• Korean Journal of Microbiology
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• v.25 no.3
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• pp.221-228
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• 1987

The extracellular adenine deaminase from Nocardioides sp. J-275L was purified by the following techniques: ammonium sulfate fractionation, DEAE-Cellulose, DEAE-Sephadex A-50 column chromatography, and Sephacryl S-200 superfine gel filtration. The enzyme was partially purified about 3889.5-fold with about 5.2% yield by these procedures. The molecular weight of the enzyme was 39,000 by a calibrated Sephacryl S-200 superfine column chromatography. The enzyme was stable at pH 7.5 and up to $40^{\circ}C$. Glycerol was effective on the stabilization of the enzyme during storage. The optimum pH and temperature of the enzyme were around pH 7.5 and $40^{\circ}C$, respectively. The apparent Michaelis constant Km of the enzyme for adenine was $7.4\times 10^{-5}$M. The purine analogues, 6-chloropurine, 2,6-diaminopurine, 6-bromopurine, 4-aminopyrazolo [3.4-d]pyrimidine, and 8-azaadenine were substrates for the enzyme. 6-Dimethylaminopurine was a competitive inhibitor of the enzyme. The enzyme was inhibited by 1mM of $Cu^{2+}, Fe^{3+}, Pb^{2+}, Hg^{2+}$, and $Ag^{+}$, and 1mM of $\alpha$,$\alpha$'-dipyridyl, pentachlorophenol, and pCMB.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.3
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• pp.411-416
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• 2002

Yogurt base was prepared from whole milk and skim milk added with 0.2∼1.0% (w/v) of Saururus chinensis(Lour.) Bail water extract (SCe) and fermented with lactic acid bacterias (the mixed strain of Streptococcas themophilus and Lactobacillus bulgaricus) at 37$\^{C}$ for 24 hr. Quality characteristics of the yogurt were evaluated in terms of acid production, number of viable cells, viscosity and sensory property during lactic acid fermentation. The composition of organic acids was also measured by HPLC. Addition of SCe stimulated the growth of lactic acid bacteria and remarkably enhanced the acid production. The viscosity and lactic acid content of yogurt were also increased by addition of SCe. The sensory score of yogurt added with 0.4% of SCe was significantly higher than other groups in taste and overall acceptability The storage abilities of yogurts added with SCe were relatively good at 5$\^{C}$ for 15 days.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.3
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• pp.433-437
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• 2002

Human milk is normally contaminated with various microorganisms, which seem to produce no ill effects. A contamination of hand-expressed and pump-expressed human milk is a major concern in the collection of storage milk. In this study we compared milks collected by two methods, hand expression and suction breast pump, to quantify differences in the degree of bacterial contamination. Thirty-one samples had been manually expressed. The mean of total bacterial counts was 10,600 CFU/mL (range: 360 ∼59,200 CFU/mL) and coliform counts was 43 CFU/mL (range: 20 ∼ 1,060 CFU/mL) in these samples. Whereas in the 118 breast pump-expressed samples, the mean of total bacterial counts was 20,200 CFU/mL (range: 240 ∼ 492,000 CFU/mL) and coliform counts was 158 CFU/mL (range: 4∼10,600 CFU/mL). There was no bacterial growth when the samples were incubated for 10 days at 4$\^{C}$. We also compared total bacterial growth in colostrum and in matured human milk for 24 hr at 20$\^{C}$ and 30$\^{C}$. Although bacterial growth had not shown for 24 hr at 20$\^{C}$, but shown slight growth in colostrum and rapidly increase in matured human milk for 24 hr at 30$\^{C}$. The coliform bacteria in all samples, particulary in formula milk, had grown at 20$\^{C}$ and 30$\^{C}$.

