• Archives of Pharmacal Research
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• 제6권2호
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• pp.123-131
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• 1983

Polysaccharide fractions were prepared from Ginseng root, Mori Radicis Cortex (M. R. C. ), Phellodendri Cortex (Ph. C. ), Sappan Wood (S. W. ) and Tigli Semen (T. S.). Water extract was also prepared from the mixture of ph. C., S. W. and T. S. Ginseng polysaccharide and water extract of the mixture showed marked antitumor activity against sarcoma 180. Ginseng polysaccharide showed a mild increasing effect on the number of circulating leucocytes and a marked increasing effect on the number leucocytes and a marked increasing effect on the number of plaque forming cells (PEC). Polysaccharides from ginsing root, S. W., Ph. C. + T. S. and water extract of the mixture showed dramatic inducing activities of periotoneal exudate cells (PEC), polymorphonuclear leucocytes (PMN) and macrophages. These results suggest the possibility that water extract of the mixture may have the lentinan like effect and ginseng polysaccharide may have stimulating effects on the general immune system.

• 농업과학연구
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• 제47권3호
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• pp.445-457
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• 2020

Prebiotics are defined as "Nondigestible food ingredients that beneficially affect the host by selectively stimulating the growth and activity of bacteria in the intestine" and as defined improve host health. This study was carried out to investigate the effects of bifidobacteria (Bifidobacterium lactis BB12 and Bifidobacterium longum BB536) growth enhancer (BE0623) supplement as a prebiotic. The addition of BE0623, a growth promoting material for bifidobacteria, significantly increased bifidobacteria viable cells counts in fermented milk by about 45 to 75 times compared to the non-added control group. In addition, microscopic observation showed a significant effect on proliferation of bifidobacteria in fermented milk with added BE0623. The viable cell counts in bifidobacteria also increased roughly 102-fold compared to the control group (non-added BE0623) and was higher than that of commercial growth promoters. Each fraction obtained though the purification of BE0623 influenced the increase of bifidobacteria growth. Culturing bifidobacteria with a combination of fractions of BE0623 had a synergistic effect compared to culturing bifidobacteria with each fraction individually. When any of the fractions were not added, the effect of the growth enhancer on bifidobacteria was reduced. These results indicate that all fractions contain substances that promote the growth of bifidobacteria. Therefore, BE0623 is considered to be available as a growth promoting material for bifidobacterium.

• Food Science and Biotechnology
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• 제18권3호
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• pp.706-711
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• 2009

Lysozyme was treated with digestive enzymes and the production of interleukin 8 (IL-8) was measured in Caco-2 cell with the peptides from lysozyme upon stimulating with lipopolysaccharide (LPS) to investigate the overall anti-inflammatory activity of lysozyme when it is in digestive tracts. Lysozyme reduced IL-8 production, and the peptides from pepsin hydrolysis of lysozyme had the similar effect. The products of trypsin digestion of lysozyme had no effect on the reduction of IL-8 production while those of pepsin-trypsin hydrolysis did. The effectiveness of lowering IL-8 production was not different by time of the peptide addition. When Caco-2 cells were pre-incubated with peptides for 24 hr, the reduction effects were observed from the peptides from pepsin hydrolysis, indicating that some of the peptides are still remaining in the cells. Therefore, it can be concluded that the IL-8 reduction effect of lysozyme against LPS still remained even after the pepsin and trypsin hydrolysis.

• KSBB Journal
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• 제23권2호
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• pp.164-168
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• 2008

본 연구를 통하여 요즘 기능성 측면에서 관심을 끌고 있는 참죽나무 잎의 열수추출물과 유기용매 추출물을 이용하여 항산화능과 면연증강능을 확인하였다. 열수 추출물의 총 폴리페놀 함량은 46.5-59.6 mg/100 g으로 유기용맹 추출물보다 그 수치가 더 높았다. 열수 추출물과 유기용맹 추출물의 항산화 활성은 2,2-diphenyl-picryl-hydraryl (DPPH) radical 소거활성으로 6-33%로 기준물질보다 낮은 값을 나타내었다. 참죽나무 잎 추출물의 면역증강능을 확인하기 위하여 박테리아 감염에 대항하는 숙주방어체계에 필요한 NO 생성능을 확인하였다. 열수 추출물은 마크로파지 세포주인 RWA 264.7의 NO 생성을 증가시켰다. 그러나 유기용매추출물은 별다른 변화를 가져오기 않았다. 항염효과를 증명하기 위하여 인지질다당류 (LPS) 유발 NO생성에 미치는 영향을 조사하여, NO 생성을 억제하는 것을 증명하였다. 즉, 면역증강능의 확인 실험을 통하여 참죽나무 잎의 열수추출 분획에 대식세포의 활성을 증가시키는 성분이 포함되어 있고, 유기용매 추출 분획에서는 대식세포의 활성을 감소시키는 성분이 포함되어 있는 것으로 판단된다. 이상과 같은 결과를 미루어 보았을 때, 참죽이 한 가지 식물인데도 불구하고 유효 성분에 대한 추출 방법에 따라 상이한 면역 증강 및 항균, 항염 작용을 보이는 것으로 나타났다.

