• 한국식품과학회지
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• 제43권6호
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• pp.783-786
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• 2011

버섯류는 기호식품으로서의 성격과 기능성식품으로서의 성격을 동시에 갖는 매우 독특한 식품소재로서 본 연구에서는 국내에서 자생하는 8종류의 버섯에서 항충치 활성성분을 조사해 보았으며, 더불어 높은 항충치활성을 가진 추출물을 얻고자 다양한 용매를 이용, 최적의 용매조성을 탐색해 보았다. Chloroform, methanol, acetone, ethyl acetate 및 증류수를 용매로 사용하였으며, 이들 추출물의 S. mutans에 대한 항균 효과와 glucosyltransferase (GTase) 활성 억제효과를 살펴보았다. 표고버섯, 영지버섯 및 만가닥버섯 추출물이 상대적으로 높은 항충치활성을 나타내었으며, 추출용매에 의한 항균효과를 조사해본 결과, chloroform, methanol, ethyl acetate로 추출한 버섯 추출물에서 상대적으로 높은 항균효과를 보여주었다. 8종의 버섯 중 항균효과가 뛰어나고 GTase 활성 억제효과가 높은 만가닥(Lyopyllum ulmarium) 버섯으로부터 chloroform, methanol 및 ethyl acetate 추출물을 얻어 항충치활성을 조사하였다. S. mutans에 대한 만가닥 버섯의 최대 항균효과를 얻기 위해 심플렉스 중심 합성 계획법(simplex centroid design)을 이용하여 최적의 추출용매조성을 살펴본 결과, chloroform, methanol 및 ethyl acetate 3가지 용매를 모두 동일한 비율(1:1:1)로 혼합하여 추출한 추출물에서 가장 높은 항균효과가 나타났다. 만가닥버섯추출물은 항충치 소재로서 중요한 역할을 할 수 있을 것으로 기대되며, 버섯추출물의 항충치 효과는 추출용매에 의해 영향을 받으므로 버섯에서 항충치활성물질을 얻고자 할 때에는 추출용매선택이 매우 중요할 것으로 사료된다.

• 농업과학연구
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• 제24권1호
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• pp.50-54
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• 1997

맥반석 (Holon Killer Sand, Letsoken, Nikko Co.)의 성분분석 및 그 추출물의 항균활성을 실험한 결과는 다음과 같다. 실험에 사용한 맥반석의 무기질 조성은 아연(Zn)이 가장 많이 함유되어 있었으나 인체에 유해성 무기성분은 함유되어 있지 않았다. 항균활성 실험에서 24시간 배양한 세균의 활성은 Bacillus subtilis가 가장 크게 나타났으며, 다음은 Stapylococcus aureus순이었고, Stapylococcus mutans는 항균력이 거의 없었다. 또한 각 세균에 대한 추출물 농도별 항균활성은 추출물의 농도에 따라 강한 항균활성을 나타내었다. 한편 수삼 부패균을 분류하여 이에 대한 맥반석 추출물의 항균활성을 측정한 결과 수삼 부패균에 대하여 맥반석 추출물은 항균활성이 인정되었다.

• 한국식품위생안전성학회지
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• 제15권4호
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• pp.277-281
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• 2000

한천은 비교적 풍부한 수산자원의 하나이나 그 가공 수준은 매우 미약하여 상당수가 폐기되고 있다. 한천의 이용률과 부가가치를 높이기 위하여 한천올리고당의 미생물학적 활성에 관한 연구를 수행하였다. 식품 부패 및 식중독균에 대한 억제 효과는 0.4% 한천올리고당은 첨가한 경우 포노상구균과 대장균 O157:H7에 대하여 항균활성을 나타내었다. 한천올리고당은 pH의 변화에 매우 안정하여 pH는 항균활성에 영향을 주지 않았다. 또한 가압멸균할 경우 항균효과가 현저히 증가함을 보였다 한천올리고당의 가공 적성을 알아본 결과 아미노산 중 alanine, lysine, glycine, phenylalanine을 혼합하면 항균 활성이 증가함을 알 수 있었다

• 한국식품저장유통학회지
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• 제6권1호
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• pp.104-109
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• 1999

