• 한국가금학회:학술대회논문집
• /
• 한국가금학회 2005년도 제22차 정기총회 및 학술발표회
• /
• pp.8-16
• /
• 2005

비만유전자의 산물인 렙틴은 주로 지방세포에서 생성 분비된다. 렙틴은 뇌 시상하부에 작용하여 식이섭취량을 줄이고 에너지발산을 증가시켜 궁극적으로 체중을 감소시킨다. 따라서 렙틴 또는 렙틴 수용체의 이상은 극도의 대사증후군을 유발한다. 현재까지 렙틴에 관한 연구의 대부분은 사람을 포함한 포유류에서 수행되었다. 렙틴의 생리적 작용은 만복인자뿐만이 아니다. 본고에서는 현재까지 발표된 논문을 중심으로 닭에서 식이섭취, 면역, 지방대사, 번식과 발달, 그리고 스트레스에 관한 렙틴의작용을 검토했다.

• 자연과학논문집
• /
• 제15권1호
• /
• pp.57-67
• /
• 2005

산화적 스트레스에 대한 Bacillus subtilis의 thiol peroxidase 유전자의 생리적인 기능을 연구하기 위해 thiol peroxidase 유전자의 기능이 손상된 녹아웃 돌연변이주를 상동성 재조합에 의해 제조하였다. 호기적 조건에서 배양할 때 야생형과 녹아웃 돌연변이주 사이에는 성장속도에서 차이를 관찰할 수 없었다. 그러나 paraquat 처리할 때와는 달리 $H_2O_2$와 cumene hydroperoxide (CHP)에 의한 산화적 스트레스에 대해 역할을 하고 있음을 시사하는 결과이다.

• 한국산학기술학회:학술대회논문집
• /
• 한국산학기술학회 2010년도 춘계학술발표논문집 2부
• /
• pp.1221-1223
• /
• 2010

본 논문에서는 한국인 아동의 우식치아로부터 S. mutans를 분리하고, acid stress하에서 분리한 S. mutans의 유전자의 발현의 변화를 분석하고자 하였다. 치아우식증의 주요한 요소로 작용하는 치태형성에 기여하는 glucan 및 fructan 합성에 관여하는 세포내 효소인 glucosyltransferase, glucosyltransferase, glucosyltransferase 및 fructosyltransferase의 발현량의 변화를 확인한 결과, lactic acid를 처리하지 않은 control의 경우보다 16배에서 3배까지 감소한 것을 확인할 수 있었다. Amino acid ABC transporter, adenylate kinase, fructokinase, 40k cell wall protein precursor에서는 모두 유전자의 발현량이 현저히 증가한 것을 볼 수 있었다. 이들 유전자는 acid stress에 관여하는 특이적 유전자로 추정된다.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제11권6호
• /
• pp.516-521
• /
• 2006

The use of gltA gene, as a new biomarker for environmental stress biomonitoring, was investigated because of its key position as the first enzyme of the tricarboxylic acid (TCA) cycle. A recombinant bioluminescent Escherichia coli strain, EBJM2, was constructed using a plasmid carrying the citrate synthase (gltA) promoter transcribing the Photorhabdus luminescens IuxCDABE genes (gltA::luxCDABE). The responses from this strain were studied with five different classes of toxicants: DNA damage chemicals, phenolics, oxidative-stress chemicals, PAHs, and organic solvents. EBJM2 responded strongly to DNA damage chemicals, such as mitomycin C (MMC) and methyl-nitro-nitrosoguanidine (MNNG) and nalidixic acid with the strongest responses. In contrast, tests with several compounds from the other four classes of toxicants gave no significant response. Therefore, EBJM2 was found to be sensitive to DNA damage chemicals.

• 한국초지학회:학술대회논문집
• /
• 한국초지조사료학회 2005년도 학술심포지엄, 제43회 학술발표회
• /
• pp.150-151
• /
• 2005

• Biomolecules & Therapeutics
• /
• 제32권4호
• /
• pp.451-459
• /
• 2024

Apoptosis signal-regulating kinase 1 (ASK1) is an upstream signaling molecule in oxidative stress-induced responses. Because oxidative stress is involved in asthma pathogenesis, ASK1 gene deficiency was investigated in animal models of allergic asthma. However, there is no study to investigate whether ASK1 inhibitors could be applied for asthma to date. Selonsertib, a potent and selective ASK1 inhibitor, was applied to BALB/c mice of an ovalbumin (OVA)-induced allergic asthma model. Selonsertib suppressed antigen-induced degranulation of RBL-2H3 mast cells in a concentration-dependent manner. The administration of selonsertib both before OVA sensitization and OVA challenge significantly reduced airway hyperresponsiveness, and suppressed eosinophil numbers and inflammatory cytokine levels in the bronchoalveolar lavage fluid. Histopathologic examination elucidated less inflammatory responses and reduced mucin-producing cells around the peribronchial regions of the lungs. Selonsertib also suppressed the IgE levels in serum and the protein levels of IL-13 in the bronchoalveolar lavage fluid. These results suggest that selonsertib may ameliorate allergic asthma by suppressing immune responses and be applicable to allergic asthma.

