• Title/Summary/Keyword: sub assembly

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Structural Insights and Mechanistic Understanding of Iron-Molybdenum Cofactor Biosynthesis by NifB in Nitrogenase Assembly Process

  • Wonchull Kang
    • Molecules and Cells
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    • v.46 no.12
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    • pp.736-742
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    • 2023
  • NifB, a radical S-adenosylmethionine (SAM) enzyme, is pivotal in the biosynthesis of the iron-molybdenum cofactor (FeMo-co), commonly referred to as the M-cluster. This cofactor, located within the active site of nitrogenase, is essential for the conversion of dinitrogen (N2) to NH3. Recognized as the most intricate metallocluster in nature, FeMo-co biosynthesis involves multiple proteins and a sequence of steps. Of particular significance, NifB directs the fusion of two [Fe4S4] clusters to assemble the 8Fe core, while also incorporating an interstitial carbide. Although NifB has been extensively studied, its molecular mechanisms remain elusive. In this review, we explore recent structural analyses of NifB and provide a comprehensive overview of the established catalytic mechanisms. We propose prospective directions for future research, emphasizing the relevance to biochemistry, agriculture, and environmental science. The goal of this review is to lay a solid foundation for future endeavors aimed at elucidating the atomic details of FeMo-co biosynthesis.

Design of a BLDC Servo Motor Control System for the Auto Process of Assembly and Supply (자동 조립 및 공급을 위한 BLDC 서보 전동기 제어시스템 설계)

  • Sim, Dong-Seok;Choi, Jung-Keyng
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.16 no.5
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    • pp.1095-1101
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    • 2012
  • This paper presents a design of a BLDC servo motor control system for the auto process of assembly and supply using DSP(Digital Signal Processor) controller and IGBT driver. The assembly and supply auto processing system needs torque, speed, position control of servo motor for variable action. This paper implements those servo control with vector control and space vector PWM(Pulse Width Modulation) technique. As CPU of controller, TMS320F240 DSP was adopted because it has PWM waveform generator, A/D converter, SPI(Serial Peripheral Interface) port and many input/output port etc. This control system consists of 3-level hierarchy structure that main host PC manages three sub DSP system which transfer downward command and are monitoring the states of end servo controllers. Each sub DSP system operates eight BLDC servo controllers which control BLDC motor using DSP and IPM. Between host system and sub DSP communicate with RS-422, between main processor and controller communicate with SPI port.

Non-uniform virtual material modeling on contact interface of assembly structure with bolted joints

  • Cao, Jianbin;Zhang, Zhousuo;Yang, Wenzhan;Guo, Yanfei
    • Structural Engineering and Mechanics
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    • v.72 no.5
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    • pp.557-568
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    • 2019
  • Accurate modeling of contact interface in bolted joints is crucial in predicting the dynamic behavior for bolted assemblies under external load. This paper presents a contact pressure distribution based non-uniform virtual material method to describe the joint interface of assembly structure, which is connected by sparsely distributed multi-bolts. Firstly, the contact pressure distribution of bolted joints is obtained by the nonlinear static analysis in the finite element software ANSYS. The contact surface around bolt hole is divided into several sub-layers, and contact pressure in each sub-layer is thought to be evenly. Then, considering multi-asperity contact at the micro perspective, the relationship between contact pressure and interfacial virtual material parameters for each sub-layer is established by using the fractal contact theory. Finally, an experimental platform for the dynamic characteristics testing of a beam lap structure with double-bolted joint is constructed to validate the efficiency of proposed method. It is found that the theoretical results are in good agreement with experimental results by impact response in both time- and frequency-domain, and the relative errors of the first four natural frequencies are less than 1%. Furthermore, the presented model is used to examine the effect of rough contact surface on dynamic characteristics of bolted joint.

