• Environmental Analysis Health and Toxicology
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• 제19권3호
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• pp.251-259
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• 2004

Xenoestrogens are chemicals with diverse structure that mimic estrogen. Bisphenol A, a monomer of polycarbonate and epoxy resins, has been detected in canned food and human saliva. Bisphenol A stimulate cell proliferation and induce expression of estrogen -response genes in vitro. The purpose of the this study was to evaluate cell proliferation of bisphenol A in the presence of a rat liver 59 mix contaning cytochrome P450 enzymes and Cu (II). The fragmentation of intact DNA, a parameter of apoptotic cell death, was evaluated quantitatively by diphenylamine reaction method. Bisphenol A induced apoptotic cell death in a dose-dependent manner The effect of radical scavenger on the apoptotic cell death induced bisphenol A was investigated. The DNA fragmentation induced by bisphenol A was significantly inhibited by addition of radical scavenger to the culture medium. This indicated that elevated oxidative stress caused by imbalance between the production and removal of free radicals occurred in cells. Taken together, these results suggest that free radical reacts with Cu (II) leading oxidative stress.

• 한국공작기계학회:학술대회논문집
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• 한국공작기계학회 1999년도 춘계학술대회 논문집
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• pp.163-169
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• 1999

Residual stresses causing stress corrosion cracking (SCC) of thin-walled steam generator U tubes were investigated. The residual stresses were measured by hole drilling methods, and the applied stresses resulting from the internal pressure and the temperature gradient in the steam generator were estimated theoretically. In U-bent regions, the residual stresses at extrados were induced with compressive stress(-), and its maximum value reached -319MPa in axial direction at $\phi$= $0^{\circ}$ in position. Maximum tensile residual stress of 170MPa was found to be at the flank side at position of $\phi$= $90^{\circ}$, i.e., at apex region. Hoop stress due to the pressure and temperature differences between primary and secondary side were analyzed to be 76 MPa and 45 MPa, respectively.

• 동의신경정신과학회지
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• 제18권1호
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• pp.95-110
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• 2007

Objective : The purpose of this study was to investigate molecular basis of neuroprotective effect in total ginsenosides. After H202 induced neurotoxicity, gene expression profiling of SH-SY5Y neuroblastoma cells treated by total ginsenosides is analyzed. Method : After SH-SY5Y cells were cultured, they were damaged by H202 induced oxidative stress. After twenty four hours, experimental group is treated by total ginsenosides and control group is treated by 0.9% saline. A high density cDNA microarray chip is used to analyze the gene expression profiling of SH-SY5Y cells. The Significance Analysis of Microarray method is used for identifying genes on a microarray. Results : 1. According to the results of microarray experiment, 17 genes were up-regulated, 38 genes were down-regulated. 2. Expression of OPHNl, KTANl, ATM, PRKCE, MAPKs genes associated with cell proliferation, neural growth, and the prevention of apoptosis were increased. 3. Change of EPX gene was the greatest among all genes. EPX gene associated with oxidative stress, and tumor suppressor gene ADAM11 were decreased. Conclusion : According to this study, molecular basis of neuroprotective effect of total ginsenosides is as followings: the increase of gene expression associated with cell proliferation, neuron growth, the prevention of apoptotsis and decrease of gene expression associated with oxidative stress and tumor suppressor.

• 대한한방내과학회지
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• 제43권3호
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• pp.344-362
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• 2022

Objective: In this study, the antioxidant activity of Ojayeonjong-hwan extracts was compared, and the following results were obtained. Methods: For hydrothermal and ethanol extracts, DPPH free radical and ABTS cationic radical erasing activity and reducing power using the FRAP method were compared, and the association between the antioxidant power of each extract and total phenol content was investigated. Significant results were obtained through in vitro apoptosis analysis through FFITC staining, mitochondrial membrane potential analysis, and ROS level measurement using C2C12 myoblastoma. Results: 1. In a comparison of DPPH free radical and ABTS cationic radical scavenging activity, water, and 70% ethanol extracts of Ojayeonjong-hwan (WEO and EEO) showed superior radical scavenging ability. 2. In the results of reducing power using the FRAP method, WEO and EEO showed antioxidant activity, which was shown to be dependent on the total phenol content contained in the extracts. 3. In comparison to the protective effect against H₂O₂-induced oxidative stress in C2C12 myoblasts, water extracts had no significant effect, but 70% ethanol extracts inhibited H₂O₂-mediated cytotoxicity in a concentration-dependent manner. 4. The cytotoxic protective effect of EEO against oxidative stress in C2C12 myoblasts was correlated with its inhibitory effects on H₂O₂-induced apoptosis and cell-cycle arrest. 5. In H₂O₂-treated C2C12 myoblasts, the apoptosis inhibitory effects of EEO were associated with the suppression of mitochondrial dysfunction and DNA damage. 6. The protective effects of EEO against H₂O₂-induced oxidative stress in C2C12 myoblasts were directly related to the inhibition of ROS generation. Conclusions: Ojayeonjong-hwan extracts all have protective potential against oxidative stress.

