• Journal of Plant Biotechnology
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• v.4 no.1
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• pp.17-21
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• 2002

To establish an the mass production system of grape anthocyanin pigments through callus and cell suspension culture, the effects of various combinations of auxins and cytokinins on friable callus production were studied. for friable callus production, 2,4-D was superior to other regulators. IAA at 2 mg/L induced callus from stem and petiole while NAA resulted in rooting. Callus induction rate increased with the 2,4-D level, and stem segments were superior to leaf blade or petiole, showing nearly 100% with 1 and 2 mg/L 2,4-D from petiole and stem. Combined treatments of 2,4-D + kinetin and NAA + BA also yielded friable callus from stem segments. In treatments with 1 mg/L 2,4-D + 1 mg/L kinetin and 1 mg/L NAA + 1 mg/L BA, callus induction rate was nearly 100%. The combination effect of 2,4-D and BA on anthocyanin production was not significant.

• Korean Journal of Plant Resources
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• v.14 no.2
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• pp.116-123
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• 2001

The non-enzymatic antioxidants and antioxidant enzyme from the extracts of Parthenocissus tricuspidata PLANCH. were examined in order to utilize natural product for cerchemopreventive agents. The antioxidant potential and enzyme activities on plant positions in the extracts of Parthenocisuss tricuspidata PLANCH. showed considerable differences. The antioxidant activity of the leaf extracts by Ethyl acetate fractions of Parthenocisuss tricuspidata PLANCH. was the highest among three positions ($7.57\mu\textrm{g}/m\ell$). The highest activities showed in S-5 (in leaf), S-4 (in stem) and S-3 (in root) fraction by Silicagel column chromatography and the antioxidant activity showed, in purified extract of each positions, $7.06\mu\textrm{g}/m\ell$ (in leaf), $6.99\mu\textrm{g}/m\ell$ (in stem) and $12.39\mu\textrm{g}/m\ell$ (in root) respectively. The activities of DPPH by LH-20 column chromatography revealed much higher than those by silica-gel column chromatography. These were identified as the phenolic compounds known as antioxidant compounds such as Benzoic acid(Gallic acid), 1-methyl-3-(2-phenylethen) benzene, phloroglucinol and 1,2-dihydroxy-4-(1-propyl)benzene by GC/MS. POD activities in the stem and root were higher than in the leaf. SOD activity was highest in the leaf, stem and root activity was comparatively low. Especially, SOD activity in leaf was over 2 times higher than root.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.24 no.3
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• pp.81-84
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• 1979

Present studies were carried out to clarify the variation of agronomic characters of Panax ginseng (violet-stem variant and yellow-berry variant) and Panax quinquefolium (American ginseng). The variations of stem diameter. stem length, leaf width, root diameter. and root length of Panax quinquefolium were found to be more variable as compared with those of Panax ginseng. As for the variations of agronomic characters of Panax ginseng. violet-stem variant was more variable than yellow-berry variant. In Panax ginseng as well as Panax quinquefolium . the variability of stem length. leaf length, and root length was high. while the other agronomic characters showed comparatively low variability.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.1
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• pp.16-25
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• 1995

The effects of leaf removal time on variation of nodal sink components in determinated soybean ［Glycine max （L.） Merr.］ cultivar 'Danyeobkong' were measured at the experiment field of Chonbuk Provincial Rural Development Administration in 1991. Node order in this experiment was calculated from terminal node to bottom node to clarify the photosynthetic ability of canopy leaves. The upper 5 leaves from terminal node of main stem were removed at 5, 15, 25, 35 and 45 days after flowering(DAF) respectively. In spite of light-receiving increment in lower part of main stem and in branch by removing the upper part leaves of main stem, seed weight of that part was not increased in leaf removal treatment compared with control block. The node position reducing pod went down from terminal to bottom by delaying leaf removal time, and the influence of pod number reduction was bigger in DAF 25 than in DAF 15. But the seed number reduction per pod was the biggest in DAF 35. Cracked seed coat ratio ranged from 25% to 35% in control block, while that of leaf removal block had mostly 10% gap compared with the control block.rol block.

• Proceedings of the Korean Fiber Society Conference
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• 1996.10a
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• pp.500-506
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• 1996

To study antimicrobial activities of Impatiens balsamina, its leaf and stem was extracted by methanol and water. The extracts were tested their microbial inhibition activities against several microorganisms, staphylococcus aureus KCTC 1928, Escherichia coli KCTC 1116, Klebsiella pneumoniae KCTC 2690, Trichophyton mentagrophytes KCTC 6316. The methanol extract and water extract of the leaf and stem didn't show the inhibition effect on E. coli but showed the inhibition effect against the ohter 3 species of the microorganisms tested. The extract of leaf showed higher inhibition than the extract of stem. And the methanol extract showed higher inhibition than the water extract.

