• Journal of Dairy Science and Biotechnology
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• 제35권2호
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• pp.93-97
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• 2017

Linum usitatissimum L. (flaxseed) is emerging as an important functional food ingredient because of its rich contents, namely, ${\alpha}$-linolenic acid (ALA, omega-3 fatty acid), lignans, and fiber, which are potentially beneficial for human health. Furthermore, flax or flaxseed oil has also been incorporated as a functional food ingredient into various foods such as milk, dairy products, and meat products. Flaxseed is known to possess antimicrobial activity in vitro and in vivo, but its growth-stimulating effect on lactic acid bacteria is not clear. Hence, the objective of this study was to determine whether crude flaxseed extract stimulated the growth kefir-isolated lactic acid bacteria in vitro. The result of this study showed that Lactobacillus kefiranofaciens DN1, Lactobacillus brevis KCTC3102, Lactobacillus bulgaricus KCTC3635, and Lactobacillus plantarum KCTC3105 treated with $100{\mu}L$ of crude flaxseed extract showed significantly higher growth than the control treated with $100{\mu}L$ of water (p<0.05). Based on the results of this study, crude flaxseed extract could be used as a growth stimulator for lactic acid bacteria in various food applications, including production of milk and dairy products.

• 한방재활의학과학회지
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• 제23권3호
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• pp.107-116
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• 2013

Objectives The purpose of this study was to evaluate the most effective stimulating electrode in Microcurrent electrical neuromuscular stimulation (MENS) for pain relief, and to apply to the treatment of Delayed onset muscle soreness (DOMS). Methods We included 45 participants who met the inclusion criteria. DOMS of both Triceps Surae Muscles were experimentally induced through eccentric contractions. 24-hours after induction of DOMS, who scored more than 40 mm in visual analogue scale (VAS) were randomly assigned into three groups (n=44). In 15 of them, needle electrodes were inserted into BL57, BL56. In 15 of them, surface electrodes were applied on the same points and the others were treated with manual acupuncture. The effects of pain relief were assessed by visual analogue scale (VAS), mechanical pain threshold (MPT), surface electromyography (SEMG). Results In VAS, group comparison had no significant difference after all treatments had done. The difference in VAS between the time before the treatment was started (Day 2) and after all treatments were completed was greater in Acupuncture group than that of needle-electrode group. In MPT, there was no significant difference among groups. Electrical contraction decreased significantly at 2nd before-after treatment comparison in needle-electrode MENS group. But fatigue scores were not significantly different between groups. Conclusions The results suggest that the types of electrodes has no significant effect on microcurrent therapy in DOMS.

• 정보처리학회논문지B
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• 제19B권2호
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• pp.77-82
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• 2012

본 논문에서는 다양한 생체신호 중 영상 및 음성 신호에 대한 분석 파라메터들을 적용한 실험 방법을 통해 폐 수혈 상응점 자극에 따른 효과성을 입증하는 연구를 수행하였다. 이를 위해 20대 남성 25명을 대상으로 폐와 연관된 수혈 상응점 자극 전과 후의 얼굴 영상 및 음성을 수집하였다. 또한, 수집된 자료를 기반으로 한의학적 진단 이론에서 제시하고 있는 폐와 관련된 우측 뺨 영역의 색상 변화와 음성 에너지 크기 및 발화속도의 변화를 측정, 분석하였다. 결과적으로 폐 수지침 요법을 수행한 후에 우측 뺨 영역의 L값이 평균 2.33감소하였으며 a값과 b값이 평균 0.76, 0.97증가하였다. 또한, 음성에너지 크기는 평균 0.42증가하였으며 발화속도는 평균 0.07감소하였다. 즉, 폐 수지침 요법을 통해 폐 기능이 향상되는 효과를 나타낸 것으로 분석되었다.

• 환경생물
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• 제17권1호
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• pp.11-15
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• 1999

저선량 방사선에 의한 식물 유익효과를 구명하기 위하여 2개 종묘회사로부터 4년간 묵은 종자를 분양받아 저수준의 ${\gamma}$선을 조사하여 온실과 포장에서 재배하여 그 생육 상황을 조사하였다. 서울종묘의 한여름배추는 0.5 Gy 조사구를 제외하고는 발아율 증대효과는 없었고 8 Gy와 12 Gy 조사구에서 유묘초장과 생체중이 각각 평균 20%와 40%정도 증가하여 초기생육 촉진효과는 있었으나 포장수량 증가 효과는 볼 수 없었다. 흥농종묘의 tropic emperor 품종에서는 1 Gy 조사에서 발아율이 10% 정도 증가하였고 유묘초장과 생체중은 1 Gy와 12 Gy 조사구에서 평균 20% 정도 증가하였으며 포장수량에서도 0.5 Gy와 4 Gy에서 초장과 생체중이 각각 10%와 20%정도 증가하였다. 결과적으로 저선량 방사선에 의한 배추의 초기생육 촉진효과가 있는 것으로 사료된다.

