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http://dx.doi.org/10.11626/KJEB.2015.33.2.248

Isolation, Regeneration and Maturation Induction of Free-living Gametophytes of Saccharina japonica (Phaeophyceae)  

Hwang, Eun Kyoung (Seaweed Research Center, NFRDI)
Yoo, Ho Chang (Seaweed Research Center, NFRDI)
Kim, Se Mi (Seaweed Research Center, NFRDI)
Baek, Jae Min (Seaweed Research Center, NFRDI)
Park, Chan Sun (Department Marine Resources, Mokpo National University)
Publication Information
Korean Journal of Environmental Biology / v.33, no.2, 2015 , pp. 248-255 More about this Journal
Abstract
Induction of regeneration and maturation of the free-living gametophytes of Saccharina japonica was studied at four temperatures (5, 10, 15 and $20^{\circ}C$), four levels of irradiance (5, 10, 20 and $40{\mu}mol\;m^{-2}\;s^{-1}$) and three photoperiods (14 : 10, 12 : 12 and 10 : 14 h L : D). Female gametophyte fragments were maintained in active regeneration without reaching sexual maturity under conditions of $15^{\circ}C$, $20{\mu}mol\;m^{-2}\;s^{-1}$, 10 : 14 h (L : D), whereas the conditions for male gametophytes were slightly different at $15^{\circ}C$, $5{\mu}mol\;m^{-2}\;s^{-1}$, 14 : 10 h (L : D). The sexual maturation of female and male gametophytes took place under $5^{\circ}C$, $20{\sim}40{\mu}mol\;m^{-2}\;s^{-1}$, 10 : 14 h (L : D) and $15^{\circ}C$, $40{\mu}mol\;m^{-2}\;s^{-1}$, 10 : 14 h (L : D), respectively. These results provide basic information for controlling the regenerationand maturation of free-living gametophytes for strain improvement and cross breeding of S. japonica.
Keywords
free-living gametophyte; maturation; regeneration; Saccharina japonica;
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