• Journal of Dairy Science and Biotechnology
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• v.35 no.2
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• pp.93-97
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• 2017

Linum usitatissimum L. (flaxseed) is emerging as an important functional food ingredient because of its rich contents, namely, ${\alpha}$-linolenic acid (ALA, omega-3 fatty acid), lignans, and fiber, which are potentially beneficial for human health. Furthermore, flax or flaxseed oil has also been incorporated as a functional food ingredient into various foods such as milk, dairy products, and meat products. Flaxseed is known to possess antimicrobial activity in vitro and in vivo, but its growth-stimulating effect on lactic acid bacteria is not clear. Hence, the objective of this study was to determine whether crude flaxseed extract stimulated the growth kefir-isolated lactic acid bacteria in vitro. The result of this study showed that Lactobacillus kefiranofaciens DN1, Lactobacillus brevis KCTC3102, Lactobacillus bulgaricus KCTC3635, and Lactobacillus plantarum KCTC3105 treated with $100{\mu}L$ of crude flaxseed extract showed significantly higher growth than the control treated with $100{\mu}L$ of water (p<0.05). Based on the results of this study, crude flaxseed extract could be used as a growth stimulator for lactic acid bacteria in various food applications, including production of milk and dairy products.

• Journal of Korean Medicine Rehabilitation
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• v.23 no.3
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• pp.107-116
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• 2013

Objectives The purpose of this study was to evaluate the most effective stimulating electrode in Microcurrent electrical neuromuscular stimulation (MENS) for pain relief, and to apply to the treatment of Delayed onset muscle soreness (DOMS). Methods We included 45 participants who met the inclusion criteria. DOMS of both Triceps Surae Muscles were experimentally induced through eccentric contractions. 24-hours after induction of DOMS, who scored more than 40 mm in visual analogue scale (VAS) were randomly assigned into three groups (n=44). In 15 of them, needle electrodes were inserted into BL57, BL56. In 15 of them, surface electrodes were applied on the same points and the others were treated with manual acupuncture. The effects of pain relief were assessed by visual analogue scale (VAS), mechanical pain threshold (MPT), surface electromyography (SEMG). Results In VAS, group comparison had no significant difference after all treatments had done. The difference in VAS between the time before the treatment was started (Day 2) and after all treatments were completed was greater in Acupuncture group than that of needle-electrode group. In MPT, there was no significant difference among groups. Electrical contraction decreased significantly at 2nd before-after treatment comparison in needle-electrode MENS group. But fatigue scores were not significantly different between groups. Conclusions The results suggest that the types of electrodes has no significant effect on microcurrent therapy in DOMS.

• The KIPS Transactions:PartB
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• v.19B no.2
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• pp.77-82
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• 2012

We carried out study to prove effectiveness as stimulating corresponding points to lung in hand to experiment applied analysis parameters for image and audio signals in this paper. To this end we collected facial image and voice before and after stimulating corresponding points to lung in hand to a male 20s 25 people. In addition, we analyzed change color, voice energy and speaking rate of right cheek area corresponding points to lung to suggest the theory of the Oriental medicine diagnosis based on data collected. As a result, after performing hand acupuncture, L value of right cheek area decreased average 2.33 and a value b value increased 0.76, 0.97 on average. In addition, size of voice energy increased average 0.42, speaking rate decreased average 0.07. In other words, effect of lung function was improved using hand acupuncture corresponding points to lung.

• Korean Journal of Environmental Biology
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• v.17 no.1
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• pp.11-15
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• 1999

To investigate the stimulating effects of the low- dose radiation, the seeds of chinese cabbages (Brassica campestris L. cv. Hanyoreum and cv. Tropic emperor) were irradiated at the dose of 0.5 Gy~20 Gy with the ${\gamma}$-ray radition. In case of ‘Hanyoreum’cultivar, there were no significant promoting effects on the germination rate except the 0.5 Gy irradiated group. The increase in seedling height and fresh weight in 8 Gy and 12 Gy irradiated groups was 20% and 40%, respectively, which suggested the stimulating effect of the low-dose radiation on the early growth. In case of‘Tropic emperor’cultivar, the germination rate of 1 Gy irradiated group increased approximately 10% and the seedling height and fresh weight of 1 Gy and 12 Gy irradiated group in the greenhouse 20% on the average, and plant height and fresh weight of 0.5 Gy and 4 Gy irradiated group in the field increased 10% and 20% on the average, respectively.