Thickness Dependence of Size and Arrangement in Anodic TiO2 Nanotubes

  • Kim, Sun-Mi;Lee, Byung-Gun;Choi, Jin-Sub
    • Bulletin of the Korean Chemical Society
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    • v.32 no.10
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    • pp.3730-3734
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    • 2011
  • The degree of self-assembly and the size variation of nanotubular structures in anodic titanium oxide prepared by the anodization of titanium in ethylene glycol containing 0.25 wt % $NH_4F$ at 40 V were investigated as a function of anodization time. We found that the degree of self-assembly and the size of the nanotubes were strongly dependent on thickness deviation and thus indirectly on anodization time, as the thickness deviation was caused by the dissolution of the topmost tubular structures at local areas during long anodization. A large deviation in thickness led to a large deviation in the size and number of nanotubes per unit area. The dissolution primarily occurred at the bottoms of the nanotubes ($D_{bottom}$) in the initial stage of anodization (up to 6 h), which led to the growth of nanotubes. Dissolution at the tops ($D_{top}$) was accompanied by $D_{bottom}$ after the formed structures contacted the electrolyte after 12 h, generating the thickness deviation. After extremely long anodization (here, 70 h), $D_{top}$ was the dominant mode due to increase in pH, meaning that there was insufficient driving force to overcome the size distribution of nanotubes at the bottom. Thus, the nanotube array became disorder in this regime.

Synthesis and characterization of three-dimensional monodispersed NiO/NiCo2O4 via Ni3[Co(CN)6]2 PBA nanocubes (Ni3[Co(CN)6]2 PBA 나노큐브를 통한 단분산된 3차원 구조의 NiO/NiCo2O4 제조 및 특성 평가)

  • Kwag, Sung Hoon;Lee, Young Hun;Kim, Min Seob;Lee, Chul Woo;Kang, Bong Kyun;Yoon, Dae Ho
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.27 no.3
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    • pp.110-114
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    • 2017
  • $NiO/NiCo_2O_4$ nanocubes were successfully synthesized via the calcination process of $Ni_3[Co(CN)_6]_2$ PBAs. The prepared monodispersed $Ni_3[Co(CN)_6]_2$ PBAs were aggregated by 'self-assembly' of the nuclei generated during the synthesis reaction. The self-assembly rate of the particles is affected by the temperature and the amount of surfactant SDBS (sodium dodecylbenzenesulfonate). FESEM analysis shows that monodispersed 200 nm PBA nanocubes are obtained at 0.25 g SDBS and $60^{\circ}C$ temperature. Thermal behavior was confirmed by thermogravimetric-differential thermal analysis (TG-DTA) to determine optimal calcination conditions. Then, field emission scanning electron microscopy (FESEM) and X-ray diffraction (XRD) analyzes were performed to investigate the morphology and crystallinity of the particles precursors and $NiO/NiCo_2O_4$ nanocubes.

APPLICATION OF A GENETIC ALGORITHM FOR THE OPTIMIZATION OF ENRICHMENT ZONING AND GADOLINIA FUEL (UO2/Gd2O3) ROD DESIGNS IN OPR1000s

  • Kwon, Tae-Je;Kim, Jong-Kyung
    • Nuclear Engineering and Technology
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    • v.44 no.3
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    • pp.273-282
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    • 2012
  • A new effective methodology for optimizing the enrichment of low-enriched zones as well as gadolinia fuel ($UO_2/Gd_2O_3$) rod designs in PLUS7 fuel assemblies was developed to minimize the maximum peak power in the core and to maximize the cycle lifetime. An automated link code was developed to integrate the genetic algorithm (GA) and the core design code package of ALPHA/PHOENIX-P/ANC and to generate and evaluate the candidates to be optimized efficiently through the integrated code package. This study introduces an optimization technique for the optimization of gadolinia fuel rod designs in order to effectively reduce the peak powers for a few hot assemblies simultaneously during the cycle. Coupled with the gadolinia optimization, the optimum enrichments were determined using the same automated code package. Applying this technique to the reference core of Ulchin Unit 4 Cycle 11, the gadolinia fuel rods in each hot assembly were optimized to different numbers and positions from their original designs, and the maximum peak power was decreased by 2.5%, while the independent optimization technique showed a decrease of 1.6% for the same fuel assembly. The lower enrichments at the fuel rods adjacent to the corner gap (CG), guide tube (GT), and instrumentation tube (IT) were optimized from the current 4.1, 4.1, 4.1 w/o to 4.65, 4.2, 4.2 w/o. The increase in the cycle lifetime achieved through this methodology was 5 effective full-power days (EFPD) on an ideal equilibrium cycle basis while keeping the peak power as low as 2.3% compared with the original design.

