• Title/Summary/Keyword: sterilization condition

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Comparison in Mycelial Growth and Fruit Body Development According to Sterilization Condition of Substrate in Pot Cultivation of Pleurotus ostreatus (느타리버섯 봉지재배시 배지 살균 조건에 따른 균사생장 및 자실체 생육 비교)

  • Lee, Yun-Hae;Cho, Yun-Jeong;Chi, Jeong-Hyun
    • The Korean Journal of Mycology
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    • v.30 no.2
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    • pp.99-103
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    • 2002
  • The suitable sterilization conditions in pot cultivation of varieties of Chunchunutari-2-ho and Suhannutari-1-ho Pleurotus ostreatus were investigated. The substrate formulations for the cultivation consisted of 40% poplar sawdust, 40% waste cotton and 20% beet pulp (PWB), and 40% poplar sawdust, 40% cotton seed hull and 20% beet pulp (PCB). The increase in the sterilization temperature caused decrease in the pH of the substrate. The moisture content of PWB substrate increased by 1.8%, but that of PCB substrate decreased by 4.1 % after low temperature sterilization ($12hr\;at\;60^{\circ}C$). However, a little change in the sterilization at higher temperature ($6hr\;at\;100^{\circ}C\;and\;90min\;at\;121^{\circ}C$) did not alter the moisture content of both substrate appreciably. The duration of mycelial growth was longest and its density was lowest in low temperature sterilization ($12hr\;at\;60^{\circ}C$). The high pressure sterilization ($90min\;at\;121^{\circ}C$) of PCB substrate produced high yield (236.6 g/pot) and the highest biological efficiency (96.6%) for Chunchunutari-2-ho. On the contrary the high temperatures sterilization ($6hr\;at\;100^{\circ}C$) of PCB substrate resulted in the yield of 259.1 g/pot with the biological efficiency 94.9% for Suhannutari-2-ho.

Sterilization and ecofriendly neutralization of seawater using electrolysis (전기분해에 의한 해수살균 및 친환경 중화에 관한 연구)

  • Yang, Jeong-Hyeon;Choi, Jong-Beom;Yun, Yong-Sup
    • Journal of Advanced Marine Engineering and Technology
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    • v.41 no.3
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    • pp.276-280
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    • 2017
  • In this study, we investigated the effect of sterilization and the neutralization of treated ballast water using seawater electrolysis. The electrolysis apparatus has a cation-selective membrane for passing the cation and a titanium electrode in each cell. We examined the sterilization effect after an incubation period of 24 hr. The oxidation reaction during electrolysis caused, the solution to become strongly acidic due to the generation of a hydroxyl group, and the oxidation reduction potentials(ORP) was increased to 800 - 1200mV. After the reduction reaction, the solution became alkaline(pH 9 - 12), and ORP was decreased to - 900 - - 750 mV. It might be possible to control the pH of ballast water through electrolysis. In addition, we demonstrated the effects of sterilization of ballast water containing generated hypochlorous acid using electrolysis under high ORP condition.

High Hydrostatic Pressure Sterilization of Putrefactive Bacteria in Salted and Fermented Shrimp with Different Salt Content (염농도가 다른 새우젓에 존재하는 유해가능 세균의 초고압 살균)

