• KSBB Journal
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• 제18권6호
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• pp.500-505
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• 2003

재조합 대장균에서 내포체 형태로 발현시킨 LK68을 생물학적 활성을 가진 native한 단백질로 재생시키기 위해서 PBA 크로마토그래피와 EBA 크로마토그래피를 이용한 고체상 재접힘을 수행하였다. 내포체와 세포파쇄액을 시작물질로 하여 재접힘 공정을 수행하였으며 총 단백질 회수율과 재접힘 수율을 비교한 결과, EBA 공정이 기존의 액상 재접힘이나 PBA를 이용한 재접힘 공정에 비하여 우수함을 확인하였다. 또한 Iysine binding, RP-HPLC, SEC-HPLC, Ellman method 등을 사용하여 분석한 결과 재접힘된 LK68이 native LK68와 동등함을 확인하였다. 본 연구를 통해 EBA 크로마토그래피를 이용한 재접힘 방법은 재접힘 단계의 수율을 향상시킬 뿐 아니라 공정 단계, 시간 등을 감소시켜 공정 성능을 전체적으로 향상시킬 수 있음을 제시하였다.

• 통상정보연구
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• 제1권1호
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• pp.67-86
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• 1999

As we increase installing computers, traditional commerce by paper document is replaced by Electronic Commerce, paperless document commerce. Electronic Commerce proves to be an intermediary for increasing efficiency on work, which makes us use it more. This study aims to contribute to streamline local export by analyzing trade firms and manufacturers' using status of Electronic Commerce on work in Pusan. I sent questionnaires to 202 firms working on trade in Pusan to do survey. The sample of this study consists of 96 of them. I analyze using status of Electronic Commerce on work based on those data. The result shows that the more the firms install facility to be able for Electronic Commerce and the better they understand Electronic Commerce related laws, the more they select that on work. Generally The firms in Pusan rarely select Electronic Commerce on work and even acknowledges how much that is needed on work. Considering all of them, this study shows the method of streamlining local export by Electronic Commerce as follows; First, all kinds of Electronic Commerce such as trade related site in Internet should be systemized. Second, the laws on Electronic Commerce should be established in details. Third, workers on firms should be educated on Electronic Commerce. Forth, the firms should install the facility for Electronic Commerce, assist financially and make marketing strategy for selecting Electronic Commerce on each step in work. In conclusion, export in Pusan would be streamlined when the use of Electronic Commerce, effective intermediary, is motivated and we take that method to use on work properly.

• 대한기계학회논문집B
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• 제39권4호
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• pp.335-341
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• 2015

본 연구에서는 채널 벽면에 돌출물이 일정 간격으로 부착되어 있는 마이크로채널을 통과하는 2차원 스톡스 유동을 이론적으로 고찰한다. 상하 벽면에 부착된 돌출물들은 모두 반원 형상이고 서로 동일한 위상에 위치한다. 채널 내 유동의 주기성과 대칭성을 고려한 고유함수 전개법과 오차의 최소제곱법을 사용하여 유동장을 해석하여 유동함수 및 압력분포를 구하였다. 돌출물의 반경과 간격의 몇 가지 값들에 대하여 유동장 내의 유선 및 압력분포도를 보이고, 경계벽면에서의 전단응력 분포 등을 도시하였다. 또한, 돌출물의 반경과 돌출물 사이의 간격 변화에 따른 마이크로채널 내 평균 압력강하율의 변화를 계산하여 그림으로 나타내었다. 특히 상하 돌출물 사이의 틈새가 매우 작은 경우, 그 계산결과는 윤활이론의 결과와 아주 잘 일치함을 확인하였다.