• Journal of KIISE:Databases
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• v.28 no.3
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• pp.384-394
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• 2001

In this paper, we propose AST-AET(Average valid Start Time-Average valid End Time) data migration strategy based on the storage structure where temporal data is divided into a past segment, a current segment, and a future segment. We define AST and AET which are used in AST-AET data migration strategy and also define entity versions to be stored in each segment. We describe methods to compute AST and AET, and processes to search entity versions for migration and move them. We compare average response times for user queries between AST-AET data migration strategy and the existing LST-GET(Least valid Start Time-Greatest valid End Time) data migration strategy. The experimental results show that, when there are no LLTs(Long Lived Tuples), there is little difference in performance between the two migration strategies because the size of a current segment is nearly equal. However, when there are LLTs, the average response time of AST-AET data migration strategy is smaller than that of LST-GET data migration strategy because the size of a current segment of LST-GET data migration strategy becomes larger. In addition, when we change average interarrival times of temporal queries, generally the average response time of AST-AET data migration strategy is smaller than that of LST-GET data migration strategy.

• Restorative Dentistry and Endodontics
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• v.35 no.2
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• pp.106-115
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• 2010

The purpose of this study was to evaluate the microtensile bond strength (${\mu}TBS$), failure modes and bonding interfaces of self-etching and three self-adhesive resin cements to dentin and indirect composite resin. Cylindrical composite blocks (Tescera, Bisco Inc.) were luted with resin cements (PA: Panavia F 2.0, Kuraray Medical Inc., RE: RelyX Unicem Clicker, 3M ESPE., MA: Maxem, Kerr Co., BI: BisCem, Bisco Inc.) on the prepared occlusal dentin surfaces of 20 extracted molars. After storage in distilled water for 24 h, $1.0\;mm\;{\times}\;1.0\;mm$ composite-dentin beams were prepared. ${\mu}TBS$ was tested at a cross-head speed of 0.5 mm/min. Data were analyzed with one-way ANOVA and Tukey's HSD test. Dentin sides of all fractured specimens and interfaces of resin cements-dentin or resin cements-composite were examined at FESEM (Field Emission-Scanning Electron Microscope). In conclusion, PA and RE showed higher bond strength and closer adaptation than MA and BI when indirect composite blocks were luted to dentin using a self-etching and three self-adhesive resin cements.

• The KIPS Transactions:PartA
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• v.9A no.4
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• pp.399-404
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• 2002

Along the evolution of information and communication technologies, manufacturing embedded systems such as PDA (personal digital assistant), HPC (hand -held PC), settop box. and information appliance became realistic. And RTOS (real-time operating system) and filesystem have been played essential re]os within the embedded systems as well. For the filesystem of embedded systems, flash memory has been used extensively instead of traditional hard disk drives because of embedded system's requirements like portability, fast access time, and low power consumption. Other than these requirements, nonvolatile storage characteristic of flash memory is another reason for wide adoption in industry. However, there are some technical challenges to cope with to use the flash memory as an indispensable component of the embedded systems. These would be relatively slow cleaning time and the limited number of times to write-and-clean. In this paper, a new cleaning policy is proposed to overcome the problems mentioned above and relevant performance comparison results will be provided. Ranking cleaning policy(RCP) decides when and where to clean within the flash memory considering the cost of cleaning and the number of times of cleaning. This method will maximize not only the lifetime of flash memory but also the performance of access time and manageability. As a result of performance comparison, RCP has showed about 10 ~ 50% of performance evolution compared to traditional policies, Greedy and Cost-benefit methods, by write throughputs.

• Korean Journal of Food Science and Technology
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• v.48 no.5
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• pp.445-453
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• 2016

Eighty-four strains of lactic acid bacteria were isolated from Korean fermented foods for the production of fermented apple juice. Among these strains, the JBE245 strain that showed rapid growth and food functionality was selected and identified as Lactobacillus plantarum. This strain reached the stationary phase after 24 h fermentation at $30^{\circ}C$ with $1.5{\times}10^8$ colony forming unit (CFU)/mL of viable cells, and maintained its viability levels even after 14 days of storage. During fermentation, the ${\alpha}-glucosidase$ inhibitory activity (40.4%), total polyphenol content (583.6 mg gallic acid equivalent (GAE)/mL), and 2,2-diphenyl-l-picryl-hydrazyl hydrate (DPPH) radical scavenging activity (52%) were increased. As judged by a sensory test, the overall preference for the fermented juice (4.22) was comparable to that for the unfermented juice (4.72), indicating that fermentation does not significantly affect the sensory characteristics of apple juice. Consequently, the fermented beverage containing L. plantarum JBE245 and apple juice is a promising functional health food.

