• Journal of Microbiology
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• v.40 no.3
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• pp.237-240
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• 2002

A carboxydotrophic bacterium, isolated from a soil sample in Seoul, was classified initially as Acinetobacter sp. strain JC1 DSM 3803. Chemotaxanomic properties, analysis of the 16s rDNA sequence, fatty acid content, and molecular Phylogenetic analysis based on rpoB gene, however, suggested that this bacterium belongs to the genus, Mycobacterium. On the basis of this evidence, it is proposed that Acinetobacter sp. strain JC1 DSM 3803 be reclassified as Mycobacterium sp. strain JC1 DSM 3803.

• Mycobiology
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• v.47 no.2
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• pp.173-179
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• 2019

Cladophialophora is characterized by simple hyphomycetes with brown hyphae that give rise to branched chains of pale brown conidia and shows affinities with the Herpotrichiellaceae. A fungal strain belonging to the genus Cladophialophora was isolated from soil in Daegu, Korea. This strain produces numerous greenish to dark black lanose aerial mycelia with hair like structures. It is morphological similar to C. chaetospira, C. inabaensis, and C. multiseptata; however, the conidiophores and conidia sizes of the newly isolated strain (KNU16-032) are clearly different from them. The novelty of the strain was also confirmed based on phylogenetic analysis using the data sets of the internal transcribed spacer region of and the partial sequence of 28S ribosomal DNA region along with the cultural characteristics. Because morphological observations and phylogenetic analysis indicated that the strain is distinct from previously known Cladophialophora species, we propose this species as a new species Cladophialophora lanosa sp. nov., and provide the detailed descriptions in this study.

• Proceedings of the KSME Conference
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• 2001.11a
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• pp.99-104
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• 2001

Effect of mean stress on the fatigue life of natural rubber for engine mount was investigated. Fatigue damage parameter based on the maximum Green-Lagrange strain was employed to account for the effect of mean stress. A procedure to predict the fatigue life of rubber components based on the maximum Green-Lagrange strain method was proposed. Nonlinear finite element analysis and fatigue test of jang-gu shape specimen were conducted to predict the fatigue life of engine mount. Predicted fatigue lives have a good agreement with tested lives within a factor of 3.

• Proceedings of the KSME Conference
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• 2000.04a
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• pp.61-66
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• 2000

The fatigue crack initiation life is studied on automotive spot weldment made from cold rolled carbon steel(SPC) sheet by using DCPDM and local strain approach. It can be found that the fatigue crack initiation behavior in spot weldment can be definitely detected by DCPDM system. The local stresses and strains are estimated by elastic-plastic FEM analysis and the alternative approximate method based on Neuber's rule were applied to predict the fatigue life of spot weldment. A satisfactory correlation between the predicted life and experimental life can be found in spot weldment within a factor of 4.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.25 no.2
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• pp.220-227
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• 2001

The fatigue crack initiation life is studied on automotive tensile-shear spot weldment made from cold rolled carbon steel(SPC) sheet by using DCPDM and local strain approach. It can be found that the fatigue crack initiation behavior in spot weldment can be definitely detected by DCPDM system. To predict the fatigue life of spot weldment, the local stresses and strains at the potential critical region are estimated by approximate method based on Neubers rule and elastic-plastic FEM analysis. A satisfactory correlation between the predicted life obtained from Local strain approach based on Neubers rule and experimental life can be found in spot weldment within a factor of 2.

• Transactions of the Korean Society of Mechanical Engineers
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• v.14 no.5
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• pp.1349-1355
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• 1990

By examining the fatigue deformation properties of 12% Cr rotor steel which has been proved to have high fatigue and creep rupture strength around 600deg. C, authors reviewed major fatigue life prediction models such as Manson, Langer and Morrow equations, and following results were obtained. (1) A simple life prediction model for 12% Cr rotor steel was obtained as follows : DELTA..epsilon.$_{t}$ =2.18+.sigma.$_{u}$ /E+ $N^{-0.065}$+ $e^{0.6}$ $N^{-0.025}$ This equation shows that fatigue life, N, can be easily determined when total strain range, DELTA..epsilon.$_{t}$ and ultimate tensile strength, .sigma.$_{u}$ are known by simple tension test on the given test conditions. (2) Life prediction equation with equivalent maximum stress, DELTA..sigma./2, corresponding maximum strain in one cycle at room temperature is as follows: DELTA..sigma./w=-7.01logN+96.69+96.69

• The Journal of the Korea Contents Association
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• v.20 no.8
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• pp.251-261
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• 2020

