• Proceedings of the KSPS conference
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• 1996.10a
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• pp.390-398
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• 1996

Acoustic analysis study was performed on 20 normal subjects by speaking nonsense syllables composed of Korean bilabial stops(/p, $p^{*}$/, ph/) and their Preceding and/or following vowel /a/(that is, [pa, $p^{*}a$, pha, apa, $ap^{*}a$, apha]) with an ultraminiature pressure sensor in their mouths. Speech materials were phonated twice, once with a moderate voice, another time with a loud voice. The acoustic signal and intraoral pressure were recorded simultaneously on computer. By these procedures, we were to measure the intraoral pressure, closure duration and VOT of Korean bilabial stops, and to compare the values one another according to the intensity of phonation and the position of the target consonants. Intraoral pressure was measured by the peak intraoral pressure value of its wave; closure duration by the time interval between the onset of intraoral pressure build-up and the burst meaning the release of closure; Voice onset time(VOT) by the time interval between the burst and the onset of glottal vibration. Heavily aspirated bilabial stop consonant /ph/ showed the highest intraoral pressure value, unaspirated /p$^{*}$/, the second, slightly aspirated /p/, the lowest. The syllable initial bilabial stops showed higher intraoral pressure than word initial stops, and the value of loudly phonated consonants were higher than moderate consonants. The longest closure duration period was that of /$p^{*}$/ and the shortest, /p/, and the duration was longer in word initial position and in the moderate voice. In VOT, the order of the longest to shortest was /ph/, /p/, /$p^{*}$/, and the value was shorter when the consonant was in intervocalic position and when it was phonated with a loud voice.

• Journal of the Korean Applied Science and Technology
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• v.29 no.4
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• pp.552-560
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• 2012

Drug delivery technologies are patent protected formulation technologies that modify drug release profile, absorption, distribution, and elimination for the benefit of improving product efficacy and safety, as well as patient convenience and compliance. The most commonly used transdermal system is the skin patch using various types of technologies. Compared with other method of dosage, it is possible to use for a long term. It is also possible to stop the drug dosage are stop if the drug dosage lead to side effect. Polysaccharide, such as karaya gum and locust bean gum(LBG)/water-soluble chitosan oligomer(WSCO) were selected as base materials of TDS. Also, these polymers were characterized in terms of enhancers, tacrine contents. Among these polysaccharide, the permeation rate of karaya gum matrix was fastest in tacrine such as lipophilic drug in vitro. We used glycerin, PEG 400, and PEG 800 as enhancers. Therefore, transdermal absorption of tacrine could be improved by changing vehicle composition or by using penetration enhancers. Especially it would be anticipated that the high permeation efficacy could be obtained by using vehicle that has enhancing effect for itself and by adding enhancers to it.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.7 no.1
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• pp.50-55
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• 1996

Acoustic analysis study was performed on 20 normal subjects by speaking nonsense syllables composed of Korean bilabial stops$(/P, P^{\star}, P^{h}/)$ and their preceding and/or following vowel /a/ (that is, $[pa, p^{\star}a, p^{h}a, apa, ap^{\star}a, ap^{h}a]$) with an ultraminiature pressure, sensor. in their mouths. Speech materials were phonated twice, once with a moderate voice, another time with a loud voice. The acoustic signal and intraoral pressure were recorded simultaneously on computer. By these procedures, we were to measure the intraoral pressure, closure duration and VOT of Korean bilabial stops, and to compare the values one another according to the intensity of phonation and the position of the target consonants. Intraoral pressure was measured by the peak intraoral pressure value of Its wave closure duration by the time interval between the onset of intraoral pressure build-up and the burst meaning the release of closure ; Voice onset time(VOT) on by the time interval between the burst and the onset or glottal vibration. Heavily aspirated bilabial stop consonant /$p^h$/ showed the highest intraoral pressure value, unaspirated /$p^{\star}$/, the second, slightly aspirated /P/, the lowest. The syllable initial bilabial stops showed higher intraoral pressure than word initial stops, and the value of loudly phonated consonants were higher than moderate consonants. The longest closure duration period was that of /$p^{\star}$/ and the shortest, /P/, and the duration was longer in word initial position and in the moderate voice. In VOT, the order of the longest to shortest was $/{p^h}/, /p/, /{p^\star}/$, and the value was shorer when the consonant was in intervocalic position and when it was phonated with a loud voice.

