• Journal of Microbiology and Biotechnology
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• v.20 no.2
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• pp.254-264
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• 2010

To obtain an efficient natural lignocellulolytic complex enzyme, we screened an efficient lignocellulose-degrading composite microbial system (XDC-2) from composted agricultural and animal wastes amended soil following a long-term directed acclimation. Not only could the XDC-2 degrade natural lignocelluloses, but it could also secrete extracellular xylanase efficiently in liquid culture under static conditions at room temperature. The XDC-2 degraded rice straw by 60.3% after fermentation for 15 days. Hemicelluloses were decomposed effectively, whereas the extracellular xylanase activity was dominant with an activity of 8.357 U/ml on day 6 of the fermentation period. The extracellular crude enzyme noticeably hydrolyzed natural lignocelluloses. The optimum temperature and pH for the xylanase activity were $40^{\circ}C$ and 6.0. However, the xylanase was activated in a wide pH range of 3.0-10.0, and retained more than 80% of its activity at $25-35^{\circ}C$ and pH 5.0-8.0 after three days of incubation in liquid culture under static conditions. PCR-DGGE analysis of successive subcultures indicated that the XDC-2 was structurally stable over long-term restricted and directed cultivation. Analysis of the 168 rRNA gene clone library showed that the XDC-2 was mainly composed of mesophilic bacteria related to the genera Clostridium, Bacteroides, Alcaligenes, Pseudomonas, etc. Our results offer a new approach to exploring efficient lignocellulolytic enzymes by constructing a high-performance composite microbial system with synergistic complex enzymes.

• Journal of the Korean Geotechnical Society
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• v.32 no.12
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• pp.45-57
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• 2016

The Inclined Earth Retaining Structure (IER) is the structure using an integrated system of both front supports and inclined back supports to increase the stability for excavation. The IER is a structurally stable temporary excavation method using the back supports restraining the lateral displacement of the front supports as stabilizing piles. The back supports connected to the front supports significantly reduce the earth pressure acting on both the front wall and the front supports by distributing it to the back supports in order to increase the structural stability. In this study, mechanical behaviors of IER according to the head connection type using fixed- or hinge-connection were found by performing numerical analysis and laboratory model tests in the sandy ground. The maximum lateral displacement of fixed-connection was 88% of that of hinge-connection in the numerical analysis. The lateral displacement of fixed-connection was 7% of that of hinge-connection in the laboratory model test results. Furthermore, the earth pressure of the fixed-connection was 67% of that of the hinge-connection in the shear-strain analysis results of the model ground.

• Journal of the Korea Convergence Society
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• v.10 no.12
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• pp.281-286
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• 2019

To repair the underside of the car, a repairman has to enter under the car body. But this work can make it difficult for him to fix it and the injuries can occur. To solve these difficult problems, the developed equipment is the automotive lift. In this study, three kinds of lift models 1, 2 and 3 were designed and the material properties of the structural steel were applied. As the same load were applied under the same conditions on all models, the structural analyses were conducted. Models 2 and 3 were shown to have the structural deformation less than model 1. Also, models 2 and 3 were shown to be more stable than model 1 structurally. By utilizing the design data on a convergence research through durability analysis according to the configuration of automotive lift obtained on the basis of this result, the esthetic feeling can be shown by being converged onto the automotive repair equipment parts at actual life.

• BMB Reports
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• v.34 no.4
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• pp.293-298
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• 2001

Tryptic activation of the 130-kDa Bacillus thuringiensis Cry4B $\delta$-endotoxin produced protease-resistant products of ca. 47 kDa and ca. 21 kDa. The 21-kDa fragment was identified as the N-terminal five-helix bundle (${\alpha}1-{\alpha}5$,) which is a potential candidate for membrane insertion and pore formation. In this study, we constructed the recombinant clone over-expressing this putative pore-forming (PPF) fragment as inclusion bodies in Escherichia coli. The partially purified inclusions were composed of a 23-kDa protein, which cross-reacted with Cry4B antibodies, and whose N-terminus was identical to that of the 130-kDa protein. Dissimilar to protoxin inclusions, the PPF inclusions were only soluble when the carbonate buffer, pH 9.0, was supplemented with 6 M urea. After renaturation via a stepwise dialysis, the refolded PPF protein appeared to exist as an oligomer and was structurally stable upon trypsin treatment. Unlike the 130kDa protoxin, the refolded protein was able to release entrapped glucose from liposomes, and showed comparable activity to the full-length activated toxin, although it lacks larvicidal activity These results, therefore, support the notion that the PPF fragment that consists of ${\alpha}1-{\alpha}5$ of the activated Cry4B toxin is involved in membrane pore-formation.

• Journal of the Mineralogical Society of Korea
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• v.15 no.2
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• pp.140-144
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• 2002

The sample environment at high temperature for the four circle diffractometer at HANARO in Korea Atomic Energy Research Institute is developed. The performance test was carried out for a structurally known sample through the high temperature experiment with this equipment. In this study we found out that the developed sample environment is stable for a long time experiment at over 900 K. By the neutron diffraction from a single crystal of $LiTaO_3$(phase transition temperature about 900 K) at 298 and 913 K, the lithium atomic positions at both temperatures and disordered state of lithium atom at high temperature were confirmed. These are hardly possible to determine by the conventional X-ray diffraction method.

