• Progress in Medical Physics
• /
• v.28 no.4
• /
• pp.156-163
• /
• 2017

To verify the usefulness of a steam sterilizer equipped with an electronic Bowie-Dick test system, this study was carried out using two methods, utilizing both a steam sterilizer and an electronic Bowie-Dick tester. The first method is to confirm the error detection of the chemical Bowie-Dick test pack and the electronic Bowie-Dick tester in a malfunctioning sterilizer environment. For this purpose, the Bowie-Dick test program for the steam sterilizer was used to test three types of test packs commonly used in hospitals and the electronic Bowie-Dick tester by changing the set values of temperature, time, and vacuum frequency. The second is an experiment to check the sterilizer's normal operation with the electronic Bowie-Dick tester and the usefulness of grasping the cause of the malfunction. The results showed that the sterilization temperature was the same as that of the test pack at a temperature $1{\sim}6^{\circ}C$ lower than the reference temperature of $134^{\circ}C$. In the test with the sterilization exposure time as a variable, there was a normal discoloration at a time difference of 30~90 s. In the experiment using the number of vacuum cycles, the test was correct by performing the normal discoloration only at the normal condition of 3 times. The test results of 30 hospitals were 100 failure tests by a total of 291 Bowie-Dick tests. Of these, the failure factors related to an internal temperature that the chemical test packs could not detect were the greatest, and the four factors related to temperature, including the internal temperature, were found to be 71.18% of total malfunctions. In addition, the Bowie-Dick tester was provided within 30 min after the start of the Bowie-Dick test to confirm the performance of the sterilizer and the detailed cause. A steam sterilizer equipped with an electronic Bowie-Dick test system is used to manage individual sterilizers. In the current steam sterilizer with many temperature-related errors, it is possible to check the malfunction of the temperature difference that the test pack cannot detect, and the cause of error for the sterilizer is immediately detected after the test. The steam sterilizer equipped with the electronic Bowie-Dick test system assists with infection control with accurate sterilizer performance assurance.

• Korean Journal of Organic Agriculture
• /
• v.9 no.4
• /
• pp.71-93
• /
• 2001

Within the paper, an overview of organic farming in Europe countries is given and the Principle and skills of organic agriculture is shortly reported with special regard to Germany. The overview information on European organic forming is covered such as \circled1 development of organic farming, \circled2organic farming organizations, \circled3standards and certification, \circled4implementation of EU council regulation, \circled5state support, \circled6implementation of Agenda 2000, \circled7training and education, \circled8advisory service and research situation. In the paper the principle and skills for organic farming which are practiced actually in the German organic farms is also reported. How to maintain and increase the fertility and microbiological activity of the soil by \circled1cultivation of legumes, green manures or deep-rooting crops in multi-annual rotation system, \circled2incorporation in the soil organic material, by-products from livestock farming is one of the major principle to organic crop production. Pest and diseases and weeds are controlled by any one, or a combination of the following measure ; \circled1choice of appropriate species and varieties, \circled2appropriate rotation programs, \circled3mechanical cultivation, \circled4protection of natural enemies of pests through provision of favourable habitat and ecological buffer zone, \circled5diversified ecosystems, \circled6flame weeding, \circled7natural enemies, \circled8bio-dynamic preparations, \circled9mulching and mowing, \circled10grazing of animals, ⑪mechanical controls, ⑫steam sterilization.