• Clinical and Experimental Reproductive Medicine
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• 제42권2호
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• pp.62-66
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• 2015

Objective: To evaluate the effect of a gonadotropin-releasing hormone (GnRH) antagonist protocol using corifollitropin alfa in women undergoing assisted reproduction. Methods: Six hundred and eighty-six in vitro fertilization-embryo transfer (IVF)/ intracytoplasmic sperm injection (ICSI) cycles were analyzed. In 113 cycles, folliculogenesis was induced with corifollitropin alfa and recombinant follicle stimulating hormone (rFSH), and premature luteinizing hormone (LH) surges were prevented with a GnRH antagonist. In the control group (573 cycles), premature LH surges were prevented with GnRH agonist injection from the midluteal phase of the preceding cycle, and ovarian stimulation was started with rFSH. The treatment duration, quality of oocytes and embryos, number of embryo transfer (ET) cancelled cycles, risk of ovarian hyperstimulation syndrome (OHSS), and the chemical pregnancy rate were evaluated in the two ovarian stimulation protocols. Results: There were no significant differences in age and infertility factors between treatment groups. The treatment duration was shorter in the corifollitropin alfa group than in the control group. Although not statistically significant, the mean numbers of matured (86.8% vs. 85.1%) and fertilized oocytes (84.2% vs. 83.1%), good embryos (62.4% vs. 60.3%), and chemical pregnancy rates (47.2% vs. 46.8%) were slightly higher in the corifollitropin alfa group than in the control group. In contrast, rates of ET cancelled cycles and the OHSS risk were slightly lower in the corifollitropin alfa group (6.2% and 2.7%) than in the control group (8.2% and 3.5%), although these differences were also not statistically significant. Conclusion: Although no significant differences were observed, the use of corifollitropin alfa seems to offer some advantages to patients because of its short treatment duration, safety, lower ET cancellation rate and reduced risk of OHSS.

• 농업과학연구
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• 제11권2호
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• pp.201-206
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• 1984

효과적(效果的)이고 용이(容易)하게 사용할 수 있는 한국잔디(Zoysia japonica Steud.) 종자(種子)의 발아(發芽) 촉진방법(促進方法)을 모색(摸索)코자 sodium hypochlorite(SH)용액(溶液)을 사용하여 종피처리(種皮處理)한 결과(結果)는 다음과 같다. 1. 종자(種子)를 4%의 SH용액(溶液)에 2시간(時間) 침지처리(浸漬處理)한 후(後) 8시간(時間) 수세(水洗)한 결과(結果) KOH처리(處理)에 비(比)하여 발아촉진효과(發芽促進效果)가 좋았다. 2. SH처리(處理) 후(後) 수세시간(水洗時間)에 따라서 발아율(發芽率)에 차이(差異)가 있었으나 대체로 4~8시간(時間)의 수세(水洗)가 가장 효과적(效果的)이었다. 3. SH처리(處理) 후(後) GA를 처리(處理)함으로써 발아(發芽)가 더욱 촉진(促進)되었으나 무처리구(無處理區)에서는 GA처리(處理)에 의한 발아촉진효과(發芽促進效果)가 인정(認定)되지 않았다. 4. SH처리(處理)는 KOH처리(處理)에 비(比)하여 균유(菌類)의 종자감염율(種子感染率)을 크게 감소(減少)시켰다. 5. SH처리(處理)는 한국잔디 종자(種子)의 발아촉진(發芽促進)을 위해서 KOH처리법(處理法)에 비(比)하여 쉽게 준비(準備)할 수 있고, 안정성(安定性)이 있으며, 취급(取扱)하기 쉬운 효과적(效果的)인 종피처리방법(種皮處理方法)이라고 생각된다.

• 한국수정란이식학회지
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• 제13권3호
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• pp.301-312
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• 1998

