• Korean Journal of Environmental Biology
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• v.33 no.2
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• pp.248-255
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• 2015

Induction of regeneration and maturation of the free-living gametophytes of Saccharina japonica was studied at four temperatures (5, 10, 15 and $20^{\circ}C$), four levels of irradiance (5, 10, 20 and $40{\mu}mol\;m^{-2}\;s^{-1}$) and three photoperiods (14 : 10, 12 : 12 and 10 : 14 h L : D). Female gametophyte fragments were maintained in active regeneration without reaching sexual maturity under conditions of $15^{\circ}C$, $20{\mu}mol\;m^{-2}\;s^{-1}$, 10 : 14 h (L : D), whereas the conditions for male gametophytes were slightly different at $15^{\circ}C$, $5{\mu}mol\;m^{-2}\;s^{-1}$, 14 : 10 h (L : D). The sexual maturation of female and male gametophytes took place under $5^{\circ}C$, $20{\sim}40{\mu}mol\;m^{-2}\;s^{-1}$, 10 : 14 h (L : D) and $15^{\circ}C$, $40{\mu}mol\;m^{-2}\;s^{-1}$, 10 : 14 h (L : D), respectively. These results provide basic information for controlling the regenerationand maturation of free-living gametophytes for strain improvement and cross breeding of S. japonica.

• Microbiology and Biotechnology Letters
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• v.26 no.6
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• pp.490-496
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• 1998

Aspergillus oryzae producing high proteolytic enzyme was isolated from soybean koji and named tentatively A. oryzae O-1. A. oryzae U-1 was obtained by mutation of A. oryzae O-1 with ultraviolet (UV)-irradiation and produced 14 times higher pretense activity compared with A. oryzae O-1. A. oryzae E-1 was acquired by treatment of A. oryzae U-1 with 0.5 M ethylmethanesulfonate (EMS) for 6 min at 3$0^{\circ}C$ and produced 39 times higher proteolytic activity than A. oryzae O-1. With protoplast fusion between A. oryzae O-1 and A. oryzee E-1 in the presence of polyethylenegylcol (PEG)-CaCl$_2$, proteolytic activity was increased to 82 times compared to A. oryzae O-1, and the fusant was named A. oryzae PF. The activities of the cultures containing proteolytic enzymes produced by the strains were determined to be 0.23 U/$m\ell$ for A. oryzae O-1, 3.29 U/$m\ell$ for A. oryzae U-1, 8.91 U/$m\ell$ for A. oryzae E-1, and 19.0 U/$m\ell$ for A. oryzae PF.

• Microbiology and Biotechnology Letters
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• v.26 no.2
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• pp.151-160
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• 1998

The Leuconostoc paramesenteroides dominated at refrigeration temperature range was isolated from kimchi, and improved its growth properties by mutation for competitive growth against Lactobacillus plantarum at lower pH. It was found that the minimal pH for the wild type Leuconostoc paramesenteroides Pw growth was pH 4.5 adjusted with HCI and pH 5.0 adjusted with organic-mixture (lactic acid:acetic acid=1:2), respectively. The mutant P-100 could grow in pH 4.0, 4.5, respectively, in MRS broth. Two strains Pw and P-100 were added into kimchi as starter and compared the quality characteristics of kimchi. The total acceptability of Pw and P-100 inoculated kimchi were evaluated better than that of control kimchi (no starter added) by sensory test and extended the optimal pH range of kimchi up to about 2.2, 2.5 times, respectively. In kimchi added P-100, the succinic acid was more abundant than others and the total number of Lactobacillus plantarum was down about 2.5 times in contrast to control kimchi.

• Journal of Korean Neurosurgical Society
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• v.46 no.6
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• pp.532-537
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• 2009

