Browse > Article
http://dx.doi.org/10.5423/PPJ.2004.20.2.115

Isolation and Antifungal and Antioomycete Activity of Streptomyces scabiei Strain PK-A41, the Causal Agent of Common Scab Disease  

Han, Won-Choon (Laboratory of Molecular Plant Pathology, College of Life and Environmental Sciences, Korea University)
Lee, Jung-Yeop (Laboratory of Molecular Plant Pathology, College of Life and Environmental Sciences, Korea University)
Park, Duck-Hwan (Division of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University)
Lim, Chun-Keun (Division of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University)
Hwang, Byung-Kook (Laboratory of Molecular Plant Pathology, College of Life and Environmental Sciences, Korea University)
Publication Information
The Plant Pathology Journal / v.20, no.2, 2004 , pp. 115-126 More about this Journal
Abstract
The actinomycete strain PK-A41 was isolated from a soil sample from pepper fields in Ko-yang, Korea. The strain PK-A41 inhibited the mycelial growth of some plant pathogenic fungi and oomycete, Alternaria mali, Colletotrichum orbiculare, Fusarium oxysporum f.sp. lycopersici, Magnaporthe grisea, Rhizoctonia solani, and Phytophthora capsici. The presence of LL-diaminopi-melic acid in the cell wall extract and the nucleotide sequence of the 16S rDNA region of the strain PK-A41 was assigned to Streptomyces scabiei. Further morpho-logical, biochemical, and pathological analyses also confirmed the strain PK-A41 to be S. scabiei, which is pathogenic to potato tubers. The maximum antibiotic production of the strain PK-A41 was achieved when grown on the glycerol peptone broth (GPB) medium for 9 days.
Keywords
Streptomyces scabiei; antibiotic activity; identi-fication; culture condition;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Becker, B., Lechevalier, M. P. and Lechevalier, H. A. 1965. Chemical composition of cell wall preparations from strains of various form-genera of aerobic actinomycetes. Appl. Microbial. 13:236-243
2 Becker, B., Lechevalier, M. P., Gordon, R. E. and Lechevalier, H. A. 1964. Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole-cell hydrolysates Appl. Microbiol. 12:421-423
3 Elesaway, A, A. and Szabo, J. M. 1979. Isolation and characterization of Streptomyces scabies strairts from scab lesions of potato tubers. Designation of the nrotype strain of Streptomyces scabies. Acta Microbiol. Acad. Sci. Hung. 26:311-320
4 Einbley,T. M. 1994. The molecrtlar phylogeny and systemdtics of the Actinomycetes. Annu. Rev. Microbiol. 48:257-289   DOI   ScienceOn
5 Flower, T. H. and Williams, S. T. 1977. The influence of pH on the growth rate ahd viability of neutrophilic and acidophilic Streptomycetes.Miicrobios. 18:223-228
6 Goodfellow, M. and Williams, S. T. 1983. Ecology of actinomycetes. Ann. Rev. Microbial. 37:189-216   DOI   ScienceOn
7 KenKnight, G. 1941. Studies on soil actinomycetes in relation to potato scab and its control. Michigan Agr. Exp. Sta. Techn. Bull. 178
8 Sugawara, T., Tanaka, A., Imai, H., Magai, K. and Snzuki, K. 1997. YM-47515, a novel isonitrile antibiotic from Micronomospora echinospora subsp. echinospora. J. Antibiot. 50:944-948   DOI   ScienceOn
9 Taylor, L. F. and Decker, P. 1947. A correlation between pathogenicity and cultural characteristics in the genus Actinomyces. Phytopathology 37:49-58
10 Waksman, S. A. 1961. Classification, identification and descriptions of genera and species. In: The Actinomycetes. Vol. II. Waksman, S. A. ed. the Williams & Wikins Co., Baltimore. pp.1-363
11 Williams, S. T., Goodfellow, M., Wellington, E. M. H., Vickers, J. C, Alderson, G., Sneath, P. H. A, Sackin, M. J. and Mortimer, A. M. 1983b. A probability matrix for identification of Streptomycetes. J. Cell. Microbiol. 129:1815-1830