• Molecules and Cells
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• 제37권8호
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• pp.628-635
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• 2014

2-(Trimethylammonium) ethyl (R)-3-methoxy-3-oxo-2-stearamidopropyl phosphate [(R)-TEMOSPho], a derivative of an organic chemical identified from a natural product library, promotes highly efficient megakaryopoiesis. Here, we show that (R)-TEMOSPho blocks osteoclast maturation from progenitor cells of hematopoietic origin, as well as blocking the resorptive function of mature osteoclasts. The inhibitory effect of (R)-TEMOSPho on osteoclasts was due to a disruption of the actin cytoskeleton, resulting from impaired downstream signaling of c-Fms, a receptor for macrophage-colony stimulating factor linked to c-Cbl, phosphoinositol-3-kinase (PI3K), Vav3, and Rac1. In addition, (R)-TEMOSPho blocked inflammation-induced bone destruction by reducing the numbers of osteoclasts produced in mice. Thus, (R)-TEMOSPho may represent a promising new class of antiresorptive drugs for the treatment of bone loss associated with increased osteoclast maturation and activity.

• 한국산학기술학회논문지
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• 제14권6호
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• pp.2812-2818
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• 2013

한의학과 대체의학은 그 뛰어난 진단 기술 및 처방과 치료 효과에도 불구하고 선호도에 있어 서양의학에 많이 떨어지고 있는 것이 현 실정이다. 마찬가지로 한의학과 밀접히 연계되어 있는 대체의학도 그 효과에 대한 입증이 과학적으로 규명이 되지 못하고 있는 것이 가장 큰 단점으로 여겨진다. 본 논문에서는 대체의학에서 흔히 사용하는 이침 요법이 실제 인체 장기 기능 강화에 도움이 되는 행위인지를 생체신호를 분석하는 방법 중 얼굴의 피부색 변화를 살펴보는 망진 이론을 적용하여 이를 규명하는 방법을 제안하고자 한다. 이를 위해 폐 이혈 반사점을 자극한 후의 우측뺨 색상을 측정하여 자극 전과의 비교, 분석 실험을 수행하였다. 또한 검증과 규명을 위해 우선적으로 인체가 폐에 미치는 영향의 분석에 필요한 영상 분석 요소를 파악하였다. 실험 결과, 폐 이혈 반사점 자극에 의해 우측뺨의 a값과 b값이 증가하는 결과가 도출되었다.

• 대한한방내과학회지
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• 제15권1호
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• pp.80-98
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• 1994

According to the original documents, Sagungsan is considered as an effective drug for controlling the hypertensive epistaxis induced by tension of autonomic nerve and it's hyperfunction. The present experiment was designed to understand the effect of Sagungsan extract on the hemostatic action, intracranial pressure, blood pressure and cardiovascular system in experimental animals. And thus the bleeding time, prothrombin time, capillary dilation, blood pressure, Intracranial pressure, and enzymatic analysis of the ATPase activities were studied. The result obtained here were as followings: 1. Sagungsan water extract reduced the bleeding time in mouse, and prolonged the prothrombin time in rabbits. 2. The drug extract increased the tail volume by capillary dilation in rats. 3. The drug extract inhibited the increase of intracranial pressure and arterial blood pressure in rabbits. 4. At the early time, the increase of arterial blood pressure by the drug extract significantly inhibited by pretreated atropin and regitine in rabbits. 5. The drug extract relaxed the smooth muscle by stimulating the Mg2+-Ca2+-ATPase activities of gastric sarcoplasmic reticulum isolated from rabbit stomach. 6. The drug extract stimulated the heart contraction by inhibiting the $Mg^{2+}-Ca^{2+}-ATPase$ activities of cardiac sarcoplasmic reticulum isolated from rabbit heart. The inhibitory mechanism was reversible and noncompatitive. 7. The drug extract increased the hepatic blood volume by stimulating the hepatic total ATPase activities and hepatic metabolism. 8. The drug extract acted as a tranquilizer by inhibiting the neural Na+-K+-ATPase activity. According to the results, Sagungsan water extract dilated the capillaries, stimulated the heart beat, and thus increased the blood flow with decreasing the intracranial pressure and blood pressure. These effects stanches the epistaxis collectively.

