• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2003.10a
• /
• pp.103.1-103
• /
• 2003

An antagonistic bacteria, Stenotrophomonas maitophilia BW-13 strain which was effectively inhibited mycerial growth of Bottom rot pathogen, Rhizoctonia solani PY-1 strain was isolated from the rhizosphere of the lettuce in Uiryeong-Gun, Gyeongsangnam-Do from 2002 to 2003. For the biological control, the most suitable inoculum and its density of pathogen, PY-1 strain ware tested prior biological control test, For the pathogenicity test, A inoculum (wheat bran)sawdust+rice bran+PDB) showing disease incidence of 100％ was selected as the most suitable inoculum, which showed more effective than B inoculum (sawdust＋rice bran＋DW) and mycelial disc. also, In selection of the amount of inoculum (40g, 50g, 60g, 70g, 80g), most suitable amount of inoculum of pathogen determined as 40g showing disease incidence of 80％. For the selection of effective microorganism to control bottom rot on lettuce, about 200 isolates were isolated from the diseased soil and lettuce leaves, and examined their antifungal activity to the pathogen on PDA. As the pots assay, BW-13 strain showed the highest control value as 90％, and followed by R-13 and R-26 strain as 80％ and 60％, respectively. Selected BW-13 isolates identified as 5. maltophilia (GeneBank accession no. AJ293473.1, 99％) by 16S rRNA sequencing. This is the first report on the biological control using by S. maltophilia to the bottom rot pathogen, Rhizoctonia solani PY-1 strain.

• Journal of Microbiology and Biotechnology
• /
• v.20 no.2
• /
• pp.281-286
• /
• 2010

A Pseudomonas fluorescens strain was isolated and found to show antagonistic activity against phytopathogenic fungi and to possess a gene responsible for production of antibiotic 2,4-diacetylphloroglucinol. For the extension of biocontrol range, a gene for an Androetonus australis Hector insect toxin 1 (AaHIT1), one of the most known toxic insect-selective peptides, was designed and synthesized according to the preferred codon usage of Pseudomonas fluorescens, cloned, and transformed into the strain by pSUP106 vector, a broad-host-range plasmid. Bioassays indicated that the engineered strain was able to produce AaHIT1 with insecticidal activity, and at the same time retain the activity against plant pathogen. The experiments for nonplanted soil and rhizosphere colonization showed that, similar to the population of the wild-type strain, that of the engineered strain remained relatively constant in the first 10 days, and the subsequent 50 days, suggesting that AaHIT1 expression in the bacterial cell does not substantially impair its long-term colonization. It is first reported that a Pseudomonas fluorescens strain expressing an active scorpion neurotoxin has dual activity against phytopathogenic fungi and insects, making at attractive for agronomic applications.

• Journal of Welding and Joining
• /
• v.12 no.4
• /
• pp.151-161
• /
• 1994

FCAW has wide application in ship fabrication, maintenance and field erection. It has many advantages over SMAW.SAW and GMAW process. In many applications, the FCAW provides highquality weld metal. This method can reduce weld defects especially porosity and spatter. But the fatigue characteristics of those deposited metal have been rarely investigated. The purpose of this study is to investigate the cyclic stress-strain behavior and fatigue tests by the constant strain control were carried out on the rounded smooth specimen with deposited metal using the metal type flux cored wire. As the results of this study for the deposited metal welded by the metal type flux cored wire, the hardening or softening characteristics under cyclic load were investigated and cyclic stress-strain curve, strain-fatigue life curve, stress-strain function and fatigue life relation which are useful to estimate the fatigue life under the stress concentration condition were obtained.

• The Plant Pathology Journal
• /
• v.20 no.2
• /
• pp.115-126
• /
• 2004

The actinomycete strain PK-A41 was isolated from a soil sample from pepper fields in Ko-yang, Korea. The strain PK-A41 inhibited the mycelial growth of some plant pathogenic fungi and oomycete, Alternaria mali, Colletotrichum orbiculare, Fusarium oxysporum f.sp. lycopersici, Magnaporthe grisea, Rhizoctonia solani, and Phytophthora capsici. The presence of LL-diaminopi-melic acid in the cell wall extract and the nucleotide sequence of the 16S rDNA region of the strain PK-A41 was assigned to Streptomyces scabiei. Further morpho-logical, biochemical, and pathological analyses also confirmed the strain PK-A41 to be S. scabiei, which is pathogenic to potato tubers. The maximum antibiotic production of the strain PK-A41 was achieved when grown on the glycerol peptone broth (GPB) medium for 9 days.