  • Mok, Chul-Kyoon;Song, Ki-Tae
    • Korean Journal of Food Science and Technology
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    • v.32 no.3
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    • pp.598-603
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    • 2000
  • This study was conducted to enhance the storage stability and the wholesomeness of salted and fermented shrimp manufactured with different salt levels by high hydrostatic pressure sterilization. The effects of high hydrostatic pressure treatment on the putrefactive bacteria in the fermented shrimps were investigated and the sterilization kinetics was analyzed. The initial microbial counts of the fermented shrimp with 8%, 18% salt aged for 6 weeks at $20^{circ}C$ were $1.6{\times}10^3,\;1.4{\times}10^4$ CFU/g for bacteria grown on Vibrio selective media, $9.3{\times}10^3,\;1.7{\times}10^5$ CFU/g for bacteria grown on Staphylococcus selective media, respectively, and null for bacteria grown on Salmonella selective media. The degree of the sterilization increased with the magnitude of the pressure and the treatment time. The fermented shrimp pressurized at 6,500 atm for 10 min had no detectable bacteria grown on Vibrio and Staphylococcus selective media at $10^2$ CFU/g detecting limit. High hydrostatic pressure sterilization could be analyzed by first order reaction kinetics. The $D_P$ values of the bacteria grown on Vibrio selective media of the fermented shrimp at 18% salt were higher than those at 8% salt, while those of the bacteria grown on Staphylococcus selective media showed an inverse trend. The $z_p$ values of 8% salt fermented shrimp were higher than those of 18% salt for both bacteria grown on Vibrio selective media and Staphylococcus selective media. High hydrostatic pressure treatment could be applied for the sterilization of the fermented shrimp, and the optimum high pressure sterilization condition was 10 min treatment at 6,500 atm.

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Effect of UV Sterilization on Quality of Centrifuged Takju during Storage (자외선 살균이 청징 탁주의 저장 중 품질에 미치는 효과)

  • Choi, Eun-Ju;Jung, Jin-Joo;Lee, Jang-Woon;Kang, Sung-Tae
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.3
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    • pp.461-466
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    • 2010
  • A cylindrical UV sterilization system was developed to decrease microorganisms in centrifuged Takju (CT). CT was run through 110 strips of honey comb type-teflon tubes and 9 UV lamps (1395 W) were equipped between teflon tubes. The optimum sterilization condition of CT was fixed for 1.5 min at 2 L/min in overall quality aspects; also, 5~6 log cycle decrease of viable cell numbers of total bacteria and yeast was observed at this operating condition. Quality changes of UV-sterilized CT were examined by UV irradiation of CT followed by storing at $30^{\circ}C$ for 8 days. To evaluate quality changes of UV-sterilized CT, pH, amino nitrogen content, acidity, reducing sugar content and viable cell numbers of total bacteria and yeast were measured. The growth of yeast and bacteria was retarded, showing around $10^8\;CFU/mL$ even after 4 days and $10^8\;CFU/mL$ after 6 days, respectively. Also, UV sterilized CT showed no changes in pH, titratable acidity, and amino nitrogen content during storage except reducing sugar content. UV sterilization did not cause significant difference in L, a, and b values between CT and UV-sterilized CT over the storage period.

Effect of Sterilization Conditions on Physicochemical and Sensory Properties of Three Korean Fried Rice Sauces (한식볶음밥 소스 3종의 살균조건에 따른 일반 품질분석 및 관능평가)

  • Hwang, Hyesung;Lim, Sujin;Shin, Kwangsun;Han, Jaeyoon;Lee, Sangbong;Shin, Youngjae
    • Journal of the East Asian Society of Dietary Life
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    • v.25 no.5
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    • pp.860-866
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    • 2015
  • Three types of Korean style fried rice sauce, 'Kimchi-tomato sauce', 'Doenjang butter sauce', and 'Seafood sauce' were developed in this study. Physicochemical properties such as pH, soluble solid content, acidity, salinity, and texture of sauces were analyzed after application of three different sterilization conditions ($110^{\circ}C/50min$, $115^{\circ}C/30min$, and $121^{\circ}C/20min$). However, no trend was observed in physicochemical properties of each sauce by sterilization conditions. The lightness (L-value) of doenjang butter sauce was higher than the other two sauces. Redness (a-value) and yellowness (b-value) of doenjang butter sauce and seafood sauce were greater than those of kimchi-tomato sauce due to different ingredients. Total viable cells were not detected under all sterilization conditions of the three types of sauce. The sensory evaluation results showed that $121^{\circ}C/20min$ of sterilization scored better than $110^{\circ}C/50min$ or $115^{\circ}C/30min$ sterilization in terms of overall preference of kimchi-tomato sauce and doenjang butter sauce. Overall, the preference of the three types of sauce showed that seafood sauce was best, followed by doenjang butter sauce and kimchi-tomato sauce.