• Molecules and Cells
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• v.37 no.8
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• pp.628-635
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• 2014

2-(Trimethylammonium) ethyl (R)-3-methoxy-3-oxo-2-stearamidopropyl phosphate [(R)-TEMOSPho], a derivative of an organic chemical identified from a natural product library, promotes highly efficient megakaryopoiesis. Here, we show that (R)-TEMOSPho blocks osteoclast maturation from progenitor cells of hematopoietic origin, as well as blocking the resorptive function of mature osteoclasts. The inhibitory effect of (R)-TEMOSPho on osteoclasts was due to a disruption of the actin cytoskeleton, resulting from impaired downstream signaling of c-Fms, a receptor for macrophage-colony stimulating factor linked to c-Cbl, phosphoinositol-3-kinase (PI3K), Vav3, and Rac1. In addition, (R)-TEMOSPho blocked inflammation-induced bone destruction by reducing the numbers of osteoclasts produced in mice. Thus, (R)-TEMOSPho may represent a promising new class of antiresorptive drugs for the treatment of bone loss associated with increased osteoclast maturation and activity.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.6
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• pp.2812-2818
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• 2013

Medicine such as Korean oriental medicine and alternative medicine is inferior to western medicine in diagnostic skills and treatment regimen but despite going through a lot of in western medicine preference to that is situations. Likewise, in Korean oriental medicine and alternative medicine have been closely linked to that effect not proven scientifically to be solved is regarded as the biggest drawback. In this paper, auricular therapy that is often used in alternative medicine to help strengthen the internal organs function which whether the conduct of stimulating ear is effective or not by applying to ocular inspection of Korean oriental medicine is proposed. For this purpose, lung ear reflex points to stimulate were performed after the right cheek color by measuring stimulation before comparison and analysis of the experiments. In addition, in order to examine this first analysis of the human body to the lungs and the elements of image analysis is to study. Experimental result, derived b value increased to a value of the right cheek and the results by lung ear to stimulate reflex point.

• The Journal of Internal Korean Medicine
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• v.15 no.1
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• pp.80-98
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• 1994

According to the original documents, Sagungsan is considered as an effective drug for controlling the hypertensive epistaxis induced by tension of autonomic nerve and it's hyperfunction. The present experiment was designed to understand the effect of Sagungsan extract on the hemostatic action, intracranial pressure, blood pressure and cardiovascular system in experimental animals. And thus the bleeding time, prothrombin time, capillary dilation, blood pressure, Intracranial pressure, and enzymatic analysis of the ATPase activities were studied. The result obtained here were as followings: 1. Sagungsan water extract reduced the bleeding time in mouse, and prolonged the prothrombin time in rabbits. 2. The drug extract increased the tail volume by capillary dilation in rats. 3. The drug extract inhibited the increase of intracranial pressure and arterial blood pressure in rabbits. 4. At the early time, the increase of arterial blood pressure by the drug extract significantly inhibited by pretreated atropin and regitine in rabbits. 5. The drug extract relaxed the smooth muscle by stimulating the Mg2+-Ca2+-ATPase activities of gastric sarcoplasmic reticulum isolated from rabbit stomach. 6. The drug extract stimulated the heart contraction by inhibiting the $Mg^{2+}-Ca^{2+}-ATPase$ activities of cardiac sarcoplasmic reticulum isolated from rabbit heart. The inhibitory mechanism was reversible and noncompatitive. 7. The drug extract increased the hepatic blood volume by stimulating the hepatic total ATPase activities and hepatic metabolism. 8. The drug extract acted as a tranquilizer by inhibiting the neural Na+-K+-ATPase activity. According to the results, Sagungsan water extract dilated the capillaries, stimulated the heart beat, and thus increased the blood flow with decreasing the intracranial pressure and blood pressure. These effects stanches the epistaxis collectively.

