Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
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Gene expression profiling of SH -SY5Y cells in neuroprotective effect of total ginsenosides on H202 induced neurotoxicity

인간 신경모세포종 SH-SY5Y에서 인삼(人蔘) total ginsenosides의 신경보호 기능에 관련된 유전자 발현 양상에 대한 연구

  • Lee, Seung-Gi (Dept. of Neuropsychiatry, College of Oriental Medicine, Sang Ji university) ;
  • Chai, Young-Gyu (Division of Molecular & Life science, Han Yang university) ;
  • Jung, Kyoung-Hwa (Division of Molecular & Life science, Han Yang university) ;
  • Kim, Ji-Hyouck (Rapha Kyunghee Clinic of Oriental Medicine) ;
  • Hu, Yong-Suk (Dept. of Neuropsychiatry, College of Oriental Medicine, Sang Ji university)
  • 이승기 (상지대학교 한의과대학 신경정신과) ;
  • 채영규 (한앙대학교 분자생명과학부) ;
  • 정경화 (한앙대학교 분자생명과학부) ;
  • 김지혁 (라파경희한의원) ;
  • 허용석 (상지대학교 한의과대학 신경정신과)
  • Published : 2007.03.30
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Abstract

Objective : The purpose of this study was to investigate molecular basis of neuroprotective effect in total ginsenosides. After H202 induced neurotoxicity, gene expression profiling of SH-SY5Y neuroblastoma cells treated by total ginsenosides is analyzed. Method : After SH-SY5Y cells were cultured, they were damaged by H202 induced oxidative stress. After twenty four hours, experimental group is treated by total ginsenosides and control group is treated by 0.9% saline. A high density cDNA microarray chip is used to analyze the gene expression profiling of SH-SY5Y cells. The Significance Analysis of Microarray method is used for identifying genes on a microarray. Results : 1. According to the results of microarray experiment, 17 genes were up-regulated, 38 genes were down-regulated. 2. Expression of OPHNl, KTANl, ATM, PRKCE, MAPKs genes associated with cell proliferation, neural growth, and the prevention of apoptosis were increased. 3. Change of EPX gene was the greatest among all genes. EPX gene associated with oxidative stress, and tumor suppressor gene ADAM11 were decreased. Conclusion : According to this study, molecular basis of neuroprotective effect of total ginsenosides is as followings: the increase of gene expression associated with cell proliferation, neuron growth, the prevention of apoptotsis and decrease of gene expression associated with oxidative stress and tumor suppressor.

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