• Microbiology and Biotechnology Letters
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• v.41 no.1
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• pp.60-69
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• 2013

Purified melanin pigment from Klebsiella sp. GSK was characterized by thermogravimetric, differential thermal, X-ray diffraction and elemental analysis. This melanin pigment is structurally amorphous in nature. It is thermally stable up to $300^{\circ}C$ and emits a strong exothermic peak at $700^{\circ}C$. Its carbon, hydrogen and nitrogen composition is 47.9%, 6.9% and 12.0%, respectively. It was used to scavenge metal ions and free radicals. After immobilizing the pigment and using it to adsorb copper and lead ions, the metal ion adsorption capacity was evaluated by atomic absorption spectroscopy (AAS) and the identity of melanin functional groups involved in the binding of metal ions was determined by Fourier transform infrared (FT-IR) spectroscopy. Batch adsorption studies showed that 169 mg/g of copper and 280 mg/g of lead were adsorbed onto melanin-alginate beads. The metal ion adsorption capacity of the melanin-alginate beads was relatively significant compared to alginate beads. The metal ion desorption capacity of HCl was greater (81.5% and 99% for copper and lead, respectively) than that of EDTA (80% and 71% for copper and lead, respectively). The ability of the melanin pigment to scavenge free radicals was evaluated by inhibition of the oxidation of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and was shown to be about 74% and 98%, respectively, compared with standard antioxidants.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.40 no.7
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• pp.679-683
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• 2016

Boilers of large-sized coal-fired power plants are being operated under very poor conditions such as continuous operation or repeating of start-up and shutdown for a stable supply of electricity. Thus periodic inspection and maintenance are required to ensure reliability of operation. The loads of existing scaffolding systems for the maintenance of boilers are concentrated in the lower parts structurally, which may cause a serial collapse of the overall scaffolding system when there are problems in some members. Therefore, in this study, a safe furnace scaffolding system is developed by dispersing the loads in the upper part, as well as minimizing the hazards of serial collapsing. In addition, for cases where the direct installation of furnace scaffolding is challenging owing to the structure of the boiler tube, a lifting system for the installation of furnace scaffolding is developed so that furnace scaffolding can be supported to secure the integrity of the power generating facility.

• Journal of the Korean Society of Hazard Mitigation
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• v.6 no.4 s.23
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• pp.1-6
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• 2006

In terms of the speed-up of the conventional line, the research for a improved turnout was carried out continually around world. A turnout is known that it is the most unstable equipment structurally in the rail system. So, the turnout is needed more stable structure. An accurate assembly of turnout affects traveling qualify of turnout area and running safety. Therefore, a turnout which is pre-assembled at a factory by using the split PC sleeper is transported to construction site recently in advanced countries. In this paper, the performance measurements which estimate the safety of the split PC sleepers which are adopted for the first time to improve performance of turnout are implemented indoors. The split PC sleeper is one of components of the improved turnout. The test is considered of the worst ballast condition under the maintenance work as like STT(Swithc Tie Tamper) or MTT(Multiple Tie Tamper). These experiments results indicate that structural safety for the expansion joint of the split PC sleepers.

• Proceedings of KOSOMES biannual meeting
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• 2005.05a
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• pp.155-159
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• 2005

Analysis target ship is not introduced yet in domestic as steel yacht that is getting into the spotlight by leisure life in Australia or Japan. Sailing yacht or Yacht for leisure time made of FRP into controlling power fare mainly and the design and made of latest fishing boat and something of domestic is consisting mainly. To need investigated for concept is various kinds overall strength as that use mainly steel wire material structurally of steel yacht by small crafts about Longi strength, Transe strength portion even of design safety factor at subject to do Rule's allowable stress enough stable structure accomplish. But it is assessment of part intensity that become refer to most in small size ship.

• Geotechnical Engineering
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• v.2 no.3
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• pp.51-66
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• 1986

The design of fabric reinforced retaining wall structure was discussed in this article. It was confirmed that the reinforced retaining earth wall which was designed by new theoretical formulae developed this time was stable structurally and economically. The plastic fabric filter which was placed in layers behind the facing element reduced the lateral earth pressure on the wall elements in comparison with a conventional retaining earth walls. The reinforcing characteristics of earth wall was governed by the spacing of fabric layers, effective length of fabrics, particle distribution and compaction, and thus it is essential that, in the construction field, the reinforcing strips should be selected in order to develop the maximum friction forces bet.eon soil and fabric filters. The maximum tensile stress developed from the reinforcing strips was appeared at a little far distance from the back of skin element and it was not well agreed with the Rankine's theory but distributed well as a symmetrical shape against the point of the maximum tensile stress. The total length of the different layers should be sufficient so that the tension in the fabric strip could be transferred to the backfill material. Also the total stability of reinforced earth wall should be checked with respect to a failure surface which extended blond the different lathers.