• Structural Engineering and Mechanics
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• 제53권5호
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• pp.997-1016
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• 2015

The contribution of tensioned concrete between cracks (tension-stiffening) cannot be ignored when analysing deformation of reinforced concrete elements. The tension-stiffening effect is crucial when it comes to adequately estimating the load-deformation response of steel reinforced concrete and the more recently appeared fibre reinforced polymer (FRP) reinforced concrete. This paper presents a unified methodology for numerical modelling of the tension-stiffening effect in steel as well as FRP reinforced flexural members using the concept of equivalent deformation modulus and the smeared crack approach to obtain a modified stress-strain relation of the reinforcement. A closed-form solution for the equivalent secant modulus of deformation of the tensioned reinforcement is proposed for rectangular sections taking the Eurocode 2 curvature prediction technique as the reference. Using equations based on general principles of structural mechanics, the main influencing parameters are obtained. It is found that the ratio between the equivalent stiffness and the initial stiffness basically depends on the product of the modular ratio and reinforcement ratio ($n{\rho}$), the effective-to-total depth ratio (d/h), and the level of loading. The proposed methodology is adequate for numerical modelling of tension-stiffening for different FRP and steel reinforcement, under both service and ultimate conditions. Comparison of the predicted and experimental data obtained by the authors indicates that the proposed methodology is capable to adequately model the tension-stiffening effect in beams reinforced with FRP or steel bars within wide range of loading.

• Molecules and Cells
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• 제21권3호
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• pp.381-388
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• 2006

Heat shock proteins (Hsp) 70 are a ubiquitous family of molecular chaperones involved in many cellular processes. A yeast strain, ssa1/2, with two functionally redundant cytosolic Hsp70s (SSA1 and SSA2) deleted shows thermotolerance comparable to mildly heatshocked wild type yeast, as well as increased protein synthesis and ubiquitin-proteasome protein degradation. Since mRNA abundance does not always correlate well with protein expression levels it is essential to study proteins directly. We used a gel-based approach to identify stress-responsive proteins in the ssa1/2 mutant and identified 43 differentially expressed spots. These were trypsin-digested and analyzed by nano electrospray ionization liquid chromatography tandem mass spectrometry (nESI-LC-MS/MS). A total of 22 non-redundant proteins were identified, 11 of which were confirmed by N-terminal sequencing. Nine proteins, most of which were up-regulated (2-fold or more) in the ssa1/2 mutant, proved to be stress-inducible proteins such as molecular chaperones and anti-oxidant proteins, or proteins related to carbohydrate metabolism. Interestingly, a translational factor Hyp2p up-regulated in the mutant was also found to be highly phosphorylated. These results indicate that the cytosolic Hsp70s, Ssa1p and Ssa2p, regulate an abundance of proteins mainly involved in stress responses and protein synthesis.

• Biomaterials and Biomechanics in Bioengineering
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• 제2권3호
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• pp.127-141
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• 2015

Silver-based systems activated by low intensity direct current continue to be investigated as an alternative antimicrobial for infection prophylaxis and treatment. However there has been limited research on the quantitative characterization of the antimicrobial efficacy of such systems. The objective of this study was to develop a semi-mechanistic pharmacokinetic/pharmacodynamic (PK/PD) model providing the quantitative relationship between the critical system parameters and the degree of antimicrobial efficacy. First, time-kill curves were experimentally established for a strain of Staphylococcus aureus in a nutrientrich fluid environment over 48 hours. Based on these curves, a modified PK/PD model was developed with two components: a growing silver-susceptible bacterial population and a depreciating bactericidal process. The test of goodness-of-fit showed that the model was robust and had good predictability ($R^2>0.7$). The model demonstrated that the current intensity was positively correlated to the initial killing rate and the bactericidal fatigue rate of the system while the anode surface area was negatively correlated to the fatigue rate. The model also allowed the determination of the effective range of these two parameters within which the system has significant antimicrobial efficacy. In conclusion, the modified PK/PD model successfully described bacterial growth and killing kinetics when the bacteria were exposed to the electrically activated silver-titanium implant system. This modeling approach as well as the model itself can also potentially contribute to the development of optimal design strategies for other similar antimicrobial systems.

