• 제목/요약/키워드: sterilization method

검색결과 275건 처리시간 0.03초

Improved Cleaning Method for Dental Instruments

  • Kim, In-Geol;Lee, Yun-Ji
    • Journal of Korean Dental Science
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    • 제3권2호
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    • pp.26-33
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    • 2010
  • We searched at the "PubMed.gov" and "jendodon.com" sites to conduct a literature review on dental instruments that are reused in clinical settings and on infection control involving pre-disinfection or sterilization cleaning/rinsing. The keyword "dental clean" was used for the Web search. We found the present official definition of instrument cleaning performed prior to disinfection or sterilization rather limiting ("removal of foreign matter (soil, organism, etc.) from the instruments"). Thus, we proposed to expand the definition to include the removal of oils applied to protect the metallic instruments and from corrosion, stains, and rust resulting from the frequent reuse of the instruments. Clinicians are found to clean their dental instruments (a) immediately after treating their patients or (b) following their treatment but not immediately afterward. In the latter case, we recommend presoaking to be added. Ultrasonic sterilization of 5~15 minutes is found to be more effective in terms of eliminating residual matter from the instruments compared to other methods. To check on the cleaning results, we recommend visual inspection, which can be quick and practical in clinical settings. The latest products being developed and marketed on the market address the related problems. Nonetheless, research must be continued on the effects of presoak, cleaning/rinsing, disinfection, and high-temperature or heating-based sterilization on the dental instruments and on dental clinicians' practices in cleaning, disinfection, and sterilization. We advise dental clinicians to select the proper cleaning methods and detergents for their instruments to help eliminate or prevent corrosion, staining, and rusting, to reduce the maintenance costs, and to ensure user-friendly instruments/apparatuses.

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환경전과정평가에 기반한 UV-LED 를 사용한 복합식 가습기에 관한 연구 (Study on Compound Humidifier Employing UV-LED using Environmental Life Cycle Assessment)

  • 최원식;박시현;이시왕;정영미;이화조
    • 한국정밀공학회지
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    • 제29권9호
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    • pp.931-937
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    • 2012
  • In this study, we assessed environmental impacts of compound humidifiers using environmental life cycle assessment and presented the ways to improvements in energy consumption of them. We found eco-design parameters and $CO_2$-eq emissions in each stage of raw material acquisition, manufacturing, transportation, use and disuse in life cycle of the compound humidifiers. The highest $CO_2$ emission is found to be in the stage of use among all stages of life cycle, which is mainly due to power consumption in thermal heating of heating coil for sterilization during humidification. The power consumption and $CO_2$ emission in the stage of use can be reduced to 1/4 and 1/3 at the highest estimate through improvement of sterilization method, respectively. We suggested the replacement of conventional thermal heating coil by ultra violet light-emitting diodes (UV-LED) for sterilization and then presented the experimental results on the sterilization effects of UV-LEDs.

통조림 식품의 살균중 에너지 소비 (Energy Consumption in Sterilization Process)

  • 이동선;신휴년;박노현;신동화;서기봉
    • 한국식품과학회지
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    • 제15권3호
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    • pp.282-286
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    • 1983
  • 통조림 살균에서 에너지의 효율적인 사용을 위하여 열전달형태, 살균온도, 가공공관, 살균방법등의 요인에 따른 에너지 소비를 검토한 바 고온(高溫)에서 소형관형으로 살균할 때가 적은 에너지가 소비되고 있으며, 이러한 차이는 대류형(對流型) 열전달식품(熱傳達食品)보다 전도형(傳導型) 열전달식품(熱傳達食品)의 살균(殺菌)에 현저하였다. 열수살균으로는 증기살균에 비해 전도형 식품의 살균에 적은 에너지를 소비하였으나, 대류형 식품에서는 효과적이지 못하였다. 본 실험에서의 여러 조건별 에너지 소비 data는 현장에서의 에너지 소비예측, 에너지 사용설계 등의 여러목적에 적용될 수 있는 것으로 판단된다.

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고온고압증기멸균이 우황청심원에 미치는 영향 (The Effects of High Temperature High Pressure Steam Sterilization on Woohwangchungsimwon)