• Phonetics and Speech Sciences
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• v.2 no.4
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• pp.119-127
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• 2010

Recently, we have launched a large-scale articulatory study to investigate how the three-way contrastive stops (i.e., lenis, fortis, and aspirated) in Korean are kinematically expressed (i.e., in terms of articulatory movement characteristics) in various contexts, using a magnetometer (Electromagnetic Articulography). In this paper, we report some preliminary results about how the three-way bilabial series /p,$p^h,p^*$/ produced in /Ca/ context in isolation are kinematically characterized not only during the lip closure but also during the following vocalic articulation. Some important notes could be made from the results. First, the degree of lip constriction (as measured by the lip aperture between the upper and lower lips) was smaller for the lenis /p/ and larger for the fortis/aspirated /$p^*,p^h$/, showing a two-way distinction during the closure. Second, the tongue lowering for the following vowel was more extreme after the lenis /p/ than after the fortis/aspirated /$p^*,p^h$/. Regarding this vocalic articulatory difference in the tongue height, we discussed the possibility that the articulatory tension associated with the fortis/aspirated stops is further reflected in the lingual vocalic movement maintaining the tongue position to a certain level for the following vowel /a/, while the lenis consonant does not impose such articulatory constraints, resulting in more tongue lowering. Finally, the temporal relationship between the release of the stop closure and the lowest tongue position of the following vowel remained constant, suggesting that CV coordination is invariantly maintained across the consonant type. This pattern was interpreted as supporting the view that the consonant and vowel gestures are coordinated in much the same way across languages.

• Phonetics and Speech Sciences
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• v.14 no.1
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• pp.1-11
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• 2022

The purpose of this study is to investigate the difference of vowel duration due to the voicing of word-final consonants in English and its relation to the types of word-final consonants (stops vs. fricatives), (partial) devoicing, and stop releasing. Addtionally, this study attempts to interpret the findings from the functional view that the vowels before voiced consonants are produced with a longer duration in order to enhance the salience of the voicing of word-final consonants. This study conducted a recording experiment with English native speakers, and measured the vowel duration, the degree of (partial) devoicing of word-final voiced consonants and the release of word-final stops. First, the results showed that the ratio of the duration difference was not influenced by the types of word-final consonants. Second, it was revealed that the higher the degree of (partial) devoicing of word-final voiced consonants, the longer vowel duration before word-final voiced consonants, which was compatible with the prediction based on the functional view. Lastly, the ratio of the duration difference was greater when the word-final stops were uttered with the release compared to when uttered without the release, which was not consistent with the functional view. These results suggest that it is not sufficient enough to explain the voicing effect by its function of distinguishing the voicing of word-final consonants.

• The Journal of Korean Medicine
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• v.28 no.4
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• pp.148-157
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• 2007

Background & Objective: Articular cartilage is a potential target for drugs designed to inhibit the activity of matrix metalloproteinases (MMPs) to stop or slow the destruction of the proteoglycanand collagen in the cartilage extracellular matrix. The purpose of this study was to investigate the effects of Cinnamomum cassia in inhibiting the release of glycosaminoglycan (GAG), the degradation of collagen, and MMP activity in rabbit and human articular cartilage explants. Methods: The cartilage-protective effects of Cinnamomum cassia were evaluated by using glycosaminoglycan degradation assay, collagen degradation assay, colorimetric analysis of MMP activity, measurement of lactate dehydrogenase activity and histological analysis in rabbit cartilage explants culture. Results: Interleukin-1a (IL-1a) rapidly induced GAG, but collagen was much less readily released from cartilage explants. Cinnamomum cassia significantly inhibited GAG and collagen release in a concentration-dependent manner. Cinnamomum cassia dose-dependently inhibited MMP-1, MMP-3 and MMP-13 activities from IL-1a-treated cartilage explants culture when tested at concentrations ranging from 0.02 to 1 mg/ml. Conclusion : These results indicate that Cinnamomum cassia inhibits the degradation of proteoglycan and collagen through the down regulation of MMP-1, MMP-3 and MMP-13 activities of IL-1a-stimulated rabbit and human articular cartilage explants.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.19 no.8
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• pp.788-794
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• 2009

Various types of latch designs for hard disk drives using load/unload mechanism have been introduced to protect undesired release motions of a voice coil motor(VCM) actuator from sudden disturbances. Recently, various inertia-type latches have been widely used because locking performance is better than that of other types of latch. However there has been a limit in the inertia type in order to guarantee perfect latch and unlatch operations because of changes in latch/unlatch conditions due to mechanical tolerance and temperature-dependent friction. In this paper, a reliable and robust magnetic latch mechanism is proposed through only simple modifications of coil and yoke shapes in order to overcome the mechanical limit of current inertia-type latches. This new magnetic latch does not have only a simple structure but it also ensures reliable operations and anti-shock performance. The operating mechanism of the proposed latch is theoretically analyzed and optimally designed using an electromagnetic simulation.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2001.06a
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• pp.83-89
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• 2001