Ethyl acetate, methanol, water extract and their fractions from chestnut flower(Castancea crenata) were tested for antimicrobial activities. Yields of prebloomed chestnut flower extracts were 13.84, 12.90 and 1.82% in methanol, water and ethyl acetate, and those of the postbloomed were 13.12, 11.75 and 1.18%, respectively. Methanol extract from the chestnut flower was fractionated by solvents using hexane, chloroform, ethyl acetate, n-butanol and water, yields of those fractions were 0.16, 0.08, 1.94, 4.75 and 6.91% in the prebloomed, and were 0.90, 0.13, 1.40, 3.42 and 7.18% in the postbloomed. In the solvent extracts of water, ethyl acetate and methanol, methanol extract showed the most effective antimicrobial activity, antimicrobial activity of ethyl acetate fraction of methanol extract was stronger than others. Minimum inhibitory concentration of ethyl acetate fractions from the prebloomed showed 100, 140, 100 and 90ppm against Staphylococcus aureus, Streptococcus mutans, Echerichia coli and Salmonella typhimurium, in fractions from the postbloomed were 140, 140, 100 and 150ppm, respectively. Growth of all the strains was completely inhibited to 30 hours in a 150ppm concentration. E. coli sells treated with ethyl acetate fraction was collapsed severely.

• Natural Product Sciences
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• 제17권4호
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• pp.267-272
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• 2011

In our search for bioactive phenolics from plants, the culms of Phyllostachys bambusoides has been selected for investigation of anti-cariogenic and 1,1-diphenylpicrylhydrazyl (DPPH) radical scavenging agents based on the initial screening results. Fractionation process of n-hexane and $CHCl_3$ extracts afforded four phenolic constituents, ferulic acid (1), vanillin (2), coniferaldehyde (3), and coniferyl alcohol (4) as guided by their DPPH free radical scavenging activities. Additionally, activity-guided fractionation of EtOAc extract with anti-cariogenic activity has resulted in the isolation of coniferaldehyde (3), 2,6-dimethoxy-p-benzoquinone (5), p-methoxycinnamic acid (6), (${\pm}$)-balanophonin (7), and 6-methoxychromanone (8). The structures of 1 - 8 were determined by spectroscopic data interpretation, and also by comparison of their data with the published values. Phenolic compounds 1 - 4 exhibited similar DPPH radical scavenging activities compared with the synthetic antioxidant, butylated hydroxytoluene (BHT), and compounds 3 and 5 - 8 showed significant antibacterial activity against cariogenic oral streptococci, Streptococcus mutans and S. sobrinus.

• The Plant Pathology Journal
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• 제16권5호
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• pp.286-289
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• 2000

Mechanol extracts of three cold-tolerant eucalyptus trees-Eucalyptus darlympleana, E. gunnii and E. unigera were screened for antimicrobial activity against twenty two phyto-pathogenic fungi and six food-borne bacterial pathogens. E. unigera showed the antagonistic activity against all the tested pathogens. Among the tested fungal pathogens, Pythium species were highly sensitive to the leaf extracts. Especially, P. vanterpoolii, a causal agent of leaf blight in creeping bentgrass (Agrostis palustris), was completely inhibited by the extracts. The eucalyptus extracts were also effective in inhibiting the fungal growth of Botrytis cinerea and Phomopsis sp. isolated from the lesions of kiwifruit soft rot during post-harvest storage. Escherichia coli O-157 was less sensitive to the inhibition than the other bacterial pathogens tested. It was likely that Gram positive bacteria-Bacillus subtilis and Streptococcus mutans were more sensitive to the eucalyptus extracts than Gram negative bacteria-Escherichia coli, Salmonella enteritidis and Pseudomonas aeruginosa. Our findings suggest that the cold-tolerant eucalyptus species have antimicrobial properties that can serve the development of novel fungitoxic agents or food preservatives.

