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Antimicrobial Activity Screening of Sixty-four Evergreen Woody Species According to Extraction Conditions against Trichophyton mentagrophytes (상록성 목본 64종의 추출조건에 따른 무좀원인균의 항균활성 스크리닝)

  • Jang, Bo Kook;Chi, Lai Won;Cho, Ju Sung;Lee, Cheol Hee
    • Korean Journal of Plant Resources
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    • v.31 no.4
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    • pp.330-341
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    • 2018
  • This study was performed to investigate and measure the antimicrobial activity of evergreen woody species extracts on Trichophyton mentagrophytes. To do this, leaves and stems were collected from Wando and Jeju islands, and were used for the extraction with different solvents (i.e., distilled water, 80% ethanol, and 100% methanol), and at different ultrasonic extracting times (i.e., 15, 30, and 45 minutes). The experiment was conducted by using the agar diffusion method. The clear zone was measured after incubating the paper disc containing the plant extract in a bacterial culture medium. The controls were synthetic antimicrobials, methylparaben and phenoxyethanol, at concentrations of 0.4, 1, 2, and 4 mg/disc. Altogether, extracts of 56 out of 64 species used in this study had inhibitory activity, which confirmed their antimicrobial activity against Athlete's foot. Among them, the crude ethanolic extract of Elaeocarpus sylvestris in 45 min showed a zone of inhibition < 20.2 mm, while the clear zone of Actinodaphne lancifolia ethanolic extraction for 30 min was 23.5 mm. Also, Quercus acuta, Dendropanax morbiferus and Daphne odora showed clear zones of 28.0 mm (45 minutes ethanolic extraction), 20.5 mm (45 minutes crude methanolic extraction) and 19.7 mm (45 minutes methanolic extraction), respectively. Thus, these results confirm that the extracts of evergreen woody species have therapeutic potential against Athlete's foot, and suggest that in order to extract adequate amounts of antimicrobial substance from the plant sources, ideal extraction condition has to be considered.

Comparison of Yield and Workload depending on Stem Training Methods in Oriental Melon Hydroponics (참외 수경재배에서 줄기 유인 방법에 따른 수확량 및 작업 강도 비교)

  • Lee, Dong Soo;Kwon, Jin Kyung;Yun, Sung Wook;Lee, Si Young;Seo, Min Tae;Lee, Hee Ju;Lee, Sang Gyu;Kang, Tae Gyoung
    • Journal of Bio-Environment Control
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    • v.30 no.4
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    • pp.377-382
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    • 2021
  • Oriental melon (Cucumis melo L.) is generally cultivated on the ground by creeping culture. A farmer has a higher workload for training stems. This study was conducted to find out a new cultivation of oriental melon to reduce a workload and improve the quality of fruit. There were three treatments for training stem of oriental melon; upward stem growing, downward stem growing, control (creeping stem growing). The results of the plant growth and the net photosynthesis showed higher in upward stem growing. The root activity was higher in downward stem attract. The yield was not significant as 4,055kg/10a in upward stem attract and 3,983kg/10a in downward stem attract. According to the results of the ergonomic agricultural workload evaluation, in the case of the working posture, the working posture of creeping cultivation methods (squatting, bending) showed a higher risk level than the upward and downward cultivation methods. Therefore, it is recommended the upward stem attract of oriental melon is a new cultivation as well as an alternative method for creeping stem attract in terms of improving the plant growth and yield, and reducing the workload.

Taxonomic identity of Crepidiastrum ×nakaii recorded on Hongdo Island (홍도고들빼기의 분류학적 실체)

  • JANG, Young-Jong;CHOI, Byoung-Hee
    • Korean Journal of Plant Taxonomy
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    • v.51 no.3
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    • pp.198-204
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    • 2021
  • The plant known as "Hong-do-go-deul-ppae-gi" on Hongdo Island, belonging to the genus Crepidiastrum, has been recorded as C. ×nakaii, a hybrid between C. denticulatum and C. platyphyllum. During a survey of the flora of Hongdo Island, we reexamined the taxonomic identity of "Hong-do-go-deul-ppae-gi". The morphological traits, geographical distribution, and internal transcribed spacer (ITS) sequences of "Hong-do-go-deul-ppae-gi" were compared to related species within the genus. A morphological examination revealed that the plant was similar to C. lanceolatum in that it has subshrub stems with caudex stout, 8 inner involucrals bracts, and 10 to 12 florets. In contrast, similarities to C. denticulatum were observed in terms of radical leaves deciduous in the flowering period, cauline leaves membranous with acute serrations, and beaked achenes. Furthermore, C. denticulatum and C. lanceolatum grew on Hongdo Island and nearby areas, but C. platyphyllum was not distributed in these areas. The results of morphological and distributional investigations revealed that the Hongdo Island plant should be regarded as C. ×muratagenii, a hybrid between C. denticulatum and C. lanceolatum, not C. ×nakaii, which is a hybrid between C. denticulatum and C. platyphyllum. However, the resolution of the ITS sequences was insufficient such that we could not separate the examined species.