• Food Science and Biotechnology
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• v.14 no.2
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• pp.177-180
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• 2005

Antioxidant activity of crude extracts from colored sweet potato cultivars by plant parts such as root, stem and leaf was evaluated. The highest TBARS values were obtained from root samples of sweet patato, and followed by stems and leaves, indicating that leaf sample showed the strongest antioxidant activity. Sweet potato cultivars with yellow flesh and leaf part exhibited strong antioxidant activities. Antioxidant activities of leaf and stem extracts were maintained for 21 days and were a little lower than that of BHT. The DPPH radical scavenging activity was the highest in "Sinhwangmi" leaf, and followed by "Jami" root. Chlorogenic acid was detected as the most abundant antioxidant substance among all fractions. These results suggest that the antioxidant activity of sweet potato differs depending on plant part and cultivar.

• Journal of Life Science
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• v.8 no.6
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• pp.623-626
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• 1998

Conditions for somatic embryogenesis and plant regeneration from stem tissues of Euphorbia pulcherrima were esta-blished. Explants from leaf, petiole, stem were examined for their embryogenesis on MS solid medium supplemented with plant growth hormones in combination at various concentrations. From leaf or petiole explants, callus was indu-ced well but never proceeded to the embryonic stage in our expermental conditions. From stem explants, however, multiple shoots following callus induction emerged in about 6 to 8 weeks on MS agar medium supplemented with 1.5 mg/L of benzyladenine. Upon transfer, roots were developed on hormone-free MS solid medium.

• Korean Journal of Plant Resources
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• v.7 no.1
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• pp.63-66
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• 1994

The experiment was carried out to study the bothanical characteristics of Aconitum charmichaeli Sieb. et Zucc in Korea.The results obtained are summarized as follows ; maximum flowering date was 15 Sep. and plantheight was 65cm, stem diameter was 8.7mm, No. of node was 25 and node of flowering initiation was15th.In flowering, No. of anothers were 52 and No. of flowers were 49.In leaf, length and width was same size, lower leaf size was 2-3 times than upper leaf, lower leafarea was 8.4 times than upper leaf.In tuber, No. of tuber per plant was 8, fresh weight per tuber was 25.4g, total fresh weight per plantwas about 200g.

• Korean Journal of Medicinal Crop Science
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• v.10 no.4
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• pp.259-262
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• 2002

The efficiency of regeneration of callus and explants from leaf and stem disks of Epimedium koreanum was examined on the MS media containing 2,4-D, NAA, Kinetin, BA and TDZ. Calli were formed on the 2mg/l 2,4-D media at the rate of 32% from leaf discs and 52% from stems. No callus was produced on the media which are containing BA or TDZ alone. The combination of 2,4-D and BA showed the effect on the formation of callus. The combination of 2mg/l 2,4-D and 0.lmg/l BA in the MS media had produced the highest percentage of callus formation, 50% from leaf discs and 40% from stems, respectively. The combination of 2mg/l 2,4-D and 1mg/1 BA in the MS media had affected the formation of callus in the rate of 40% from leaf discs and 25% from stems. The combined plant growth regulators of 2,4-D and BA increased the formation of calli from leaf discs, but single treatment of 2,4-D showed the highest callus formation from stems. Multiple shoots from leaf discs were formed on the media containing NAA, BA, kinetin, and TDZ. The highest number of multiple shoots were obtained 0.1mg/l NAA combined with 1mg/l kinetin. As a result, leaf discs or stems can be used for the mass propagation of Epimedium koreanum, but stem elongation of shoots from calli was not easy.

• Journal of Ecology and Environment
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• v.45 no.3
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• pp.143-151
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• 2021

Background: Plants are able to optimize defense responses induced by various herbivores, which have different feeding strategies. Local and systemic responses within a plant after herbivory are essential to modulate herbivore-specific plant responses. For instance, leaf-chewing herbivores elicit jasmonic acid signaling, which result in the inductions of toxic chemicals in the attacked leaf (tissue-specific responses) and also in the other unattacked parts of the plant (systemic responses). Root herbivory induces toxic metabolites in the attacked root and alters the levels of transcripts and metabolites in the unattacked shoot. However, we have little knowledge of the local and systemic responses against stem-boring herbivores. In this study, we examined the systemic changes in metabolites in the wild tobacco Nicotiana attenuata, when the stem-boring herbivore Trichobaris mucorea attacks. Results: To investigate the systemic responses of T. mucorea attacks, we measured the levels of jasmonic acid (JA), JA-dependent secondary metabolites, soluble sugars, and free amino acids in 7 distinct tissues of N. attenuata: leaf lamina with epidermis (LLE), leaf midrib (LM), stem epidermis (SE), stem pith (SP), stem vascular bundle (SV), root cortex with epidermis (RCE), and root vascular bundle (RV). The levels of JA were increased in all root tissues and in LM by T. mucorea attacks. The levels of chlorogenic acids (CGAs) and nicotine were increased in all stem tissues by T. mucorea. However, CGA was systematically induced in LM, and nicotine was systematically induced in LM and RCE. We further tested the resource allocation by measuring soluble sugars and free amino acids in plant tissues. T. mucorea attacks increased the level of free amino acids in all tissues except in LLE. The levels of soluble sugars were significantly decreased in SE and SP, but increased in RV. Conclusions: The results reveal that plants have local- and systemic-specific responses in response to attack from a stem-boring herbivore. Interestingly, the level of induced secondary metabolites was not consistent with the systemic inductions of JA. Spatiotemporal resolution of plant defense responses against stem herbivory will be required to understand how a plant copes with attack from herbivores from different feeding guilds.