• Title/Summary/Keyword: streamline method

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Solid-Phase Refolding of Inclusion Body Protein in Packed Bed Adsorption and Expanded Bed Adsorption Chromatography (Packed Bed Adsorption과 Expanded Bed Adsorption 크로마토그래피를 이용한 내포체 단백질의 고체상 재접힘)

  • 최원찬;김민영;서창우;이은규
    • KSBB Journal
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    • v.18 no.6
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    • pp.500-505
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    • 2003
  • ‘LK (lipoprotein kringle) 68’is a polypeptide of a modified ansiostatin consisting of three kringle structures that might be clinically useful as a potential cancer therapeutics. It can be produced by overexpressing it as inclusion body in recombinant E. coli. In this study, solid-phase refolding processes using packed bed adsorption (PBA) and expanded bed adsorption (EBA) column were carried out to compare their refolding yields with that of the conventional, solution-phase refolding process, For the solution-phase and the PBA-mediated processes employing Q-Sepharose, washed inclusion body was used as the starting material, whereas both washed inclusion body and E. coli homogenate were used for the EBA-mediated process employing streamline DEAE. On the final recovery LK68 per unit mass of wet cell basis, the EBA- and PBA-mediated processes showed about 2.7- and 1.5-fold higher yields, respectively, than the solution-phase refolding method. The solid-phase refolded LK68 demonstrated the same Iysine binding bioactivity and the retention time in the RP-and SEC-HPLC as those of the native protein.

A Study of the Conditions on applying Electronic Commerce to the Process of Export by the Firms in Pusan (부산지역(釜山地域) 수출기업(輸出企業)의 전자상거래(電子商去來) 활용실태(活用實態)에 관한 고찰(考察))

  • Oh, Hyun-Soon;Yun, Kwang-Woon
    • International Commerce and Information Review
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    • v.1 no.1
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    • pp.67-86
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    • 1999
  • As we increase installing computers, traditional commerce by paper document is replaced by Electronic Commerce, paperless document commerce. Electronic Commerce proves to be an intermediary for increasing efficiency on work, which makes us use it more. This study aims to contribute to streamline local export by analyzing trade firms and manufacturers' using status of Electronic Commerce on work in Pusan. I sent questionnaires to 202 firms working on trade in Pusan to do survey. The sample of this study consists of 96 of them. I analyze using status of Electronic Commerce on work based on those data. The result shows that the more the firms install facility to be able for Electronic Commerce and the better they understand Electronic Commerce related laws, the more they select that on work. Generally The firms in Pusan rarely select Electronic Commerce on work and even acknowledges how much that is needed on work. Considering all of them, this study shows the method of streamlining local export by Electronic Commerce as follows; First, all kinds of Electronic Commerce such as trade related site in Internet should be systemized. Second, the laws on Electronic Commerce should be established in details. Third, workers on firms should be educated on Electronic Commerce. Forth, the firms should install the facility for Electronic Commerce, assist financially and make marketing strategy for selecting Electronic Commerce on each step in work. In conclusion, export in Pusan would be streamlined when the use of Electronic Commerce, effective intermediary, is motivated and we take that method to use on work properly.

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Stokes Flow Through a Microchannel with Projections of Constant Spacing (일정 간격의 돌출부를 갖는 마이크로채널 내의 스톡스 유동 해석)

  • Son, JeongSu;Jeong, Jae-Tack
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.39 no.4
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    • pp.335-341
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    • 2015
  • In this study, we analyzed a two-dimensional Stokes flow through a microchannel containing projections with constant spacing attached to each wall. The projections on the top and bottom walls were semi-circular in shape, with in-phase locations. By considering the periodicity and symmetry of the flow, the eigenfunction expansion and least squared error method were applied to determine the stream function and pressure distribution. For some typical radius and spacing values, the streamline patterns and pressure distributions in the flow field are shown, and the shear stress distributions on the boundary walls are plotted. In addition, the average pressure gradients in the microchannel are also calculated and shown with the radius and spacing of the projections. In particular, the results for the case of extremely small gaps between the projections on the top and bottom walls are in good agreement with the lubrication results.