• International Journal of Oral Biology
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• 제39권4호
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• pp.187-192
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• 2014

Nonthermal atmospheric pressure plasma has attracted great interest for biomedical applications. The plasma consists of charged particles, radicals, and a strong electric field as the fourth state of matter. This study evaluated the change in the surface roughness after tooth bleaching by plasma in combination with a low concentration (15%) of carbamide peroxide, specifically whether the application of plasma produced detriments, such as demineralization and structural change, with the goal of efficient and safe tooth bleaching. After being combined with plasma and 15% carbamide peroxide, the hydroxyapatite surface was significantly smoother with a low roughness average value. Tooth bleaching with 15% carbamide peroxide alone produced an irregular surface and increased the surface roughness with high roughness average value. Tooth bleaching with plasma resulted in no significant variations in hydroxyapatite in terms of change in surface roughness and surface topography. The application of tooth bleaching with plasma is not deleterious to dental hard tissue, implicating it as a safe tooth bleaching technique.

• Journal of Microbiology and Biotechnology
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• 제14권4호
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• pp.759-765
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• 2004

The NCS inhibited the activity of glucosyltransferase which was produced by Streptococcus mutans JC-2, and the rate of inhibition at $100\muM<$ and $200\muM$ were 74.0% and 99.8%, respectively. It was stable in alkali condition, but unstable in acid condition. It was also stable up to $80^{\circ}C$. The kinetic study of the inhibition by NCS was carried out by Lineweaver-Burk plot and Dixon plot. It was non-competitive inhibition, determined by the two plots and $K_i$ and $K_i$ values were $15\muM$ and $19.3\muM$ respectively. The NCS did not show cytotoxicity against human gingival cells at $K_i$ ($15\muM$, $150\muM$, $1,500\mu$ M) concentrations. It had less cytotoxicity than chlohexidin, which has usually been used as the agent of anticaries. To evaluate the industrial applicability of the NCS, human pluck tooth was used. The inhibitory rates of tooth calcification and calcium ion elution by the NCS were 41 % and 2.5 times, respectively. These results suggested that NCS from Bacillus sp. S-1013 is an efficient anticaries agent.

• 한국식품영양학회지
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• 제25권2호
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• pp.284-290
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• 2012

물(water, TW), 에탄올(ethanol, TE) 및 핵산(n-hexane, TH)을 이용하여 탱자씨 추출물(trifoliate orange seed extracts, TSEs)을 각각 제조한 후 그람양성 식중독균 6종(Bacillus cereus KCCM 11341, Bacillus subtilis KCTC 1022, Listeria monocytogenes ATCC 12692, Staphylococcus aureus ATCC 19111, Streptococcus mutans KCTC 3065 및 Yersinia enterocolitica KCCM 41657)에 대한 항균활성과 Lactobacillus acidophilus IFO 3025에 대한 프레바이오틱 효과(prebiotic effect)를 측정하였다. 그 결과, 탱자씨 핵산추출물(TH)은 S. aureus ATCC 19111에 대해 배양시간이 증가함에 따라 대조군에 비해 강한 증식저해활성을 보였으며, 탱자씨 에탄올 추출물(TE)은 약간의 증식저해활성을 보였다. 특히, 배양 81시간 후 대조군은 90.31%의 증식활성을 보인 것에 반해 탱자씨 핵산추출물과 에탄올추출물은 64.95%와 75.50%의 증식활성을 각각 보였다. 탱자씨 추출물의 Lb. acidophilus IFO 3025에 대한 프레바이오틱 효과는 대조군에 비해 증식활성을 보이지는 않았으나, 적어도 생육저해효과를 보이지는 않았다. 따라서 탱자씨의 핵산 및 에탄올 추출물은 S. aureus ATCC 19111에 대한 항균활성물질로서의 가능성을 제시하였다.

• 치위생과학회지
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• 제4권1호
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• pp.45-48
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• 2004

In the current research for natural products with antimicrobial effects, various extracts of Puerariae thunbergiana and isoflavones against microorganisms were evaluated in terms of the minimum inhibitory concentrations (MIC). In general, Candida albicans was stronger antimicrobial activity than the other microorganisms such as Streptococcus mutans, Staphylococcus epidermidis, and Staphylococcus aureus. The maximum activity was exhibited by methanol extract of the leaves of Puerariae thunbergiana Beth. against Candida albicans(MIC, $400{\mu}g/mL$). These results suggest that methanol extract of Puerariae thunbergiana has a potential antimicrobial activity.