• Journal of the Korean Society for Precision Engineering
• /
• v.11 no.4
• /
• pp.114-125
• /
• 1994

Fatigue behavior and life prediction method were presented for themal-mechanical and isothermal low cycle fatigue of 12 Cr forged steel used for high temperature applications. In-phase and out-of-phase thermal-mechanical fatigue test from 350 .deg. C to 600 .deg. C and isothermal low cycle fatigue test at 600 .deg. C, 475 .deg. C, 350 .deg. C were conducted using smooth cylindrical hollow specimen under strain-control with total strain ranges from 0.006 to 0.015. The phase difference between temperature and strain in thermal-mechanical fatigue resulted in significantly shorter fatigue life for out-of-phase than for in-phase. Thermal-mechanical fatigue life predication was made by partitioning the strain ranges of the hysteresis loops and the results of isothermal low cycle fatigue tests which were performed under the combination of slow and fast strain rates. Predicted fatigue lives for out-of-phase using the strain range partitioning method showed an excellent agreement with the actual out-of-phase thermal-mechanical fatigue lives within a factor of 1.5. Conventional strain range partitioning method exhibited a poor accuracy in the prediction of in-phase range partitioning method in a conservative way. By the way life prediction of thermal-mechanical fatigue by Taira's equivalent temperature method and spanning fartor method showed good agreement within out-of-phase thermal-mechanical fatigue.

• Transactions of Materials Processing
• /
• v.19 no.1
• /
• pp.11-16
• /
• 2010

The influence of pre-strain on low cycle fatigue behavior of Fe-18Mn-0.05Al-0.6C TWIP steel was studied by conducting axial strain-controlled tests. As-received plates were deformed by rolling with reduction ratios of 10 and 30%, respectively. A triangular waveform with a constant frequency of 1 Hz was employed for low cycle fatigue test at the total strain amplitudes in the range of ${\pm}0.4\;{\sim}\;{\pm}0.6$ pct. The results showed that low-cycle fatigue life was strongly dependent on the amount of pre-strain as well as the strain amplitude. Increasing the amount of prestrain, the number of reversals to failure was significantly decreased at high strain amplitudes, but the effect was negligible at low strain amplitudes. A new model for predicting fatigue life of pre-strained body has been suggested by adding ${\Delta}E_{pre-strain}$ to the energy-based fatigue damage parameter. Also, high-cycle fatigue lives predicted using the low-cycle fatigue data well agreed with the experimental ones.

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2003.10a
• /
• pp.102.2-103
• /
• 2003

This studies were investigated the occurrence of Sclerotinia rot by Sclerotinia Sclerotiorum at the lettuce field in Uiryeong-Gun, Gyeongsangnam-Do and were isolated the most effective microorganism for the biological control to the pathogen, S. sclerotiorum YR-1 strain from diseased soil and lettuce leaves. For the pathogenicity test, the most suitable inoculmn density of YR-1 strain was selected as the mycelial suspension of 40m1 showing disease incidence of 80％, and the symptom showed as same as at the fields, the leaves and stem had rotten and developed white downy mycelial at the diseased lesion on the leaves and stems, and produced black and irregular sclerotinia. On the PDA dual test, about 300 isolates were examined the antifungal activity to the pathogen, YR-1 strain, and among them, A-2, A-7, and RH-4 strain were selected most effective antagonistic bacteria. At pots test, the control value of A-7 strain showed the highest value as 85％ which was more effective than that of others in a growth chamber. For the promotion of control effect, the selected 3 isolates were spayed on the lettuce leaves as a sole and/or mixed treatments in a growth chamber, the mixed treatment of A-7 and RH-4 strain showing the control value of 90％ was most effective than that of sole treatment with A-7 or RH-4 strain showing the control value of 80％, respectively and mixed treatment with A-2 and A-7 strain and A-2, A-7 and RH-4 strain. In addition, 3 bacteria re-isolated from diseased soils, and all of the selected 6 isolates investigated the control effect at pots in a growth chamber, According to the results, A-7 and Pro-EB 15 strain showed the control value of 91.0％ and 90.1％ respectively, and they were selected most effectual antagonistic bacteria to control lettuce sclerotinia rot and identified as the Bacillus mojanuinensis by 16s RNA analysis. This is the first report on the biological control using by B. mojanvinensis to the lettuce Sclerotinia rot.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.26 no.3
• /
• pp.521-527
• /
• 2002