• 아시안잔디학회지
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• 제2권1호
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• pp.41-48
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• 1988

Research was conducted to obtain easy and secure methods for promoting germination of Korean lawngrass (Zoysia japonic a Steud.) seed. The effect of sodium hypochiorite (NaOCl) treatment on germination of seeds was compared with that of potassium hydroxide (KOH) treatment under conditions of duration of seed storage after harvest and temperature of treatment. Emergence of seedlings from soil and micro-flora infection rate among seed treatments were investigated. 1. The promotion of germination was much more evident in seeds treated with 4% NaOCl for 8 and 10 hours than in seeds treated with 25% or 30% KOH for 30 and 40 minutes. Longer durations of storage before seed treatment in KOH than in NaOCl were required to initiate enough germination. 2. Differences in temperature of treatment with NaOCT did not have much influence on germination of seeds and treatment temperature of 15˚C was better than that of 200˚C and 300˚C for promoting germination. But seed treatment with KOH significantly promoted germination with increasing temperature of treatment from I 5˚C to 20˚C and 30˚C. 3. GA$_3$ treatment enhanced germination in NaOCl-pretreated seeds at early stage of imbibition and in-creased about 10% germination after 10 days of imbibition in KOH-pretreated seeds. 4. NaOC1 treatments significantly decreased the rate of the infection of microflora in seed samples and enhaced emergence of seedlings from soil compared with KOH treatment. 5. NaOC1 treatment had advantage over KOH treatment with respect to ease of preparing, securing and handling in stimulating germinating of Korean lawngrass seeds.

• 한국식품과학회지
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• 제35권4호
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• pp.702-707
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• 2003

본 연구는 가시오가피를 함유한 성장촉진용 조성물인 GSM의 성장촉진 효과를 관찰하기 위하여, 족경골 말단의 성장판 증식과 골성장인자 IGF-1 유전자 발현에 미치는 영향, 골세포와 간세포에서 골성장인자 IGF-1 유전자 발현, 그리고 족경골의 생육 효과를 관찰하였다. GSM은 세포실험을 통하여 주요 성장 인자인 IGF-1을 간에서 합성을 촉진시킬 뿐만 아니라, 골에서도 합성을 촉진시키는 효과를 나타내었다. 그리고 간과 골에서 합성이 촉진된 IGF-1은 동물실험을 통하여 장골 길이 성장의 중요한 척도가 되는 성장판 말단부의 증식층 및 비대층의 연골세포 활력을 증대시켜 성장판 두께를 증가시켰고, 또한 골무기질밀도를 증가시키는 효과를 보여주었다. 따라서 본 연구는 GSM의 지속적인 연구를 통하여 성장기 어린이에게 성장촉진 및 골강화에 응용할 수 있는 소재로 개발 가능성이 있음을 제시하였다.

• Animal cells and systems
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• 제16권3호
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• pp.207-214
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• 2012

Burns are one of the most devastating forms of trauma and wound healing is a complex and multicellular process, which is executed and regulated by signaling networks involving numerous growth factors, cytokines, and chemokines. Recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF) was specifically produced from rice cell culture through use of a recombinant technique in our laboratory. The effect of rhGM-CSF on promotion of deep second-degree burn wound healing on the back skin of a hamster model was evaluated through a randomized and double-blind trial. As macroscopic results, hamster skins of the experimental groups showed earlier recovery by new epidermis than the control groups. Immunohistochemical reactions of proliferating cell nuclear antigen and transforming growth factor-b1, which are indicators of cell proliferation, were more active in the experimental group, compared with the control group. On electron microscopy, basal cells in the epidermis of the experimental group showed oval nuclei, prominent nucleoli, numerous mitochondria and abundant free ribosomes. In addition, fibroblasts contained well-developed rough endoplasmic reticulum with dilated cisternae. Bundles of collagen fibrils filled the extracellular spaces. Particularly, ultrastructural features indicating active metabolism for regeneration of injured skin at 15 days after burn injury, including abundant euchromatin, plentiful free ribosomes, and numerous mitochondria, were observed. These findings suggest that use of rhGM-CSF could result in accelerated deep second-degree burn wound healing in animal models.