• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2015년도 학술발표회
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• pp.180-180
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• 2015

Weather radar has been widely used in measuring precipitation and discharge and predicting flood risks. The radar rainfall estimate has one of the essential problems in terms of uncertainty and accuracy. Previous study analyzed radar errors to reduce its uncertainty or to improve its accuracy. Furthermore, a recent analyzed the effect of radar error on rainfall-runoff using spatial error model (SEM). SEM appropriately reproduced radar error including spatial correlation. Since the SEM does not take the time dependence into account, its time variability was not properly investigated. Therefore, in the current study, we extend the SEM including time dependence as well as spatial dependence, named after Spatial-Temporal Error Model (STEM). Radar rainfall events generated with STEM were tested so that the peak runoff from the response of a basin could be investigated according to dependent error. The Nam River basin, South Korea, was employed to illustrate the effects of STEM on runoff peak flow.

• Journal of Veterinary Science
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• 제23권6호
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• pp.89.1-89.7
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• 2022

It is challenging to diagnose metastatic tumors whose cellular morphology is different from the primary. We characterized canine primary pulmonary adenocarcinoma (PAC) and its xenografted tumors by histological and immunohistochemical analyses for critical diagnostic and cancer stem cell (CSC) markers. To generate a tumor xenograft model, we subsequently transplanted the tissue pieces from the PAC into athymic nude mice. Immunohistochemical examination was performed for diagnostic (TTF-1, Napsin A, and SP-A) and CSC markers (CD44 and CD133). The use of CSC markers together with diagnostic markers can improve the detection and diagnosis of canine primary and metastatic adenocarcinomas.

• 한국발생생물학회지:발생과생식
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• 제27권2호
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• pp.57-65
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• 2023

Kidney disease affects a significant portion of the global population, yet effective therapies are lacking despite advancements in identifying genetic causes. This limitation can be attributed to the absence of adequate in vitro models that accurately mimic human kidney disease, hindering targeted therapeutic development. However, the emergence of human induced pluripotent stem cells (PSCs) and the development of organoids using them have opened up a way to model kidney development and disease in humans, as well as validate the effects of new drugs. To fully leverage their capabilities in these fields, it is crucial for kidney organoids to closely resemble the structure and functionality of adult human kidneys. In this review, we aim to discuss the potential of using human PSCs or adult kidney stem cell-derived kidney organoids to model genetic kidney disease and renal cancer.

• BMB Reports
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• 제57권8호
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• pp.352-362
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• 2024

Hematopoietic stem cell transplantation (HSCT) remains an indispensable therapeutic strategy for various hematological diseases. This review discusses the pivotal role of bone marrow (BM) niches in influencing the efficacy of HSCT and evaluates the current animal models, emphasizing their limitations and the need for alternative models. Traditional animal models, mainly murine xenograft, have provided significant insights, but due to species-specific differences, are often constrained from accurately mimicking human physiological responses. These limitations highlight the importance of developing alternative models that can more realistically replicate human hematopoiesis. Emerging models that include BM organoids and BM-on-a-chip microfluidic systems promise enhanced understanding of HSCT dynamics. These models aim to provide more accurate simulations of the human BM microenvironment, potentially leading to improved preclinical assessments and therapeutic outcomes. This review highlights the complexities of the BM niche, discusses the limitations of current models, and suggests directions for future research using advanced model systems.

• 한국작물학회지
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• 제54권1호
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• pp.29-35
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• 2009

The aim of the present study was to update the calibration that is used for the measurement of the total nitrogen content in the rice plant samples by using the visible and near infrared spectrum. Before the equation merge, correlation coefficient of calibration equation for nitrogen content on each rice parts was 0.945 (Leaf), 0.928 (Stem), and 0.864 (Whole plant), respectively. In the calibration models created by each part in the rice plant under the various regression method, the calibration model for the leaf was recorded with relatively high accuracy. Among of those, the calibration equation developed by Partial least squares (PLS) method was more accurate than the Multiple linear regression (MLR) method. The calibration equation was sensitive based on variety and location variations. However, we have merged and enlarged various of the samples that made not only to measure the nitrogen content more accurately, but also later sampling populations became more diversified. After merging, $R^2$ value becomes more accurate and significantly to 0.950 (L.), 0.974 (S.), 0.940 (W.). Also, after removal of outlier, R2 values increased into 0.998, 0.995, and 0.997. In view of the results so far achieved, Standard error of prediction (SEP) and SEP (C) were reduced in the stem and whole plant. Biases were reduced in the leaf, stem as well as whole plant. Slopes were high in the stem. Standard deviation reduced in the stem but $R^2$ was high in the stem and whole plant. Result was indicated that calibration equation make update, and updating robust calibration equation from merge function and multi-variate calibration.

