• Title/Summary/Keyword: stem culture

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Japanese and Chinese Journalists' Views on Anti-Korean Wave (일본과 중국 언론인들의 반한류 인식)

  • Kim, Eunjune;Kim, Sujeong
    • The Journal of the Korea Contents Association
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    • v.16 no.6
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    • pp.802-813
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    • 2016
  • This study examined the Japanese and Chinese journalist's views on anti-Korean wave, who are the public and authoritative discourse producers in Japan and China, respectively. In so doing, the study aims to understand the ways in which the phenomena of anti-Korean wave take place and are diffused. According to the findings, anti-Korean wave in north-east Asia is affected by anti-Korea sentiments that have been induced from historical and political relations as well as cultural conflicts. In specific, the anti-Korea sentiments found in both Japan and China are geopolitical particularity and historical relations function to frame their cultural receptions of Korean pop culture. In other words, the phenomena of anti-Korean wave in both countries do not stem directly from local audiences' either discontents or apathy on Korean pop contents. However, while Japanese anti-Korean wave seems to be mere expressions of anti-Korea sentiments, Chinese sentiments of anti-Korean wave are triggered and transferred by, or articulated with their anti-Korea sentiments.

In-vitro and In-vivo Biocompatibility Evaluation of Silica Based Bio-active Glass Prepared by Hydrothermal Method

  • Sarkar, Swapan Kumar;Nguyen, Phuong Thi;Lee, Byong-Taek
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2011.05a
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    • pp.53.1-53.1
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    • 2011
  • Bioactive glass powders were synthesized by hydrothermal chemical route by the use of ultrasonic energy irradiation. We used sodalime, calcium nitrate tetra hydrate and di ammonium hydrogen phosphate as the precursor material to synthesize $SiO_2$ rich bio-active glass materials. The $SiO_2$ content was varied in the precursor mixture to 60, 52 and 45 mole%. Dense compacts were obtained by microwave sintering at $1,100^{\circ}C$. Mechanical properties were characterized for the fabricated dense bioactive glasses and were found to be comparable with conventional CaO-$SiO_2$-$Na_2O$-$P_2O_5$ bioactive glass. Detailed biocompatibility evaluation of the glass composition was investigated by in-vitro culture of MG-63 cell and mesenchyme stem cell. Cell adhesion behavior was investigated for both of the cell by one cell morphology for 30, 60 and 90 minutes. Cell proliferation behavior was investigated by culturing both of the cells for 1, 3 and 7 days and was found to be excellent. Both SEM and confocal laser scanning microscopy were used for the investigation. Western blot analysis was performed to evaluate the bimolecular level interaction and extent and rate of specific protein expression. The ability to form biological apatite in physiological condition was observed with simulated body fluid (SBF). In-vivo bone formation behavior was investigated after implanting the materials inside rabbit femur for 1 and 3 month. The bone formation behavior was excellent in all the bioglass compositions, specially the composition with 60% $SiO_2$ content showed most promising trend.

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Evaluation of Certain Plant Extracts and Antagonists Against Fusarium solani and Alternaria tenuissima, the Incitants of Root Rot and Die-Back Diseases of Mulberry

  • Seetha, Ramulu J.;Raja, Gopal Reddy C.;Ramanjaneyulu, R.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.20 no.1
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    • pp.1-5
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    • 2010
  • The studies have been conducted to control the soil borne fungal pathogens viz, Fusarium solani (Mart) Sacc. and Alternaria tenuissima the incitants of root rot and die-back diseases on mulberry stem cuttings planted in the mulberry nurseries and also in established mulberry gardens ten plant extracts with 10% concentration except Lantana camara (undiluted) were tested through poisoned food technique and four biofungicides were also screened by dual culture method under in vitro conditions. Plant extract of Prosopis juliflora showed the maximum inhibition on the mycelial growth (81.2% over A. tenuissima and 80.0% over F. solani) and followed by L. camara (66.7% over A. tenuissima and 68.9% over F. solani). Among the antagonists Pseudomonas fluorescens and Trichoderma viride showed maximum inhibition on the mycelial growth of both pathogenic fungi. The promising plant extracts (P. juliflora and L. camara) and antagonists (P. fluorescens and T. viride) were tested against both the pathogenic fungi under in vivo conditions along with the existing popular chemical Mancozeb. All the tested plant products and bio-fungicides showed inhibitory effect on both fungi. But the maximum survival percentage of mulberry cuttings was recorded in the treatment with T. viride (95% against F. solani and 90% against A. tenuisssima) followed by P. fluorescens (90% against both fungi) and T. harzianum (80% against F. solani and 85% against A. tenuisssima). Incase of the treatments with plant extracts and chemical fungicide the P. juliflora (60% against F. solani and 55% against A. tenuisssima) showed higher survival percentage and followed by L. camara (55% against F. solani and 50% against A. tenuisssima) and Mancozeb (55% against both fungi). In case of control only 10% of survival was recorded in F. solani inoculated cuttings and 15% survival in A. tenuissima inoculated cuttings.