Current Status and Prospects for the Hemp Bioindustry (대마 생물산업의 현황과 전망)

  • Sohn, Ho-Yong;Kim, Mun-Nyeon;Kim, Young-Min
    • Journal of Life Science
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    • v.31 no.7
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    • pp.677-685
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    • 2021
  • Cannabis sativa L. belongs to the Cannabaceae family and is an annual herbaceous flowing plant. The plants can be classified into narcotic marijuana and nonnarcotic hemp. Different parts of C. sativa L. have been used as food, medicine, cosmetics, fiber and textile. However, the use of leaf, flower, and seed of C. sativa L was forbidden in Korea in January 1977 as a result of the Cannabis Control Act due to the narcotic properties. The plant's mature stems have limited uses for the production of fiber and sheets. Recently, various cannabinoids, terpenes and essential fatty acids were identified from C. sativa L., and their safety and useful bio-activities, such as neuroprotective, anti-inflammation, antithrombosis, antiepileptic, and antimicrobial activities, and the relief of pain, have been highlighted. Furthermore, the process of reduction of tetrahydrocannabinol, a representative narcotic compound, and the isolation of cannabidiol, a nonnarcotic active compound in C. sativa L., have been determined. These findings resulted in the legalization of C. sativa L. in Korea for medical use in December 2018 and the exclusion of C. sativa L. from the narcotic list of the UN Commission on Narcotics Drugs (UNCND) in December 2020. Therefore, developments of various high-value added products have commenced worldwide. Additionally, in 2021, the Korean government deregulated special zones based on hemp. In this study, the current status and the prospect of the hemp industry, as well as essential techniques for developing new hemp products, are provided for the activation of the Korea Green-Rush.

Breeding of a Red Rose Cultivar, 'Noble Red' with Good Color and Shape (화형과 화색이 우수한 적색 스탠다드 장미 '노블레드' 육성)

  • Kim, Won-Hee;Kim, Seung-Tae;Lee, Eun-Kyung;Lee, Su-Young
    • Korean Journal of Breeding Science
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    • v.42 no.3
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    • pp.280-283
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    • 2010
  • A new standard rose cultivar 'Noble Red' was bred by the cross between the pink standard cultivar 'Silba 87' and the red standard cultivar 'Bravo' at the National Institute of Horticultural and Herbal Science. The cross was made in 1999 and 'Noble Red' was finally selected in 2003 after investigating the characteristics such as flower Color, Fragrance, stem length, stem diameter, weight, and number of leaves for three years from 2001 to 2003. 'Noble Red', a red standard cultivar grows vigorously and has good flower shape. The major characteristics of this cultivar are $125stems/m^2/year$ in yield, 71.9 cm in length of cut flower, 6.0cm in flower diameter, 30.8 in petal number, and 9.2 days in vase life. This cultivar can be propagated by both cutting and grafting. The consumer's preference of this cultivar is relatively higher than that of control cultivar, 'Rote Rose'.

Breeding of a White Rose Cultivar, 'Snow Ball' with Good Color and Good Vase Life (절화수명이 긴 백색 스탠다드 장미 '스노우 볼' 육성)

  • Kim, Won-Hee;Kim, Seung-Tae;Lee, Eun-Kyung;Lee, Su-young
    • Korean Journal of Breeding Science
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    • v.42 no.3
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    • pp.276-279
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    • 2010
  • A new standard rose cultivar 'Snow Ball' was bred by the cross between the red standard cultivar 'Red Sandra' and the scarlet standard cultivar 'Baroness' at the National Institute of Horticultural and Herbal Science, in Suwon. The cross was made in 1998 and 'Snow Ball' was finally selected in 2003 after investigating the characteristics for three years from 2001 to 2003. 'Snow Ball', a white standard cultivar grows vigorously and has good flower shape. The major characteristics of this cultivar are $90.3stems/m^2/year$ in yield, 57.8 cm in length of cut flower, 6.1 cm in flower diameter, 85.7 in petal number, and 15.0 days in vase life. This cultivar can be propagated by both cutting and grafting. The consumer's preference of this cultivar is relatively higher than that of control cultivar, 'Tineke'.

A Dark Pink Freesia 'Happy Birthday' with Semi-Double Multi-flowering (진분홍색 반겹꽃 다화성 프리지아 신품종 '해피버스데이' 육성)

  • Cho, Hae-Ryong;Rhee, Hye-Kyung;Kim, Mi-Seon;Park, Sang-Kun;Shin, Hak-Ki;Joung, Hyang-Young;Lim, Jin-Hee
    • Korean Journal of Breeding Science
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    • v.43 no.3
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    • pp.210-213
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    • 2011
  • A dark pink semi-double freesia (Freesia hybrida Hort.) 'Happy Birthday' was bred from a cross between 'Pink Jewel', F1 progeny of 'Michelle' and 'Rossini' as a female and 'White Lace' as a male parent. Test for the growth vigor and flowering characteristic were conducted from 2002 to 2007. A new variety 'Happy Birthday' has semi-double flower type with dark pink color (RHS color chart #RP66C). It has multiple flowers, distinctive flower shape, upright leaves and also stalks. The average yield of this variety 'Happy Birthday' counted over 6.7 stems per plant per season in the standard greenhouse cultivation method. It has also good production of cormlets, vigorous growth. A new variety 'Happy Birthday' is suitable for the greenhouse conditions during winter season in Korea.

