• 대한기계학회논문집B
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• 제37권7호
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• pp.703-708
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• 2013

고관절 전치환술은 대퇴골두와 비구 부분이 손상되어 제 기능을 하지 못하였을 때, 인공 고관절로 대체하는 수술로서 인체에는 많은 양의 절개를 필요로 하며 장기간의 회복시간과 재활시간을 필요로 한다. 최소침습술은 환자에게 흉터와 근육절개를 최소화하고, 회복시간을 단축하여 미관상 흉터가 적어 가장 선호되는 수술 방법이다. 이 논문에서는 최소침습술이 가능한 메커니컬 스템(Mechanical stem)을 기어의 원리를 이용하여 개발하였다. 재치환술을 할 경우에는 스템 전체를 교체할 필요 없이 일부 부품만을 교체하여 수술 시간을 단축할 수 있도록 설계하였으며, 아크릴 소재로 모형을 제작하여 작동에 문제가 없는 것을 확인하였다. 3D Bio-CAD 모델링 기법을 통하여 메커니컬 스템의 기하학적 모델링을 하였다. 실험 대상자의 고관절 부위는 컴퓨터 단층촬영 영상데이터를 기반으로 3차원 재구축을 하였고, 가상 시술 환경 조건에서 몸무게보다 더 과중한 하중을 적용하여 메커니컬 스템의 안전성을 유한요소해석으로 확인하였다.

• 대한의생명과학회지
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• 제13권4호
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• pp.273-278
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• 2007

Stem cells have been the subject of increasing scientific interest because of their utility in numerous biomedical applications. Stem cells are capable of renewing themselves; that is, they can be continuously cultured in an undifferentiated state, giving rise to more specialized cells of the human body. Therefore, stem cells are an important new tools for developing unique, in vitro model systems to test drugs and chemicals and a potential to predict or anticipate toxicity in humans. In the present study, in vitro cultured F3 immortalized human neural stem cell line and in vivo adult Sprague Dawley rats was used to evaluate the cytotoxicity of anticancer drug paclitaxel. In vitro apoptotic activity of paclitaxel was evaluated in F3 cell line by a MTT assay and DAPI test. The cell death was induced with the treatment of 20 nM paclitaxel and chromatin degradation was detected by DAPI staining, which was analyzed by fluorescent microscope. In vivo studies, we also observed nestin immunoreactivity on subventricular zone, which is stem cell rich region in the adult brain of the SD rat. Immunofluorescent staining result shows that pixel intensities of nestin were decreased in a dose dependent manner. These results suggest that paclitaxel is able to induce cytotoxic activity both in F3 neural stem cell line and neural stem cell in SD rat brain.

• 한국기후변화학회지
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• 제8권4호
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• pp.415-424
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• 2017

This study was conducted to estimate carbon stocks of Pinus densiflora with drawing volume of trees in each tree height and DBH applying the suitable stem taper equation and tree specific carbon emission factors, using collected growth data from all over the country. Information on distribution area, tree age, tree number per hectare, tree volume and volume stocks were obtained from the $5^{th}$ National Forest Inventory (2006~2010) and Statistical yearbook of forest (2016), and method provided in IPCC GPG was applied to estimate carbon stock and uptake. Performance in predicting stem diameter at a specific point along a stem in Pinus densiflora by applying Kozak's model, $d=a_{1}DBH^{a_2}a_3^{DBH}X^{b_{1}Z^2+b_2ln(Z+0.001)+b_3\sqrt{Z}+b_4e^z+b_5(\frac{DBH}{H})}$, which is well known equation in stem taper estimation, was evaluated with validations statistics, Fitness Index, Bias and Standard Error of Bias. Consequently, Kozak's model turned out to be suitable in all validations statistics. Stem volume table of P. densiflora was derived by applying Kozak's model and carbon stock tables in each tree height and DBH were developed with country-specific carbon emission factors ($WD=0.445t/m^3$, BEF = 1.445, R = 0.255) of P. densiflora. As the results of analysis in carbon uptake for each province, the values were high with Gangwon-do $9.4tCO_2/ha/yr$, Gyeongsandnam-do and Gyeonggi-do $8.7tCO_2/ha/yr$, Chungcheongnam-do $7.9tCO_2/ha/yr$ and Gyeongsangbuk-do $7.8tCO_2/ha/yr$ in order, and Jeju-do was the lowest with $6.8tC/ha/yr$. Total carbon stocks of P. densiflora were 127,677 thousands tC which is 25.5% compared with total percentage of forest and carbon stock per hectare (ha) was $84.5tC/ha/yr$ and $7.8tCO_2/ha/yr$, respectively.

• 한국항해학회지
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• 제17권1호
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• pp.37-48
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• 1993

When the vessel is running at the very low Froude numbers, the free-surface is difficult to be disturbed, wave-making is negligible, and the double -model velocity potential gives a very good approximation for calculating the velocity distribution just outside the boundary layer. If the speed of incident flow is gradually increased, the most perceptible change is the rise of the flow surface at stem. With further increase in speed, the nature of the flow at the bow changes completely, The flow ahead of the bow becomes more distrubed, the rise at the stem to stagnation height disappear, and the first wave crest, of less than the stagnation height, appears a small distance downstream from the stem. The present study is concerned with a small region of this flow, mainly in the bow region. The present investigation is primarily an experimental study of the flow in the bow region of s ship model, and it is undertaken in order to investigated systematically, the effect of bow geometry on this flow. The long-range objective is to use these results to guide the development of a mathematical model for predicting the flow about a ship's bow.