• 한국초지조사료학회지
• /
• 제25권4호
• /
• pp.281-286
• /
• 2005

환경스트레스에 내성을 갖는 버즈풋 트레포일(Lotus crniculatus L.) 형질전환체를 개발하기 위하여, AtNDPK 유전자가 SWPA2 프로모터에 의해 조절되도록 재조합한 발현벡터 pCAMBIA 2300 /SWPA2::AtNDPK2를 Agrobacterium 형질전환 방법으로 버즈풋 트레포일에 도입하였다. Apgrobacterium과 버즈풋 트레포일 캘러스의 공동배양한 캘러스를 $100{\mu}g/m1$의 kanamycin 및 $500{\mu}g/ml$의 cefotaxim을 첨가한 SH-3-kc 배지에서 배양하며 형질전환된 캘러스를 선발한 다음, BOi2Y 배지에서 2개월 이상 배양하며 식물체로 재분화시켰다. 재분화된 버즈풋 트레포일의 genomic DNA를 분리, PCR 및 Southern blot 분석을 실시한 결과, AtNDPK유전자를 도입한 형질전환체의 경우 agarose gel 전기영동 및 X-ray 필름상에서 DNA band 및 hybridization signal을 확인할 수 있었으나, 형질전환 되지 않은 대조구의 버즈풋 트레포일에서는 DNA band 및 hybridization signal이 관찰되지 않았다.

• Journal of Animal Science and Technology
• /
• 제65권6호
• /
• pp.1308-1322
• /
• 2023

Minerals is required small amounts among various nutrients, but it has a significant impact on sow longevity and reproduction performance. This study was carried out to see the beneficial effects of marine-derived Ca-Mg complex on the reproductive performance of sows during four-parity periods. Seventy-two gilts ([Yorkshire × Landrace] × Duroc), with an average body weight of 181 kg, were randomly allocated to three groups; CON (basal diet), 0.3LC (CON - MgO - 0.3% limestone + 0.4% Ca-Mg complex), and 0.7LC (CON - MgO - 0.7% limestone + 0.4% Ca-Mg complex). During parity 3 and 4, the expression level of SCD gene was lower in the umbilical cord of piglets born to 0.3LC and 0.7LC sows compared with the CON sows. During parity 2, 3 and 4, SLC2A2 and FABP4 gene expressions were higher in the umbilical cord of piglets born to 0.7LC sows and the placenta of sows from 0.3LC groups, respectively. Ca-Mg complex increased (p < 0.05) Ca and Mg concentrations in sows and their piglets' serum as well as in colostrum regardless of parities. The serum vitamin D concentration was higher (p < 0.05) in their first parity, whereas serum prolactin and estrogen concentrations were higher (p < 0.05) during the fourth and third parity, respectively. The growth hormone concentrations were higher (p < 0.05) in the piglets born to sows during the first and second parity. The fat and immunoglobulin A (IgA) concentrations in colostrum were higher (p < 0.05) during the third and fourth parity, respectively. A reduction (p < 0.05) in salivary cortisol, epinephrine, and norepinephrine concentrations was observed in 0.3LC and 0.7LC sow groups compared with CON after farrowing regardless of parity, however before farrowing, a reduction in norepinephrine was observed. Before farrowing, the epinephrine and norepinephrine concentrations were higher (p < 0.05) during the first and second parity. After farrowing, the concentration of these hormones was higher during the second parity. Taken together, sows' parity and dietary Ca-Mg complex supplementation influenced serum metabolites, colostrum nutrients, stress hormones as well as the gene expressions related to lipid and glucose metabolism.

• 한국응용곤충학회:학술대회논문집
• /
• 한국응용곤충학회 2004년도 춘계 학술발표회
• /
• pp.53-53
• /
• 2004

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2006년도 Grop Science and Biotechnology in the 21st Century
• /
• pp.32.1-32.1
• /
• 2006