  • 조창영;이인희;이재웅;김은지;이진호;김민정
    • 한방재활의학과학회지
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    • 제25권1호
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    • pp.45-52
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    • 2015
  • Objectives To check marker content for appropriate quality control of Woohwangchungsimwon sterilized to ensure microbiological safety and to observe antioxidant activity for any changes in efficacy. Methods To measure any effects of sterilization on the effective compounds, 8 ingredients of Woohwangchungsimwon were screened for any changes in marker content using HPLC-DAD. Using the colorimetric method on the microplate reader any changes in total phenolic compound and flavonoid levels were observed. Antioxidant activity was measured using the DPPH, ABTS, and FRAP. Results Of the ingredients of Woohwangchungsimwon, 8 were subject to quantitative analysis before and after sterilization. 21.6 mg and 1.93 mg of Glycyrrhizin was found in Glycyrrhiza uralensis Fischer pre and post sterilization, respectively. Decursin found in Angelica gigas Nakai increased from 0.16 mg to 0.29 mg after sterilization. Bilirubin found in Gallstone of Bostaurusvar. domesticus increased from 0.24 mg to 0.33 mg. Cinnamic acid found in Cinnamomum cassia Blume increased from 0.02 mg to 0.05 mg. Ginsenoside Rb1 found in Panax ginseng C. A. Meyer decreased from 0.02 mg to 0.14 mg. Paeoniflorin found in Paeonia lactiflora Pallas increased from 1.05 mg to 1.13 mg. Amygdalin found in Armeniacae Amarum Semen increased from 2.68 mg to 2.83 mg. L-muscone found in Musk increased from 0.63 mg to 0.76 mg. As for total phenolic compound and total flavonoid content, there was a 1.22 and 4.15-fold increase. DPPH and ABTS increased by 20.45% and 20.69%, respectively. FRAP activity was 2.78 times more active post stabilization. Conclusions This study confirmed that high temperature high pressure steam sterilization, a method used to ensure microbiological safety of Woohwangchungsimwon, does not affect marker content; in other words, does not affect quality of the Woohwangchungsimwon. It could also be seen that total phenolic compound and flavonoid content increased after sterilization. An antioxidant activity test showed that there was significantly increased activity of antioxidants.

대기압 유전체장벽방전을 적용한 플라즈마오존 공간살균장치에 관한 연구 (A study of the space sterilization device using atmospheric-pressure DBDs plasma)

  • 오희수;이강연;박주훈;정병호
    • 한국융합학회논문지
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    • 제13권3호
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    • pp.281-289
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    • 2022
  • 플라즈마오존은 높은 살균성능으로 인해 다양한 살균분야에서 활용되고 있다. DBD(유전체 장벽방전)에 사용되는 유전체는 주로 석영, 세라믹, 폴리머 등이 주로 사용된다. 유전체로 이루어진 레이어는 공급되는 전하의 양을 제한시키고 플라즈마가 유전체 면 위에서 고르게 발생할 수 있도록 하는 역할을 한다. 이러한 DBD를 이용한 플라즈마, 오존살균은 살균대상이나 주변 환경이 복잡한 구조로 된 경우가 많아 공간살균에 대한 개념을 수립하고 이에 대한 연구와 학문적 체계가 필요하다. 본 연구에서는 플라즈마라디칼과 오존 생성을 위해 대기압에서 DBD방식을 이용한다. 플라즈마오존의 발생을 위한 반응기의 구조는 세라믹 튜브 유전체와 스테인리스 도체를 일정한 간격으로 배치하여 유전체 장벽방전을 발생시키는 형태이다. 공간살균장치로서의 플라즈마오존 발생은 성능 면에서 우수한 살균장치로 인식되고 있으므로 장치의 설계와 검증을 통해 공간살균장치의 최적설계를 확립하고자 하며 제안된 방법을 기반으로 다양한 살균 어플리케이션을 개발하는데 기초를 제공한다.

느타리버섯 봉지 재배 시 수량증가를 위한 최적살균방법 (Optimum sterilization selection method for increasing yield of Pleurotus ostreatus on the bag­cultivation)

  • 이희덕;김용균;최현구
    • 한국자원식물학회지
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    • 제16권3호
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    • pp.218-222
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    • 2003
  • 느타리버섯 재배시 기존의 고압살균처리는 영세한 버섯재배 농민의 시설비나 운영면에서 비용이 많이 들어 야외 발효나 저온살균에 의한 저비용 고효율의 최적배지 선발을 위한 살균방법별 시험결과를 요약하면 가. 배지 재료에서는 톱밥+면실+왕겨 (1:1:1)배지와 톱밥+면실+비트 (5:3:2)처리가 비슷한 경향이었고 나. 살균방법 처리에서는 고압살균(121$^{\circ}C$, 90분) 방법보다 저온살균(50­6$0^{\circ}C$, 7일)처리가 균사배양 및 생육특성이 양호하였으며 다. 기존의 고압살균 톱밥+면실+비트(5:3:2)처리구 174g/1.5kg 대비 저온살균 톱밥+면실+왕겨 (1:1:1) 205g/1.5kg으로 18%증수되고 톱밥+면실+비트(5:3:2) 처리구에서는 207g/1.5kg으로 19% 증수되는 경향이었으나 톱밥+면실에 왕겨를 첨가하나 비트펄프를 첨가하나 유의성이 없었다. 라. 봉지 재배시 저온살균이 고압살균대비 경영비 12% 절감과 농가소득 향상에 기여 할 것으로 기대된다