• 한국지진공학회논문집
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• 제25권3호
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• pp.103-110
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• 2021

A shake table test is conducted for the three-story reinforced concrete building structure using 0.28 g, 0.5 g, 0.75 g, and 1.0 g of seismic input motions based on the Gyeongju earthquake. Computational efforts are made in parallel to explore the mechanical details in the structure. For engineering practice, the elastic modulus of concrete and rebar in the dynamic analysis is reduced to 38% and 50%, respectively, to calibrate the structure's natural frequencies. The engineering approach to the reduced modulus of elasticity is believed to be due to the inability to specify the flexibility of the actual boundary conditions. This aspect may lead to disadvantages of nonlinear dynamic analysis that can distort local stress and strain relationships. The initial elastic modulus can be applied directly without the so-called engineering adjustment with infinite element models with spring and spring-dashpot boundary conditions. This has the advantage of imposing the system flexibility of the structure on the sub-boundary conditions of springs and damping devices to control its sensitivity in a serial arrangement. This can reflect the flexibility of realistic boundary conditions and the effects of system damping (such as the gap between a concrete footing and shake table, loosening of steel anchors, etc.) in scalar quantities. However, these spring and dashpot coefficients can only be coordinated based on experimental results, making it challenging to select the coefficients in-prior to perform an experimental test.

• Structural Engineering and Mechanics
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• 제81권3호
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• pp.349-365
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• 2022

Steel fibre-reinforced concrete (SFRC) is an emerging class of composite for construction. However, a reliable method to assess the flexural behaviour of SFRC structural member is in lack. An analytical technique is proposed for determining the moment-curvature response of concrete beams reinforced with steel fibres and longitudinal bars (R/SFRC members). The behaviour of the tensile zone of such members is highly complex due to the interaction between the residual (tension softening) stresses of SFRC and the tension stiffening stresses. The current study suggests a transparent and mechanically sound method to combine these two stress concepts. Tension stiffening is modelled by the reinforcement-related approach assuming that the corresponding stresses act in the area of tensile reinforcement. The effect is quantified based on the analogy between the R/SFRC member and the equivalent RC member having identical geometry and materials except fibres. It is assumed that the resultant tension stiffening force for the R/SFRC member can be calculated as for the equivalent RC member providing that the reinforcement strain in the cracked section of these members is the same. The resultant tension stiffening force can be defined from the moment-curvature relation of the equivalent RC member using an inverse technique. The residual stress is calculated using an existing model that eliminates the need for dedicated mechanical testing. The proposed analytical technique was validated against test data of R/SFRC beams and slabs.

• Structural Engineering and Mechanics
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• 제84권4호
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• pp.437-452
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• 2022

For the first time, the higher-order shear and normal deformable plate theory (HOSNDPT) is used for the vibration and flutter analyses of the multilayer functionally graded graphene platelets reinforced composite (FG-GPLRC) plates under supersonic airflow. For modeling the supersonic airflow, the linear piston theory is adopted. In HOSNDPT, Legendre polynomials are used to approximate the components of the displacement field in the thickness direction. So, all stress and strain components are encountered. Either uniform or three kinds of non-uniform distribution of graphene platelets (GPLs) into polymer matrix are considered. The Young modulus of the FG-GPLRC plate is estimated by the modified Halpin-Tsai model, while the Poisson ratio and mass density are determined by the rule of mixtures. The Hamilton's principle is used to obtain the governing equations of motion and the associated boundary conditions of the plate. For solving the plate's equations of motion, the Galerkin approach is applied. A comparison for the natural frequencies obtained based on the present investigation and those of three-dimensional elasticity theory shows a very good agreement. The flutter boundaries for FG-GPLRC plates based on HOSNDPT are described and the effects of GPL distribution patterns, the geometrical parameters and the weight fraction of GPLs on the flutter frequencies and flutter aerodynamic pressure of the plate are studied in detail. The obtained results show that by increasing 0.5% of GPLs into polymer matrix, the flutter aerodynamic pressure increases approximately 117%, 145%, 166% and 196% for FG-O, FG-A, UD and FG-X distribution patterns, respectively.

• Wind and Structures
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• 제37권4호
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• pp.289-302
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• 2023

Insulating glass units (IGUs) have been widely used in buildings in recent years due to their superior thermal insulation performance. However, because of the panel reciprocating motion and fatigue deterioration of sealants under long-term wind loads, many IGUs have the problem of early failure of watertight properties in real usage. This study aimed to propose a statistical method for wind-induced deflection of IGU panels during the whole life service period, for further precise analysis of the accumulated fatigue damage at the sealed part of the edge bond. By the estimation of the wind occurrence regularity based on wind pressure return period, the events of each wind speed interval during the whole life were obtained for the IGUs at 50m height in Beijing, which are in good agreement with the measured data. Also, the wind-induced deflection analysis method of IGUs based on the formula of airspace coefficient was proposed and verified as an improvement of the original stiffness distribution method with the average relative error compared to the test being about 3% or less. Combining the two methods above, the deformation of the outer and inner panes under wind loads during 30 years was precisely calculated, and the deflection and stress state at selected locations were obtained finally. The results show that the compression displacement at the secondary sealant under the maximum wind pressure is close to 0.3mm (strain 2.5%), and the IGUs are in tens of thousands of times the low amplitude tensile-compression cycle and several times to dozens of times the relatively high amplitude tensile-compression cycle environment. The approach proposed in this paper provides a basis for subsequent studies on the durability of IGUs and the wind-resistant behaviors of curtain wall structures.