Objective : Recently, motion preservation has come to the forefront of emerging technologies in spine surgery. This is the important background information of the emergence of cervical arthroplasty as an alternative to arthrodesis that offers the promise of restoring normal spinal movement and reduces a kinematic strain on adjacent segments. The study was designed to evaluate early surgical outcome and radiological effects of $Bryan^{(R)}$ cervical disc prosthesis. Methods : The authors retrospectively reviewed radiographic and clinical outcomes in 52 patients who received the $Bryan^{(R)}$ Cervical Disc prosthesis, for whom follow-up data were available. Static and dynamic radiographs were measured by computer to determine the angles formed by the endplates of the natural disc preoperatively, those formed by the shells of the implanted prosthesis, the angle of functional spine unit (FSU), and the C2-7 Cobb angle. The range of motion (ROM) was also determined radiographically, whereas clinical outcomes were assessed using Odom's criteria, visual analogue pain scale (VAS) and neck disability index (NDI). Results : A total of 71 $Bryan^{(R)}$ disc were placed in 52 patients. A single-level procedure was performed in 36 patients, a two-level procedure in 13 patients, and a three-level procedure in 3. Radiographic and clinical assessments were made preoperatively. Mean follow-up duration was 29.2 months, ranging from 6 to 36 months. All of the patients were satisfied with the surgical results by Odom's criteria, and showed significant improvement by VAS and NDI score (p < 0.05). The postoperative ROM of the implanted level was preserved without significant difference from preoperative ROM of the operated level (p < 0.05). 97% of patients with a preoperative lordotic sagittal orientation of the FSU were able to maintain lordosis. The overall sagittal alignment of the cervical spine was preserved in 88.5% of cases at the final follow up. Interestingly, preoperatively kyphotic FSU resulted in lordotic FSU in 70% of patients during the late follow up, and preoperatively kyphotic overall cervical alignment resulted in lordosis in 66.6% of the patients postoperatively. Conclusion : Arthroplasty using the $Bryan^{(R)}$ disc seemed to be safe and provided encouraging clinical and radiologic outcome in our study. Although the early results are promising, this is a relatively new approach, therefore long-term follow up studies are required to prove its efficacy and its ability to prevent adjacent segment disease.

• Journal of Biosystems Engineering
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• v.7 no.2
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• pp.57-71
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• 1983

마찰식 정미기의 성능에 영향을 미치는 기계설계 및 작동상의 인자로는 스크린의 형태, 롤러의 형태, 롤러회전속도, 출구 저항 등 여러가지가 있다. 이러한 요인들에 대한 연구가 본 논문의 저자에 의해서 시리즈로 연구되어 왔으며, 본 연구는 그의 일환으로 스크린의 단면형상(6각, 8각, 12각 그리고 원통형), 스크린에 뚫려있는 슬랏의 기울기($15^{\circ}$, $30^{\circ}$, $45^{\circ}$ 그리고 $-45^{\circ}$), 그리고 출구 저항이 정백미의 질과 양, 정백효율 그리고 정백실내의 정백압력 등에 미치는 영향을 규명하기 위해 수행되었다. 이 실험에 사용된 공시 정미기는 임도정 공장에서 널리 사용되고 있는 흡입마찰식이었며, 사용된 시료는 밀양 23호인 통일계품종이었다. 각 처리마다 동력 소모량과 정백실내의 정백압력을 측정하기 위해서 "KYOWA" 스트레인 게이지 시스템(strain gauge system), 토오크 변환기, 압력변환기 등이 사용되었다. 본 실험 결과를 요약하면 다음과 같다. 1) 정백과정중에 정백실내의 정백압력은 입구에서 가장 높았고 출구쪽으로 갈수록 점차 감소하였다. 평균 정백압력도 순환 횟수에 따라서 변화 하였는데 첫번째 순환에서 가장 높았고 순환횟수가 증가함에 따라 감소하였다. 2) 본 실험에서 사용한 출구저항 수준은 출구저항 수준 1에서 요구되는 정백도의 백미를 얻기 위한 순환 횟수는 2회 이었고 출구저항 수준 2에서는 4회 이었는데, 이를 출구저항 수준이 정백압력, 정백율, 완전미수율 및 도정 효율에 가장 큰 영향을 주었다. 3) 스크린에 뚫려있는 슬랏 각도가 $15^{\circ}$에서 $45^{\circ}$로 증가함에 따라서 정백실내의 평균 정백압력은 감소하였고, 완전미수율과 도정 효율은 증가하였으나, 일반적으로 도정수율은 감소하는 경향을 보였다. $-45^{\circ}$의 슬랏 각도를 가진 스크린에서는 슬랏 각도 $15^{\circ}$인 스크린에서와 거의 비슷한 정백압력 수준을 나타냈으나 완전미와 정백미의 생산은 매우 낮았다. 4) 스크린 표면에 기다란 강편 (보통 "띠"라고 부름)을 붙인 원통과 12각형의 스크린에서는 강편을 붙이지 않은 6각형과 8각형 스크린에서 보다는 비교적 높은 정백압력을 나타냈다. 전자는 후자에 비해서 정백효율은 높았으나 완전미수율은 떨어졌다. 5) 정백실내에서 가장 낮은 정백압력을 나타낸 8각형 스크린은 높은 도정수율과 완전미수율을 가져왔으나 상대적으로 정백효율은 낮게 나타났다. 6) 정백실내의 반경 방향의 평균 정백압력($P_R$)과 완전미수율($Y_h$)은 다음과 같은 1차적인 역비례 관계가 있었으며(평균 정백압력 범위는 0.5-0.9kg/$cm^2$), $Y_h=-28.661P_R+84.860$ ($r^2=0.858$) 정백효율($Y_e$)과 정백압력($P_R$) 사이에는 다음과 같은 2차적인 관계가 존재함을 알 수 있었다. $Y_e=-597.5P_{R^2}+929.96P_R-210.15$ ($r^2=0.759$) 정백효율은 정백압력이 0.7-0.8kg/$cm^2$일 때 가장 높았으며, 이때 변이도 가장 심한 것으로 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.6
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• pp.976-982
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• 2007