12 Zuckerkandl, E. and Pauling, L. 1965. Molecules as documents of evolutionary history. J. Theor. Biol. 8:357-366   DOI   ScienceOn
13 Williams, S. T., Sharpe, M. E., Holt, J. G., Murray, R .G. E., Brener, D. J., Krieg, N. R., Mouldar, J. W., Pfennig, N., Sneath, P. H. A .and Staley, J. T. 1989. Bergeys Manual of Systematic Bacteriology. Vol. 4. Williams & Wilkins, Baltimore
14 Locci, R. 1989. Strepto'mycetes and related genera. In: Bergeys Manual of Systermatic Bacteriology. Vol. 4. Williams & Wilkins, Baltimore. pp. 2451-2508
15 Schatz, A., Bugie, E. and Waksman, S. A. 1944. Stretpmycin, a substance exhibiting antibiotic activity against gram-positive and gram-negative bacteria. Proc. Soc. Exptl. BioI. Med. 55:66-69
16 Lechevalier, H. A. and Lechevalier, M. P. 1970. A critical evaluation of the genera of aerobic actinomycetes. In: The Actinomycetales. Prauser, H. ed., Gustav Fischer Verlag, Jena. pp. 393-405
17 Connell: N. D. 2001. Expression systems for use in actinomycetes and related organisms. Cur. Opin. Biotechnol. 12:446-449   DOI   PUBMED   ScienceOn
18 Heery, D. M., Gannon, F. and Powell, R. 1990. A simple method for subcloning DNA fragments from gel slices. Trends Genet. 6:173   DOI   ScienceOn
19 Loria, R., Bukhalid, R. A., Fry, B. A. and King, R. R. 1997. Plant pathogenicity in the genus Streptomyces. Plant Dis. 81 :836-846   DOI   ScienceOn
20 Truper, H. G. and De'Clari, L. 1997. Taxonomic note: necessary correction of specific epithets formed as substantives (nouns) "in apposition". Int. J. Sys. Bacterial. 47:908-909   DOI   ScienceOn
21 Marmur, J. and Doty, P. 1962. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. BioI. 5:109-118   DOI
22 Lee, J. Y. and Hwang, B. K. 2002. Diversity of antifungal actinomycetes in various vegetative sods of Korea Can. J. MicrobioI. 48:407-417
23 Williams, S. T, Goodfellow, M., Alderson, G., Wellington, E. M. H., Sneath, P. H. A. and Sackin, M. J. 1983a. Numerical classification of Streptomyces and related genera. J. Cell. Microbial. 129: 1743-1813
24 Bouchek-Mechiche, K., Pasco, C., Andrivon, D. and Jouan, B. 2000. Differences in host range, pathogenicity to potato cultivars and response to soil temperature among Streptomyces species causing common and netted scab in France. Plant Pathol.49:3-10   DOI   ScienceOn
25 Loria, R., Bukhalid, R .A., Creath, R. A., Leiner, R. H., Oliver, M. and Steffens, J. C. 1995. Differential production ofthaxtomins by pathogenic Streptomyces species in vitro. Phytopathology 85:537-541   DOI   ScienceOn
26 Gussow, H. T. 1914. The systematic ppsition of the organIsm of the common scab. Science 39:431-432   DOI   PUBMED
27 Mandel, M., Igambi, L., Bergendahl, M. L., Dodson, Jr. and Schelgen, E. 1970. Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid. J. Bacteriol. 101:333-338   DOI   PUBMED
28 Pospiech, A. and Neumann, B. 1995. A versatile quick-prep of genomic DNA from Gram-positive bacteria. Trends in Genetics 11:217-218   DOI   ScienceOn
29 Komaki, H., Nemoto, A., Tanaka, Y., Takagi, H., Yazawa, K., Mikami, Y., Shigemori, H., Kobayashi, J. and Ando, A. 1999. Brasilicardin A, a new terpenoid, antibiotic from pathogenic Nocardia brasiliensis: Fermentation, isolation and biologica lactivity. J. Antibiot. 52: 13-19   DOI   ScienceOn
30 Korn-Wendisch, F. and Kutzner, H. J. 1992. The family Streptomycetaceae. In: The Prokaryotes: a Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications. 2nd ed. Balows, A., Traper, H. G., Dworkin, M., Harder, W., and Schleifer, K. H. eds. Springer Verlag, New York. pp. 921-983
31 Thaxter, R. 1891. The potato scab. Conn. Agric. Expt. Sta. Rept. 1890:81-95
32 Cross, T. and Alderson, G. 1988. What weight morphology in current actinomycete taxonomy? In: On Biology of Actinomycetes, Okami, Y., Beppu, T., and Ogawara, H. eds. Biology of actinomycetes 88. Proc. 7th Int. Symp. Tokyo, 1988. Japan Scientific Societies Press, Tokyo. pp. 216-220
