• Journal of Microbiology and Biotechnology
• /
• v.12 no.2
• /
• pp.301-305
• /
• 2002

The gene (ppaT) encoding Thermus caldophilus GK24 pyrophosphatase (Tca pyrophosphatase) was cloned and sequenced. The gene was found to contain an open reading frame encoding 175 amino acids with a calculated mass of 19,155 Da. The ppaT gene was expressed under the control of the tac promoter in Escherichia coli. The recombinant Tca pyrophosphatase was purified 21.4-fold with $56\%$ yield and specific activity of 25.7 U $mg^-1$, following a combination of heating (to denature the E. coli proteins) and one step of DEAE-Sephacel column chromatography. The native enzyme was found to have an approximate molecular mass of 110,000 Da and consisted of six subunits. The enzyme exhibited maximal activity at pH of 8.0-8.5 and was stable at $80-90^{\circ}C$. A divalent cation was absolutely required for the enzyme activity, with $Mg^2+$. being the most effective.

• Environmental Engineering Research
• /
• v.19 no.1
• /
• pp.83-89
• /
• 2014

The effects of initial solid concentration and microwave irradiation (MWI) time on the hydrolysis of waste activated sludge (WAS) were investigated. MWI time strongly influenced WAS hydrolysis for all initial solid concentrations of 8.20, 31.51, and 52.88 g VSS/L. For all WAS, the volatile suspended solids (VSS) solubilization degree ranged from 35.6% to 38.4% during a total MWI time of 10 min. Soluble chemical oxygen demand (SCOD) concentration increased at a rate proportional to the decrease of VSS during the MWI. However, the clearly different VSS solubilization patterns that were observed during the MWI were explained by the 2-step hydrolysis of WAS, consisting of the initial disintegration of the easily degradable part of the sludge, followed by the subsequent disintegration of the hardly degradable part of the sludge. WAS hydrolysis rates for 3 to 6 min of MWI were significantly lower than those for less than 3 min, or more than 6 min. From these results, 3 min MWI time and WAS of 31.51 g VSS/L (centrifugal thickener WAS) showed the most efficient hydrolysis of WAS at 36.0%. The profiles of total nitrogen (T-N) concentrations corresponded well to the SCOD increases in terms of the empirical formula of bacterial cell mass ($C_5H_7O_2N$). The negligible T-N increase and pH decrease during WAS hydrolysis by MWI will allow the application of this process to subsequent biological processes, such as anaerobic digestion.

• Journal of the Korean Solar Energy Society
• /
• v.31 no.3
• /
• pp.109-115
• /
• 2011

It can gain both the electric energy production and disperse of light at the same time if DSSC is applied in the building as window system. It means to help facade design and to be used in lighting, heating, cooling energy directly by applicating DSSC BIPV window that is possible to daylighting and materialization of color. For this, optical characteristics analysis that is basic step must take precedence. So, basic databases of DSSC are builded and optical performances according to the double and triple glazing are evaluated by analyzing spectral data of various colored DSSC. As a result, Green(4) has the highest visible transmittance that is 28.8%, and Blue(3) has the lowest that is 0.3%. And, in case of optical performance of Green(4) depending on the incidence angle, SHGC and Tsol are decreased sharply from more than $60^{\circ}C$. Finally, It is judged that Red(4), Green(1), (4), Blue(4) are suitable for application in office building because visible transmittance is high and solar heat gain coefficient is low relatively in spite of composing to double and triple glazing.

• 한국태양에너지학회:학술대회논문집
• /
• 2008.04a
• /
• pp.166-171
• /
• 2008

We cannot imagine any more the environment and energy problems are separated from our lives. The various attempts to solve these problems are made all over the world. In this study it was performed to analyze a different heating and cooling load depending on the earth-sheltering method and kind of soils by using TRNSYS 16 as the first step to establish the design guidelines for earth-sheltered architecture, one of the eco-friendly and low energy consuming building types. After performing this simulation, we found the result like this. It is the most lowest load in case of all of walls and roof being earth-sheltered. But considering of the load reduction rate, the effect of earth-sheltering the exterior vertical wall is more efficient for load reduction than the one of earth-sheltering a roof. And we got a lower thermal load in case of a lower heat conductivity of soil. Afterwards we will conduct a further study for boundary condition at earth-sheltered surface and the simulation analysis about the sensitivity variables. The final goal of this study is preparing the design guidelines for earth-sheltered architecture. so we will contribute to building energy saving.

• Journal of the Korean Ceramic Society
• /
• v.31 no.2
• /
• pp.129-136
• /
• 1994

Si3N4 with 6w/o Y2O3 and 1.5w/o Al2O3 has been gas pressure sintered and its densification behavior and the effect of the sintering variables on the microstructure and mechanical properties were investigated. Densification rate was higher at temperature below 1775$^{\circ}C$ and between 187$0^{\circ}C$ and 195$0^{\circ}C$ than between 1775$^{\circ}C$ and 187$0^{\circ}C$. The faster densification at temperature between 187$0^{\circ}C$ and 195$0^{\circ}C$ was thought to be due to the increased amount of liquid phase resulting from the increased amount of Si3N4 dissolving in the liquid. $\beta$-Si3N4 and Y-disilicate at temperatures below 1775$^{\circ}C$, and only $\beta$-Si3N4 at 187$0^{\circ}C$ and above were detected by XRD analysis. Three different two-step schedules were employed to obtain sintered body with above 99% theoretical density and to investigate the effect of the sintering variables on the density, the microstructure and the mechanical properties of the sintered body. The sintered density did not change with the heating rate, and the microstructure became coarser as the temperature increased. The strength decreased with the width of $\beta$-Si3N4 grain, while the fracture toughness increased with the square root of it. A ceramic cutting tool made of the sintered body showed an uniform flank wear after the cutting test.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.20 no.11
• /
• pp.3538-3548
• /
• 1996

