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Study on Immuno-stimulating Activity of ${\beta}$-Glucan Isolated from the Cell Wall of Yeast Mutant Saccharomyces cerevisiae IS2  

Park, Jeong-Hoon (Research Institute, Bioprogen Co., Ltd.)
Kang, Man-Sik (Research Institute, Bioprogen Co., Ltd.)
Kim, Hong-Il (Department of Biotechnology, Faculty of Life Science, Konkuk University)
Chung, Bong-Hyun (Laboratory of Integrative Biotechnology, Korea Research Institute of Bioscience and Biotechnology)
Lee, Kwang-Ho (Department of Biotechnology, Faculty of Life Science, Konkuk University)
Moon, Won-Kuk (Research Institute, En-Bio technology Co., Ltd.)
Publication Information
Korean Journal of Food Science and Technology / v.35, no.3, 2003 , pp. 488-492 More about this Journal
Abstract
Yeast cell wall mutant, Saccharomyces cerevisiae IS2 was screened by the NTG treatment of Saccharomyces cerevisiae KCTC 7911. The mutant was highly resistant to zymolase, which specifically degrades ${\beta}$-1,3-D-glucose chain of ${\beta}$-glucan and mechanical disruption by glass beads. These phenomena demonstrate that the yeast mutant has cell wall structure different from the wild-type. The ${\beta}$-glucan of yeast mutant and wild-type strains was recovered by sequential extraction with NaOH. The injection of ${\beta}$-glucan into the abdominal cavity of mouse resulted in an increase in the number of peritoneal immune cells, NO (nitric oxide) production, and phagocytic activity of macrophage. The number of immune cells was found to be $3.90{\times}10^6\;cells/10\;mL$ and $5.48{\times}10^6\;cells/10\;mL$ with the wild-type and mutant ${\beta}$-glucan, respectively. The effect on the NO production and phagocytic activity of mutant ${\beta}$-glucan were 1.69 and 1.43-fold higher than those of wild-type. These results indicate that the immuno-stimulating activity of alternated ${\beta}$-glucan from mutant yeast is higher than that of wild-type.
Keywords
yeast cell wall mutant; ${\beta}$-glucan; NO production; phagocytic activity; immuno-stimulating activity;
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