33 Waksman, S. A. and Henrici, A. T. 1948. Family II. Actinomycetaceae Buchanam and family Streptomycetaceae Waksman and Henrici. In: Bergeys Manual of Determinative Bacteriology. 6th ed. R. S. Breed, E. G. D. Murray, and A .P. Hitchens, eds. The Williams and Wilks Co., Baltimore. pp. 892-980
34 Momose, I., Kinoshita, N., Sawa, R., Naganowa, H., Iinuma, H., Hamada, M. and Takeuchi, T. 1998. NathramiCin, a new anthracycline antibiotic from Nocardia sp. MJ896-43f17. J. Antibiot.51:130-135   DOI   ScienceOn
35 Mayfield, C. I., Williams, S. T, Ruddick, S. M. and Hatfield, H. L. 1972. Studies on the ecology of actinomyces in soil. IV. Observations on the form and growth of Streptomycetes in soil. Soil BioI. Biochem. 4:79-91   DOI   ScienceOn
36 Stackebrandy, E., Rainey, F. A .and Ward-Rainey, N. L. 1997. Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int. J. Sys. Bacterial. 47:479-491   DOI   ScienceOn
37 Igarashi, M., Kinoshita, N., Ikeda, T., Nakagawa, E., Hamada, M. and Takeuchi, T. 1997. Formarrllcin, anovel antifurigal antibiotic produced by a strain of Sacchdrothrix sp. I. Taxonomy, production, isolation and biological properties. J. Antibiot. 50:926-931   DOI   ScienceOn
38 Neeno-Eckwall, E. C., Kinkel, L. L. and Schottl, J. L. 2001. Competition and antibiosis in the biological control of potato scab. Can. J. Microbial. 47:332-340   DOI   ScienceOn
39 Waksman, S. A. and Henrici, A .T .1943. The nomenclature and classification of the actinomycetes. J. Bacteriol. 46:337-341
40 Kom, F., Weingartner, B. and Kutzner, H. J. 1978. A study of twenty actinophages: Morphology, serological relationship and host range. In: Genetics of the Actinomycetales. E. Fre-erksen, E., Tarnok, I., and Thumin, J. eds. Gustav Fisher Verla&, Stuttgart. pp. 251-270
41 Olsen, G. J. and Woese, C. R. 1993. Ribosomal RNA: a key to phylogeny. FASEB J. 7:113-123   DOI
42 Williams, S. T., Locci, R., Beswick, A., Kurtboke, D. I., Kuznetsov, V. D., Le Monnier, F. J., Long, P. F, Maycroft, K. A., Palma, R. A., Petrolini, B., Quaroni, S., Todd, J. I. and West, M. 1993. Detection and identification of novel actinomycetes. Res. Microbiol. 144:653-656   DOI   ScienceOn
43 Gregory, K. F. and Vaisey, E. B. 1956. Pathogenicity of tyrosinase deficient mutant of Streptomyces scabies. Can. J.' Microbiol. 2:65-71   DOI   ScienceOn
44 Holt, J. G., Krieg, R. R., Sneath, P. H. A., Staley, J. T. and Williams, S. T. 1994. Bergeys Manual of Determinative Bacteriology. Williams & Wilkins, Baltimore, MD. pp. 71-174
45 Shirling, E. B. and Gottlieb, D. 1966. Methods for characterization of Streptomyces species. Int. J. Syst. Bacterial. 16:313-340.   DOI
46 Weisburg, S. T., Goodfellow, M., Wellington, E. M. H., Vickers,J. C., Alderson, G., Sneath, P. H. A, Sackin, M. J. and Mortimer, A. M. 1991. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173:697-703
47 Wilson, C. R., Ransom, L. M. and Pemberton, B. M. 1999. The relative importance of seed-borne inoculum to common scab disease of potato and the efficacy of seed tuber and soil treatments for disease. J. Phytopathol. 147: 13-18
48 Hooker, W. J. and Kent, G. C. 1946. Infection studies with Actinomyces scahies. Phytophathology 36:388-389
49 Johnson, J. L. 1985. Determination of DNA base composition. In: Methods in Microbiology, Vol. 18. Gottschalk, G. ed. Academic Press, London. pp. 1-31   DOI
50 Lambert, D. H. and Loria, R. 1989. Strevtomyces scabies sp. nov., nov., nom. Rev. Int. J. Syst. Bacteriol. 39:387-392   DOI
51 Davis, J. R., McMaster, G. M., Calligan, R. H., Nissley, F. H. and Pavek, J. J. 1976. Influenc, of soil moisture and fungicide treatments on common scab and mineral content of potatoes. Phytophathology 66:228-233   DOI