In this paper, tensile behavior and low cycle fatigue behavior of 316L stainless steel which is currently favored structural material for several high temperature components such as the liquid metal cooled fast breeder reactor (LMFBR) were investigated. Research was performed at 55$0^{\circ}C$, $600^{\circ}C$ and $650^{\circ}C$ since working temperature of 316L stainless steel in a real field is from 40$0^{\circ}C$ to $650^{\circ}C$. From tensile tests performed by strain controls with $1{\times}10^{-3}/s,\; l{\times}10^{ -4}/s \;and\; 1{\times}10/^{ -5}/ s $ strain rates at each temperature, negative strain rate response (that is, strain hardening decreases as strain rate increases) and negative temperature response were observed. Strain rate effect was relatively small compared with temperature effect. LCF tests with a constant total strain amplitude were performed by strain control with a high temperature extensometer at R.T, 55$0^{\circ}C$, $600^{\circ}C$, $650^{\circ}C$ and total strain amplitudes of 0.3%~0.8% were used and test strain rates were $1{times}10^{-2} /s,\; 1{times}10^{-3} /s\; and\; 1{times}10^{-4} /s$. A new energy based LCF life prediction model which can explain the effects of temperature, strain amplitude and strain rate on fatigue life was proposed and its excellency was verified by comparing with currently used models.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.21 no.2
• /
• pp.199-208
• /
• 1997

A local strain approach was applied to an external single and double grooved C-shaped specimen in order to evaluate and predict the fatigue crack initiation life by using low cycle fatigue properties. The low cycle fatigue properties were determined from the strain-controlled fatigue tests using smooth cylindrical axial specimens. Fatigue crack initiation life was evaluated by a life prediction software, FALIPS, based on the local strain approach. The fatigue life was significantly influenced by the mean stress, and SWT parameter represented the fatigue life effectively. The predicted fatigue crack initiation life was then compared to the experimental fatigue life evaluated from the C-shaped fatigue test specimens. A good correlation was found between the experimental and predicted fatigue lives within factors of 2 and 4 for the single and double grooved C-shaped specimens respectively. Also, experimental fatigue life of the double grooved specimen was 10-12 times longer than that of the single grooved specimen.

• Journal of the Korean Wood Science and Technology
• /
• v.50 no.5
• /
• pp.301-314
• /
• 2022

The moisture and dimensional behaviors of a nail-laminated timber (NLT)-concrete slab composed of an NLT-plywood composite and topping concrete are monitored for 385 days. The slab is developed for using as flexural elements such as floors. The humidity control of wood gently introduces significant fluctuations under the ambient relative humidity into the slab, and fluctuations in the relative humidity result in dimensional changes. The equilibrium moisture content of the slab increases from 6.7% to 15.3% during the monitoring period, resulting in a width (radial) strain of 0.58%. The length (longitudinal) strain is negligible, and the height (tangential) strain is excluded from the analysis because of abstruse signal patterns generated. Concrete pouring causes a permanent increase in the width of the NLT-plywood composite. However, the width deforms because the weight of the concrete mixture loosens the nail-laminated structure, not because of the significant amount of moisture in the mixture. The dimensional stabilization effect of the nail-laminated system is demonstrated as the composite strain is lower than the total strain of lumber and plywood, which are elements constituting the nail-laminated structure.