• 한국소음진동공학회논문집
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• 제22권8호
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• pp.777-783
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• 2012

진동 인텐시티는 진동시스템에서 진동원의 위치를 찾는데 사용되어 왔다. 벡터 표현법 사용에 의해 파워흐름의 원인과 진동에너지 전달경로가 밝혀질 수 있다. 그러나, 판과 같은 구조물의 넓은 면적으로 인해 벡터 가시화를 사용하여 명확한 전달경로를 알아낼 수 없었다. 실험적으로 큰 면적의 물체에서는 측정점의 수가 늘어나게 된다. 이것은 측정에 많은 시간이 요구된다. 이번 연구에서는 FEM과 실험에 의한 모든 면에서 파워흐름 전달경로를 분명하게 가리키기 위해 유선 표현법이 사용되었다. 진동 인텐시티 전달경로를 분명하게 향상시키기 위해 실험과 FEM으로부터의 유선 표현을 비교하였다. 또한 FEM과 실험의 개선된 전달경로 가시화를 기존의 벡터표현과 비교하였다. 이 유선 가시화는 큰 표면을 갖는 판과 같은 구조물에 대해 진동원과 상세한 에너지 전달경로를 확인하는데 유용하다. 그 뿐만 아니라, 이 가시화 방법은 실험과 FEM 해석에 대해 많은 측정점을 필요로 하지 않는다.

• 한국생활과학회지
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• 제23권3호
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• pp.515-527
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• 2014

This study is intended to develop the creative and high value-added products as well as the development of diversity for jump suit for the future by analyzing the trend and feature shown in jump suit in the modern fashion. In the research methodology, the analysis was carried out over a total of 351 work pieces on jump suit among those presented in the collection of Paris, Milan, New York and London from 2006S/S to 2013F/W as well as literature review. The aesthetic features on suit jump design introduced in the modern fashion could be characterized as the following. First, both upper and lower garments are composed with a simple array of items and the stress was put on modernity feature through minimal expression technique. The feature of solid simplicity was also given with achromatic color or neutral monochrome. Second, the feminity image was emphasized with adoption of such highlighting items as detailed add-ons, tops, camisoles and blouses that stress the organically curved streamline including silhouette, material itself, crease and drape that enable the direct and indirect exposition of human body and the expression of smooth curve in human body. Third, jump suit revealed the multipurpose feature as item available for the diverse wear such as working habiliment, sports wear, uniform, office wear and evening wear, depending on the terms and conditions. Fourth, the deconstructive characteristic appeared through integration with various items, destruction of formative structure, non-structural shape, and ambiguity in wearing method.

• KIEAE Journal
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• 제13권2호
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• pp.67-76
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• 2013

The purpose of this study is to analyze the accessibility to the place for outdoor exercise. For that, the departure (resident) location of the users of outdoor exercise equipment within 12 parks in Seoul have been investigated. A total of 1,733 people have been surveyed during the two-week period between Aug.2 and Aug. 15 and 815 cases were analyzed. We also investigated demographic characteristics, access method and related factors such as location, climate and visiting time. The results were coded through ArcGis program and the accessibility to each exercise location, difference in regional accessibility and accessibility characteristics in terms of climate and time have been analyzed. The results are as follows: First, visitors to the Han River start from the places further than the visitors to the streamlines. Second, the average moving distance is longer for the users aged under-60 than for the users aged 60 and over to reach the exercise place. And the average moving distance for visitors to the Han River destination is the longest with 748 meters, followed by 203 meters for general parks and 92 meters for streamline parks, respectively. Third, in each non-waterfront parks, the moving distance by users under-60 was longer by about 230 meters than that by users 60 and over. Fourth, exercisers in the weekend travel more by 244 meters than those during the midweek users to reach the exercise place. Fifth, the number of visitors to the Han River increased in the weekend while visitors to the streamlines decreased in the same period. Sixth, the traveling length for bicycle users is three times as much as that for pedestrian visitors. And the departure locations of bicycle users were more widely distributed.

• 대한조선학회논문집
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• 제48권3호
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• pp.222-232
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• 2011

This article examines the scale effect of the flow characteristics, resistance and propulsion performance on a 317k VLCC. The turbulent flows around a ship in both towing and self-propulsion conditions are analyzed by solving the Reynolds-averaged Navier-Stokes equation together with the application of Reynolds stress turbulence model. The computations are carried out in both model- and full-scale. A double-body model is applied for the treatment of free surface. An asymmetric body-force propeller is used. The speed performances including resistance and propulsion factors are obtained from two kinds of methods. One is to analyze the computational results in model scale through the revised ITTC' 78 method. The other is directly to analyze the computational results in full scale. Based on the computational predictions, scale effects of the resistance and the self-propulsion factors including form factor, thrust deduction fraction, effective wake fraction and various efficiencies are investigated. Scale effects of the streamline pattern, hull pressure and local flow characteristics including x-constant sections, propeller and center plane, and transom region are also investigated. This study presents a useful tool to hull-form and propeller designers, and towing-tank experimenters to take the scale effect into consideration.

