Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Critical diagnostic and cancer stem cell markers in neoplastic cells from canine primary and xenografted pulmonary adenocarcinoma

  • Warisraporn, Tangchang (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • YunHyeok, Kim (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Ye-In, Oh (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Byung-Woo, Lee (Biotoxtech) ;
  • Hyunwook, Kim (Haemaru Animal Hospital) ;
  • Byungil, Yoon (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
  • Received : 2022.05.02
  • Accepted : 2022.09.13
  • Published : 2022.11.30
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Abstract

It is challenging to diagnose metastatic tumors whose cellular morphology is different from the primary. We characterized canine primary pulmonary adenocarcinoma (PAC) and its xenografted tumors by histological and immunohistochemical analyses for critical diagnostic and cancer stem cell (CSC) markers. To generate a tumor xenograft model, we subsequently transplanted the tissue pieces from the PAC into athymic nude mice. Immunohistochemical examination was performed for diagnostic (TTF-1, Napsin A, and SP-A) and CSC markers (CD44 and CD133). The use of CSC markers together with diagnostic markers can improve the detection and diagnosis of canine primary and metastatic adenocarcinomas.

Keywords

Acknowledgement

The first author, Warisraporn Tangchang, would like to express her gratitude for the financial support of the NVI Thailand and Naresuan University in Thailand.

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