• Journal of Korean Clinical Nursing Research
• /
• v.25 no.3
• /
• pp.333-341
• /
• 2019

Purpose: The purpose of this study was to determine the most appropriate shelf life for sterilized products according to their packaging material. Methods: Samples were prepared to target six nursing units in one general hospital in Seoul. After steam and E.O gas sterilization, sterilized product, samples were supplied to wards. Data collection was conducted for 3 months, after the expiration date of 3 months had passed for samples packaged with crepe paper and nonwoven wraps. For samples packaged with paper-plastic pouches, data collection conducted for 3 months when the expiration date of 9 months had passed. The sterilized products were collected and tested for microbial contamination. Identification of the storage environment was done as samples were collected. Results: This study confirmed that the storage environment met international standards such as CDC, except for temperature. For steam sterilized crepe paper packaging samples and steam and E.O gas sterilized for nonwoven packaging samples no contamination in all products was found for 3 months past the expiration date. However, in the E.O gas sterilized paper-plastic pouch packaging sterile samples, Gram-positive bacilli were detected in one sample from a surgical intensive care unit at 45 weeks and another sample from an operating room at 47 weeks. Furthermore, the results did not show any microorganisms for up to 52 weeks in all products. Conclusion: According to the results of this study, sterilized product packaging made with crepe paper and nonwoven wraps is better able an extended shelf life from 3 months to 6 months, reducing unnecessary costs.

• Korean Journal of Acupuncture
• /
• v.22 no.2
• /
• pp.127-149
• /
• 2005

This study is designed to investigate the method of manufacturing herbal acupuncture through literature of oriental medicine. The findings of this study are as follows; 1. The methods of manufacturing herbal acupuncture go through the process of abstraction, purification, mixing, filtration, putting and tight sealing in the container, sterilization, quality control, printing and packing 2. There are many ways to manufacturing herbal acupuncture, for example water-alcohol precipitation, alcohol-water precipitation, liquid-liquid abstract, acid-base abstract, metal base precipitation, distillation, molecular structure, polyamide absorption, dialysis, and ion exchange, etc. And popular method is water-alcohol precipitation. This is through alcohol precipitate extracting the principal ingredients from water abstraction. This is very simple and efficient way using melting characteristics of compounds in herb to water and ethanol. 3. Sterilization of herbal acupuncture is through heating-pressure, boiling, steam flowing, low temperature, filtering, radiation, cooling, and microwaves. Nowadays filtering is commonly used. And sterilization is estimated by an examination of asepsis . 4. Herbal acupuncture must be undergo study and experiment to clinical use. The problems of herbal acupuncture are turbidity, instability, causing hemolysis, pain, and fever. So many provisions (addition, sterilization, and filtration etc.) must be prepared. 5. The theory of manufacturing herbal acupuncture is from oriental medicine, not western. So it must be corresponded to oriental medical theory, for example Gimi(氣味), Guigyung(歸經), Ingyung(引經), Bosa(補瀉), and Match of Herb. It is recommended that further study of many other sided investigations in the future.

• Journal of Microbiology and Biotechnology
• /
• v.27 no.7
• /
• pp.1209-1215
• /
• 2017

Microwave sterilization was performed to inactivate the spores of biofilms of Bacillus cereus involved in foodborne illness. The sterilization conditions, such as the amount of water and the operating temperature and treatment time, were optimized using statistical analysis based on 15 runs of experimental results designed by the Box-Behnken method. Statistical analysis showed that the optimal conditions for the inactivation of B. cereus biofilms were 14 ml of water, $108^{\circ}C$ of temperature, and 15 min of treatment time. Interestingly, response surface plots showed that the amount of water is the most important factor for microwave sterilization under the present conditions. Complete inactivation by microwaves was achieved in 5 min, and the inactivation efficiency by microwave was obviously higher than that by conventional steam autoclave. Finally, confocal laser scanning microscopy images showed that the principal effect of microwave treatment was cell membrane disruption. Thus, this study can contribute to the development of a process to control food-associated pathogens.

• Korean Journal of Soil Science and Fertilizer
• /
• v.23 no.2
• /
• pp.146-151
• /
• 1990

The viability of rhizobia according to various kinds of carrier materials, inoculum size, storage temperature and sterilization methods was investigated for the development of legume inoculat. The results were followings. 1. Peat and perlite were favorable as a carrier material. 2. Rhizobia counts were reached to $5{\times}10^8cells/g$ carrier 1-2 weeks after inoculation with inoculum size below $10^4cells/g$ carrier. 3. $10^9cells/g$ carrier was maintained 12 weeks after storage at room temperature. 4. Steam heat sterilization was the best method for carrier sterilization among methods used in this study. Dry heat and ${\gamma}$-ray sterilization were also applicable.