This study aims to suggest a new perspective that can account for variations in fertility rates across OECD countries. Most previous literature has highlighted the influence of government policies on fertility rates. This study focuses the role of job strain, unequal division of household labor, and life satisfaction on fertility rates. These factors are related to work-life balance, and play a crucial role in understanding variations in fertility rates across OECD countries. Using fuzzy set qualitative comparative research analysis (fsQCA), this study tests whether fertility rates can be explained by differences in the levels of job strain, gender equality at home, and life satisfaction across countries. The results are as follows: First, high fertility-countries show low levels of job strain, equal division of household labor, high levels of life satisfaction, and high levels of GDP. Second, a high level of GDP is not crucial for achieving high fertility rates. This study suggests that changes in working conditions and organizational culture are required to increase the fertility rate in Korea, since this can influence work-life balance, life satisfaction and equal division of household labor.

• Journal of the Korean Society of Safety
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• v.36 no.3
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• pp.7-14
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• 2021

Hybrid laminated Al/carbon-fiber-reinforced plastic (CFRP) composites are attracting considerable attention from industries such as aerospace and automobiles owing to their excellent specific strength and specific rigidity. However, when this material is used to fabricate high-pressure fuel storage containers subjected to repeated fatigue loads, fatigue life evaluation for the working load is regulated as an important criterion for operational safety and ease of maintenance. Among the existing evaluation methods for these vessels, the burst test and the hydraulic repeat test require expensive facilities. Thus, the present study aims to develop an improved fatigue life test for Al/CFRP laminated hybrid composites. The test specimen was manufactured using a curved mold considering the shape of a type III high-pressure storage container. The strain-life method was used for fatigue life evaluation, and the life was predicted based on the transition life. The results indicate that the more complex the CFRP stacking sequence, the longer is the transition life. This test method is expected to be useful for ensuring the fatigue safety and economy of hybrid laminate composites.

• Journal of Microbiology and Biotechnology
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• v.19 no.10
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• pp.1223-1229
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• 2009

To construct a new recombinant strain of Bacillus velezensis that has antifungal and insecticidal activity via the expression of the insecticidal Bacillus thuringiensis crystal protein, a B. thuringiensis expression vector (pHT1K-1Ac) was generated that contained the B. thuringiensis cry1Ac gene under the control of its endogenous promoter in a minimal E. coli-B. thuringiensis shuttle vector (pHT1K). This vector was introduced into a B. velezensis isolate that showed high antifungal activities against several plant diseases, including rice blast (Magnaporthe grisea), rice sheath blight (Rhizotonia solani), tomato gray mold (Botrytis cinerea), tomato late blight (Phytophthora infestans), and wheat leaf rust (Puccinia recondita), by electroporation. The recombinant B. velezensis strain was confirmed by PCR using cry1Ac-specific primers. Additionally, the recombinant strain produced a protein approximately 130 kDa in size and parasporal inclusion bodies similar to B. thuringiensis. The in vivo antifungal activity assay demonstrated that the activity of the recombinant B. velezensis strain was maintained at the same level as that of wild-type B. velezensis. Furthermore, it exhibited high insecticidal activity against a lepidopteran pest, Plutella xylostella, although its activity was lower than that of a recombinant B. thuringiensis strain, whereas wild-type B. velezensis strain did not show any insecticidal activity. These results suggest that this recombinant B. velezensis strain can be used to control harmful insect pests and fungal diseases simultaneously in one crop.

• Journal of Mushroom
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• v.15 no.1
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• pp.8-13
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• 2017

An auxin-producing bacterial strain, designated 4-3, was isolated from waste button mushroom compost in Boryeong-si, Chungnam. The strain 4-3 was classified as a novel strain of Leucobacter tardus, based on chemotaxonomic and phylogenetic analyses. TLC and HPLC the isolated L. tardus strain 4-3 produced indole-3-acetic acid (IAA), the auxin. Maximum IAA productionof $94.3mg\;L^{-1}$ was detected for bacteria cultured in R2A medium with 0.1% l-tryptophan, incubated for 24 h at $35^{\circ}C$. Negative correlationwas observed between IAA production and pH of the culture medium, indicating that the increase inIAA caused acidification ofthe medium. The effect of supplementation with varying concentrations of l-tryptophan, a known precursor of IAA, was also assessed. production was maximal at 0.1% l, but decreased at lconcentrations above 0.2%. To investigate the plant growth-promoting effects of the bacterium, L. tardus strain 4-3 culture broth was used to inoculate water cultures and seed pots of mung bean. We found thatadventitious root induction and root growth were 2.2-times higher in thethan in the non-inoculated plants.