This study investigated whether supplemental Cu, Fe, Zn, and Mn are needed in a practical diet for pullets. Four hundred and twenty females of an egg-laying strain (1-d-old, Lohmann Brown Layer) were randomly distributed into 4 groups, consisting of 7 replicates of 15 birds each. During the 18-week experimental period, chicks were given three basal diets in sequence, each with single or multiple Mn, Zn and Cu supplementation to improve the mineral balance gradually. In the Control, no Mn, Zn, and Cu were added; in the single Mn supplemented group (sMn) Mn was added to 120, 60, and 60 mg/kg for 1-6, 7-12, and 13-18 weeks of age, respectively; in the multiple Mn and Zn supplemented group (mMnZn), Mn was added to 180, 90, and 90 mg/kg and Zn was added to 120, 105, and 105 mg/kg for 1-6, 7-12, and 13-18 weeks of age, respectively; in the multiple Mn, Zn, Cu supplemented group (mMnZnCu), Mn, Zn, and Cu were added to the same multiple of basal Fe concentration relative to NRC (1994) recommendations. Energy and protein metabolizability were determined by subtracting energy/protein intake by energy/protein excretion (from both feces and urine) and dividing by energy/protein intake. There were no statistically significant differences between groups in terms of feed intake, final body weight or tibia length throughout the experiment. Optimal growth performance was observed in the Control, while adding trace minerals to basal diets tended to result in decreased productive performance. Protein metabolizability was increased by mMnZn and mMnZnCu treatments, but energy metabolizability was not affected. Concentrations of Mn, Zn, Cu in excreta varied greatly related to dietary content, and the retentions of Cu, Fe, Zn and Mn were all increased due to the improvement of mineral balance. Based on these results, it is suggested that the concentrations of Cu, Fe, Zn and Mn in typical basal diets used in this study were adequate for normal growth for pullets from 1 to 18 weeks of age.

• Journal of Microbiology and Biotechnology
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• v.9 no.1
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• pp.22-31
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• 1999

Saccharomyces fermentati and Leuconostoc mesenteroides were isolated from a traditional kimchi, and then the Leu. mesenteroides was mutated to the acid-tolerant mutant Leu. mesenteroides M-l00. In the result of growth properties in MRS broth with various pHs adjusted with HCl and acid solution (latic acid:acetic acid=1:2), an acid-tolerant mutant Leu. mesenteroides M-100 showed more increased ability for growth than its wild strain at various temperatures. The strains were used as starters for the fermentation of kimchi. The experiments were performed with classified experimental groups (Group I, control kimchi; Group II, addition of YK-19 only; Group III, addition of M-100 only; Group IV, addition of mixture of M-100 and YK-19), and their pH, total acidity, reducing sugars content, organic acid productivity, organoleptic tests, and microfloral changes were compared. As a result, in pH and acidity, the optimal ripening period of Group IV was about 13.5 days (i.e. from the 8.5 to 22 days of fermentation). This result indicates that the optimal ripening period of Group IV was about 1.5 times longer than that of Group I. Furthermore, Group IV was edible to 28 days of fermentation. In addition, high contents of succinc acid was observed in Group IV. Group IV was also highly ranked on the organoleptic test. During the fermentation of kimchi, the number of Leuconostoc sp. in group I reduced after 7 days; however, the number of Leuconostoc sp. in Group II, III, and IV decresed after 14 days of fermentation. An especially high number of Leu. sp. was observed in Group IV, and this gave better flavor of kimchi than any other during the whole fermentation period. Citric acid, tartaric acid, succinic acid, fumaric acid, and lactic acid were detected in the kimchi, and a significant increase in the concentration of lactic acid during fermentation was observed in the all experimental groups.