• 한국환경과학회지
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• 제33권7호
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• pp.453-462
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• 2024

This study was carried out for the purpose of selecting the most appropriate taper equation for the actual stands of Pinus thunbergii in the southern coastal region of Korea and then developing a stem volume table to provide basic data for rational management. To develop a volume table of Pinus thunbergii in this region of Korea, 59 sample trees with various diameter distributions were selected and stem analysis was performed. As a result of stem analysis, two trees with abnormal diameter and height growth as the age increased were rejected, and 57 trees were analyzed. To develop the taper equation, seven major variable exponential equations were used, including Kozak 1988, 1994, 2001, 2002, Bi 2000, Muhairwe 1999, and Sharma and Parton 2009. As a result of parameter estimation and statistical verification, the Kozak 1988 model showed the highest goodness of fit with Fit I (Fit Index), RMSE 1.5620, Bias 0.0031, and MAD 1.0784. The diameter of each 10cm stem ridge for the selected model was estimated, and a stem volume table was produced using the mensuration of division (end area formula) using the Smalian equation. As a result of two-sample T-test for volume table of this study and current yield table, the volume for this study was found to be significantly larger at all observation points (p < 0.001). Even for the same tree species, it is judged that differentiated volume tables are needed for each growth environment characteristic.

• 생물환경조절학회지
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• 제8권4호
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• pp.232-241
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• 1999

본 논문에서는 온실재배 토마토에서 수분 상태를 고려한 정밀한 관개를 위하여 경직경의 변화에 근거한 자동관개 시스템을 구현하고자 하였으며 이와 같은 작물적 지표에 의한 관개제어시스템을 포트재배(closed feeding system) 조건과 포장재배 조건의 재배에 적용하여 토마토의 수량과 과실의 품질에 대하여 그 적합도를 평가하였다. 작물적 지표에 의한 관개 자동화를 위하여 경직경 측정 센서를 이용하여 측정한 경직경 일증가량(DI)를 관개 개시점의 결정에 이용하고, 동시에 이때의 관개량을 결정하기 위하여 토마토 증산모델을 적용한 마이크로 컴퓨터 자동관개 시스템을 구현하고 그 성능을 평가하였는데, 이 자동 관개 시스템을 이용하여 관개처리한 결과 포트재배에서는 토양수분에 의한 관개방식보다 수량 및 과실의 당도가 증가하였으나 포장조건에서는 관개방법간에 큰 차이가 없었다. 따라서 경직경 증가에 근거한 관개 개시점을 판단하고 관계량을 결정하는 증산모델을 사용하는 컴퓨터 시스템은 특히 closed feeding System에서 유용하며 토양조건에서는 토양의 상태, 작물의 반응 등을 고려하여 적용하여야 할 것으로 판단되었다.

• BMB Reports
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• 제48권12호
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• pp.645-646
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• 2015

The sympathetic nervous system (SNS) or neurotransmitters in the bone marrow microenvironment has been known to regulate hematopoietic stem cell (HSC) functions such as self-renewal, proliferation and differentiation. However, the specific role of neuropeptide Y (NPY) in this process remains relatively unexplored. In this study, we demonstrated that NPY deficient mice have significantly reduced HSC numbers and impaired bone marrow regeneration due to apoptotic destruction of SNS fibers and/or endothelial cells. Moreover, NPY treatment prevented bone marrow impairments in a mouse model of chemotherapy-induced SNS injury, while conditional knockout mice lacking the Y1 receptor in macrophages did not restore bone marrow dysfunction in spite of NPY injection. Transforming growth factor-beta (TGF-β) secreted by NPY-mediated Y1 receptor stimulation in macrophages plays a key role in neuroprotection and HSC survival in the bone marrow. Therefore, this study reveals a new role of NPY in bone marrow HSC microenvironment, and provides an insight into the therapeutic application of this neuropeptide.

• BMB Reports
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• 제39권4호
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• pp.426-431
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• 2006

Embryonic stem cells (ESCs), representing a population of undifferentiated pluripotent cells with both self-renewal and multilineage differentiation characteristics, are capable of spontaneous differentiation into cardiomyocytes. The present study sought to define the kinetic characterization of lactate dehydrogenase (LDH) and creatine kinase (CK) of ESC- and neonatal-derived cardiomyocytes. Spontaneously differentiated cardiomyocytes from embryoid bodies (EBs) derived from mouse ESC line (Royan B1) and neonatal cardiomyocytes were dispersed in a buffer solution. Enzymes were extracted by sonication and centrifugation for kinetic evaluation of LDH and CK with spectrophotometric methods. While a comparison between the kinetic properties of the LDH and CK of both groups revealed not only different Michaelis constants and optimum temperatures for LDH but also different Michaelis constants and optimum pH for CK, the pH profile of LDH and optimum temperature of CK were similar. In defining some kinetic properties of cardiac metabolic enzymes of ESC-derived cardiomyocytes, our results are expected to further facilitate the use of ESCs as an experimental model.

• 한국발생생물학회지:발생과생식
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• 제20권2호
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• pp.149-155
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• 2016

Sprouty (Spry) genes encode inhibitors of the receptor tyrosine kinase signaling cascade, which plays important roles in stem cells. However, the role of Spry4 in the stemness of embryonic stem cells has not been fully elucidated. Here, we used mouse embryonic stem cells (mESCs) as a model system to investigate the role of Spry4 in the stem cells. Suppression of Spry4 expression results in the decreases of cell proliferation, EB formation and stemness marker expression, suggesting that Spry4 activity is associated with stemness of mESCs. Teratoma assay showed that the cartilage maturation was facilitated in Spry4 knocked down mESCs. Our results suggest that Spry4 is an important regulator of the stemness and differentiation of mESCs.