• 한국구조물진단유지관리공학회 논문집
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• 제23권6호
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• pp.45-52
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• 2019

이산화탄소 농도가 높은 도심지의 경우 탄산화로 인한 철근부식이 발생하기 쉬우며 이는 콘크리트 구조물의 내구수명을 감소시킨다. 콘크리트 구조물의 경우 다양한 구속조건을 가지며 항상 외부의 재하하중을 받고 있다. 도입된 응력수준은 이산화탄소와 같은 유해인자의 확산을 변화시키며 탄산화 깊이의 변동성을 야기한다. 본 연구에서는 응력재하수준에 따른 탄산화 변동성을 정량화하였으며, 이를 이용하여 탄산화 예측식을 도출하였다. 내구성 설계인자인 피복두께, 이산화탄소 확산계수, 탄산화 반응 수화물, 그리고 외부 이산화탄소 농도를 확률변수로 정의하였으며, MCS을 통하여 영향인자의 변동성에 따른 내구수명을 도출하였다. 또한 응력수준에 따라 변화하는 내구수명을 도출하였으며, 이를 결정론적인 방법의 결과와 비교하였다. 피복두께 및 내부 수화물 생성이 내구수명 변동성에 가장 큰 영향을 미쳤으며, 응력수준을 고려한 내구수명평가는 유지관리 우선순위 설정에 합리적으로 적용할 수 있다.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 1994년도 봄 학술발표회 논문집
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• pp.13-18
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• 1994

Most widened bridges have been constructed by the joining-construction method that makes new and existing bridge structurally single structure. However, joing-construction method has several problems in design and construction viewpoint. Therefore, this study is conducted in order to investigate structural behavior of widened RC slab and traffic-induced vibration of existing bridge during placing and curing of new concrete by the prototype flexural fatigue test. From the results of this study, it is shown that stress-concentration and slip occur between concrete and reinforcing rod at joint section but the reduction of load carrying capacity and of fatigue strength is negligible according to the traffic-induced vibration as well as the difference of construction method. A reasonable construction method for the bridge widening which takes into account the effects of the traffic-induced vibration and S-N curve for the widened bridge are also proposed.

• Geomechanics and Engineering
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• 제20권2호
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• pp.165-174
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• 2020

The enzyme-induced carbonate precipitation (EICP) method has been investigated to improve the hydro-mechanical properties of natural soil deposits. This study was conducted to explore the stiffness evolution during various stress scenarios. First, the optimal concentration of urea, CaCl₂, and urease for the maximum efficiency of calcite precipitation was identified. The results show that the optimal recipe is 0.5 g/L and 0.9 g/L of urease for 0.5 M CaCl₂ and 1 M CaCl₂ solutions with a urea-CaCl₂ molar ratio of 1.5. The shear stiffness of EICP-treated sands remains constant up to debonding stresses, and further loading induces the reduction of S-wave velocity. It was also found that the debonding stress at which stiffness loss occurs depends on the void ratio, not on cementation solution. Repeated loading-unloading deteriorates the bonding quality, thereby reducing the debonding stress. Scanning electron microscopy and X-ray images reveal that higher concentrations of CaCl₂ solution facilitate heterogeneous nucleation to form larger CaCO₃ nodules and 11-12 % of CaCO₃ forms at the interparticle contact as the main contributor to the evolution of shear stiffness.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1996년도 춘계학술대회
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• pp.97-99
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• 1996

Intracellular gradients of the free calcium concentration are thought to be critical for the localization of functional responses within a cell. The mechanism of mechanotransduction may be associated with the localized accumulation of calcium stores for shear stress-exposed endothelial cells. The distribution of the calcium stores and the formation of the stress fibers were investigated by the indirect double immunofluorescent staining method with the calreticulin antibody and rhodamine phalloidin under flow condition. The shear stress of steady flow reorganized the cytoskeleton structure including the bundling and translocation to focal contacts. The calcium stores translocated from the cytoplasm to the focal contacting area. Consequently. accumulation of the calcium stores may participate in the shear stress-induced cytoskeleton organization of endothelial cells.

• Geomechanics and Engineering
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• 제8권6호
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• pp.783-799
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• 2015

In this study, effect of horizontal in situ stress on failure mechanism around underground openings excavated in isotropic, elastic rock zones is investigated. For estimating the plastic zone occurrence, an induced stress influence area approach (Bray Equations) was modified to define critical stress ratio according to the Mohr-Coulomb failure criterion. Results obtained from modified calculations were compared with results of some other analytical solutions for plastic zone thickness estimation and the numerical modelling (finite difference method software, FLAC2D) study. Plastic zone and its geometry around tunnels were analyzed for different in situ stress conditions. The modified equations gave similar results with those obtained from the other approaches. However, safer results were calculated using the modified equations for high in situ stress conditions and excessive ratio of horizontal to vertical in situ stresses. As the outcome of this study, the modified equations are suggested to use for estimating the plastic zone occurrence and its thickness around the tunnels with circular cross-section.

• 한국지역사회생활과학회지
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• 제12권2호
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• pp.33-45
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• 2001

This study was performed to investigate the degree of different stresses in university students of outer region of Seoul and to search the influence of food habit and nutrient intakes in stress. The data was collected by questionnaire method for self-diagnosed different stresses, dietary habit, and physical status and 24 hours recall for nutrient intakes. The subjects were 282 students attended at the lecture Food and Health. Diet balance was no difference with gender but food intakes were more diverse in female. Nutrient intake of male students was lower in vitamin A and riboflavin, but that of female was low in vitamin A and iron. The university students outer region of Seoul had more stress situation in frustration, deprivation and self-efface than in the stress of the noise and Type-A behavioral stress. Total self-diagnosed dietary habit score was better in the group of no susceptive overload stress than that of the high susceptive group. Same trend of dietary behavior score was in the group of no noise and anxiety-response stress than that in the susceptive groups. The quantity of nutrient intakes was correlated with different kind of stress. Niacin intake was positively related with the stress from life-events, and food induced stress. Thiamin intake was positively related with food induced stress. But the intake of iron, calcium, niacin and riboflavin were negatively related with the stress of noise. Therefore, we could concluded that different kind of stresses were influenced in dietary behavior and vitamin-mineral nutrient intakes. More research would be needed at the aspects of health promotion in the stressful situation of modern society.