• Journal of the Korean Society for Railway
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• v.10 no.6
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• pp.701-708
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• 2007

A tilting train, which was developed to run the curve section without reducing the speed and compromising the riding quality, can improve the speed so as to reduce the travel time, compared to the existing trains. Then the force generated by the train operation to the track is in proportion to train operation speed, which means the track shall bear the increased force as much as the increase in train operation speed. Particularly, wheel load and lateral wheel load generated by train operation and distributed to the rail tend to cause the track to suffer the strain and furthermore the severe disaster such as derailment. To deal with such problem and ensure the train will run safety and stably, the tolerance in wheel load change, lateral wheel load and derailment coefficient was determined for quantitative evaluation of the train operation stability. In this study, derailment coefficient of inner and outer rail at existing curve section of tilting train was determined to evaluate the curve radius, possibility of acceleration and the need of rail improvement, which was then compared with the existing traditional train and high speed train. Conducting the quantitative evaluation of dynamic wheel load and lateral wheel load of each train, which was based on field survey, derailment coefficient and static & dynamic wheel load change, which serve the evaluation criteria of train operation stability, were determined for comparison with the standards, thereby analyzing the stability of the tilting train.

• Korean Journal of Medicinal Crop Science
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• v.15 no.5
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• pp.339-345
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• 2007

Field performance and morphological characterization was conducted on seven transgenic lines of Codonopsis lanceolata expressing ${\gamma}-TMT$ gene. The shoots were obtained from leaf explants after co-cultivation with Agrobacterium tume-faciens strain LBA 4404 harboring a binary vector pYBI 121 that carried genes encoding ${\gamma}-Tocopherol$ methyltransferase gene (${\gamma}-TMT$) and a neomycin phosphotransferase II gene (npt II) for kanamycin resistance. The transgenic plants were transferred to a green house for acclimation. Integration of T-DNA into the $T_0\;and\;T_1$ generation of transgenic Codonopsis lanceolata genome was confirmed by the polymerase chain reaction and southern blot analysis. The progenies of transgenic plants showed phenotypic differences within the different lines and with relative to control plants. When grown in field, the transgenic plants in general exhibited increased fertility, significant improvement in the shoot weight, root weight, shoot height and rachis length with relation to the control plants. However, all seven independently derived transgenic lines produced normal flower with respect to its shape, size, color and seeds number at its maturity. Indicating that the addition of a selectable marker gene in the plant genome does not effect on seed germination and agronomic performance of transgenic Codonopsis lanceolata. $T_1$ progenies of these plants were obtained and evaluated together with control plant in a field experiment. Overall, the agronomic performance of $T_1$ progenies of transgenic Codonopsis lanceolata showed superior to that of the seed derived non-transgenic plant. In this study, we report on the morphological variation and agronomic performance of transgenic Codonopsis lanceolata developed by Agrobacterium transformation.

• Korean Journal of Social Welfare
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• v.60 no.4
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• pp.31-53
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• 2008

This study intends to verify the poverty characteristics of the rural elderly households so as to provide more specific data for the intervention of social welfare in the improvement of quality of life of the rural elderly. According to my study of the absolute poverty ratio, the poverty gap, the relative poverty ratio, the subjective poverty ratio, the subjective economic level and the life satisfaction level of the rural elderly households in comparison with those of the other groups, the conclusion is as follows: First, the total living cost of the rural elderly households is rather low with the food cost taking about 30% of the total living cost. Second, in terms of the poverty ratio based on socio-demographic characteristics, higher poverty ratio show among women, older people and households of fewer members. Third, 82.2% of the rural elderly households living with an income below the minimum living cost is found around the poverty line. Fourth, the rural elderly households show a comparatively higher poverty ratio than the other groups in the absolute and relative poverty, but lower economic strain than other groups in the subjective poverty ratio and life satisfaction level. In short, the rural elderly households' poverty level is generally high and therefore need ways of active economic supports, while a political approach to the subjective needs of the regional and intergenerational groups is also required.