Browse > Article
http://dx.doi.org/10.3746/jkfn.2002.31.3.433

The Changes on Total Bacterial Counts and Coliform Counts of Human Milk vs Formula Milk Stored under Cold and Room Temperature Conditions  

Lee, Jo-Yoon (중부대학교 생명과학부)
Bae, Hyoung-Churl (충남대학교 동물자원학부)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.31, no.3, 2002 , pp. 433-437 More about this Journal
Abstract
Human milk is normally contaminated with various microorganisms, which seem to produce no ill effects. A contamination of hand-expressed and pump-expressed human milk is a major concern in the collection of storage milk. In this study we compared milks collected by two methods, hand expression and suction breast pump, to quantify differences in the degree of bacterial contamination. Thirty-one samples had been manually expressed. The mean of total bacterial counts was 10,600 CFU/mL (range: 360 ∼59,200 CFU/mL) and coliform counts was 43 CFU/mL (range: 20 ∼ 1,060 CFU/mL) in these samples. Whereas in the 118 breast pump-expressed samples, the mean of total bacterial counts was 20,200 CFU/mL (range: 240 ∼ 492,000 CFU/mL) and coliform counts was 158 CFU/mL (range: 4∼10,600 CFU/mL). There was no bacterial growth when the samples were incubated for 10 days at 4$\^{C}$. We also compared total bacterial growth in colostrum and in matured human milk for 24 hr at 20$\^{C}$ and 30$\^{C}$. Although bacterial growth had not shown for 24 hr at 20$\^{C}$, but shown slight growth in colostrum and rapidly increase in matured human milk for 24 hr at 30$\^{C}$. The coliform bacteria in all samples, particulary in formula milk, had grown at 20$\^{C}$ and 30$\^{C}$.
Keywords
pump-expressed human milk; manually-expressed human milk; bacterial contamination; human milk banking;
Citations & Related Records
연도 인용수 순위
  • Reference
1 American Academy of pediatrics. 1980. Committee on Nutrition : Human milk banking. Pediatrics 65: 854-857
2 Williams FH, Pittard WB. 1981. Human milk banking: Practical concerns for feeding premature infants. J Am Diet Assoc 79: 565-568.
3 Ford JE, Law BA, Valerie ME, Marshall VME. 1977. Influence of heat treatment of human milk on some of its protective constituents. J Pediatr 90: 29-34
4 Maria RG, Marwick K, McClelland DBL. 1977. Antimicrobial proteins in sterilised human milk. Br Med J 1: 12-14   DOI
5 Sauve R, Buchan K, Clyne A, Mclntosh D. 1984. Mother milk banking: Microbial aspects. Can J Public Health 75: 133- 136
6 Canadian Paediatric Society, Nutrition committee. 1985. Statement on human milk banking. Can Med Assoc J 132: 750-752
7 John TM. 1979. Human milk-Tables of the antimicrobial factors and microbiological contaminants relevant to human milk banking. Proceedings of Breast Feeding: The Natural Advantage Conference. October, Sydney
8 Wels JK, May JT. 1979. Anti-infective properties of breast milk. J Pediatr 94: 1-9   DOI
9 PittardⅢ WB, Diane MA, Edward RC, Bernard B. 1985. Bacteriostatic qualities of human milk. J Pediatr 107: 240- 243   DOI
10 Randall MG, Charles WD, Thomas BA, Edna SA, Cutberto G, Armond SG. 1984. Rapid high-temperature treatment of human milk. J Pediatr 104: 380-385   DOI
11 Myron L, Norman JL, Maria TA, Philip S. 1978. Comparison of bacterial contamination with two methods of human milk collection. J Pediatr 92: 236-237   DOI
12 Charles LP, Clarene CC. 1979. Survival of human milk leukocytes. J Pediatr 94: 61-64   DOI
13 Bengt B, Lars GB, Peter DC, Bo F, Seif G, Olle H. 1981. Collecting and banking human milk: To heat or not to heat? Br Med J 281: 765-769   DOI
14 Maria TA, James RH. 1979. Reduction of bacterial contamination in banked human milk. Brief Clinical and Lab Observation 95: 993-994