• BMB Reports
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• 제43권1호
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• pp.1-8
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• 2010

The cold shock domain (CSD) is among the most ancient and well conserved nucleic acid binding domains from bacteria to higher animals and plants. The CSD facilitates binding to RNA, ssDNA and dsDNA and most functions attributed to cold shock domain proteins are mediated by this nucleic acid binding activity. In prokaryotes, cold shock domain proteins only contain a single CSD and are termed cold shock proteins (Csps). In animal model systems, various auxiliary domains are present in addition to the CSD and are commonly named Y-box proteins. Similar to animal CSPs, plant CSPs contain auxiliary C-terminal domains in addition to their N-terminal CSD. Cold shock domain proteins have been shown to play important roles in development and stress adaptation in wide variety of organisms. In this review, the structure, function and regulation of plant CSPs are compared and contrasted to the characteristics of bacterial and animal CSPs.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2004년도 ICCAS
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• pp.936-941
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• 2004

An Optimal Feedforward Integral Variable Structure or FIVSC approach for an electrohydraulic position servo control system is presented in this paper. The FIVSC algorithm combines feedforward strategy and integral in the conventional Variable Structure Control (VSC) and calculating the control function to guarantee the existence of a sliding mode. Furthermore, the chattering in the control signal is suppressed by replacing the sign function in the control function with a smoothing function. The simulation results illustrate that the purposed approach gives a significant improvement on the tracking performances when compared with some existing control methods, like the IVSC and MIVSC strategies. Simulation results illustrate that the purposed approach can achieve a zero steady state error for ramp input and has an optimal motion with respect to a quadratic performance index. Moreover, Its can achieve accurate servo tracking in the presence of plant parameter variation and external load disturbances.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1998년도 The 12th Symposium on Plant Biotechnology Vol.12
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• pp.83-96
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• 1998

The plant pigment proteins phytochromes are a molecular light sensor or switch for photomorphogenesis involving a variety of growth and developmental responses of plants to red and far-red wavelength light. Underscoring the photomorphogenesis mediated by phytochromes is the light signal transduction at molecular and cellular levels. For example, a number of genes activated by the phytochrome-mediated signal transduction cascade have been identified and characterized, especially in Arabidopsis thaliana. The light sensor/switch function of phytochromes are based on photochromism of the covalently linked tetrapyrrole chromophore between the two photoreversible forms, Pr and Pfr. The photochromism of phytochromes involves photoisomerization of the tetrapyrrole chromophore. The "photosensor" Pr-form ("switch off" conformation) of phytochromes strongly absorbs 660 nm red light, whereas the "switch on" Pfr-conformation preferentially absorbs 730 nm far-red light. The latter is generally considered to be responsible for eliciting transduction cascades of the red light signal for various responses of plants to red light including positive or negative expression of light-responsive genes in plant nuclei and chloroplasts. In this paper, we discuss the structure-function of phytochromes in plant growth and development, with a few examples of biotechnological implications.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2002년도 심포지엄
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• pp.98-98
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• 2002

In higher dicotyledonous plants, the floral organs are arranged in four different whorls, containing sepals, stamens and carpels. petals, stamens and carpels. The specification of floral organ identity is explained by the ABC model (Weigel and Meyerowitz 1994). expression of an A-function gene specifies sepal formation in whorl 1. the combination of A-and B-function genes specifies the formation of petals in whorl 2, B-and C-function genes spesify stamen formation in whorl 3, and expression of the C-function alone determines the formation of carpels in whorl 1. A-, B-, C-function genes have been isolated from many plant species and most of them belong to the family of MADS-box genes encoding transcription factor. In contrast to the flower of higher dicots, the perianths of genseng plants have three whorls of almost identical petaloid organs. van Tunen et al. (1993) proposed a modified ABC model, exemplified with tulip. In this model, B-function genes are expressed in whorl 1 as well as whorl 2 and 3, theefore the organs of whorl 1 and whorl 2 have the same petaloid structure. They proposed this model with the molphological data of wild type and mutant flowers of tulip, however, there are no molecular data. To date, B-function genes were isolated several grass plants, rice, wheat and maize. However, grass plants have highly derived flowers, without well-developed perianths. To find out how the ABC model has to be modified for the Genseng plants, we have cloned and characterized orthologs of A-, B-, C-function genes from genseng.

• Journal of Plant Biotechnology
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• 제1권1호
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• pp.13-19
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• 1999

The visco-elastic properties of gluten are major determinants of the processing properties of doughs. These visco-elastic properties are strongly influenced by the ratio of monomeric and polymeric proteins and the size distribution of the polymeric proteins, which make up the gluten fraction of the dough. Recent studies have revealed that other features, such as the number of the cysteine residues of the HMW-GS, also play an important role in determining the functional characteristics. To modify the processing properties at molecular level, the relationship between the structure of molecules and dough properties has to be understood. In order to explore the relationships between individual proteins and dough properties, we have developed procedures for incorporating bacterially expressed proteins into doughs, and measuring their functional properties in small-scale equipment. A major problem in investigating the structure/function relationships of individual seed storage proteins is to obtain sufficient amounts of pure polypeptides from the complex families of proteins expressed in the endosperm. Therefore, we have established a simplified model system in which we produce specific protein genes through bacterial expression and test their functional properties in smallscale apparatus after incorporation into base flour. An S poor protein gene has been chosen as a template gene. This template gene has been modified using standard recombinant DNA techniques in order to test the effects of varying the number and position of cysteine residues, and the size of the protein. Doughs have been mixed in small scale apparatus and characterized with respect to their polymeric composition and their functional properties, including dough mixing, extensibility and small scale bating. We conclude that dough characteristics can be manipulated in a predictable manner by altering the cysteine residues and the size of high molecular weight glutenins.