Improved Vibration Vector Intensity Field for FEM and Experimental Vibrating Plate Using Streamlines Visualization (유선 가시화를 이용한 FEM과 실험에 의한 진동판에 대한 개선된 진동 벡터 인텐시티장)

  • Fawazi, Noor;Jeong, Jae-Eun;Oh, Jae-Eung
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.22 no.8
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    • pp.777-783
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    • 2012
  • Vibration intensity has been used to identify the location of a vibration source in a vibrating system. By using vectors representation, the source of the power flow and the vibration energy transmission paths can be revealed. However, due to the large surface area of a plate-like structure, clear transmission paths cannot be achieved using the vectors representation. Experimentally, for a large surface object, the number of measured points will also be increased. This requires a lot of time for measurement. In this study, streamlines representation is used to clearly indicate the power flow transmission paths at all surface plate for FEM and experiment. To clearly improve the vibration intensity transmission paths, streamlines representation from experimental works and FEM computations are compared. Improved transmission paths visualization for both FEM and experiment are shown in comparison to conventional vectors representation. These streamlines visualization is useful to clearly identify vibration source and detail energy transmission paths especially for large surface plate-like structures. Not only that, this visualization does not need many measured point either for experiment or FEM analysis.

A Study on the Plasticity and Characteristics on Jump Suit Shown in the Modern Fashion (현대패션에 나타난 점프 슈트(Jump Suit)의 조형성과 특성)

  • Kim, Sun Young
    • Korean Journal of Human Ecology
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    • v.23 no.3
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    • pp.515-527
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    • 2014
  • This study is intended to develop the creative and high value-added products as well as the development of diversity for jump suit for the future by analyzing the trend and feature shown in jump suit in the modern fashion. In the research methodology, the analysis was carried out over a total of 351 work pieces on jump suit among those presented in the collection of Paris, Milan, New York and London from 2006S/S to 2013F/W as well as literature review. The aesthetic features on suit jump design introduced in the modern fashion could be characterized as the following. First, both upper and lower garments are composed with a simple array of items and the stress was put on modernity feature through minimal expression technique. The feature of solid simplicity was also given with achromatic color or neutral monochrome. Second, the feminity image was emphasized with adoption of such highlighting items as detailed add-ons, tops, camisoles and blouses that stress the organically curved streamline including silhouette, material itself, crease and drape that enable the direct and indirect exposition of human body and the expression of smooth curve in human body. Third, jump suit revealed the multipurpose feature as item available for the diverse wear such as working habiliment, sports wear, uniform, office wear and evening wear, depending on the terms and conditions. Fourth, the deconstructive characteristic appeared through integration with various items, destruction of formative structure, non-structural shape, and ambiguity in wearing method.

Characteristics of Citizens' Access to Outdoor Exercise Places by Using the GIS - Focused on the Users of the Outdoor Exercise Equipment Installed in Parks, Seoul - (GIS를 이용한 도시민의 야외 운동장소 접근 특성 분석 - 서울시 공원의 운동기구 사용자를 중심으로 -)

  • Lee, Yeunsook;Gu, Naeun;Lee, Dongjoo
    • KIEAE Journal
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    • v.13 no.2
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    • pp.67-76
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    • 2013
  • The purpose of this study is to analyze the accessibility to the place for outdoor exercise. For that, the departure (resident) location of the users of outdoor exercise equipment within 12 parks in Seoul have been investigated. A total of 1,733 people have been surveyed during the two-week period between Aug.2 and Aug. 15 and 815 cases were analyzed. We also investigated demographic characteristics, access method and related factors such as location, climate and visiting time. The results were coded through ArcGis program and the accessibility to each exercise location, difference in regional accessibility and accessibility characteristics in terms of climate and time have been analyzed. The results are as follows: First, visitors to the Han River start from the places further than the visitors to the streamlines. Second, the average moving distance is longer for the users aged under-60 than for the users aged 60 and over to reach the exercise place. And the average moving distance for visitors to the Han River destination is the longest with 748 meters, followed by 203 meters for general parks and 92 meters for streamline parks, respectively. Third, in each non-waterfront parks, the moving distance by users under-60 was longer by about 230 meters than that by users 60 and over. Fourth, exercisers in the weekend travel more by 244 meters than those during the midweek users to reach the exercise place. Fifth, the number of visitors to the Han River increased in the weekend while visitors to the streamlines decreased in the same period. Sixth, the traveling length for bicycle users is three times as much as that for pedestrian visitors. And the departure locations of bicycle users were more widely distributed.

Computational Study of the Scale Effect on Resistance and Propulsion Performance of VLCC (대형 유조선의 저항 및 추진성능에 대한 축척효과의 수치적 연구)

  • Choi, Jung-Eun;Kim, Jung-Hun;Lee, Hong-Gi
    • Journal of the Society of Naval Architects of Korea
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    • v.48 no.3
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    • pp.222-232
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    • 2011
  • This article examines the scale effect of the flow characteristics, resistance and propulsion performance on a 317k VLCC. The turbulent flows around a ship in both towing and self-propulsion conditions are analyzed by solving the Reynolds-averaged Navier-Stokes equation together with the application of Reynolds stress turbulence model. The computations are carried out in both model- and full-scale. A double-body model is applied for the treatment of free surface. An asymmetric body-force propeller is used. The speed performances including resistance and propulsion factors are obtained from two kinds of methods. One is to analyze the computational results in model scale through the revised ITTC' 78 method. The other is directly to analyze the computational results in full scale. Based on the computational predictions, scale effects of the resistance and the self-propulsion factors including form factor, thrust deduction fraction, effective wake fraction and various efficiencies are investigated. Scale effects of the streamline pattern, hull pressure and local flow characteristics including x-constant sections, propeller and center plane, and transom region are also investigated. This study presents a useful tool to hull-form and propeller designers, and towing-tank experimenters to take the scale effect into consideration.