• 한국실내디자인학회논문집
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• 제15권6호
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• pp.19-26
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• 2006

This subject is about the research of Shanghai Art Deco in the aspect of architectural decoration and seek to offers an examination and analysis of the characteristics related to the Art Deco design, which appeared and dominated the field of design from 1910s to 1904s in Shanghai. Study method is a theoretical consideration of reference and gathering data through field trip. In order to carry out this subject, this study will research following factors. First, factors related to Art Deco such as the period of origin, background, spirit, aesthetics, design characteristics. This is followed by the influence of Art Deco movement begun in 1925. Second, the background of Shanghai Art Deco architecture style was studied. Third, the case studies of Shanghai Art Deco Architecture and interior decoration were researched by the expressive factors which were divided by style, form, material and color. As a result early style had compradore style about 1900, later reactionism prevailed of 1920 affected by European Art Deco. Skyscraper style was built and architecture developed with racial characteristics. It has vertical and streamline form of typical Art Deco and strengthen geometrical motive in architectural expression. It appeared naturally and has the contrast of different material in material expression. It has effect on strong color as using highborn and brilliant color in color expression. They appeared chinese national spirit by using 'ot painting' in western oriented furniture. The purpose of such an examination is to classify, understand and validate Shanghai artistic and socio-cultural heritage in order to better appreciate the life philosophy of Shanghai and re-discover their basic roots. Though it began as a Western cultural movement, it is the purpose of this study to discover the inherent orientalism in its basic formative spirit.

• 한국추진공학회지
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• 제24권1호
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• pp.34-46
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• 2020

직사각형 초음속 노즐의 3차원 역유동 추력벡터 제어 시스템에 대한 공기역학적 특성을 조사하기 위하여 수치해석을 수행하였다. 이 초음속 노즐은 특성곡선법에 의하여 설계되었으며, 그 설계 마하수는 2.5이다. 2차 유동 덕트의 갭 높이를 변수로 하여 역유동 추력벡터 제어 시스템의 성능을 조사하였다. 상부 흡입 칼라의 중심선을 따르는 정압 분포, 편향각, 2차 질량유량비 및 합성 추력계수와 같은 주요 매개변수가 정량적으로 분석되었다. 또한 전체 유동장의 특성을 알아보기 위하여 대칭 평면에서의 유선, 3차원 등마하수분포 및 3차원 난류에너지분포를 조사하였다.

• 한국추진공학회지
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• 제9권2호
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• pp.97-104
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• 2005

많은 장점에도 불구하고 유동함수를 이용한 수치해석용 격자생성 좌표변환기법의 단점은 저속영역에서의 격자간격이 고속영역에 비해 상대적으로 큼에 따라 수치적 처리에 많은 오차를 내포하고 있다는 점이다. 본 연구에서는 이러한 저속영역에서의 단점을 보완하기 위하여 격자간격을 속도크기 및 영역에 따라 적절히 조절할 수 있도록 수학적으로 변형된 압축성 유동함수를 이용한 좌표변환기법을 제안하고 가스터빈엔진에 주로 적용되는 유동모델로서 동심원상 두개 이상의 난류제트혼합유동에 대해 적용하였으며 해당 실험치, 즉 축 방향 평균속도분포, 난류운동에너지, 그리고 난류전단응력분포와 비교하여 난류운동에너지가 약간 과소평가 된 대칭축을 제외한 혼합경계층 내에서 $3.5\%$ 이내의 신뢰성을 확보하였다. 본 기법은 특히 터보팬엔진에 대한 내부흐름들의 혼합유동을 규명하거나 또는 난류전단응력에 의한 제트소음발생 및 저감방법을 도모하는데 유용하게 활용될 것으로 기대된다.