Microbial Changes of Salted and Fermented Shrimp by High Hydrostatic Pressure Treatment (초고압처리에 의한 새우젓의 미생물변화)

  • Mok, Chul-Kyoon;Song, Ki-Tae;Lee, Sang-Ki;Park, Jong-Hyun;Woo, Gun-Jo;Lim, Sang-Bin
    • Korean Journal of Food Science and Technology
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    • v.32 no.2
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    • pp.349-355
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    • 2000
  • This study was conducted to enhance the storage stability of fermented shrimp with different salt contents using a high hydrostatic pressure. The effects of the magnitude of pressure and treatment time on the microorganisms of the fermented shrimp were investigated. The highest microbial counts with respect to the salt levels were observed at 18% salt, showing $3.4{\times}10^5\;CFU/g$ for general bacteria, $6.4{\times}10^4\;CFU/g$ for halophilic bacteria, $4.2{\times}10^5\;CFU/g$ for yeast and $3.0{\times}10^4\;CFU/g$ for halophilic yeast. The degree of sterilization increased with the magnitude of pressure and treatment time, and the sterilization could be analyzed by the first order reaction kinetics. The sterilization rate constants $(k_p)$ of the halophilic bacteria was lower than that of general bacteria. The $log(k_p)$ increased linearly with pressure and the slope of the regression line of the halophilic bacteria was greater than that of general bacteria, indicating that the sterilization of the halophilic bacteria was more dependent on the pressure. High hydrostatic pressure treatment was an effective non-thermal sterilization method for the salted and fermented shrimp, and the optimum treatment condition was for 10 min at 6,500 atm.

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Combination of Light Emitting Diode at 375 nm and Photo-reactive TiO2 Layer Prepared by Electrostatic Spraying for Sterilization

  • Hwang, Kyu-Seog;Jeon, Young-Sun;Choi, Tae-Il;Hwangbo, Seung
    • Journal of Electrical Engineering and Technology
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    • v.8 no.5
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    • pp.1169-1174
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    • 2013
  • The objective of this work was to increase the efficiency of ultraviolet-light emitting diodes at 375 nm for sterilization. Since $TiO_2$ had antibacterial properties, which were attributed to the appearance of hydroxyl radicals and superoxide radical anions on the surface species under ultra violet radiation at about 387 nm, photo-reactive layers such as Ag-doped $TiO_2$ were coated on aluminum substrates by electrostatic spraying. Crystallinity and surface morphology of the coating layer were examined by X-ray diffraction ${\theta}-2{\theta}$ scan and field emission-scanning electron microscope, respectively. In an antibacterial test, we observed above 99% reduction of Escherichia coli populations on 3 or 5 mol% Ag-doped $TiO_2$ layers after irradiation for 2 hrs at 375 nm, while very low inactivation on bare aluminum substrates occurred after irradiation as the same condition.

Effect of Acid Soaking and Thermal Sterilization on the Shape and Quality Characteristics of Tteokbokki Rice Cake (산침지와 가열 살균이 떡볶이 떡의 형상 변화와 품질 특성에 미치는 영향)

  • Jung, Hwabin;Yu, Chae Rin;Park, Hyeon Woo;Yoon, Won Byong
    • The Korean Journal of Food And Nutrition
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    • v.31 no.5
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    • pp.737-750
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    • 2018
  • Effects of acid soaking (AS) and thermal sterilization (TS) on the shape and quality characteristics of Tteokbokki rice cake (TRC) were investigated. The F-value of a sequential process (SP) of the combination of AS and TS was quantitatively determined with a reference microorganism of Bacillus cereus. F-values were evaluated according to the minimum and the maximum D-value of B. cereus and the reduction exponent (m=12). The heat penetration curves at cold point (CP) of TRC (400 g) were used to estimate the TS time at 121, 100, and $95^{\circ}C$. F-values of the SP were revised according to the adjusted m values after AS at different pH. The non-uniformity parameters (NUP) of TRC had no significant changes during AS but it dramatically increased after applying TS at a low pH by AS. The result of solubilized starch contents (SSC) demonstrated that the shape changes during SP are related to SSC. The texture characteristics and the whiteness were significantly influenced by a low pH condition (3.5) (p<0.05). Sensory analysis showed that a lower pH and a longer thermal processing time influenced negatively on the acceptability. This study showed that pH 4.0 and $95^{\circ}C$ was an optimum condition for the SP.