Analysis of Bacterial Wilt Symptoms using Micro Sap Flow Sensor in Tomatoes (식물 생체정보 센서를 활용한 토마토 풋마름병 증상 분석)

  • Ahn, Young Eun;Hong, Kue Hyon;Lee, Kwan Ho;Woo, Young Hoe;Cho, Myeong Cheoul;Lee, Jun Gu;Hwang, Indeok;Ahn, Yul Kyun
    • Journal of Bio-Environment Control
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    • v.28 no.3
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    • pp.212-217
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    • 2019
  • Bacterial wilt caused by Ralstonia solanacearum is a major disease that affects tomato plants widely. R. solanacearum is a soil born pathogen which limits the disease control measures. Therefore, breeding of resistant tomato variety to this disease is important. To identify the susceptible variety, degree of disease resistance has to be determined. In this study, micro sap flow sensor is used for accurate prediction of resistant degree. The sensor is designed to measure sap flow and water use in stems of plants. Using this sensor, the susceptibility to bacterial wilt disease can be identified two to three days prior to the onsite of symptoms after innoculation of R. solanacearum. Thus, this find of diagnosis approach can be utilized for the early detection of bacterial wilt disease.

Effects of Different Sowing Methods on Growth and Yield of Proso Millet (Panicum miliaceum L.) and Foxtail Millet (Setaria italica L.) (파종방법에 따른 기장과 조의 생육 및 수량)

  • Jung, Ki-Youl;Choi, Young-Dae;Chun, Hyen-Chung;Lee, Sang-Hun;Jeon, Seung-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.63 no.4
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    • pp.384-389
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    • 2018
  • Cultivation techniques of proso millet (Panicum miliaceum L.) and foxtail millet (Setaria italica L.), such as sowing, weeding, and harvesting, are often not properly utilized, resulting in low productivity rates. The goal of this study was to develop cultivation techniques to increase millet productivity rates and reduce labor inputs. We evaluated the effects of different machine sowing methods compared with manual sowing methods from 2014 to 2016. The three machine sowing methods were dibbling, drilling, and broadcasting. Compared with manual sowing, the broadcasting method decreased labor times by 16.07 and $14.23hr10a^{-1}$ for proso and foxtail millets, respectively. Proso millet showed greater culm lengths at 138 and 125 cm with the broadcasting method for two years, whereas foxtail millet after three years had greater culm lengths from both the drilling and broadcasting methods. Both crops exhibited the thickest culm stems in the $3^{rd}$ year. The greatest number of seeds of the panicle were from the manual sowing method in both crops. Compared with manual sowing, broadcasting increased grain yields by 15-35% and 9.1-28% for proso and foxtail millets, respectively. Therefore, we suggest that the application of broadcasting in mechanical sowing will increase labor efficiency and yields of proso and foxtail millets.

First Report of a Bracken Blight Disease Caused by Didymella sp. (Didymella sp.에 의한 고사리 신규 마름병 발생 보고)

  • Lee, Jung Eun;Kim, Ki Beom;Park, Ju Eun;Kim, Da-Woon;Shin, Yoo-Kyoung;Yun, Sung-Hwan;Chung, Young-Ryun
    • Research in Plant Disease
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    • v.25 no.3
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    • pp.143-148
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    • 2019
  • An outbreak of new disease with leaf and stem blight symptom occurred at bracken-growing fields in Namhae-gun, Gyeongsangnam-do, Korea during the last 4 years. This new disease caused significant yield losses on bracken production in this area. We have collected diseased leaves and stems showing the blight symptom in May, July, and October 2018 to investigate causal pathogens. A total of 92 fungal isolates were obtained from the diseased samples and their pathogenicity was tested on healthy bracken leaves. From the total isolates, 22 isolates were able to produce the leaf blight symptom similar to the original one found in the fields. To identify two fungal pathogens which showed higher virulence levels compared to other pathogenic isolates, we constructed phylogenetic trees using the nucleotide sequences of genes for ribosomal RNA, RNA polymerase beta subunit, beta tubulin, and internal transcribed region. Most phylogenetic trees constructed indicate that both isolates, which are identical to each other, reside in a clade of the genus Didymella and possibly similar to D. rumicicola or D. acetosellae. Nevertheless, the exact identification of these pathogens at the species level needs further investigations. This is the first report of a blight disease on bracken by Didymella sp.