미세수술적 난관복원술을 시행받은 36세 이상 환자에서의 임신율에 관한 연구 (Reproductive Outcomes after Microsurgical Reversal of Tubal Sterilization in Women 36 Years Age or Older)

  • 김석현;이규창;최수희;최영민;신창재;김정구;문신용;이진용;장윤석
    • Clinical and Experimental Reproductive Medicine
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    • 제25권3호
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    • pp.341-348
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    • 1998
  • Objective: To determine the reproductive outcomes of women undergoing microsurgical reversal of tubal sterilization at age 36 years or older. Materials and Methods: A series of 133 patients who received microsurgical reversal of the previously sterilized fallopian tubes at Seoul National University Hospital from July, 1980 to January, 1992 was reviewed and evaluated for clinical characteristics, pregnancy rates, and factors influencing the outcome of tubal reversal. Results: Of 133 patients, 78 (58.6%) had been sterilized by laparoscopic cautery. Loss of children was a leading cause for tubal reversal. The mean interval from tubal sterilization to tubal reversal was 65.0 months. The overall pregnancy rate was 52.6% (70/133), and the mean interval was 9.4 months from tubal reversal to pregnancy. Excluding 7 patients who were lost to follow-up, 76 pregnancies were confirmed in 63 patients with the delivery rate per patient of 66.7% (42/63). There were no significant differences in age, duration of tubal sterilization, postoperative tubal length between pregnant and non-pregnant groups. Conclusions: Microsurgical reversal of tubal sterilization could be a justifiable method in women 36 years age or older.

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비전리방사선을 이용한 미생물 균주 멸균효과 (Sterilization Effect of Microbial Strains by using Non-ionizing Radiation)

  • 정경환;서정민
    • 한국방사선학회논문지
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    • 제14권6호
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    • pp.833-839
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    • 2020
  • 전 세계적으로 중증 급성 호흡 증후군 코로나바이러스에 의해 감염예방 및 사회적 인식이 크게 변화되었으며, 그로 인해 의료 원내감염 노출이 높은 환자에 관한 감염관리 지침 및 과정을 더 강화하고 관리감시를 더 철저히 하고 있다. 감염예방을 위해서는 멸균이 최우선으로 시행이 되어야 하며, 저비용, 쉬운 설치와 조작이 쉬운 멸균법을 찾아 적절한 멸균효과를 제시하고자 한다. 본 연구에서는 충치균을 오염시켜 출력 4 W, 조사 시간 60, 150, 300 sec로 자외선 살균기를 이용하였으며, 레이저는 출력 0.8, 1.5 W로 각각 파장 266, 355 nm로 조사하였다. 자외선 살균기는 150 sec 이상에서 감염예방에 안전성을 나타내었으며, 레이저는 1.5 W와 0.8 W, 266 nm에서 예방의 안전성을 나타내었다. 결과에서 출력이 높고 253.7 nm에 가까운 파장일수록 멸균효과는 뛰어났다.

동결 건조한 한국인 상하악골에 대한 실험적 연구;I. 단순냉동 및 냉동 건조된 동종골의 멸균에 관한 실험적 연구 (AN EXPERIMENTAL STUDY ON THE FREEZE-DRIED BONE OF MAXILLA AND MANDIBLE IN KOREANS ;I. A microbiologic study of the sterility on the fresh-frozen and freeze-dried bone of human)

  • 엄인웅
    • Maxillofacial Plastic and Reconstructive Surgery
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    • 제13권4호
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    • pp.405-411
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    • 1991
  • To determine the sterility of the prepared allogeneic bone of the human, culture of the allografts prior to implantation was performed on fresh-frozen and freeze-dried bone. Before the use of allografts to the patients, it must be confirmed about the sterility, cellular cytotoxicity, immune reaction, and osteoinductive potential as a biomaterials. Oral and maxillofacial surgeons demand for allograft bone will be increased in the future. Wonkwang Bone Bank attempted to meet this demand, has performed series of experimental study on the allograft bone of the Koreans to evaluate the physical and chemical suitability of the bone since the surgeons applications will have broadened from benign cystic lesions to fracture malunions and non-unions, large segmental defects, and whole-bone allorgrafts after tumor surgery. The results obtained were as follows: 1. Freeze-drying(FD) only shoed some bactericidal effects of the normal and osteo bone but in cases of performing EO gas sterilization, the FD effects was not clear. 2. The fact that FD has little effect than the EO gas sterilization on normal bone postulated that the presence of microbiota may be due to an operation and bone processing procedure. 3. FD and EO gas sterilization had a remarkable effect on the osteo bone. 4. The sterilization effect were EO gas, Freeze-drying, Fresh-Frozen with descending order. But all sterilization method were not complete to preserve and implant allograft bone. We are now performing further continuous study on the radiation and chemical sterilization procedure to make safe and complete allograft bone.