Recent advances in the structural and molecular biology uncovered that a set of translation factors resembles a tRNA shape and, in one case, even mimics a tRNA function for deciphering the genetic ：ode. Nature must have evolved this 'art' of molecular mimicry between protein and ribonucleic acid using different protein architectures to fulfill the requirement of a ribosome 'machine'. Termination of protein synthesis takes place on the ribosomes as a response to a stop, rather than a sense, codon in the 'decoding' site (A site). Translation termination requires two classes of polypeptide release factors (RFs)： a class-I factor, codon-specific RFs (RFI and RF2 in prokaryotes; eRFI in eukaryotes), and a class-IT factor, non-specific RFs (RF3 in prokaryotes; eRF3 in eukaryotes) that bind guanine nucleotides and stimulate class-I RF activity. The underlying mechanism for translation termination represents a long-standing coding problem of considerable interest since it entails protein-RNA recognition instead of the well-understood codon-anticodon pairing during the mRNA-tRNA interaction. Molecular mimicry between protein and nucleic acid is a novel concept in biology, proposed in 1995 from three crystallographic discoveries, one, on protein-RNA mimicry, and the other two, on protein-DNA mimicry. Nyborg, Clark and colleagues have first described this concept when they solved the crystal structure of elongation factor EF- Tu：GTP：aminoacyl-tRNA ternary complex and found its overall structural similarity with another elongation factor EF-G including the resemblance of part of EF-G to the anticodon stem of tRNA (Nissen et al. 1995). Protein mimicry of DNA has been shown in the crystal structure of the uracil-DNA glycosylase-uracil glycosylase inhibitor protein complex (Mol et al. 1995; Savva and Pear 1995) as well as in the NMR structure of transcription factor TBP-TA $F_{II}$ 230 complex (Liu et al. 1998). Consistent with this discovery, functional mimicry of a major autoantigenic epitope of the human insulin receptor by RNA has been suggested (Doudna et al. 1995) but its nature of mimic is. still largely unknown. The milestone of functional mimicry between protein and nucleic acid has been achieved by the discovery of 'peptide anticodon' that deciphers stop codons in mRNA (Ito et al. 2000). It is surprising that it took 4 decades since the discovery of the genetic code to figure out the basic mechanisms behind the deciphering of its 64 codons.

• Journal of Periodontal and Implant Science
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• v.29 no.3
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• pp.507-519
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• 1999

The ultimate objective of periodontal treatment is to stop disease progression and to regenerate destroyed periodontal tissues and thereby regain normal function. Growth factors are naturally found polypetides which stimulate many cellular activities pertaining to wound healing by acting as signal molecule in controlling cell movement, proliferation, and matrix production. Platelet derived growth factor (PDGF) is 28,000-35,000 Da molecular weight dimeric protein with 2 long positively charged polypeptide chains connected by sulfide bonds. The purpose of this study is to evaluate histologically the initial guided tissue regeneration in a periodontal defect f a beagle dog treated with a biodegradable membrane formed with polylactic acid (poly-L-lactic acid) and polyglycolic acid loaded with 200ng/$cm^2$ platelet derived growth factor. 2 beagle dogs were used in he experiment. $5mm{\times}6mm$ alveolar bone defect was formed in upper and lower canines and third premolars and a reference notch was placed. PDGF-BB non-containing membrane was used as control. Each defect was randomly assigned to the test roup or the control group. The dogs were sacrificed 3 weeks after membrane placement. Toluidine blue and multiple staining was done for histological analysis. In the 3 week specimen in the control group, no new one formation could be seen. Small amount f bone resorption below the notch could be seen. In the notch, loose connective tissue with infiltration of inflammatory cells could be seen. Also thin discontinuous new cementum could be seen and the membrane still retained its structure. Where PDGF-BB containing membrane was used, new bone formation could be seen in the notch at weeks and also continuous thin cementum could be seen. PDL cells were observed between new bone and new cementum and some were attached to bone and cementum. These results suggest that new bone and cementum formation seen when PDGF-BB loaded membrane was used was due to inhibition of downgrowth of epithelial cells and also due to continuous release of the growth factor. Further study on the resorption characteristics of the membrane nd the release characteristics of the PDGF-BB is necessary. Also, development of a membrane easier to use clinically is necessary.

• Biomolecules & Therapeutics
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• v.18 no.2
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• pp.184-190
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• 2010

Clinical cases of pure red cell aplasia (PRCA) have been reported during the recombinant human erythropoietin (EPO) therapy for the anemia patients. PRCA is a rare hematological disorder leading to a severe anemia due to an almost complete stop of red blood cell production. Antibody (Ab)-associated PRCA is caused by the EPO-neutralizing Abs that eliminate the biological activity of EPO. In order to detect anti-EPO Abs in human sera, we performed conventional ELISA, directly coated bridging ELISA, and streptavidin coated bridging ELISA, and compared their sensitivity and specificity. Some false positive results were obtained in the conventional ELISA. One positive sample was detected successfully by streptavidin coated bridging ELISA, which was not appeared in the directly coated bridging ELISA. In conclusion, streptavidin coated bridging ELISA was substantially sensitive and specific format and one out of sixty-eight serum samples was proved to be anti-EPO positive.