A Research of Shanghai Art Deco in the aspect of architectural decoration (건축 의장적 측면에서 본 상하이 아르테코에 관한 연구)

  • Nam, Kyung-Sook;Suh, Min-Won
    • Korean Institute of Interior Design Journal
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    • v.15 no.6 s.59
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    • pp.19-26
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    • 2006
  • This subject is about the research of Shanghai Art Deco in the aspect of architectural decoration and seek to offers an examination and analysis of the characteristics related to the Art Deco design, which appeared and dominated the field of design from 1910s to 1904s in Shanghai. Study method is a theoretical consideration of reference and gathering data through field trip. In order to carry out this subject, this study will research following factors. First, factors related to Art Deco such as the period of origin, background, spirit, aesthetics, design characteristics. This is followed by the influence of Art Deco movement begun in 1925. Second, the background of Shanghai Art Deco architecture style was studied. Third, the case studies of Shanghai Art Deco Architecture and interior decoration were researched by the expressive factors which were divided by style, form, material and color. As a result early style had compradore style about 1900, later reactionism prevailed of 1920 affected by European Art Deco. Skyscraper style was built and architecture developed with racial characteristics. It has vertical and streamline form of typical Art Deco and strengthen geometrical motive in architectural expression. It appeared naturally and has the contrast of different material in material expression. It has effect on strong color as using highborn and brilliant color in color expression. They appeared chinese national spirit by using 'ot painting' in western oriented furniture. The purpose of such an examination is to classify, understand and validate Shanghai artistic and socio-cultural heritage in order to better appreciate the life philosophy of Shanghai and re-discover their basic roots. Though it began as a Western cultural movement, it is the purpose of this study to discover the inherent orientalism in its basic formative spirit.

Assessment of the Counter-Flow Thrust Vector Control in a Three-Dimensional Rectangular Nozzle (3차원 직사각형 노즐에서 역유동 추력벡터 제어 평가)

  • Wu, Kexin;Kim, Tae Ho;Kochupulickal, James Jintu;Kim, Heuy Dong
    • Journal of the Korean Society of Propulsion Engineers
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    • v.24 no.1
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    • pp.34-46
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    • 2020
  • Computational assessment of gas-dynamic characteristics is explored for a three-dimensional counter-flow thrust vector control system in a rectangular supersonic nozzle. This convergent-divergent nozzle is designed by Method of Characteristics and its design Mach number is specially set as 2.5. Performance variations of the counter-flow vector system are illustrated by varying the gap height of the secondary flow duct. Key parameters are quantitatively analyzed, such as static pressure distribution along the centerline of the upper suction collar, deflection angle, secondary mass flow ratio, and resultant thrust coefficient. Additionally, the streamline on the symmetry plane, three-dimensional iso-Mach number surface contour, and three-dimensional turbulent kinetic energy contour are presented to reveal overall flow-field characteristics in detail.

A Simple Calculational Method by using Modified Von Mises Transformation applied to the Coaxial Turbulent Jet Mixing (유동함수를 이용한 난류제트혼합유동 계산에 관한 연구)

  • Choi Dong-Whan
    • Journal of the Korean Society of Propulsion Engineers
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    • v.9 no.2
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    • pp.97-104
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    • 2005
  • A simple but efficient grid generation technique by using the modified compressible form of stream function has been formulated. Transformation of a physical plane to a streamline plane, the Von Mises Transformation, has been widely used to solve the differential equations governing flow phenomena, however, limitation arises in low velocity region of boundary layer, mixing layer and wake region where the relatively large grid spacing is inevitable. Modified Von Mises Transformation with simple mathematical adjustment for the stream function is suggested and applied to solve the confined coaxial turbulent jet mixing with simple $\kappa-\epsilon$ turbulence model. Comparison with several experimental data of axial mean velocity, turbulent kinetic energy, and Reynolds shear stress distribution shows quite good agreement in the mixing layer except in the centerline where the turbulent kinetic energy distributions were somewhat under estimated. This formulation is strongly suggested to be utilized specially for free turbulent mixing layers in axisymmetric flow conditions such as the investigation of mixing behavior, jet noise production and reduction for Turbofan engines.