In this study, as a part of basic study for developing the simulation program for the assemssment of reliability of electronic EMC packaging which covers from EMC mixing step to thermal analysis, comparison between a measured and predicted values of material properties of EMC and finitde element analysis of thermal stress are performed. For the experimental testing specimens of fifty, sixty hive and eighty percent filler($13\mu m$, spherical silica) weight fraction are fabricated using tranfer molding. Coefficient of thermal expansion, elastic modulus and thermla conductivity are measured using these specimens and then these measured values are compared with the predicted values by various equations ( such as dilute suspension method. self consistent method, generalized self consistent method, Hashin-Shtrikman's bounds. Shapery's bounds, Nielsen's method and others). Measured values are distributed within the upper and lower bounds of equations. Measured elastic modulus and coefficient of thermal expansion approaches closely the perdicted values with self consisten mehtod and upper bound of Shaperys equation respectively. However small differences of thermal conductivity between the different filler volume fraction are obserbed. FEM analysis indicates that firstly stress is concentrated at the corner section of EMC and secondly EMC with eighty percent filler weight fraction shows less thermal stress when package is cooling down and relatively high thermal stress when package is heating up.

• Journal of Advanced Marine Engineering and Technology
• /
• v.36 no.4
• /
• pp.430-436
• /
• 2012

This study was conducted to measure battery's voltage drop in a compact electric vehicle to get driving performance in various driving situations. In the experiment, to evaluate the energy consumption and milage, system performance have measured with changing of the driving speed and the reduction of driving distance when the heater was operating. The battery of the car in this study is lead type storage battery. The driving velocity was changed from 10km/h to 50 km/h with 20km/h intervals and the operating step of the heating device. As results, the electronic consumption rate was maximum at 35 km/h of vehicle speed and if the driver turning the heater at maximum, capacity will lead to 35% of energy consumption increment.

• Bulletin of the Korean Chemical Society
• /
• v.35 no.7
• /
• pp.1985-1988
• /
• 2014

$Cu_2ZnSnSe_4$ (CZTSe) thin films were synthesized on transparent conducting oxide glass substrates via a simple, non-toxic, and low-cost process using a precursor solution paste. A three-step heating process (oxidation, sulfurization, and selenization) was employed to synthesize a CZTSe thin film as an absorber layer for use in thin-film solar cells. In particular, we focused on the effects of sulfurization conditions on CZTSe film formation. We found that sulfurization at $400^{\circ}C$ involves the formation of secondary phases such as $CuSe_2$ and $Cu_2SnSe_3$, but they gradually disappeared when the temperature was increased. The formed CZTSe thin films showed homogenous and good crystallinity with grain sizes of approximately 600 nm. A solar cell device was tentatively fabricated and showed a power conversion efficiency of 2.2% on an active area of 0.44 $cm^2$ with an open circuit voltage of 365 mV, a short current density of 20.6 $mA/cm^2$, and a fill factor of 28.7%.

• 한국지구물리탐사학회:학술대회논문집
• /
• 2003.11a
• /
• pp.470-475
• /
• 2003

KIGAM (Korea Institute of Geoscience and Mineral Resources) launched a new project to develop the low-enthalpy geothermal water in the area showing high geothermal anomaly, north of Pohang city, for large-scale space heating from KORP (Korea Research Council of Public Science & Technology) funding. Surface geologic and geophysical surveys including Landsat TM image analysis, gravity, magnetic, Magnetotelluric (MT) and controlled-source audio-frequency MT (CSAMT) and self-potential (SP) methods have been conducted and the possible fracture zone was found that would serve as deeply connected geothermal water conduit. By the end of 2003, two test wells of 1 km depth will be drilled and various kinds of borehole survey along with additional MT measurements and sample analysis will follow and then the detailed subsurface condition is to be characterized. Next step would be drilling the production well of 2 km depth and all further steps remain to be determined depending upon the results of the test well studies.

• Transactions of Materials Processing
• /
• v.6 no.3
• /
• pp.239-249
• /
• 1997

The behaviour of alloys in the semi-solid state strongly depends on the imposed stress state and on the morphology of the phase which can vary from dendritic to globular. To optimal net shape forging of semi-solid materials, it is important to investigate for filling phenomena in forging process of arbitrarily shaped dies. To produce a automotive part which has good mechanical property, the filling pattern according to die velocity and solid fraction distribution has to be estimated for arbitrarily shaped dies. Therefore, the estimation of filling characteristic in the forging simulation with arbitrarily shaped dies of semi-solid materials are calculated by finite element method with proposed algorithm. The proposed theoretical model and a various boundary conditions for arbitrarily shaped dies is investigated with the coupling calculation between the liquid phase flow and the solid phase deformation. The simulation process with arbitrarily shaped dies is performed to the isothermal conditions of two dimensional problems. To analysis of forging process by using semi-solid materials, a new stress-strain relationship is described, and forging analysis is performed by viscoelastic model for the solid phase and the Darcy's law for the liquid flow. The calculated results for forging force and filling limitations will be compared to experimental data. The filling simulation of simple products performed with the uniform billet temperature(584$^{\circ}C$) from the induction heating by the commercial package MAGMAsoft. The initial step of computation is the touching of semi-solid material with the end of die gate and the initial concept of proposed system just fit with the capability of MAGMAsoft.