• 동력기계공학회지
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• 제5권4호
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• pp.44-52
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• 2001

This paper considers a modeling and identification for the MIMO magnetic bearing system. To obtain the nominal plant transfer functions, we have experimented on the frequency response by a closed-loop identification method because the system is unstable essentially. We suggest a method of curve-fitting for obtaining the transfer function from the frequency responses by using the system's modeling structure and two controllers which are different from each other. From the frequency response results, we found the effects of coupling by opposing controllers. And using this effects and the system's modeling structure, we could obtain the transfer functions of which have the same modularized denominators.

• Journal of Plant Biotechnology
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• 제49권1호
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• pp.39-45
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• 2022

Conserved domains are defined as recurring units in molecular evolution and are commonly used to interpret the molecular function and biochemical structure of proteins. Herein, the ADP-glucose pyrophosphorylase (AGPase) amino acid sequences of three species of the Ipomoea genus [Ipomoea trifida, I. triloba, and I. batatas (L.) Lam. (sweetpotato)] were identified to investigate their physicochemical and biochemical characteristics. The molecular weight, isoelectric point, instability index, and grand average of hyropathy markedly differed among the three species. The aliphatic index values of sweetpotato AGPase proteins were higher in the small subunit than in the large subunit. The AGPase proteins from sweetpotato were found to contain an LbH_G1P_AT_C domain in the C-terminal region and various domains (NTP_transferase, ADP_Glucose_PP, or Glyco_tranf_GTA) in the N-terminal region. Conversely, most of its two relatives (I. trifida and I. triloba) were found to only contain the NTP_transferase domain in the N-terminal region. These findings suggested that these conserved domains were species-specific and related to the subunit types of AGPase proteins. The study may enable research on the AGPase-related specific characteristics of sweetpotatoes that do not exist in the other two species, such as starch metabolism and tuberization mechanism.

• 한국에너지공학회:학술대회논문집
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• 한국에너지공학회 1996년도 춘계학술발표회 초록집
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• pp.88-96
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• 1996

The characteristic analysis of fly ash generated from a fired power plant using bunker-C oil has been investigated. Ash size distribution by an optical microscopy with image processing technique, morphological shape by a scanning electron microscope(SEM) and microscope, chemical composition by the inductively coupled plasma emission spectrometry(ICP), and resistivity measurement as a function of temperature and moisture content by the resistivity meter are performed. A study of physical, chemical and electrical characteristics of bunker-C fly ash plays an important role of improving the performance of an electrostatic precipitator and protecting air pollution. The samples of bunker-C fly ash for analysis were collected from the electrostatic precipitator hopper of Ulsan Power Plant Unit 1 and Pusan Power Plant Unit 1. Mass median diameter(MMD) of bunker-C fly ash was measured 12.7${\mu}{\textrm}{m}$, while MMD of fly ash generated from the mixture of bunker-C oil(40%) and domestic anthracitic coal(60%) was 25.7${\mu}{\textrm}{m}$. The morphological structure of bunker-C fly ash consisted of fine particles of non-spherical shape. The primary chemical components of bunker-C fly ash were composed of SiO2(2.36%), Al2O3(4.91%), Fe2O3(14.33%) and C(11.84%). Resistivity of bunker-C fly ash was found to be increased with increasing temperature at the range of 100~15$0^{\circ}C$ and was measured 103~104 ohm-cm.

• 한국안전학회지
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• 제30권3호
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• pp.107-113
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• 2015

The goal of this paper is to investigate structural integrity factors of RMI(reflective metal insulation) to confirm the design requirements in nuclear power plant. Currently, a glass wool insulation is using now, but it will gradually be replaced with the reflective metal insulation maded by stainless steel plates. The main function of an insulation is to minimize a heat loss of vessel and pipes in RCS(reactor coolant system). It has to maintain structural a integrity in nuclear power plant life duration. In this study, the structural integrity analysis was carried out both multi-plate and outer shell plate by using a static analysis and experimental test. First, inner multi-plate has a self support structure for being air space. Because the effect of total static weight in multi-layer plate is low, a plate collapse possibility is not high. Considering optimum thin plate pressing process, it has to pre-check the basic physical properties. Second, the outer segment thickness and stiffener shape are verified by the numerical static analysis, and sample test for both type of panel and cylindrical pipe model.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2004년도 가을 학술발표회 논문집
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• pp.379-386
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• 2004

The optimal seismic retrofitting of NPP(Nuclear Power Plant) cabinet structures that contained class 1 relays were studies in this paper. During earthquake event, the failure modes of relays are not appeared in form of structural failure, but are appeared in form of contact chatter of relay. Therefore, the retrofitting of cabinet has to be aimed to the reducing of the structural response, such as acceleration. In this study, the optimal characteristic values of dampers were searched by μ-GA (micro-Genetic Algorithm) scheme for several installation patterns. To keep accuracy and efficiency of analysis, the structural models of cabinet were considered as a frame structure. The responses of structure were obtained in form of acceleration response spectra derived from the results of nonlinear time history analysis including damping nonlinearity. The fitness function of the optimum procedure was constructed based on the ratio of maximum spectral value and target GERS (General Equipment Ruggedness Spectra). The results show the good improvements of fitness fur adequate retrofitting pattern. Especially, the improvements of fitness were remarkable when the damping exponents are proper.