• FLOWER RESEARCH JOURNAL
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• v.17 no.1
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• pp.40-43
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• 2009

'Glory Pink' an interspecific lily cultivar was released in 2007 at National Horticultural Research Institute (NHRI), Rural Development Administration (RDA), Suwon, Korea. The cross and ovule culture was made between FA interspecific hybrid 'FA97-30' (L. formolongi 'Raizan' ${\times}$ Asiatic lily 'A61'), a red colored cultivar, and Asiatic lily 'Sanzio', light red colored cultivar in 2001. Multiplication and bulbing, and characteristic tests were conducted from 2005 to 2006. Flowering time is around the first of July and the plant height is 131.7 cm. Flowers are upward-facing and red-pink (RHS 63C), with size of 11.3 cm. Petal length and width is 9.1 cm and 4.1 cm, respectively. Leaves are 13.8 cm long, 1.4 cm wide. The throat is dark green, and the stigma is light yellow and pollen is light brown. The weight and size of bulb is 51.1 g and 13.2 cm, respectively. For long-term storage, bulbs can be stored under -1 to -2 for year-round forcing.

• Journal of Korean Society on Water Environment
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• v.37 no.2
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• pp.75-91
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• 2021

Ocean dumping manure was prohibited in Korea until the end of 2012. Undumped manure was treated by resourcification, purification, and public process in a facility. But poor storage of untreated manure and overproduced compost can pollute river water. In this study, linear regression, Mann-Kendall, Seasonal Mann-Kendall, LOWESS trend, and Spearman's correlation analysis were conducted for investigating the changes in the water quality of Bukhan and Imjin river before and after the prohibition of ocean dumping. In 2017, the Imjin Riv er basin had more than 5.7 times liv estock breeding heads than the Bukhan Riv er basin and more than 3.5 times livestock manure. The areas of land, farmland, and nutrient balance were also higher in the Imjin River basin. Since the two basins have different environments, it is expected that the characteristics of changes in water quality due to the increase in livestock manure generation and treatment methods will be different when comparing the water quality trends before and after the ban. The result of the trend analysis revealed a decrease in EC before the ban but an increase afterward. T-N and BOD of Imjin river increased slightly before the ban but demonstrated great enhancement thereafter. Other sites and T-P exhibited no trend. The correlation analysis result showed that the increase in the number of livestock rearing was correlated with the increase in T-P of Bukhan River and BOD of Imjin River. The increase in livestock manure production was not correlated with the Imjin River basin, while a correlation was observed with the Bukhan River with an increase in T-N and EC and the decrease in T-P.

• Korean Journal of Food Science and Technology
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• v.50 no.6
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• pp.621-628
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• 2018

We developed a quantitative microbial risk assessment model for determning the effect of seasoning on Clostridium perfringens behavior in beef jerky under aerobic and anaerobic conditions. C. perfringens was not detected (<0.5 log CFU/g) in beef jerky samples (n=275), regardless of storage conditions or the presence of seasoning. Survival models of C. perfringens on beef jerky were developed as a function of temperature (10, 17, 25, and $35^{\circ}C$). Risk of C. perfringens due to the consumption of beef jerky was estimated with @RISK and FDA-iRISK. The probability of foodborne illness due to C. perfringens through consumption of seasoned, vacuum packed beef jerky was estimated to be $2.77{\times}10^{-16}$ per person per day. Overall, the risk of contamination of beef jerky with C. perfringens is very low.