• Asian Journal of Turfgrass Science
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• v.2 no.1
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• pp.41-48
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• 1988

Research was conducted to obtain easy and secure methods for promoting germination of Korean lawngrass (Zoysia japonic a Steud.) seed. The effect of sodium hypochiorite (NaOCl) treatment on germination of seeds was compared with that of potassium hydroxide (KOH) treatment under conditions of duration of seed storage after harvest and temperature of treatment. Emergence of seedlings from soil and micro-flora infection rate among seed treatments were investigated. 1. The promotion of germination was much more evident in seeds treated with 4% NaOCl for 8 and 10 hours than in seeds treated with 25% or 30% KOH for 30 and 40 minutes. Longer durations of storage before seed treatment in KOH than in NaOCl were required to initiate enough germination. 2. Differences in temperature of treatment with NaOCT did not have much influence on germination of seeds and treatment temperature of 15˚C was better than that of 200˚C and 300˚C for promoting germination. But seed treatment with KOH significantly promoted germination with increasing temperature of treatment from I 5˚C to 20˚C and 30˚C. 3. GA$_3$ treatment enhanced germination in NaOCl-pretreated seeds at early stage of imbibition and in-creased about 10% germination after 10 days of imbibition in KOH-pretreated seeds. 4. NaOC1 treatments significantly decreased the rate of the infection of microflora in seed samples and enhaced emergence of seedlings from soil compared with KOH treatment. 5. NaOC1 treatment had advantage over KOH treatment with respect to ease of preparing, securing and handling in stimulating germinating of Korean lawngrass seeds.

• Korean Journal of Food Science and Technology
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• v.35 no.4
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• pp.702-707
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• 2003

This study was conducted to evaluate the effect of a growth-stimulating material (GSM) containing Eleutherococcus senticosuson on the longitudinal bone growth. The effects of GSM on proliferation zone and IGF-1 mRNA expression in rat growth plate, IGF-1 mRNA expression in MG-63 osteoblast and Hep-G2 hepatocyte, and bone growth of mouse tibia were studied. GSM significantly increased the proliferation zone in growth plate of proximal tibia (P<0.001) and the IGF-1 mRNA expression in growth plate was also increased (P<0.01). Treatment of GSM to MG-63 osteoblast and Hep-G2 hepatocyte also increased IGF-1 mRNA expression more than twice. In addition, bone mineral density of mouse tibia was significantly increased by GSM (P<0.05). Therefore, it was shown that GSM has an activity of bone growth promotion by increasing the expression of IGF-1, a major bone growth factor.

• Animal cells and systems
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• v.16 no.3
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• pp.207-214
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• 2012

Burns are one of the most devastating forms of trauma and wound healing is a complex and multicellular process, which is executed and regulated by signaling networks involving numerous growth factors, cytokines, and chemokines. Recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF) was specifically produced from rice cell culture through use of a recombinant technique in our laboratory. The effect of rhGM-CSF on promotion of deep second-degree burn wound healing on the back skin of a hamster model was evaluated through a randomized and double-blind trial. As macroscopic results, hamster skins of the experimental groups showed earlier recovery by new epidermis than the control groups. Immunohistochemical reactions of proliferating cell nuclear antigen and transforming growth factor-b1, which are indicators of cell proliferation, were more active in the experimental group, compared with the control group. On electron microscopy, basal cells in the epidermis of the experimental group showed oval nuclei, prominent nucleoli, numerous mitochondria and abundant free ribosomes. In addition, fibroblasts contained well-developed rough endoplasmic reticulum with dilated cisternae. Bundles of collagen fibrils filled the extracellular spaces. Particularly, ultrastructural features indicating active metabolism for regeneration of injured skin at 15 days after burn injury, including abundant euchromatin, plentiful free ribosomes, and numerous mitochondria, were observed. These findings suggest that use of rhGM-CSF could result in accelerated deep second-degree burn wound healing in animal models.