• 한국해안해양공학회지
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• 제2권1호
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• pp.51-57
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• 1990

수심변화가 완만하고 흐름이 없는 곳을 파가 전파할 때 겪게되는 침수, 굴절 및 회절현상의 해석에는 3차 Stokes파 이론에 의한 선형, 비선형, 포물형 방정식이 이용되며, 여기서는 바닥마찰과 바람의 영향은 고려하지 않는다. 이 포물형 방정식으로 암초가 있는 경우에 대해 수치해석을 수행하여 기존의 실험치와 비교 검토하였고, 회절과 굴절효과의 중요성을 고찰했다. 천해파의 특성변화 해석에는 Boussinesq방정식에 기초한 포물형 방정식이 이용된다. 흐름이 없는 경우에 방파제를 따라 전파하는 Cnoidal파의 회절현상을 수심이 변하고 입사각이 변하는 경우에 대해 수치해석을 하여 Stem wave의 특성에 대해 논의하였다.

• BMB Reports
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• 제50권2호
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• pp.79-84
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• 2017

Emphysema, a pathologic component of the chronic obstructive pulmonary disease, causes irreversible destruction of lung. Many researchers have reported that mesenchymal stem cells can regenerate lung tissue after emphysema. We evaluated if spheroid human adipose-derived mesenchymal stem cells (ASCs) showed greater regenerative effects than dissociated ASCs in mice with elastase-induced emphysema. ASCs were administered via an intrapleural route. Mice injected with spheroid ASCs showed improved regeneration of lung tissues, increased expression of growth factors such as fibroblast growth factor-2 (FGF2) and hepatocyte growth factor (HGF), and a reduction in proteases with an induction of protease inhibitors when compared with mice injected with dissociated ASCs. Our findings indicate that spheroid ASCs show better regeneration of lung tissues than dissociated ACSs in mice with elastase-induced emphysema.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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• pp.74-74
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• 2003

Embryonic stem cells have several characteristics suitable for cell replacement therapy. To investigate a possibility of using human embryonic stem cell (hESC) as a carrier of therapeutic gene(s), hESC (MB03) was co-transfected with cDNAS coding for tyrosine hydroxylase (TH) and GTP cyclohydrolase Ⅰ (GTPCH Ⅰ) and bulk-selected using neomycin and hygromycin-B. Successful transfection was confirmed by western immunoblotting and RT-PCR. The genetically modified hESC (bk-THGC) relieved apomorphine-induced asymmetric motor behavior by approximately 54% when grafted into striatum of 6-OHDA-denervated rat brain. The number of rotation, however, increased up to 176+18% in 6 weeks when sham-grafted compared with number of rotation before graft. Immunohistochemical staining revealed that the grafted hESC survived and expressed TH for at least 6 weeks while the experiment was continued.

• Journal of Advanced Research in Ocean Engineering
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• 제2권2호
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• pp.67-80
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• 2016

The resistance performance of an icebreaking cargo vessel with varied stem angles is investigated numerically and experimentally. Ship-ice interaction loads are numerically calculated based on the fluid structure interaction (FSI) method using the commercial FE package LS-DYNA. Test results obtained from model testing with synthetic ice at the Pusan National University towing tank and with refrigerated ice at the National Research Council's (NRC) ice tank are used to validate and benchmark the numerical simulations. The designed icebreaking cargo vessel with three stem angles ($20^{\circ}$, $25^{\circ}$, and $30^{\circ}$) is used as the target ship for three concentrations (90%, 80%, and 60%) of pack ice conditions. The comparisons between numerical and experimental results are shown and our main conclusions are given.

• Advances in Traditional Medicine
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• 제7권1호
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• pp.46-50
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• 2007

Callus cultures of Convolvulus microphyllus Sieb. was induced on Murashige and Skoog's medium supplemented with 2,4-dichloro phenoxy acetic acid, 6-benzyl adenine, indole acetic acid and kinetin (1 ppm each). Methanolic extracts of whole plant, leaf, stem and leaf and stem calli were tested for anticonvulsant activity against standard drug phenytoin using maximal electroshock model on mice. It was observed that the animals treated with methanolic extracts of stem callus, leaf callus and whole plant (200 mg/kg, oral) showed significant protection against tonic convulsions induced by transcorneal electroshock. Anticonvulsant activity of methanolic extract of stem callus was comparable to that of standard drug phenytoin.

• 한국발생생물학회지:발생과생식
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• 제21권4호
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• pp.449-456
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• 2017

The germline stem cells of the Drosophila ovary continuously produce eggs throughout the life-span. Intricate regulation of stemness and differentiation is critical to this continuous production. The translational regulator Nos is an intrinsic factor that is required for maintenance of stemness in germline stem cells. Nos expression is reduced in differentiating cells at the post-transcriptional level by diverse translational regulators. However, molecular mechanisms underlying Nos repression are not completely understood. Through three distinct protein-protein interaction experiments, we identified specific molecular interactions between translational regulators involved in Nos repression. Our findings suggest a model in which protein complexes assemble on the 3' untranslated region of Nos mRNA in order to regulate Nos expression at the post-transcriptional level.