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Minilaparotomy 불임술(不妊術)과 복강경불임술(腹腔鏡不妊術)에 관(關)한 비교연구(比較硏究) (A Comparison of Minilaparotomy and Laparoscopic Sterilization)

  • 배병주
    • Clinical and Experimental Reproductive Medicine
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    • 제4권1호
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    • pp.17-25
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    • 1977
  • Anderson(1937), Power and Barnes(1941) reported a study concerning a method of tubal sterilization in association with peritoneoscopy or laparoscopy in which they cauterized the tubes. There appears to have been a hiatus of interest in sterilization (cold or hot) associated with laparoscopy until reintroduction by Palmer(1963), Frangenheim(1964) and Steptoe(1967). On the other hand, for interval female sterilization, however, minilaparotomy is relatively new. By Saunder and Munsick(1972), John Lyle(1974), Frank Stubb(1974), Vitoon(1973) and B.C. Bai(1975), their own technique for interval female sterilization requires 2.0 to 2.5cm, incision at the margin of the mons pubis. In Korea, female sterilization by means of minilaparotomy firstly reported by B.C. Bai using Bai's uterine elevator, of his own device, early in 1975. Recently inteval female sterilization by laparoscopy and minilaparotomy are widely accepted throughout the world especially in Asian countries. Minilaparotomy is carried out from 1974, laparoscopic sterilization from 1976, and in this study each of 250 cases of those were analysed and discussed for the comparison at Seoul Red Cross Hospital. (1) In the age distribution, numerous clients were in their age of $31{\sim}35$ in laparoscopy as well as minilaparotomy. Average 33.7 years in L and 33.2 years in M. (M=minilaparotomy, L=laparoscopic sterilization) (2) As regarding living children, women having 3 children represented the greatest number, 113 cases out of 250 in M group and 102 cases out of 250 in L group. Average No. of child are 2.9 in Land 3.1 in M. (3) Concidering the operation day in the menstrml cycle, the greatest number of cases, those who underwent tubal sterilization during the days of $26{\sim}$, next during the $6{\sim}10$ days of the cycle in both group. (4) Concidering the operation time, 188 cases by laparoscopy were performed in $6{\sim}10$ minutes, 33 cases within 5 minutes and 24 cases in $11{\sim}15$ minutes. Maximum 50 minutes, minimum 4 minutes and average 8.3 minutes. The majority of cases (154 cases) by minilaparotomy required $6{\sim}10$ minutes and 67 cases $11{\sim}15$ minutes, 6 cases within 5 minutes. Maximum 30 minutes, minimum 4 minutes and average 10.4, minutes. In both groups, most of the reasons for the extra length were surgical difficulties such as thick abdominal wall, pelvic adhesion, less cooperation of patients in early period of this study. (5) Hospital stay after operation in L group required $3{\sim}4$ hours in 125 cases, $2{\sim}3$ hours in 41 cases, $4{\sim}5$ hours in 32 cases out of 250. Maximum 8 hours, minimum 1 hour and average 3.8 hours. In M group hospital stay required $6{\sim}7$ hours in 100 cases, over 7 hours in 85 cases, $5{\sim}6$ hours in 46 cases and so on. Maximum 14 hours, minimum 2 hours and average 6.5 hours. (6) The time between operation and gas passing in the majority cases of both groups, were $12{\sim}36$ hours. A veragetime 20.3 hours in L and 27.2 in M. (7) Laparoscopic sterilization coincident with induced abortion were carried out in 27 cases, laparoscopy with minilaparotomy to control for mesosalpingeal hemorrhage in 1 case. Minilaparotomy coincident with induced abortion were performed in 65 cases, D and C whit polypectomy, menstrual regulatian, and remaval of IUD in 1 case respectively. (8) In L group, 1 case of mesosalpingeal hemorrhage, 1 case of abdominal wall infection were complicated during operation. In M group, 1 case of uterine perfaration, 1 case of abdominal wall infection, 1 case of hemorrhage from omentum and 1 case of bloody vaginal discharge were complicated. No intensive medical treatment was required for those minor complications in both groups. (9) No failure has been recognized and these two sterilization techniques might be the simple, safe and the most effective method for permanent contraception at present time. There is no significant clinical defference between L and M group in this study.

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