Relative Competitive Strengths of Overseas Chinese Firms in China: Advantages of Homeland Investment (중국투자 화교기업의 상대적 경쟁력: 모국투자의 이점)

  • Kim, Jong-Shik
    • Korean Business Review
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    • v.18 no.2
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    • pp.21-43
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    • 2005
  • Since China opened its market to foreign investors, overseas Chinese firms have been the biggest foreign investors. Because of the uncertain market environment, other foreign finns incurred larger liabilities of foreignness than overseas Chinese finns. Some parts of liabilities of foreignness stem from cultural differences and last for quite long time. Therefore better understanding of Chinese culture and guanxi network can give overseas Chinese finns competitive advantages over those from other countries by offering market information and reducing transaction costs. Also better management skills, information about export markets and abundant capital allow them to maintain competitive strengths over local firms. As foreign firms accumulate market knowledge in China, they can reduce liabilities of foreignness. However, it is very difficult for foreigners to understand Chinese cultures and to utilize guanxi network as well as Chinese. It is likely that Overseas Chines firms can have competitive strengths in some industries such as banking, retailing, and foreign trade, where relationship with local contractors and exploitation of worldwide Chines networks can play important roles to make successful business deals.

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The relationship between the sasangchaejil and the results of laboratory examinations (사상체질과 임상검사 결과와의 상관관계)

  • Lee, Yong-Koo;Sul, In-Chan
    • Journal of Haehwa Medicine
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    • v.8 no.2
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    • pp.383-397
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    • 2000
  • Background/Aims: Dong-Moo Lee Jae-Ma(1837-1900) confirmed the sasangchaejil theory based on the sasang-medicine. This theory has been the main-stem of oriental medicine during 100 years in Korea. But rarely anyone has tried to prove this theory systemicaly. So we have a trial to clarify any relationship between the sasangchaejil and the laboratory and clinical results of mass screening tests. Methods: We evaluated the laboratory values of 280 people who had taken many clinical and laboratory tests. The laboratory examinations were complete blood count, liver and kidney function test, ultrasonogram of the abdomen and breasts, gastrofiberscope, many tumor markers body mass index, blood pressure, glucose, and electrocardiogram, etc. Results: 1) Among the 280 subjects, 187/66.8% were sowumin, 58/20.7% were taewumin and 35/12.5% were soyangin. 2) Those who reacted positive to HBsAb were 22 subjects(62.8%) in soyangin, 57.2% in sowumin and 55.1% in raewumin. But there were no significant correlations. 3) 45 subjects(72.4%) in taewumin had obesity, fatty liver and hyperlipidemia.(P<0.05) 4) 20.6% in taewumin had abnormal LFT suggesting hepatic disease. It was statistically sigificant.(P<0.05) 5) An anemic state was present in women of soyangin and sowumin mostly. Since an iron deficiency anemia is common in women, there were no significant corelations among sasangchaejil. Also urinary tract infectons were common in women. 6) 6 subjects in taewumin(10.3%) had cardiac problems, while only one case(2.8%) occured in soyangin and 7 cases(3.7%) in sowumin. Taewumin was significantly high.(P<0.05) 7) In the case of gastric disease, there was no distinguishable difference among sasangchaejil.(taewumin 37.9%, soyangin 31.4% and sowumin 35.2% rrespectively) 8) There was no significant difference beteen subjects with different blood types using sasangchaejil. Conclusions: There were some significant relationship between sasangchaejil and diseases prevalent to them by the theory of sasang-medicine. But Lee didn't considered the differences of gender, the change of body status according to the development of culture, and circumstances of their lives. Also he didn't consider the existence of infectious agents. Now more systemic study with larger populations are requied.

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Effects of Artificial Zeolite and Various ion Exchangers Supplemented in Rockwool on the Ion Exchangability of Muskmelon (암면배지내 인공 zeolite 형 각종 이온교환제 처리가 온실멜론의 이온교환능에 미치는 영향)

  • 장홍기;정순주
    • Journal of Bio-Environment Control
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    • v.5 no.1
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    • pp.43-49
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    • 1996
  • This study was conducted to investigate the effects of artificial zeolite and various ion exchangers supplemented in rockwool on the ion exchangability of muskmelon. The results obtained were as follows ; Plant height was higher in the treatment of Ca type artificial zeolite as of 131.2cm than that of control as of 124cm, and same trends were shown in fresh weight of leaf and stem. Fruit weight was increased by supplement of artificial zeolite, but there were no significant differences in the sugar degree and external appearance as influenced by supplementing artificial zeolite. Supplement of artificial zeolite stabilized the pH and increased the ion exchange capacity of nutrient solution. Nutrient absorption was more favorable and led to growth promotion. This study was demonstrated that supplement of artificial zeolite in rockwool slab was improved the stabilization of root environment and increased ion uptake of muskmelon plant.