• 한국발생생물학회지:발생과생식
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• 제26권1호
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• pp.23-36
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• 2022

Pharyngeal pouches, a series of outgrowths of the pharyngeal endoderm, are a key epithelial structure governing facial skeleton development in vertebrates. Pouch formation is achieved through collective cell migration and rearrangement of pouch-forming cells controlled by actin cytoskeleton dynamics. While essential transcription factors and signaling molecules have been identified in pouch formation, regulators of actin cytoskeleton dynamics have not been reported yet in any vertebrates. Cofilin1-like (Cfl1l) is a fish-specific member of the Actin-depolymerizing factor (ADF)/Cofilin family, a critical regulator of actin cytoskeleton dynamics in eukaryotic cells. Here, we report the expression and function of cfl1l in pouch development in zebrafish. We first showed that fish cfl1l might be an ortholog of vertebrate adf, based on phylogenetic analysis of vertebrate adf and cfl genes. During pouch formation, cfl1l was expressed sequentially in the developing pouches but not in the posterior cell mass in which future pouch-forming cells are present. However, pouches, as well as facial cartilages whose development is dependent upon pouch formation, were unaffected by loss-of-function mutations in cfl1l. Although it could not be completely ruled out a possibility of a genetic redundancy of Cfl1l with other Cfls, our results suggest that the cfl1l expression in the developing pouches might be dispensable for regulating actin cytoskeleton dynamics in pouch-forming cells.

• 농촌계획
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• 제14권1호
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• pp.59-71
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• 2008

The landscape commonly refers to the appearance of the land, including its shape, texture, and colours. Among many factors contributing to the landscape, tree and plants factors playa significant role in rural landscape as a colours. Therefore the rural traditional theme village where had been designated under the rural traditional theme village since 2007 in Naegok-ri, Eohang-myeon, Haman-gun has planned to design the tree plant planning for creating a unique rural landscape. The landscape proposal for the new Rural Village in Naegokri, Yeohang-myeon, Haman-gun suggests three main strategies; 1) The riverside area and reservoir area adjoined forest was required to be prepared to offer organization waterside landscape and forest landscape through landscape planting and selecting trees i.e. able to reflect sense of the season that promote high quality values of landscape area in rural. 2) The area adjoined stable was required to strengthen the screen planting and buffer planting with multiple plant layer structure and trees of branches and leaves closeness, shrubs of beauty flowers in order to improve disamenity landscape and odours. 3) The rural traditional theme village adjoined entrance was required to open space such as multipurpose garden, specialized or themed garden which include Landmark factors and prepared to the space with the various theme and visual diversity in order to take charge of function as landmark characteristic in Naegok-ri, Eohang-myeon, Haman-gun, and so on. In conclusion, this winning principal purpose of this study is applied to basic tree plant model for sustainable rural landscape creation in rural areas by selecting beautiful landscape plants and the tree plant planning.

• Journal of Plant Biotechnology
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• 제2권2호
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• pp.79-82
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• 2000

We have isolated a cDNA encoding a calcium-dependent protein kinase (CDPK) in Nicotiana tabacum, which was designated NtCDPK1. Accumulation of the NtCDPK1 mRNA was stimulated by various stimuli, including phytohormones, CaCl$_2$ wounding, fungal elicitors, chitin and methyl jasmonate. The NtCDPK1 gene encodes a functional Ser/Thr protein kinase of which phosphorylation activity is strongly induced by calcium. By analyzing expression of the NtCDPK1-GFP fusion protein and by immunoblotting with antibody which reacts with NtCDPK1, we found that NtCDPK1 is localized in membrane and nucleus in plant cells. Silencing expression of the NtCDPK1 transgene resulted in marked decrease of lateral root development in the transgenic tobacco plants. Yeast two hybrid screening using NtCDPK1 as a bait identified a tobacco homologue of proteasome regulatory subunit 21D7, designated Nt21D7. The 21D7 mRNA has been shown to be predominantly expressed in proliferating tissues in the cell cycledependent manner in carrot. The recombinant NtCDPK1 protein associated with Nt21D7 in vitro, and could phosphorylate the Nt21D7 protein in vitro in the presence of calcium, suggesting that Nt21D7 protein is a natural substrate of NtCDPK1 in tobacco. These results suggest that NtCDPK1 may regulate tell proliferation processes, such as lateral root formation, by regulating specificity and/or activity of proteasome-mediated protein degradation pathway.

