭 템플릿으로 사용하기 때문에 별도의 학습과정이 없다. 하지만 인식시 입력 데이터를 모든 템플릿과 비교해야하기 때문에 계산복잡도로 인하여 모바일환경에 적용하기 어렵다. 본 논문에서는 이러한 문제를 해결하기 위해 지역화된 소수의 템플릿을 사용하는 DTW기반 제스쳐 인식기를 제안한다. 지역화된 템플릿은 k-평균 클러스터링(k-means clustering)알고리즘을 사용하여 학습 제스처 셋의 유사한 패턴들을 k개의 그룹으로 묶고, 각 그룹의 중심(centroid)에 가까운 패턴을 DTW인식기의 템플릿으로 선택한다. 이러한 방법으로 템플릿수를 줄여 인식속도를 향상하고, 템플릿의 다양성을 유지하여 인식성능저하를 최소화한다. 실험 결과 제안하는 방법이 학습 템플릿을 전부 사용하는 DTW보다 약 5배 빠른 인식속도를 보였으며, 템플릿을 임의로 선택한 경우보다 안정적인 성능을 보임을 확인했다.

• 한국정보처리학회논문지
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• 제7권8호
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• pp.2298-2309
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• 2000

본 논문에서는 객체지향 데이터베이스의 중포속성에 대한 색인기법으로 다차원 색인구조를 이용하는 다차원 중포속성 색인기법은 MD-NAI를 제안한다. 중포석성에 대한 기존의 색인기법들은 중포된 객체에 대한 기존의 색인기법들은 B+-tree와 같은 일차원 색인구조를 이용함으로써, 클래스 계층과 중포속성이 포함된 복합 형태의 질의들에 대한 처리를 잘 지원하지 못한다. MD-NAI에서는 객체지향 데이터베이스의 클래스 계층에 대한 색인기법인 이차원 클래스 계층 색인기법(2D-CHI)을 다차원으로 확장한다. 2D-CHI는 키 속성 도메인과 클래스 식별자 도메인으로 구성된 이차원 도메인 공간상에서 객체들의 클러스터링을 다루는 색인기법이다. 본 논문의 MD-NAI에서는 색인된 중포속성을 표현하는 경로상의 각 클래스 계층마다 하나의 클래스 식별자 도메인을 할당하여 구성된 다차원 도메인 공간상에서 색인 엔트리들의 클러스터링을 다룬다. 따라서, MD-NAI에서는 기존의 색인기법에서 지원하기 어려운 질의의 대상 범위 클래스 계층상의 임의의 클래스들로 제한되거나, 질의에 포함된 복합속성들의 도메인이 클래스 계층상의 임의의 클래스들로 제한되는 경우에도 잘 지원할 수 있다.

• 한국통신학회논문지
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• 제27권8C호
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• pp.758-770
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• 2002

본 논문은 Diffserv 방식의 Assured Service에 대한 QoS를 보장하고 서비스 수용 용량을 최대화 시킬 수 있는 자원 할당 방안을 제안하고, RIO 및 Adaptive RIO 버퍼 관리 방식을 적용하여 성능을 분석한다. Adaptive RIO 방식은 네트워크 토폴로지와 Assured Service 서브 클래스별로 할당되는 대역폭의 비율에 따라 결정되는 버퍼 크기를 기준으로 일정 시간 구간마다 도착 가능한 In-profile 패킷과 전체 패킷의 최대량을 고려하여 RIO 방식의 변수 값들을 재설정함으로써 접속제어가 수행된 상황 하에서 허용된 In-profile 패킷에 대한 Early Random Drop을 방지할 수 있다. 시뮬레이션 결과는 제안하는 자원 할당 방안 및 Adaptive RIO 방식을 적용하여 Assured Service 에 대한 QoS를 보장할 수 있고 서비스 수용 용량을 최대화 시킬 수 있음을 보인다.

• 한국어류학회지
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• 제21권sup1호
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• pp.73-73
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• 2009

The modern cartilaginous fishes, class Chondrichthyes, are small group of Pisces, and comprisingmore 1,000 described valid species, and including approximately 190 genera, 55 families, and 15 orders. The Chondrichthyes consists of two subclasses, the Holocephali (chimeras) and the Elasmobranchii(sharks and rays). The dominant subclass Elasmobranchii includes sharks (Selachii) and rays (Batoidea), the former is more diverse than the latter in higher taxa (order, family, genus) but has fewer species. The Batoidea, batoids or rays, has long been recognized as monophyletic, however, the interrelationships of the groups are problematic and uncertain. In order to review biodiversity and systematics of batoids, valid species name and supraspecific taxa from the published materials are studied. Batoids (including sawfishes, Pristiformes; electric rays, Torpediniformes; stingrays, Myliobatiformes; skates, Rajiformes; wedgefishes, Rhiniformes; and guitarfishes, Rhinobatiformes) is speciose and worldwide with about 580 species in 4 to 6 orders, 17 to 19 families, and about 75 genera. In East Asian waters, the previous records of the Batoidea reveal about 170 species belonging to 41 genera, 18 families, 6 orders. The largest group of batoid fauna in the area is order Myliobatiformes comprising about 80 species, 16 genera of 7 families. The predominant families are Dasyatidae (whiptail stingrays: 46 spp. of 6 genera), Arhynchobatidae (softnose skates: 26 spp. of 3 genera), and Rajidae (skates: 21 spp. of 6 genera). The predominant genera are Bathyraja (softnoseskates: 20 spp.), Himantura (whiprays:19spp.), and Dasyatis (fantailstingrays: 18 spp.). There are 23 species in the Yellow Sea, 70 species in the East China Sea, 82 species in the South China Sea, and 58 species in the North Pacific coast of Japan. Batoid fauna from the East Sea (Sea of Japan) is the poorest of the five regions, consisting of 14 species. Korean waters include only 28 species, 16 genera, 10 families and 5 orders of batoids. Two species is torpediniforms, 4 rhinobatiforms, arhynchobatiforms, 11 rajiforms and 10 myliobatiforms. By the earlier works, the systematics and a check list of the valid species of batoids will be presented.