Sequencing for a mixed model assembly line in just-in-time production system (JIT 상황하에서 다품종 조립라인 작업물 투입 순서 결정 방안)

  • Hwang, Hark;Jeong, In-Jae;Lim, Joon-Mook
    • Korean Management Science Review
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    • v.11 no.1
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    • pp.91-106
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    • 1994
  • In mixed model assembly lines, products are assembled seqeuntially that have different combination of options specified by customers. In just in time (JIT) environment, production smoothing becomes an important issue for sub-lines which supply the necessary parts to each workstation of the assembly line. Another important issue is to avoid line stopping caused by work overload in workstations. To find a sequence which minimizes the costs associated with line stoppage and the option parts inventory level, a nonlinear mixed integer programming is formulated. Recognizing the limit of the Branch and Bound technique in large sized problems, a heuristic solution procedure is proposed. The performance of the heuristic is compared with the Branch and Bound technique through randomly generated test problems. The computational results indicate that on the average the heuristic solutions deviate approximately 3.6% from the optimal solutions.

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Self-assembly of Si-containing block copolymers for next-generation nanofabrication

  • Jeong, Yeon-Sik
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.02a
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    • pp.22-23
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    • 2011
  • As device dimensions shrink, it is increasingly important to develop fabrication methods that can create sub-15 nm features of regular or arbitrary geometry in a rapid, parallel, and efficient process. This talk will discuss approaches based on self-assembling hybrid polymers containing Si. The thin films of those materials systems can generate well-ordered periodic arrays of dots or lines. For achieving, long-range ordering, it is helpful to use lithographically-defined templates, which are in general much larger than the length-scale of self-assembled nanostructures. For example, the self-assembly of polymer nanostructures can easily be templated using an array of nanoscale topographical elements that act as guiding templates or surrogates for one of two microdomains. The solvent-vapor-induced tunability of pattern dimension and morphology will be discussed as well. Those material systems can excellently serve for high-precision self-assembly that can provide good resolution, reliability, and controllability and be considered as an option for a future nanomanufacturing technology.

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Assembly processes of moss and lichen community with snow melting at the coastal region of the Barton Peninsula, maritime Antarctic

  • Kim, Seok Cheol;Kim, Jun Seok;Hong, Bo Ram;Hong, Soon Gyu;Kim, Ji Hee;Lee, Kyu Song
    • Journal of Ecology and Environment
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    • v.40 no.1
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    • pp.55-65
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    • 2016
  • Background: In this article, it was analyzed how snow melting affects the assembly of lichen and moss communities in a small area of the coastal region of Barton Peninsula, which is in maritime Antarctic. In the small area, even though there is a huge gap of difference of the environment between the snow-filled area and snow-melt one, the latter did not have distinctive environmental gradients. Results: Depending on the snow melting time, coverage and species diversity of lichens and mosses tend to increase remarkably. For species with significant changes depending on the snow-covered period, there are Andreaea regularis, crustose lichens, Placopsis contortuplicata, Usnea aurantiaco-atra, and snow algae. In this area, the process of vegetation assembly process has shown the directional development in the order of snow algae${\rightarrow}$crustose, lichen sub-formation${\rightarrow}$fruticose lichen, moss cushion sub-formation (Andreaea sociation)${\rightarrow}$fruticose lichen, and moss cushion sub-formation (Usnea sociation), according to the order of snow melting. These directional development stages are shown in gradual change in small area with the snow melting phenomena. However, in the snow-free area, where water is sufficiently supplied, it is expected that moss carpet sub-formation (Sanionia sociation) will be developed. Vegetation development in the small area with the snow melting phenomena, depending on differences of resistance on snow kill and moisture settled by species in according to the time of snow melting, tolerance model to form community is followed. Conclusions: The research results explain the development of vegetation in the Antarctic tundra and its spatial distribution according to the period for growth of lichens and mosses in the summer time by differences of snow melting in the small area. In the future, if research for the community development process in a large scale will be done, it will be helpful to figure out temporal and spatial dynamic of vegetation in the Antarctic tundra where snow and glaciers melt rapidly due to climatic warming.