• Proceedings of the Korean Society for Bio-Environment Control Conference
• /
• 2006.05a
• /
• pp.70-75
• /
• 2006

• Journal of Environmental Health Sciences
• /
• v.8 no.1
• /
• pp.25-30
• /
• 1982

The incidence of hospital infection has been seriously increased in the general hospital in recent years. This study was performed on hospital air and materials in a General Hospital in Seoul from June to December in 1980. The results were as follows: 1. Air sampling was done in multiple strategic areas by exposing standard petridishes for 5 minutes. There was a significant difference of airborne microbe between places. ($F._{99}$ = 3.2, p < 0.01). 2. The mean colony count was 8.6$\pm$6.2 colonies / plate / 5 minutes. 3. Gram stains of colony in air sampling were Gram (+) cocci 66.5%. Gram (+) rod 18.4%, Gram (-) cocci 1.3%, Gram (-) rod 8.7% Fungus 4.5%. 4. For the evaluation of sterilization of steam sterilizer and ethylene oxide gas sterilizer, biological monitoring were done by commercial spore strip. Positive culture was obtained in 2 out of 41 tests on 3 steam sterilizers, and in 3 out of 13 tests on ethylene oxide gas sterilizer. 5. Product sampling and culture were done for 2 kinds of disinfectants and 30 sets of various operation package or dressing materials. Positive culture was obtained in one disinfectants.

• Journal of Industrial Technology
• /
• v.3
• /
• pp.87-94
• /
• 1983

Heating characteristics of red pepper paste packed in report pouches of various thicknesses and their storage stability were investigated to determine the adequate processing conditions that good bacteriological safty and minimal quality changes could be obtained, when sterilized by using a steam-air system retort. A heat penetration into pouch-packed red pepper paste was carried out through by a conductive heat transfer, indicating a simple logarithmic heating curve, and the smaller thickness revealed the higher heat penetration rate, suggesting the possibility of high temperature-short time sterilization of red pepper paste. The processing conditions with Fo-value of 4.5 or higher were sufficient for keeping up bacterial safty, but based on C-value, better quality retention was obtained at pouch thickness of 15mm under the processing temperature of $120^{\circ}C$. Subsequent storage study revealed that the red pepper paste packed in 15mm and processed at $120^{\circ}C$ with Fo=4.5 could be held without any spoilage and overall acceptance change, when stored for 6 months at room temperature under the relative humidity of 70%. After 3 months storage in $38^{\circ}C$ under saturation humidity, overall acceptance of red pepper paste were judged not to be maintainable on the acceptable level, but it may be suggested that above the results could be kept up a desirable quality without any remarkable deterioration.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.53 no.3
• /
• pp.316-325
• /
• 2020

The purpose of this study was to optimum formulation conditions for the preparation of retort pouched marsh snail Senisulcospira livertina soup with curled mallow Malva verticillata and optimized the sterilization process. Samples were prepared according to the central composite rotatable design (CCRD) by encrypting the amounts of marsh snail broth, boiled marsh snail and blanched curled mallow, which are the main ingredients of retort pouched marsh snail soup with curled mallow. Raw marsh snail was sorted by size of 4-5 level, and then marsh snail was washed and removed sediment in a water tank for 3 min, then boiled at 100℃ for 10 min, and then directly separated by hand to prepared a boiled marsh snail. The marsh snail cube is prepared by combining blanched at 100℃ for 10 sec curled mallow (27 g) and green onion (20 g) with boiled marsh snail (27 g) and soybean paste (10 g). The marsh snail cube was placed with marsh snail broth (416 g) in a retort pouch film. Subsequently, sterilization (118.0℃, 25 min; F₀ value, 12 min) and cooling were performed using a steam retort.