• 한국축산식품학회지
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• 제44권1호
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• pp.39-50
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• 2024

The projected growth of global meat production over the next decade is attributed to rising income levels and population expansion. One potentially more pragmatic approach to mitigating the adverse externalities associated with meat production involves implementing alterations to the production process, such as transitioning to cultured meat, hybrid cultured meat, and meat alternatives. Cultured meat (CM) is derived from animal stem cells and undergoes a growth and division process that closely resembles the natural in vivo cellular development. CM is emerging as a widely embraced substitute for traditional protein sources, with the potential to alleviate the future strain on animalderived meat production. To date, the primary emphasis of cultured meat research and production has predominantly been around the ecological advantages and ethical considerations pertaining to animal welfare. However, there exists substantial study potential in exploring consumer preferences with respect to the texture, color, cuts, and sustainable methodologies associated with cultured meat. The potential augmentation of cultured meat's acceptance could be facilitated through the advancement of a wider range of cuts to mimic real muscle fibers. This review examines the prospective commercial trends of hybrid cultured meat. Subsequently, the present state of research pertaining to the advancement of scaffolding, coloration, and muscle fiber development in hybrid cultured meat, encompassing plant-based alternatives designed to emulate authentic meat, has been deliberated. However, this discussion highlights the obstacles that have arisen in current procedures and proposes future research directions for the development of sustainable cultured meat and meat alternatives, such as plant-based meat production.

• International Journal of Computer Science & Network Security
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• 제24권1호
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• pp.226-234
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• 2024

The frequent handover problem and playing ping-pong effects in 5G (5th Generation) ultra-dense networking cannot be effectively resolved by the conventional handover decision methods, which rely on the handover thresholds and measurement reports. For instance, millimetre-wave LANs, broadband remote association techniques, and 5G/6G organizations are instances of group of people yet to come frameworks that request greater security, lower idleness, and dependable principles and correspondence limit. One of the critical parts of 5G and 6G innovation is believed to be successful blockage the board. With further developed help quality, it empowers administrator to run many systems administration recreations on a solitary association. To guarantee load adjusting, forestall network cut disappointment, and give substitute cuts in case of blockage or cut frustration, a modern pursuing choices framework to deal with showing up network information is require. Our goal is to balance the strain on BSs while optimizing the value of the information that is transferred from satellites to BSs. Nevertheless, due to their irregular flight characteristic, some satellites frequently cannot establish a connection with Base Stations (BSs), which further complicates the joint satellite-BS connection and channel allocation. SF redistribution techniques based on Deep Reinforcement Learning (DRL) have been devised, taking into account the randomness of the data received by the terminal. In order to predict the best capacity improvements in the wireless instruments of 5G and 6G IoT networks, a hybrid algorithm for deep learning is being used in this study. To control the level of congestion within a 5G/6G network, the suggested approach is put into effect to a training set. With 0.933 accuracy and 0.067 miss rate, the suggested method produced encouraging results.

• Advances in nano research
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• 제17권2호
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• pp.181-195
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• 2024

This study introduces a novel functionally graded material model, termed the "Coated Functionally Graded Graphene-Reinforced Composite (FG GRC)" model, for investigating the free vibration response of plates, highlighting its potential to advance the understanding and application of material property variations in structural engineering. Two types of coated FG GRC plates are examined: Hardcore and Softcore, and five distribution patterns are proposed, namely FG-A, FG-B, FG-C, FG-D, and FG-E. A modified displacement field is proposed based on the higher-order shear deformation theory, effectively reducing the number of variables from five to four while accurately accounting for shear deformation effects. To solve the equations of motion, an analytical solution based on the Galerkin approach was developed for FG GRC plates resting on a viscoelastic Winkler/Pasternak foundation, applicable to various boundary conditions. A comprehensive parametric analysis elucidates the impact of multiple factors on the fundamental frequencies. These factors encompass the types and distribution patterns of the coated FG GRC plates, gradient material distribution, porosities, nonlocal length scale parameter, gradient material scale parameter, nanoplate geometry, and variations in the elastic foundation. Our theoretical research aims to overcome the inherent challenges in modeling structures, providing a robust alternative to experimental analyses of the mechanical behavior of complex structures.