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Keratin 17 identified by proteomic analysis may be involved in tumor angiogenesis

  • Xu, Yong;Zhang, Su-Zhen;Huang, Can-Hua;Liu, Xin-Yu;Zhong, Zhen-Hua;Hou, Wen-Li;Su, Zi-Fen;Wei, Yu-Quan
    • BMB Reports
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    • v.42 no.6
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    • pp.344-349
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    • 2009
  • Angiogenesis is crucial for solid tumor growth. By secreting angiogenic factors, tumor cells induce angiogenesis. However, targeting these angiogenic factors for cancer therapy is not always successful, suggesting that other factors may be involved in tumor angiogenesis. This work shows that 25 protein spots were differentially expressed by two-dimensional gel electrophoretic analysis when HepG2 cells induced endothelial cell differentiation to tube in vitro, and most of them were upregulated. Twenty-one proteins were identified with MALDITOF-MS, and the other four were identified by LTQ-MS/MS. Keratins were identified as one class of these upregulated proteins. Further study indicated that the expression of keratin 17 in cultured endothelial cells is likely microenvironment regulated, because its expression can be induced by HepG2 cells and bFGF as well as serum in culture media. Increased expression of keratins in endothelial cells, such as keratin 17, may contribute to the angiogenesis induced by HepG2 cells.

Effects of Dessication, Sucrose and Salt Stress on the Regeneration of Portulaca oleracea Cultured Cells (건조, 염분 및 탕의 처리가 쇠비름(Portulaca oleracea L.) 배양세포의 재분화에 미치는 영향)

  • 권순태;오세명
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.2
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    • pp.117-121
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    • 1994
  • The optimal level of growth regulator for callus initiation stem explants was BAP 0.1mg/L combined with 2,4-D 1.0 mg/L in Murashige and Skoog (MS) medium supplemented with 30g/L sucrose and 10g/L agar, and that for cell growth was BAP 0.1+2,4-D 0.5 mg/L in MS liquid medium. The regeneration frequency of P. oleracea cells was significantly increased by subjecting the cells to dessication for 1and 2 h up to 83%, respectively, as compared with untreated control showing 61%. Cell viability and survival rate was inhibited by pretreatment of 0.6% NaCl for 2 days, while regeneration ability was not affected by the treatment. Pretreatment of 100g/L sucrose for 2 days markedly stimulated the regeneration of cells up to 81%. These results suggest that in addition to physiological changes, water stress resulted from dessication and high concentration of sucrose and NaCl is closely related to the regeneration of P. oleracea cultured cells.

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Introduction of Maize Transposable Elements, Ac and Ds into the Genome of a Diploid Potato Species (옥수수 전위유전자 Ac 및 Ds의 2배체종 감자 Genome 내로의 도입)

  • 김화영;임용표
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.1
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    • pp.39-45
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    • 2000
  • Two maize transposable elements, immobilized Ac (iAc) and Ds, have been introduced into the genome of a diploid potato clone (Solanum tuberosum Group Phureja clone 1.22). The iAc is a modified Ac that is supposed to be unable to transpose but is expected to trans-activate the transposition of a Ds that is unable to transpose by itself. When the leaf and stem explants of in vitro shoots of the clone 1.22 were inoculated with Agrobacterium tumefaciens strains harboring binary vectors containing the iAc and the Ds, calli were formed from the explants on media containing 50 mg/L of kanamycin, and shoots were regenerated from the calli. The regenerated shoots formed roots when cultured on media containing 100 mg/L of kanamycin, whereas untransformed shoots did not form roots on the same media. The PCR amplification of the DNA's from the transgenic plants confirmed that the iAc and the Ds elements were introduced into the potato genome of 1.22.

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Plant Regeneration via Somatic Embryogenesis and Organogenesis from Peucedanum japonicum $T_HUNB$ (갯기름나물의 체세포배 분화와 기관 분화를 통한 재분화)

  • Kim, Ok-Tae;Kim, Kwang-Soo;An, Jun-Chul;Hwang, Baik
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.1
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    • pp.21-24
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    • 2001
  • Peucedanum japonicum $T_{HUNB}$ used as a edible and medicinal plants was investigated for in uitro regeneration. Callus formation occurred on leaf and stem explant cultures and showed spontaneous embryogenic and organogenic capability on MS basal medium supplemented with 0.1~5 mg/L NAA and 0~10 mg/L BA in dark. The regeneration was highest on the condition supplemented with 2.5 mg/L NAA and 10 mg/L BA. Development of the somatic embryo progressed through the globular, heart-shaped, torpedo-shaped and cotyledonary stage, typical of zygotic embryos. When the first somatic embryos was cultured on the medium supplemented with 0.2 mg/L NAA, secondary somatic embryo were induced with higher frequency on the hypocotyl then on the cotyledon and root.t.

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