• Applied Chemistry for Engineering
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• v.7 no.2
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• pp.252-260
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• 1996

In this article, we investigate into the structural changes of liposome to design its functional membranes by the synthesis of two types of liposomes, DPPC liposome and DTAB of hydrocarbon substance/DPPC liposome. The changes of membrane structures are evaluated by the CF fluorescent intensity measured above and below the phase transition temperature of the membrane, $t_c=41^{\circ}C$. CF fluorescent intensities are enhanced by the CF leakage from DPPC liposome at $45^{\circ}C$, while no changes are observed at $20^{\circ}C$. Under the same conditions, it is observed that the intensity enhanced by CF leakage from DPPC/DTAB liposome is larger than that of DPPC liposome alone, which suggests that DPPC/DTAB liposome has irregular arrangement. Under the presence of $Ca^{2+}$, Quin 2 fluorescent intensity in either DPPC liposome or DPPC/DTAB liposome is significantly increasing at $45^{\circ}C$, while almost none of the changes are observed at $20^{\circ}C$. The fluorescent intensity of DPPC liposome turns out to be larger than that of DPPC/DTAB liposome, which suggests that the DPPC/DTAB liposome is structurally more stable than the DPPC liposome. Additionally, when the analysis is done to observe changes in the shapes of membrane surfaces with ANS fluorescent, ANS fluorescent under DPPC or DPPC/DTAB liposome shows each of different appearances at $45^{\circ}C$ and $20^{\circ}C$ respectively. This result indicates that its respective membrane fluidity is changing above and below of the designated temperatures in phase transition. As to the magnitude of change of its membrane fluidity, DPPC liposome is much larger than DPPC/DTAB liposome. As far as the temperature in phase transition measured by DSC are concerned, it is $41^{\circ}C$ and $32^{\circ}C$ for DPPC and DPPC/DTAB liposome respectively, which suggests that DPPC/DTAB liposome has an irregular molecular arrangement in its structure. That is, it is summed up that DPPC/DTAB turns out to be structurally stable, even so, its structure is irregularly arranged.

• Journal of Korean Society of Disaster and Security
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• v.12 no.4
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• pp.43-52
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• 2019

To analyze the influence on the stability, resulting from application of upgrade pipe roof structure (UPRS) method to the structure existed under subway Station, physical properties of a ground, elasticity and elasto-plastic theories, including displacement analysis of finite elements, stress analysis of finite elements, displacement caused by steel pipe propulsion and internal excavation, and stress change in a steel pipe, were introduced. Then, the influence on structural stability when applying the UPRS method was compared and reviewed based on the construction management standard of the Ministry Land, Infrastructure and Transport and foreign sources, using numerical analysis with a model which assumes that each microelement divided into a structurally stable point consists of the connection of finite points. As a result of the finite element analysis, 7.21 mm maximum displacement, 1/3,950 angular displacement, 70.28 MPa bending compressive stress of steel pipe structure constructed with UPRS (non-excavation) method and 477.38 MPa maximum shear strength were within their allowable standards (25.00 mm, 1/500, 210.00 MPa and 120.00 MPa, respectively), and therefore, the results showed that the design and construction are stable.

• Korean Journal of Heritage: History & Science
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• v.46 no.3
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• pp.212-228
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• 2013

Stone cultural heritages are repaired by the use of metal stiffeners. The problem is that this type of repair has been based on the experience of workers without specific guidelines and has caused various problems. This is to suggest the structural reinforcement and behavioral characteristics of metal rods to minimize the secondary damage of materials and have the specimens tested and verified to establish the guidelines on how to insert metal stiffeners. When only epoxy resin is applied to the cut surface, only 70% of the properties of the parent material are regenerated and it is required to structurally reinforce the metal stiffener for the remaining 30%. The metal rod is under the structural behavior after the brittle failure of stone material and the structural behavior does not occur when the metal stiffener is below 0.251%. When it accounts for over 0.5%, it achieves structural reinforcement, but causes secondary damage of parent materials. The appropriate ratio of metal stiffener for the stone material with the strength of $1,500kgf/cm^2$, therefore, should be between 0.283% and 0.377% of the cross section of attached surface to achieve reversible fracture and ductility behavior. In addition, it is more effective to position the stiffeners at close intervals to achieve the peak stress of metal rod against bending load and inserting the stiffener into the upper secions is not structurally supportive, but would rather cause damage of the parent material. Thus, most stiffeners should be inserted into the lower part and some into the central part to work as a stable tensile material under the load stress. The dispersion effect of metal rods was influenced by the area of reinforcing rods and unrelated to their diameter. However, it ensures stability under the load stress to increase the number of stiffeners considering the cross section adhered when working on large-scale structures. The development length is engineered based upon the diameter of stiffener using the following formula: $l_d=\frac{a_tf_y}{u{\Sigma}_0}$. Also, helically-threaded reinforcing rods should be used to perform the behaviors as a structural material.