Processing and Characteristics of Canned Kwamaegi - 2. Processing and Characteristics of Canned Boiled Kwamaegi (과메기 통조림의 제조 및 특성 - 2. 보일드 과메기통조림의 제조 및 특성)

  • Park, Tae-Ho;Noe, Yu-Ni;Lee, In-Seok;Kwon, Soon-Jae;Yoon, Ho-Dong;Kong, Cheung-Sik;Oh, Kwang-Soo;Choi, Jong-Duck;Kim, Jeong-Gyun
    • Journal of Fisheries and Marine Sciences Education
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    • v.24 no.6
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    • pp.833-844
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    • 2012
  • This study was conducted to obtain basic data which can be applied to process of canned boiled Kwamaegi. Commercial Kwamaegi was cut into $2{\times}3$ cm lengths, filled 90 g into can (301-3) and added with 60 g water and then precooked for 10 min. at $100^{\circ}C$. And water layer was drained. The precooked Kwamaegi was packed into the can, and added with 60 g of mixed salt solution, which is mixed with salt 0.5% and bamboo salt 0.7%. The cans were seamed using a vacuum seamer, and then sterilized for various Fo values (Fo 8~12 min.) in a steam system retort at $121^{\circ}C$. pH, VBN, amino-N, total amino acid, free amino acid, color value (L, a, b), texture profile, TBA value, mineral, sensory evaluation and viable bacterial count of the canned boiled Kwamaegi produced with various sterilization condition(Fo 8~12 min.) were measured. There was no remarkable difference between sterilization conditions and sensual characteristics. The results showed that the product sterilized at Fo 8 min. was the most desirable because this condition is the most economical and tasty.

Preparation and Characterization of Canned Kwamaegi(I) - Preparation and Characterization of Canned Seasoned Kwamaegi - (과메기 통조림의 제조 및 특성(I) - 조미과메기 통조림의 제조 및 특성을 중심으로 -)

  • Yoon, Ho-Dong;Shim, Kil-Bo;Noh, Yu-Ni;Kong, Cheung-Sik;Nam, Dong-Bae;Park, Tae-Ho;Kim, Jeong-Gyun
    • Journal of Fisheries and Marine Sciences Education
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    • v.23 no.4
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    • pp.662-672
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    • 2011
  • This study was investigated to obtain basic data which can be applied to processing of canned seasoned kwamaegi. Commercial Kwamaegi was cut into $2{\times}3cm$ lengths, filled 90 g into can (301-3) and added with 60 g water before precooking for 10 min. at $100^{\circ}C$. The precooked Kwamaegi was packed into the can, and added with 60 g seasoning sauces, which was prepared by mixing soy sauce 23%, monosodium glutamate (MSG) 2%, sorbitol 2%, sesame oil 1%, vinegar 2%, starch syrup 17%, sake 5%, water 48%. The cans were sealed using a vacuum seamer and then sterilized for various Fo values (Fo 8~12 min.) in a steam system retort at $121^{\circ}C$. The factors such as pH, VBN, amino-N, total amino acid, free amino acid, color value (L, a, b), texture profile, TBA value, mineral, sensory evaluation and viable bacterial count of the canned seasoned Kwamaegi produced with various sterilization condition(Fo 8~12 min.) were measured. There was no remarkable difference between sterilization conditions and sensual characteristics. The results showed that the product sterilized at Fo 8 min. was the most desirable because this condition is most economical.