난포액내 함유되어 있는 단백질성분 중에서 sucrose 층으로부터 정자의 swim-up 이동을 자 극하는 성분을 분리하기 위하여 paper chromatography (PC) 및 reverse phase column (RPC) 과 superose column (SC)를 이용한 액체 chromatography의 분리효과를 조사하였던 바 결과는 다음과 같다. 1. Chromatography용 paper로 분리한 각 band 의 성분은 첨가농도가 증가할수록 정자의 이동과 운동을 자극하였으며, 특히 band 1 성분은 정자의 이동을 유의하게 증가시켰다. 그러나, 동일 첨가수준에서 bands 성분의 정자 이동과 운동자극효과는 난포액의 효과에 비하여 유의하게 낮았다. 2. $\mu$RPC를 이용 2~5mm 난포로 부터 분리한 성분중 RT3.33, RT7.00, RTl3.87 및 RTl6.6A 성분은 정자의 이동을 자극하였으나, 자극효과는 매우 적었다. 3. $\mu$RPC를 이용 10mm 난포로 부터 분리한 성분은 정자의 이동과 활력을 자극하지 않았다. 4. SC를 이용 2-5mm 난포로 부터 분리한 성분 중 RVI.35 성분과 RV0.82 성분은 정자의 이동과 운동을 유의하게 자극하였다. 결론적으로 난포액내 정자의 이동과 운동을 자극하는 단백질 성분은 superose column을 이용하여 효과적으로 분리할 수 있으며, 분리된 RVI.35 성분과 RV0.82 성분은 정자의 swim-up 분리를 자극하였다.

• 한국식품과학회지
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• 제35권3호
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• pp.488-492
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• 2003

S. cereviaiae KCTC 7911에 돌연변이를 유도하고 selective pressure로서 세포벽 분해효소인 zymolsae와 mechanical stress인 glass bead를 차례로 처리하여 효모변이주를 S. cerevisiae IS2를 선발하였다. S. cerevisiae IS2는 세포벽 분해효소인 zymolase의 농도별 내성실험 결과 wild-type에 비해 훨씬 강한 내성을 보여 세포벽에 변화가 일어난 균주로 예상된다. 효모변이주와 wild-type으로부터 베타글루칸을 추출하여 면역활성에 미치는 영향을 조사하기 위해 생쥐의 복강에 주사하고 생성되는 면역세포의 수, NO 생성능, 및 면역세포의 대다수를 차지하는 대식세포의 탐식능을 측정하였다. 베타글루칸을 쥐의 복강에 주사하였을 때 베타글루칸의 종류에 상관없이 면역세포의 수, NO 생성능 및 대식세포의 활성도가 증가하는 결과를 얻을 수 있었다. 특히 변이주 베타글루칸을 주사하였을 경우 wild-type 베타글루칸에 비해 면역세포의 수는 1.40배, NO 생성능은 1.12배, 대식세포의 활성도와 탐식능은 각각 1.18배와 1.43배 높은 수치를 얻을 수 있었다. 이러한 결과들로 미루어 변이주 베타글루칸이 wild-type 베타글루칸보다 우수한 면역활성 촉진능력을 가지고 있음을 증명할 수 있었으며, 고부가가치 기능성 면역물질로서의 응용 가능성을 확인할 수 있었다.

• 대한수의학회지
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• 제37권4호
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• pp.917-924
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• 1997

Estrus cycle of cow, season and follicldar stimulating treatment, having an effect on the number of follicle, are investigated for the oocyte recovery rate in ovum pick-up(OPU). The number of follicle aspirated and oocyte collected on the different days of estrus cycle(D 4~5, D 9~10 and D 14~15) were not significantly different among the groups. The higher number of viable oocytes were produced on Jan-May(79.0%) than Jun-Aug(33.3%) by OPU in cow. The number of follicle and aspirated oocyte in cows treated FSH or PMSG combined with bovine somatotropin(bST) were 1.2~1.5 times higher than in cows treated alone follicular stimulating hormone(FSH) or pregnant mare serum gonadotropin(PMSG). In conclusion, OPU can be repeatedly practiced 2 or 3 times in an estrus cycle. In addition, the high environmental temperature is not good for ovarian function of cow and the bST co-treatment with FSH or PMSG is increasing the number of aspiratable follicle.

• Asian-Australasian Journal of Animal Sciences
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• 제23권8호
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• pp.1049-1056
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• 2010

This study was conducted to investigate the effects of dietary glutamine (Gln) on the productive performance and egg quality of laying hens. A total of four hundred Lingnan Yellow laying hens aged 34 weeks were randomly assigned into four groups (100 laying hens/group), and fed, respectively, with diets supplemented with 0% (control group), 0.2%, 0.4%, and 0.8% Gln during the 6-week feeding period. The results were as follows. First, the productivity of laying hens fed with 0.8% Gln in diet was significantly increased (p<0.05); however, the egg quality (egg weight, yolk weight, shell weight, egg shape index, shell thickness, shell density, shell breaking strength, yolk color, yolk index, and Haugh unit) was not affected compared with that of the control group (p>0.05). Second, luteinizing hormone (LH) (p<0.01), follicle stimulating hormone (FSH) (p<0.01), triiodothyronine ($T_3$), and tetraiodothyronine ($T_4$) contents (p<0.05) in blood of laying hens fed with 0.8% Gln in diets were also significantly improved, and greater improvement in the duodenum and oviduct structure was observed in that treatment group. This study indicated for the first time that diets with 0.8% Gln were able to increase the productive performance of laying hens through stimulating hormone secretion and better development of both the duodenum and oviduct structure in laying hens.