• 전자공학회논문지B
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• 제29B권8호
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• pp.24-34
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• 1992

A process control language is developed using function block configuration, to simplify software development for large scale process control systems, and to implement advanced control algorithms with ease. A function block parser and controller is implemented to be suitable for multi-loop control systems having hierachical structure. On-line change of controller parameter is possible, and inclusion of user defined function block is also possible. By adding plant model block, control performance can be checked in advance. Function blocks of the Smith Predicotor, auto-tuners are implemented to demonstrate usefulness of function block configuration.

• 한국경영공학회지
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• 제23권4호
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• pp.87-103
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• 2018

This paper introduces the method to develop two main types of the fuzzy OEE (Overall Equipment Effectiveness) models via triangular membership function for measuring uncertainty. The fuzzy OEE includes model type 1 and model type 2. The model type 1 is used when the theoretical machine speed only reflects the time loss whereas model type 2 is used when the actual machine speed reflects both time and speed loss. Model type 2 has shown to perform a lower availability rate and a higher performance rate compared to model type 1. In addition, the fuzzy UPH (Unit Per Hour) which is derived from using the fuzzy OEE is presented to satisfy demand uncertainty. The fuzzy UPH can easily measure the fuzzy tact time and cycle time by reciprocating itself. Finally, this study demonstrates the fuzzy OEE models using IVIFS (Interval-Valued Intuitionistic Fuzzy Set) based on the characterization via membership function, non-membership function and hesitant function. For the purpose of analyzing the fuzzy system OEE, the OEE for each machine of plant structure is considered triangular interval-valued intuitionistic fuzzy number. Regardless of plant structure, the validity degree of fuzzy membership function of system OEE decreases when the number of machine with worst value of the validity degree increases. Corresponding examples are presented in this paper for practitioner to understand the applicability and practicability of the proposed fuzzy OEE methods.

• Plant Biotechnology Reports
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• 제5권4호
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• pp.331-344
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• 2011

The well-conserved NBS domain of resistance (R) genes cloned from many plants allows the use of a PCR-based approach to isolate resistance gene analogs (RGAs). In this study, we isolated an RGA (CapRGC) from Capsicum annuum "CM334" using a PCR-based approach. This sequence encodes a protein with very high similarity to Rx genes, the Potato Virus X (PVX) R genes from potato. An evolutionary analysis of the CapRGC gene and its homologs retrieved by an extensive search of a Solanaceae database provided evidence that Rx-like genes (eight ESTs or genes that show very high similarity to Rx) appear to have diverged from R1 [an NBS-LRR R gene against late blight (Phytophthora infestans) from potato]-like genes. Structural comparison of the NBS domains of all the homologs in Solanaceae revealed that one novel motif, 14, is specific to the Rx-like genes, and also indicated that several other novel motifs are characteristic of the R1-like genes. Our results suggest that Rx-like genes are ancient but conserved. Furthermore, the novel conserved motifs can provide a basis for biochemical structural. function analysis and be used for degenerate primer design for the isolation of Rx-like sequences in other plant species. Comparative mapping study revealed that the position of CapRGC is syntenic to the locations of Rx and its homolog genes in the potato and tomato, but cosegregation analysis showed that CapRGC may not be the R gene against PVX in pepper. Our results confirm previous observations that the specificity of R genes is not conserved, while the structure and function of R genes are conserved. It appears that CapRGC may function as a resistance gene to another pathogen, such as the nematode to which the structure of CapRGC is most similar.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2002년도 제9차 국제심포지움 및 추계정기학술발표회
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• pp.15-15
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• 2002

In higher dicotyledonous plants, the floral organs are arranged in four different whorls, containing sepals, petals, stamens and carpels. petals, stamens and carpels. The specification of floral organ identity is explained by the ABC model (Weigel and Meyerowitz 1994). Expression of an A-function gene specifies sepal formation in whorl 1. the combination of A-and B-function genes specifies the formation of petals in whorl 2, B-and C-function genes spesify stamen formation in whorl 3, and expression of the C-function alone determines the formation of carpels in whorl 4. A-. B-, C-function genes have been isolated from many plant species and most of them belong to the family of MADS-box genes encoding transcription factor. In contrast to the flower of higher dicots, the perianths of genseng plants have three whorls of almost identical petaloid organs. van Tunen et al. (1993) proposed a modified ABC model, exemplified with tulip. In this model, B-function genes are expressed in whorl 1 as well as whorl 2 and 3, theefore the organs of whorl 1 and whorl 2 have the same petaloid structure. They proposed this model with the molphological data of wild type and mutant flowers of tulip, however, there are no molecular data.(중략)

• 한국초등과학교육학회지:초등과학교육
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• 제21권1호
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• pp.13-24
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• 2002

This study examined the knowledge structure constructed by children before formal instruction, and successive changes in the structural complexity of knowledge during the learning of the 'Structure and Function of Plants' unit. The researchers let the 5th graders draw the first draft of their concept maps in order to examine the pre-existing knowledge structure concerned with the unit and also four concept maps after completing every fourth lesson. Each concept map drawn by children on the basis of the previous one showed the degree of their current understanding on the structure and function of plants. The results revealed that only two levels of hierarchy and five relationships among the components of the first concept map(relationship, hierarchy, cross link and example) were proven to be valid in terms of conceptual relevance. According to the standard map, there was no change in hierarchy from the 2nd to the 3rd map, and in example from the 2nd to the 4th map. However, the gradual and successive increases of the scores in all components appeared in the children's maps throughout the unit. Knowledge restructuring occurred strongly in the early periods from the 1st to the 6th lesson, and the significant stable changes in tuning and accretion appeared throughout the whole lessons. The results also showed that there were no significant gender differences on the 5th grader's knowledge structuring.