• Journal of the Korean Ceramic Society
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• v.41 no.12 s.271
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• pp.921-928
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• 2004

Calcium phosphate powders have been prepared by using $Ca(OH)_2\;and\;H_{3}PO_4$ solution under various conditions such as pH, calcination temperature, and reaction time. ${\beta}-TCP({\beta}-tricalcium phosphate)$and HAp(hydroxyapatite) were synthesized at pH=5.21 and pH > 7.62, respectively. From XRD results, $Ca(OH)_2\;and\;H_{3}PO_4$ solution reacted quickly to form HAp, which was structurally stable up to 16h. Calcination temperature having good crystallinity is revealed to be at $1200^{\circ}C$. SEM analysis showed that ${\beta}-TCP$ and HAp with needle type were synthesized at pH 5.21 and pH 7.62, respectively. However, at pH 9.16, tiny and homogeneous HAp having sphere was prepared and rearranged to show needle morphology. HAp synthesized at pH 9.16 was utilized as bonechina body and calcined. The sample was analyzed its crystallinity, water absorbtion, color, and shape to check physical properties.

• The Journal of the Korea Contents Association
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• v.17 no.4
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• pp.179-199
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• 2017

This research was conducted in a phenomenological method to explore what the meaning for marriage migrant women to have college experience is. In other words, It is to find out what the motivation for them to enroll the college is and what the experience of studying in college implies. Also, figuring out how the expectation for life after the college experience is built up structurally is the purpose of the research. To ensure the qualitative warranty of the research, the participants are selected by "Sampling and Data Collection Strategy" suggested by Miles & Huberman (1994); and those selected participants are six marriage migrant women in urban-rural area with 10~18 years of residence period. The information for this research had been collected by in-depth and additional interviews along with observation towards these six participants. The result is the following. First, the motivation for them to enroll the college is; to realize of one's dream, to have confident parental model, to pursuit stabilized domestic economy, to have a professional occupation, and to make it as a mean of searching one's career path. Second, the experience of studying in college implies; enhancing communication ability, establishing self-image in the family and its relationship, having confident in raising children, enhancing healthy living of family, enhancing human relations, and lifelong learning person through set of learning. Third, the structure of how it build the future life is; having independent life pursuing stable living economically, living as a confident Korean by pursuing the value of learning and having a life of human being.

• Korean Journal of Construction Engineering and Management
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• v.19 no.6
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• pp.115-123
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• 2018

The primary objective of this study is to develop an all-in-one based steel pipe pile cutting robot prototype that improves the conventional steel pipe pile head cutting work in safety, quality, and productivity. For this, the following research works are conducted sequentially; 1)literature review and expert survey, 2)selection of core technology using AHP analysis, 3)deduction of detail design, 4)verification of structural stability, 5)development of full-scale prototype. As a result leveling laser and laser detector(94.46), plasma cutter(96.72), rotary grapple(98.45) are selected as a core technologies. As an outcome, it is analyzed that gripper, cylinder pivot bracket and gripper base are structurally stable. Their maximum stresses are shown as 43.0%, 19.4%, 5.3% compared to their yield strength respectively. The development of full-scale prototype in this study will be utilized for the development of the all-in-one attachment based steel pipe pile cutting robot commercialization model.

• Korean Journal of Construction Engineering and Management
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• v.19 no.2
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• pp.117-125
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• 2018

The primary objectives of this study are to develop a detail design of automated PHC pile head cutting machine and structural stability analysis of detail design that improves the conventional head cutting work in safety, quality, and productivity. For this, the following research works are conducted sequentially; 1)literature review and field study, 2)expert survey and interview, 3)selection of core technology using AHP analysis, 4)deduction of detail design 5) verification of structural stability. As an outcome, it is analyzed that gripper and gripper bearing shaft are structurally stable. Their maximum stresses are shown as 15.93%, 10.58% compared to their yield strength respectively. The results of detail design and structural stability analysis in this study will be utilized for the actual development of the automated PHC pile cutting machine prototype.