• Journal of Ginseng Research
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• 제39권4호
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• pp.406-413
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• 2015

Background: Pathogenesis-related 10 (PR-10) proteins are small, cytosolic proteins with a similar three-dimensional structure. Crystal structures for several PR-10 homologs have similar overall folding patterns, with an unusually large internal cavity that is a binding site for biologically important molecules. Although structural information on PR-10 proteins is substantial, understanding of their biological function remains limited. Here, we showed that one of the PgPR-10 homologs, PgPR-10.3, shares binding properties with flavonoids, kinetin, emodin, deoxycholic acid, and ginsenoside Re (1 of the steroid glycosides). Methods: Gene expression patterns of PgPR-10.3 were analyzed by quantitative real-time PCR. The three-dimensional structure of PgPR-10 proteins was visualized by homology modeling, and docking to retrieve biologically active molecules was performed using AutoDock4 program. Results: Transcript levels of PgPR-10.3 expressed in leaves, stems, and roots of 3-wk-old ginseng plantlets were on average 86-fold lower than those of PgPR-10.2. In mature 2-yr-old ginseng plants, the mRNA of PgPR-10.3 is restricted to leaves. Ginsenoside Re production is especially prominent in leaves of Panax ginseng Meyer, and the binding property of PgPR-10.3 with ginsenoside Re suggests that this protein has an important role in the control of secondary metabolism. Conclusion: Although ginseng PR-10.3 gene is expressed in all organs of 3-wk-old plantlets, its expression is restricted to leaves in mature 2-yr-old ginseng plants. The putative binding property of PgPR-10.3 with Re is intriguing. Further verification of binding affinity with other biologically important molecules in the large hydrophobic cavity of PgPR-10.3 may provide an insight into the biological features of PR-10 proteins.

• 한국과학교육학회지
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• 제22권4호
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• pp.796-805
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• 2002

본 연구는 '식물의 구조와 기능' 단원의 학습 전 아동의 지식구조와 학습과정 및 학습 종료 7개월 후까지의 지식구조의 변화를 아동의 학습성향(유의미학습과 암기학습)과 관련지워 조사한 것이다. 연구단원과 관련된 기존의 지식구조를 알아보기 위하여 5학년 아동들에게 1차 개념도를 그리게 하고, 매 4차시 수업후 각각의 개념도를 그리게 했다. 그리고 지식구조의 지속효과를 알아보기 위하여 단원 학습이 종료된 직후와 3개월, 7개월 후에도 각각의 개념도를 그리게 했다. 학습과정 동안에는 기존의 개념도를 근거로 하여 개념도를 그리게 하였으며, 단원 학습 종료 후에는 기존의 개념도가 제시되지 않은 상태에서 개념도를 그리게 하였다. 아동들이 그린 각각의 개념도는 그 당시의 식물의 구조와 기능에 대한 이해 정도를 나타내었다. 초등학교 5학년 아동들은 '식물의 구조와 기능' 단원의 학습에 들어가기 전에 이와 관련된 여러 가지 개념들로 이루어진 사전 지식구조를 가지고 있으나 그 중 과학적으로 타당한 것은 개념도 상에서 2단계의 위계와 5개의 관계에 불과하였다. 한 단원의 학습이 진행되어감에 따라 아동들의 지식구조는 꾸준히 그리고 점진적으로 증가였다. 그러나 이러한 증가는 학습성향에 따라 차이를 보이며, 유의미 학습자가 암기 학습자보다 더 많이 증가하였다(관계, 연관, 예: p < .01, 위계: p < .05). 지식구조의 변화에 있어서 학습성향간에 다소 차이가 있으나 재구조화는 단원 학습의 초기에 높은 빈도로 일어났으며, 말기에는 전혀 일어나지 않았다. 조정은 유의미 학습자보다 암기 학습자에서 더 많이 일어나고 확장은 이와 반대로 유의미 학습자에서 더 많이 일어났다. 암기 학습자의 개념도 점수는 단원 학습을 마친 후 시간이 경과함에 따라 모든 범주에서 차츰 감소하였다. 그러나 유의미 학습자의 경우 단원 학습 후 3개월보다 7개월에서 오히려 소폭 증가했다. 그러므로 대체로 암기 학습자가 학습과정에서 새로이 구축하고자 하는 더 분화되고 정교한 지식구조가 유의미 학습자보다 불안정한 상태에 있으며, 지식구조의 분화 정도도 유의미 학습자에 비해 낮다고 할 수 있다.