• Journal of the Korea institute for structural maintenance and inspection
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• v.16 no.5
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• pp.137-147
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• 2012

In the steel-free bridge concrete deck, steel straps are generally used instead of conventional steel rebar while laterally restrained in the perpendicular direction to the traffic in order fir the arching effect of concrete deck. In this paper, the minimum amount of FRP bar is to be suggested based on the structural strength, crack propagation, stress level and others in order to control cracks. As a result of laboratory tests, the structural strength of deck with 0.15 percentage of steel strap showed improved structural strength including ductility. The long-term serviceability of steel strap deck with FRP bar proved to satisfy the requirements and to be structurally stable while showing the amount of crack and residual vertical displacement within the allowable limits after two million cyclic loadings. The structural failure of RC bridge deck is generally caused from the punching shear rather than moment. Therefore, the ultimate load at failure could be estimated using the shear strength formula in the two-way slab based on ACI and AASHTO criteria. However the design criteria tend to underestimate the shear strength since they don't consider the arching effects and nonlinear fracture in bridge deck with lateral confinement. In this paper, an equation to estimate the punching shear strength of steel strap deck is to be developed considering the actual failure geometries and effect of lateral confinement by strap while the results are verified in accordance with laboratory tests.

• Applied Microscopy
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• v.39 no.3
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• pp.253-260
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• 2009

GM-CSF is a multipotent growth factor, which also plays an important role during the process of wound healing. rrhGM-CSF was specifically produced from rice cell culture in our laboratory (Hanson Biotech Co., Ltd, Daejeon). The rrhGMCSF contains more oligosaccharide side chains than any other types of GM-CSF. This work was taken to evaluate the influence on wound healing of rrhGM-CSF in male golden hamsters. Full thickness skin defects of 9 mm in diameter were made in the back of hamsters, and 100 ${\mu}L$ ointment containing rrhGM-CSF 50 ${\mu}g/mL$ was applied. Control groups were given ointment without rrhGM-CSF. The wound sizes were relatively reduced and skin was well regenerated in the experimental group compared with the control group. Structurally, reepithelialization and architecture of the skin following injury were well accomplished in the experimental group. And also, positive reaction of PCNA of the skin following injury was more prominent in rrhGM-CSF containing ointment treatment group. Since this type of GM-CSF has highly glycosylated side chains, the effectiveness might be retain longer and stable, regarding acceleration of wound healing in the animal model. The present study has important implications for further development of the therapeutic manipulation of wound healing using rrhGM-CSF.

• Journal of Microbiology and Biotechnology
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• v.22 no.12
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• pp.1636-1643
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• 2012

Enzymatic pre-bleaching by modification of pulp fibers with xylanases is an attractive approach to reduce the consumption of toxic bleaching chemicals in the paper industry. In this study, an alkaliphilic endoxylanase gene was isolated from metagenomic DNA of a structurally stable thermophilic lignocellulose-degrading microbial consortium using amplification with conserved glycosyl hydrolase family 10 primers and subsequent genome walking. The full-length xylanase showed 78% sequence identity to an endo-${\beta}$-1,4-xylanase of Clostridium phytofermentans and was expressed in a mature form with an N-terminal His6 tag fusion in Escherichia coli. The recombinant xylanase Xyn3F was thermotolerant and alkaliphilic, working optimally at $65-70^{\circ}C$ with an optimal pH at 9-10 and retaining >80% activity at pH 9, $60^{\circ}C$ for 1 h. Xyn3F showed a $V_{max}$ of 2,327 IU/mg and $K_m$ of 3.5 mg/ml on birchwood xylan. Pre-bleaching of industrial eucalyptus pulp with no prior pH adjustment (pH 9) using Xyn3F at 50 IU/g dried pulp led to 4.5-5.1% increase in final pulp brightness and 90.4-102.4% increase in whiteness after a single-step hypochlorite bleaching over the untreated pulp, which allowed at least 20% decrease in hypochlorite consumption to achieve the same final bleaching indices. The alkaliphilic xylanase is promising for application in an environmentally friendly bleaching step of kraft and soda pulps with no requirement for pH adjustment, leading to improved economic feasibility of the process.